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47 results for “fungal symbionts”
Demographic data from long-term symbiont removal experiments with grasses and Epichloë fungal endophytes
This project was designed to understand the demographic effects of vertically transmitted fungal endophytes (Epichloë spp.) on their grass hosts. The experiment includes seven host-symbiont taxonomic pairs: Agrostis perennans - E. amarillans, Elymus villosus - E. elymi, Elymus virginicus - E. elymi or EviTG-1, Festuca subverticillata - E. starrii, Poa alsodes - E. alsodes, Poa sylvestris - E. PsyTG-1, Schedonorus arundinaceus - E. coenophiala. Experimental plots were established at the Indiana University Lilly-Dickey Woods Research and Teaching Preserve in south-central Indiana, USA in 2007. For each species, 5-10 plots were planted with naturally symbiotic (S+) hosts, and 5-10 plots were plated with hosts that were disinfected of fungal endophytes by heat treatment (S-). Over 15 years (2007-2022) we collected demographic data on the survival, growth, reproduction, and recruitment of all plants in all plots. Beginning in 2018 we also collected data on the locations of all plants in every plot.
Data from: Plant host traits mediated by foliar fungal symbionts and secondary metabolites
<p>Fungal symbionts living inside plant leaves ("endophytes") can vary from beneficial to parasitic, but the mechanisms by which the fungi affect the plant host phenotype remain poorly understood. Chemical interactions are likely the proximal mechanism of interaction between foliar endophytes and the plant, as individual fungal strains are often exploited for their diverse secondary metabolite production. Here, we go beyond single strains to examine commonalities in how 16 fungal endophytes shift plant phenotypic traits such as growth and physiology, and how those relate to plant metabolomics profiles. We inoculated individual fungi on switchgrass, <em>Panicum virgatum</em> L. This created a limited range of plant growth and physiology (2–370% of fungus-free controls on average), but effects of most fungi overlapped, indicating functional similarities in unstressed conditions. Overall plant metabolomics profiles included almost 2000 metabolites, which were broadly correlated with plant traits across all the fungal treatments. Terpenoid-rich samples were associated with larger, more physiologically active plants and phenolic-rich samples were associated with smaller, less active plants. Only 47 metabolites were enriched in plants inoculated with fungi relative to fungus-free controls, and of these, LASSO regression identified 12 metabolites that explained from 14–43% of plant trait variation. Fungal long-chain fatty acids and sterol precursors were positively associated with plant photosynthesis, conductance, and shoot biomass, but negatively associated with survival. The phytohormone gibberellin, in contrast, was negatively associated with plant physiology and biomass. These results can inform ongoing efforts to develop metabolites as crop management tools, either by direct application or via breeding, by identifying how associations with more beneficial components of the microbiome may be affected.</p>
Data from: Fungal symbionts generate water-saver and water-spender plant drought strategies via diverse effects on host gene expression
<p><em>Panicum</em> <em>hallii</em> var <em>hallii</em> HAL2 plants were inoculated individually with six foliar fungal endophytes or fungus-free controls and subjected to 5% or 20% soil moisture treatments. The fungi were selected for their previously observed effects on plant drought physiology, inducing either a "water saver" or a "water spender" strategy in the host. Plants were grown in enclosed microcosms to prevent cross-contamination and each treatment and control included 6 replicates. All fungi were Ascomycetes isolated from plants in central Texas. Plants were monitored for height, wilt, water loss, and survival. At the harvest, we also measured biomass and leaf colonization by the fungi and flash-froze leaf tissue for transcriptomic analyses. Both plant response and gene expression data are provided.</p>
Data from: Fungal symbionts generate water-saver and water-spender plant drought strategies via diverse effects on host gene expression
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Data from: Plant host traits mediated by foliar fungal symbionts and secondary metabolites
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Data from: Comparative phylogeography, genetic differentiation, and contrasting reproductive modes in three fungal symbionts of a multipartite bark beetle symbiosis
Multipartite symbioses are complex symbiotic relationships involving multiple interacting partners. These types of partnerships provide excellent opportunities in which to apply a comparative approach to identify common historical patterns of population differentiation and species-specific life history traits. Using three symbiotic blue stain fungal species (Ophiostomatacea) associated with outbreaking populations of the mountain pine beetle (Dendroctonus ponderosae Hopkins) in western Canada, we applied phylogenetic, population genetic, and demographic approaches to clarify phylogeographic patterns among the three fungal species. Broadly, the three species showed significant population differentiation, forming northern and southern populations, despite dramatic differences in haplotype diversity. Finer scale structuring and population demographic patterns were less consistent, showing some interspecific incongruence. By contrasting these species simultaneously, we were able to identify differences in recombination rate and ecological traits that can explain the observed patterns of incongruence among the fungal species. By applying a comparative approach to partners of a multipartite symbiosis we were able to distinguish congruent population structuring and species-specific differences that help us to understand the complexity and evolution of this symbiotic system.
Data from: A heritable symbiont and host-associated factors shape fungal endophyte communities across spatial scales
1. Although microbial ecologists are intensely interested in the processes governing microbial community assembly, progress has been limited by a lack of studies that span multiple geographical scales and levels of biological organization. 2. We used high throughput sequencing to characterize foliar fungal endophyte communities and host plant genetic structure both within, and among, 24 populations of spotted locoweed (Astragalus lentiginosus) across the Great Basin Desert. 3. Across the Great Basin, both within, and among populations of the host plant, fungal endophyte richness was predicted by plant size and variation in the seed-borne, heritable fungus, Alternaria fulva, which produces the bioactive alkaloid swainsonine. 4. The degree of between-plant turnover in the endophyte community was inversely related to host plant inbreeding and average plant size, and positively related to the relative abundance of A. fulva. Plant size was inversely related to endophyte community richness, both among, and within populations. The genetic and physical distance between host populations was not predictive of differences in fungal community structure. 5. Synthesis: Through pairing intensive local- and regional sampling, we uncovered a primacy of deterministic forces imposed by a heritable symbiont on the community structure of locoweed endophytes.
Fungal symbiont diversity drives growth of Holcus lanatus depending on soil nutrient availability
<ol> <li>Arbuscular mycorrhizal (AM) fungi frequently colonise plant roots and can affect plant morphology and physiology through their contribution to plant nutrition. However, the functional role of AM fungi in the presence of other microbial symbionts, including widespread Mucoromycotina 'fine root endophytes' (MFRE) fungi, remains largely unknown.</li> <li>While both AM fungi and MFRE transfer nutrients, including nitrogen, from inorganic and organic sources to host plants, their combined effects on co-colonised plants have only been investigated in liverworts. Here, we compare the morphology and physiology of the grass <em>Holcus lanatus</em> grown with an AM fungal community versus a more diverse symbiotic fungal community containing both AM fungi and MFRE. </li> <li> <em>Holcus lanatus</em> plants were grown in the presence of either a diverse MFRE+AM fungi soil inoculum or a multi-species AM fungal inoculum. Plant traits associated with growth were quantified, along with fungal transfer of <sup>15</sup>N tracer to plants from a variety of sources (ammonium chloride, alanine, glycine, algal necromass). </li> <li> <em>Holcus lanatus</em> grown with the AM fungal community had greater root and shoot growth during early development and prior to the addition of <sup>15</sup>N-labelled sources, compared to plants grown with the more diverse symbiotic fungal community. When nitrogen sources were made available to the fungal symbionts in the pot microcosms, plants growing with the MFRE+AM fungi soil inoculum had a faster growth rate than plants growing with the AM fungal community. At harvest, <em>H. lanatus</em> grown with the AM fungal community had a larger biomass and there were no differences in <sup>15</sup>N tracer assimilation in plants across the two fungal community treatments.</li> <li>Our results demonstrate that the diversity of fungal inocula in conjunction with soil nutrient availability determines the benefits derived by plants from diverse fungal symbionts. Our research contributes to understanding host plant outcomes in diverse multi-symbiont scenarios.</li> </ol>
Data from: A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts
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Data from: Comparative phylogeography, genetic differentiation, and contrasting reproductive modes in three fungal symbionts of a multipartite bark beetle symbiosis
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Data from: A heritable symbiont and host-associated factors shape fungal endophyte communities across spatial scales
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Data from: Fungal and algal lichen symbionts show different transcriptional expression patterns in two climate zones
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Data from: Road disturbance shifts root fungal symbiont types and reduces the connectivity of plant-fungal co-occurrence networks in mountains
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Fungal symbiont diversity drives growth of Holcus lanatus depending on soil nutrient availability
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FIGURE 2 in The granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera Curculionidae, Scolytinae), and its fungal symbiont found in South Africa
FIGURE 2. Phylogenetic and morphological identification of Ambrosiella roeperi. A Phylogram produced from maximum likelihood analysis using RAxML for the ITS region of isolates of A. roeperi. Isolates obtained and sequenced in this study are highlighted in bold. Nodes with bootstrap support higher than 75 are indicated. B Culture morphology of a two-week-old isolate grown on MEA (top left) and microscopic characters of A. roeperi. Scale bars: 50µm.
FIGURE 1 in The granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera Curculionidae, Scolytinae), and its fungal symbiont found in South Africa
FIGURE 1. Phylogenetic and morphological identification of Xylosandrus crassiusculus. A Phylogram produced from maximum likelihood analysis using RAxML for the COI region of specimens of X. crassiusculus. Specimens sequenced in this study are highlighted in bold. Nodes with bootstrap support higher than 75 are indicated. B Lateral and dorsal view of a X. crassiusculus female. Scale bars: 2 mm.
Data from: Saprotrophic and ectomycorrhizal fungal sporocarp stoichiometry (C : N : P) across temperate rainforests as evidence of shared nutrient constraints among symbionts
Summary: Quantifying nutritional dynamics of free-living saprotrophs and symbiotic ectomycorrhizal fungi (EMF) in the field is challenging, but the stoichiometry of fruiting bodies (sporocarps) may be an effective methodology for this purpose. Carbon (C), nitrogen (N), and phosphorus (P) concentrations of soils, foliage and 146 sporocarp collections were analyzed from 14 Pseudotsuga menziesii var. menziesii stands across a podzolization gradient on Vancouver Island (Canada). N and P concentrations were considerably higher in saprotrophic fungi. Fungal N% increased with soil N content at a greater rate for saprotrophs than EMF, while fungal P% of saprotrophs was more constrained. Fungal N:P was more responsive to soil N:P for EMF (homeostatic regulation coefficient 'H' =2.9) than saprotrophs (H= 5.9), while N:P of EMF and host tree foliage scaled almost identically. Results underscore the role of EMF as nutrient conduits, supporting host trees, whereas saprotrophs maintain a greater degree of nutritional homeostasis. Site nutrient constraints were shared in equal measure between EMF and host trees, particularly for P, suggesting neither partner benefits from enhanced nutrition at the expense of the other. Sporocarp stoichiometry provides new insights into mycorrhizal relationships and illustrates pervasive P deficiencies across temperate rainforests of the Pacific Northwest.
Data from: Genetic and genomic evidence of niche partitioning and adaptive radiation in mountain pine beetle fungal symbionts
Bark beetles form multipartite symbiotic associations with blue stain fungi (Ophiostomatales, Ascomycota). These fungal symbionts play an important role during the beetle's life cycle by providing nutritional supplementation, overcoming tree defences and modifying host tissues to favour brood development. The maintenance of stable multipartite symbioses with seemingly less competitive symbionts in similar habitats is of fundamental interest to ecology and evolution. We tested the hypothesis that the coexistence of three fungal species associated with the mountain pine beetle is the result of niche partitioning and adaptive radiation using SNP genotyping coupled with genotype–environment association analysis and phenotypic characterization of growth rate under different temperatures. We found that genetic variation and population structure within each species is best explained by distinct spatial and environmental variables. We observed both common (temperature seasonality and the host species) and distinct (drought, cold stress, precipitation) environmental and spatial factors that shaped the genomes of these fungi resulting in contrasting outcomes. Phenotypic intraspecific variations in Grosmannia clavigera and Leptographium longiclavatum, together with high heritability, suggest potential for adaptive selection in these species. By contrast, Ophiostoma montium displayed narrower intraspecific variation but greater tolerance to extreme high temperatures. Our study highlights unique phenotypic and genotypic characteristics in these symbionts that are consistent with our hypothesis. By maintaining this multipartite relationship, the bark beetles have a greater likelihood of obtaining the benefits afforded by the fungi and reduce the risk of being left aposymbiotic. Complementarity among species could facilitate colonization of new habitats and survival under adverse conditions.
Data from: Fungal symbionts as manipulators of plant reproductive biology
Symbioses have shaped the evolution of life, most notably through the fixation of heritable symbionts into organelles. The inheritance of symbionts promotes mutualism and fixation by coupling partner fitness. However, conflicts arise if symbionts are transmitted through only one sex and can shift host resources toward the sex through which they propagate. Such reproductive manipulators have been documented in animals with separate sexes but not in other phyla or sexual systems. Here we investigated whether the investment in male relative to female reproduction differed between hermaphroditic host plants with versus without a maternally inherited fungal symbiont. Plants with the fungus produced more seeds and less pollen than plants lacking the fungus, resulting in an ∼40% shift in functional gender and a switch from male-biased to female-biased sex allocation. Given the ubiquity of endophytes in plants, reproductive manipulators of hermaphrodites may be widespread in nature.
Data from: Loss of fungal symbionts at the arid limit of the distribution range in a native Patagonian grass – resource ecophysiological relations
<p>1. Crucial to our understanding of plant ecology is the consideration of the eco-physiological responses and constraints of plant-fungal symbioses throughout the native distribution range of their host.</p> <p>2. We examined key eco-physiological roles of two co-occurring fungal symbionts [Epichloë endophytes and arbuscular mycorrhizal fungi (AMF)] in the endemic grass Hordeum comosum across a wide bioclimatic gradient and contrasting grazing severity. We sampled H. comosum plants along four humid-to-arid transects in Patagonia, Argentina, covering its entire distribution range and determined Epichloë presence, AMF root colonization, nitrogen and phosphorus concentration, intrinsic water use-efficiency (iWUE, the ratio of photosynthesis to stomatal conductance) and 18O-enrichment of cellulose in shoots.</p> <p>3. Root colonization by AMF increased with Epichloë-presence. All plants hosted Epichloë in the humid range of the gradient, but symbioses occurrence decreased towards arid sites which also displayed severe grazing symptoms at site level.</p> <p>4. Symbiosis with Epichloë correlated positively with shoot nitrogen concentration in the centre of the distribution range, and with shoot phosphorus concentration across the entire distribution range.</p> <p>5. The site-level relationship of AMF colonization with 18O-enrichment and iWUE suggested that mycorrhiza boosted stomatal conductance in humid environments but curbed it in arid environments.</p> <p>6. While the interpretation of interactions and potential causalities from observational studies should be done with caution, this study demonstrates distinct correlations between plant-fungal symbiont associations and key resource parameters (phosphorus, nitrogen, and iWUE vs 18O-enrichment). Such correlations may suggest particular functional roles for these symbionts in the ecology of their host plant.</p>
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