Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
67
datasets available to search
ShareScore release 0.9.0
Dataset results
67 results for “gametocytes”
FIG. 6. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 6. — H. ptyodactyli (cont.); A, macrogametocyte with more dilute ribosomal contents and nucleus with a conspicuous nucleolus; B, large concentrically structured mitochondria; C-G, Haemoproteus tarentolae from Tarentola mauritanica; C-D, whole view of microgametocyte containing osmiophilic bodies, with folded attenuated ends; E, gametocyte with dense ribosomal contents; F, part of microgametocyte showing lobate nucleus, phagosomal residue inclusions and mitochondria; G, extremely lobate gametocyte. Abbreviations: e, vacuole with phagosomal residues; er, endoplasmic reticulum and connected cisternae; fe, empty food vacuole; m, mitochondria; n, nucleus; nl, nucleolemma; nu, nucleolus; o, osmiophilic bodies; w, electron-lucent space. Scale bars: A-C, E-G, 1 µm; D, 0.5 µm.
FIG. 4. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 4. — H. oedurae (cont.); A, whole view of H. oedurae microgametocyte with three Golgi-like organelles and a few osmiophilic bodies; B-E, Haemoproteus ptyodactyli from Ptyodactylus hasselquistii; B, whole view of an apparently macrogametocyte with dense ribosomal contents; note small osmiophilic bodies; C, juvenile gametocyte with sub-pellicular arrays of microtubules; D, juvenile gametocytes exhibiting wall details (M, arrows) and cross sections of sub-pellicular microtubules; E, gametocyte showing reforming M3 (possibly also M2) (Mr) and large food vacuole. Abbreviations: e, vacuole with phagosomal residues; e', inclusion filled with electron-dense globules; fi, food vacuole; g, Golgi; M1, 2 and 3, parasite's outer, median and inner boundary membranes; m, mitochondria; n, nucleus; nu, nucleolus; o, osmiophilic bodies; t, tubular structures; tt, true microtubules; v, vacuole. Scale bars: A-C, E, 0.5 µm; D, 0.25 µm.
FIG. 1 in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 1. — Haemoproteus edomensis in erythrocytes of Agama stellio; A, macrogametocyte with dense ribosomal contents, showing large inclusion with phagosomal residues, mitochondria and a vacuole; B, macrogametocyte (with dense ribosomal contents) showing the compact nucleus, two adjacent golgi-like tubular structures, inclusions, one containing membranous vesicular residue (e) and the other electron dense deposit (ee); C, macrogametocyte with tail-like ending (arrows), containing a compact nucleus with large nucleolus, an inclusion with phagosomal residue, mitochondria and "trapezoid" organelle; D, higher magnification view for details of the boundary membranes; E, detailed view of boundary membrane accompanied beneath by er extension; F, higher magnified view of a macrogametocyte, with compact nucleus, containing a conspicuous nucleolus, next to which can be seen an aggregate of granular matrix (arrow head); the cytoplasm contains bundles of er, a "trapezoid" and residue inclusions; G, unit membrane-bound food vacuole; H, detail from a juvenile gametocyte, showing a slit between the two lamellae of M1 and a heavy deposit between M1 and M2 (arrow); I, juvenile gametocyte showing a bundle of tubular structures (within a cisterna) and an electron-dense M3 membrane. Abbreviations: e, vacuole with phagosomal residues; ee, inclusion with electron-dense deposit; e', inclusion filled with electron-dense material; er, endoplasmic reticulum and connected cisternae; fi, food vacuole; g, Golgi; M1, 2 and 3 parasite's outer, median and inner boundary membranes; m, mitochondria; n, nucleus; t, tubular structures; tr, "trapezoid organelle"; v, vacuole. Scale bars: A-C, F-H, 1 µm; D, 2.5 µm; E, I, 0.5 µm.
FIG. 2. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 2. — H. edomensis (cont.); A, enlarged view (macrogametocyte?) showing mitochondria, some with electron-dense deposit (open arrow), Golgi-like organelle, vacuoles with phagosomal residue, trapezoid body and inclusions of unknown nature; B, apparently juvenile stage with nucleus containing two (split) nucleoli, some endoplasmic reticulum is filled with electron-dense material (larger arrow), and M3 is also visible (smaller arrows), tubular structures are enclosed within an endoplasmic reticulum cisterna; C, view of tubulies inside cisternae; D, macrogametocyte with dense ribosomal contents, a compact nucleus and a Golgi-like organelle; E-F, Haemoproteus mackerrasi; E, H. mackerrasi gametocyte inside an erythrocyte of Heteronota binoei; F, H. mackerrasi in gut contents of Culex fatigans induced to engorge Heteronota binoei. Note loose, undulating lamina remaining of M1 membrane (arrows). Abbreviations: cy, cytostome; e, vacuole with phagosomal residues; ec, inclusion with crystalline deposit (hemozoin); g, Golgi; m, mitochondria; n, nucleus; nu, nucleolus; o, osmiophilic bodies; rer, rough endoplasmic reticulum; t, tubular structures; tr, "trapezoid organelle"; v, vacuole. Scale bars: 1 µm.
FIG. 5. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 5. — H. ptyodactyli (cont.); A, whole view of a macrogametocyte; note an inclusion of unknown entity, i, and sub-pellicular vacuoles, vi; B, juvenile gametocyte with conspicuous cytostome connected to food vacuole, with adjacent empty food vacuole (fe); C, folded microgametocyte (Mi) with lobated nucleus and numerous osmiophilic bodies, note its lower ribosomal content in comparison to the dense ribosomal content in the cytoplasm of the adjacent macrogametocyte (Ma); D, sector of a microgametocyte showing osmiophilic bodies connected by a ductule with the cell boundary zone (empty arrow - inclusion of unknown entity); E, cross section of a microgametocyte with numerous osmiophilic bodies. Abbreviations: cy, cytostome; e, vacuole with phagosomal residues; ec, inclusion with crystalline deposit (hemozoin); er, endoplasmic reticulum and connected cisternae; fi, food vacuole; g, Golgi; m, mitochondria; n, nucleus; o, osmiophilic bodies; tt, true microtubules; v, vacuole. Scale bars: A, B, D, 0.5 µm; C, 2 µm; E, 1 µm.
FIG. 3. — A-B, H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 3. — A-B, H. mackerrasi (cont.); A, enlarged view of intraerythrocytic H. mackerrasi; B, enlarged detail to show wall membranes of H. mackerrasi infecting an intrahepatic proerythrocyte; C-F; Haemoproteus oedurae, apparently microgametocyte stages, from Oedura castelnaui; C, general view; D, microgametocyte with a very large mitochondrion and a conspicuous Golgi-like organelle; E, anterior end, showing oblong osmiophilic bodies; F, detailed view of Golgi-like organelle. Abbreviations: ec, inclusion with crystalline deposit (hemozoin); er, endoplasmic reticulum and connected cisternae; g, Golgi; M1, 2 and 3, parasites' outer, median and inner boundary membranes; m, mitochondrion; n, nucleus; o, osmiophilic body. Scale bars: 0.5 µm.
FIG. 8. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 8. — H. tarentolae (cont.); A, juvenile gametocyte; B, another microgametocyte with the central electron-lucent space. Abbreviations: e, vacuole with phagosomal residues; fi, food vacuole; n, nucleus; o, osmiophilic bodies; w, electron-lucent space. Scale bars: 1 µm.
FIG. 7. — H in Fine structure of gametocytes in five species of Haemoproteus (Haemosporidia) from geckos and agamid lizards
FIG. 7. — H. tarentolae (cont.), a microgametocyte with central, large, electron-lucent space. Abbreviations: n, nucleus; o, osmiophilic bodies; w, electron-lucent space. Scale bar: 1 µm.
Data from: Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes
Open the record for dataset details and reuse information.
Plasmodium falciparum gametocyte density and infectivity in peripheral blood and skin tissue of naturally infected parasite carriers in Burkina Faso
<p>Background </p><p>Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a blood meal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested.</p> Methods <p>In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in finger-prick and venous blood samples, skin biopsy samples, and in of mosquitoes that fed on venous blood or directly on skin. Gametocytes were visualized in skin tissue with confocal microscopy.</p> Results <p>Although more mosquitoes became infected when feeding directly on skin then when feeding on venous blood (odds ratio, 2.01; 95% confidence interval, 1.21–3.33; P = .007), concentrations of gametocytes were not higher in the subdermal skin vasculature than in other blood compartments; only sparse gametocytes were observed in skin tissue.</p> Discussion <p>Our data strongly suggest that there is no significant skin sequestration of P. falciparum gametocytes. Gametocyte densities in peripheral blood are thus informative for predicting onward transmission potential to mosquitoes and can be used to target and monitor malaria elimination initiatives.</p> <p></p>
Data from: Predicting mosquito infection from Plasmodium falciparum gametocyte density and estimating the reservoir of infection
Transmission reduction is a key component of global efforts to control and eliminate malaria; yet, it is unclear how the density of transmission stages (gametocytes) influences infection (proportion of mosquitoes infected). Human to mosquito transmission was assessed using 171 direct mosquito feeding assays conducted in Burkina Faso and Kenya. Plasmodium falciparum infects Anopheles gambiae efficiently at low densities (4% mosquitoes at 1/μl blood), although substantially more (>200/μl) are required to increase infection further. In a site in Burkina Faso, children harbour more gametocytes than adults though the non-linear relationship between gametocyte density and mosquito infection means that (per person) they only contribute slightly more to transmission. This method can be used to determine the reservoir of infection in different endemic settings. Interventions reducing gametocyte density need to be highly effective in order to halt human–mosquito transmission, although their use can be optimised by targeting those contributing the most to transmission.
Data from: Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection.
Submicroscopic Plasmodium falciparum gametocytemia (<5,000 gametocytes/mL) is common and may result in mosquito infection. We assessed the relation between gametocyte density and mosquito infection under experimental and field conditions using real-time quantitative nucleic acid sequence-based amplification (QT-NASBA) for gametocyte quantification. Serial dilutions of NF54 P. falciparum gametocytes showed a positive association between gametocyte density and the proportion of infected mosquitoes (beta=6.1; 95% confidence interval [CI], 2.7-9.6; P=0.001). Successful infection became unlikely below an estimated density of 250-300 gametocytes/mL. In the field, blood samples of 100 naturally infected children showed a positive association between gametocyte density and oocyst counts in mosquitoes (beta=0.38; 95% CI, 0.14-0.61; P=0.002). The relative contribution to malaria transmission was similar for carriers with submicroscopic and microscopic gametocytemia. Our results show that transmission occurs efficiently at submicroscopic gametocyte densities and that carriers harboring submicroscopic gametocytemia constitute a considerable proportion of the human infectious reservoir.
Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission.
Background: Man to mosquito transmission of malaria depends on the presence of the sexual stage parasites, gametocytes, that often circulate at low densities. Gametocyte densities below the microscopical threshold of detection may be sufficient to infect mosquitoes but the importance of submicroscopical gametocyte carriage in different transmission settings is unknown. Methodology/Principal Findings: Membrane feeding experiments were carried out on 80 children below 14 years of age at the end of the wet season in an area of seasonal malaria transmission in Burkina Faso. Gametocytes were quantified by microscopy and by Pfs25-based quantitative nucleic acid sequence-based amplification assay (QT-NASBA). The children's infectiousness was determined by membrane feeding experiments in which a venous blood sample was offered to locally reared Anopheles mosquitoes. Gametocytes were detected in 30.0% (24/80) of the children by microscopy compared to 91.6% (65/71) by QT-NASBA (p<0.001). We observed a strong association between QT-NASBA gametocyte density and infection rates (p = 0.007). Children with microscopically detectable gametocytes were more likely to be infectious (68.2% compared to 31.7% of carriers of submicroscopical gametocytes, p = 0.001), and on average infected more mosquitoes (13.2% compared to 2.3%, p<0.001). However, because of the high prevalence of submicroscopical gametocyte carriage in the study population, carriers of sub-microscopical gametocytes were responsible for 24.2% of the malaria transmission in this population. Conclusions/Significance: Submicroscopical gametocyte carriage is common in an area of seasonal transmission in Burkina Faso and contributes substantially to the human infectious reservoir. Submicroscopical gametocyte carriage should therefore be considered when implementing interventions that aim to reduce malaria transmission.
Efficacy and Safety of a Single Low-dose Primaquine for the Clearance of Gametocytes
ClinicalTrials.gov study NCT02090036. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Low Dose Primaquine for Clearance of Gametocytes
ClinicalTrials.gov study NCT01935882. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Data from: Predicting mosquito infection from Plasmodium falciparum gametocyte density and estimating the reservoir of infection
Open the record for dataset details and reuse information.
Data from: Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection.
Open the record for dataset details and reuse information.
Plasmodium falciparum gametocyte density and infectivity in peripheral blood and skin tissue of naturally infected parasite carriers in Burkina Faso
Open the record for dataset details and reuse information.
Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission.
Open the record for dataset details and reuse information.
Data from: Higher gametocyte production and mosquito infectivity in chronic compared to incident Plasmodium falciparum infections
Open the record for dataset details and reuse information.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.