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48 results for “gamma irradiation”

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zenodo40/100

Fig. 1 in Evidence of Stress Recovery in Free-Living Ciliate Colpoda cucullus: The Repair Capability of Resting Cysts to Damage Caused by Gamma Irradiation

Fig. 1. Excystment assay of Colpoda wet cysts (A) and dry cysts (B). 'Non-irradiated' indicates non-irradiated cysts; 'irradiated' indicates cysts irradiated at 4000 Gy, and 'irradiated-incubated' indicates cysts irradiated at 4000 Gy and incubated for 12 hours before the induction of excystment. Time indicates the number of hours after the induction of excystment. Columns and attached bars correspond to the means and standard errors, respectively, of six measurements. Asterisks and double asterisks represent significant differences at p <0.05 and p <0.01 (Mann-Whitney U test), respectively.

opencc-by-4.0Dec 2019View details →
zenodo40/100

Composite of graphene and silver nanoparticles obtained by low-dose gamma irradiation

<p>Content:</p> <p>TEM.zip - TEM images of composites, file type .tif and .dm3</p> <p>VINCA_FTIR.zip - FTIR data of graphene oxide and exfoliated graphene, file type .csv</p> <p>VINCA_UV-Vis.zip - FTIR data of graphene oxide and exfoliated graphene composites, file type .csv</p> <p>IEMN_VNA_Graphene-AgNP - EMI shielding measurement of graphene oxide and exfoliated graphene composites, file type .xlsx</p> <p>VINCA_CA_graphene-AgNP.jpg - contact angle measurement of graphene oxide and exfoliated graphene composites</p> <p>VINCA_532nmLASER.zip - temperature elevation measurements of graphene oxide and exfoliated graphene composites, file type .csv</p> <p>FTPO_TGA_Graphene-AgNP.zip - TGA data of graphene oxide and exfoliated graphene composites, file type .txt</p> <p>&nbsp;</p> <p>&nbsp;</p>

opencc-by-4.0Apr 2024View details →
zenodo40/100

Fig. 2 in Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Fig. 2. Analysis of proteins (Left panel) and protein carbonylation by ECL (Right panel) from non-irradiated and 4000 Gy irradiated cells. The samples in the lanes were from non-irradiated cells (NonIR), 4000 Gy irradiated cells (IR), and cells incubated for 12 h after 4000 Gy irradiation (IR incubated). The protein bands and ECL signals were measured and are shown in parentheses for each lane relative to the Non-IR sample.

opencc-by-4.0Dec 2020View details →
zenodo40/100

Fig. 4 in Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Fig. 4. Relative viability of Colpoda vegetative cells, wet cysts, and dry cysts after gamma radiation doses of 0 (non-irradiated), 500, 1000, 2000, 3000, and 4000 Gy. The column heights and attached bars are the means and standard errors, respectively, of six measurements at each dose. Double asterisks indicate a significant difference at p &lt;0.01 (Mann-Whitney U test).

opencc-by-4.0Dec 2020View details →
zenodo40/100

Fig. 3 in Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Fig. 3. Excystment of Colpoda dry cysts, after gamma irradiation at 0 (non-irradiated), 500, 1000, 2000, 3000, and 4000 Gy, as a function of time after induction of excystment. The points and bars mark the means and standard errors, respectively, of six measurements at each dose. The excystment mean ± SE at 3, 6, 24, and 96 h after the induction of excystment is shown in (a), (b), (c), and (d), respectively. The column heights and attached bars in (a) to (d) are the means and standard errors, respectively, of six measurements at each dose. Asterisks and double asterisks indicate a significant difference at p &lt;0.05 and p &lt;0.01, respectively (Mann-Whitney U test).

opencc-by-4.0Dec 2020View details →
zenodo40/100

Fig. 1 in Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Fig. 1. Excystment of Colpoda wet cysts, after gamma irradiation at 0 (non-irradiated), 500, 1000, 2000, 3000, and 4000 Gy, as a function of time after the induction of excystment. The points and bars mark the means and standard errors, respectively, of six measurements at each dose. The excystment mean ± SE at 3, 6, 9, and 36 h after induction of excystment is shown in (a), (b), (c), and (d), respectively. The column heights and attached bars in (a) to (d) are the means and standard errors, respectively, of six measurements. Asterisks and double asterisks indicate a significant difference at p &lt;0.05 and p &lt;0.01, respectively (Mann-Whitney U test).

opencc-by-4.0Dec 2020View details →
zenodo40/100

Fig. 2 in Flight ability and dispersal of European grapevine moth gamma-irradiated males (Lepidoptera: Tortricidae)

Fig. 2. Schematic representation of the vineyard and the pheromone traps positions at the experimental plot where the marked male moths were released.

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig 1 in Flight ability and dispersal of European grapevine moth gamma-irradiated males (Lepidoptera: Tortricidae)

Fig 1. Schematic representation of the flight assessment cage used to measure the flight responses of Lobesia botrana males to calling females. In the female compartment, 2-day-old virgin females were confined inside a small cylindrical plastic mesh box with a 5% sucrose-wetted wick. Males irradiated either with 150 Gy or with 350 Gy and untreated males differentially marked with variously colored fluorescent powders were introduced into the male compartment. The number of males of each of the 3 kinds that flew through the open slit at the 45 cm height [the 2 lower openings (slits) were sealed] into the female compartment were recorded at 24, 48, 72 and 96 h. Air was drawn into the female compartment and exhausted from the male compartment.

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig. 2 in Development, reproduction and sexual competitiveness of Conopomorpha sinensis (Lepidoptera: Gracillariidae) gamma-irradiated as pupae and adults

Fig. 2. Mean (± SE) mortality of eggs oviposited by Conopomorpha sinensis females involved in 3 different crosses. The upper curve shows the percentage hatch of eggs from the cross, UF × TM in which males were γ-irradiated as mature pupae with doses ranging egg from 50 and 300 Gy. The middle curve shows the corresponding results from the cross, TF × UM, however females irradiated with 200–300 Gy did not oviposit any eggs. The lower curve shows the corresponding results when both parents were irradiated.

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig. 1 in Development, reproduction and sexual competitiveness of Conopomorpha sinensis (Lepidoptera: Gracillariidae) gamma-irradiated as pupae and adults

Fig. 1. Fecundity of P generation Conopomorpha sinensis adults that emerged from pupae irradiated at various γ-radiation doses ranging from 50 to 300 Gy. UF = non-irradiated females, TF = treated females, UM = non-irradiated males, TM = treated males. Different letters above bars indicate statistically significant differences within each combination (DMRT, P = 0.05).

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig. 2 in Influence of gamma-irradiation on flight ability and dispersal of Conopomorpha sinensis (Lepidoptera: Gracillariidae)

Fig. 2. Numbers of adult Conopomorpha sinensis males recaptured in traps deployed at various distances (m) from the release point in 2 release/recapture experiments in a litchi orchard of the South China Agricultural University, Guangzhou, China. The males were either non-irradiated or irradiated either with 150 or 200 Gy. A: First release, B: Second release.

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig. 1 in Influence of gamma-irradiation on flight ability and dispersal of Conopomorpha sinensis (Lepidoptera: Gracillariidae)

Fig. 1. Survival (days) of non-irradiated Conopomorpha sinensis males either dyed with 1 of 3 different fluorescent colors or undyed (control) in the laboratory. Each treatment involved 30 males.

opencc-by-4.0Jun 2016View details →
zenodo40/100

Fig. 3 in Influence of gamma-irradiation on flight ability and dispersal of Conopomorpha sinensis (Lepidoptera: Gracillariidae)

Fig. 3. Frequency distributions of the dispersal directions of adult Conopomorpha sinensis males in 2 release/recapture experiments in a litchi orchard of the South China Agricultural University, Guangzhou, China. The males were either non-irradiated or irradiated either with 150 or 200 Gy. A: First release, B: Second release.

opencc-by-4.0Jun 2016View details →
zenodo36/100

Confocal microscopy of gH2AX and 53BP1 DNA repair foci of cells exposed to gamma-irradiation, pt. 2/3

<p><strong>Summary</strong></p> <p>Dataset of confocal microscopy data of cells exposed to gamma-irradiation and immunostained with gH2AX and 53BP1.</p> <ul> <li><strong>Part 1/3: </strong>Head and neck primocultures immunostained with gH2AX/53BP1, Testing dataset. DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2564980">10.5281/zenodo.2564980</a></li> <li><strong>Part 2/3 (this dataset)</strong>: U-87 and NHDF Cells exposed to 1-4 Gy confocal microscopy data of head and neck tumor primocultures immunostained with gH2AX/53BP1. Testing dataset. DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2572450">10.5281/zenodo.2572450</a>.&nbsp;174 TIFFs</li> <li><strong>Part 3/3</strong>: training dataset for nuclei and gH2AX foci with ground truth annotation masks, head and neck primocultures (head and neck non-tumor and spinocellular tumor cells). DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2576241">10.5281/zenodo.2576241</a>.&nbsp;150 TIFFs for nuclei learning incl 150 png nuclei masks + 99 TIFFs for DNA repair foci training</li> <li><strong>Code</strong>: the code is available at&nbsp;<a href="https://github.com/tomasvicar/LearnFoci">https://github.com/tomasvicar/LearnFoci</a></li> </ul> <p><strong>Materials and methods</strong></p> <p><em>Dataset</em></p> <p>Following cells were used: (1) patient primocultures of patients with &nbsp;spinocellular head and neck tumors (histologically verified tumor and tumor-adjacent tissues), isolation protocol descibed in <em>Svobodova et al, 2017</em>, (2) primary glioblastoma cell line U-87 (ATCC HTB-14, LGC Standards, United Kingdom), (3) primary normal human dermal fibroblasts (NHDF, PromoCell, Hedelberg, Germany) isolated from the dermis of juvenile foreskin or adult skin.<br> The study was conducted in accord with the Helsinki Declaration of 1964 and all subsequent revisions thereof. It was approved by the ethical committee of St. Anne&rsquo;s Faculty Hospital, Brno. Primoculture cells were cultivated in Pen/Strep antibiotic solution (PAA Laboratories GmbH, Austria) in RPMI-1640 medium with 10% FBS (Biochrom, USA) at 37 &deg;C and 50% CO2 in humidified atmosphere up to 50% confluence. U-87 were grown in Eagle&#39;s MEM with 10% FBS.</p> <p><em>Gamma irradiation</em></p> <p>The cells were irradiated at the Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic in a following schemes: (a) patient-derived primoculture was irradiated with a single dose of 2 Gy (D = 1 Gy/min) of gamma-rays (60Co, Chisostat, Chirana, CR) , (b) U-87 and NHDF cells were irradiated with doses 1, 2, and 4 Gy (D = 1 Gy/min). Cells were irradiated in RPMI 1640 medium (37 &deg;C, normal atmosphere). Confocal microscopy of gammaH2AX and 53BP1 foci immunodetection was consequently performed.</p> <p><em>Fluorescent staining </em></p> <p>DNA double strand breaks (DSBs) were quantified in different periods of time post-irradiation (30 min, 8h and 24h post irradiation) by means of $\gamma$H2AX and 53BP1 foci immunodetection combined with &nbsp;confocal microscopy. For details see \cite{falk2007chromatin}.</p> <p><em>Confocal microscopy</em></p> <p>The microscopy of samples was performed at the Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic. Leica DM RXA microscope (equipped with DMSTC motorized stage, Piezzo z-movement, MicroMax CCD camera, CSU-10 confocal unit and 488, 562, and 714 nm laser diodes with AOTF) was used for acquiring detailed cell images (100&times; oil immersion Plan Fluotar lens, NA 1.3). Total 50 Z slices was captured with Z step size 0.3 &mu;m.</p> <p><strong>File description</strong></p> <p>all files are&nbsp;compressed hyperstack tiffs (50 Z slices and 3 fluorescent channels, XYCZ order), 100x magnification</p> <ul> <li>Confocal_NHDF_cells_IR_1-4Gy.zip:&nbsp;Human fibroblast NHDF cell line, exposed to gamma irradiation doses 1, 2, and 4 Gy, 64 TIFFs</li> <li>Confocal_U-87_cells_IR_1-4Gy.zip: Human glioblastoma U-87 cell line, exposed to gamma irradiation doses 1, 2, and 4 Gy, 108 TIFFs</li> </ul>

opencc-by-4.0Feb 2019View details →
zenodo36/100

Confocal microscopy of gH2AX and 53BP1 DNA repair foci of cells exposed to gamma-irradiation, pt. 1/3

<p><strong>Summary</strong></p> <p>Dataset of confocal microscopy data of cells exposed to gamma-irradiation and immunostained with gH2AX and 53BP1.</p> <ul> <li><strong>Part 1/3 (this dataset): </strong>Head and neck primocultures immunostained with gH2AX/53BP1, Testing dataset. DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2564980">10.5281/zenodo.2564980</a></li> <li><strong>Part 2/3</strong>: U-87 and NHDF Cells exposed to 1-4 Gy confocal microscopy data of head and neck tumor primocultures immunostained with gH2AX/53BP1. Testing dataset. DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2572450">10.5281/zenodo.2572450</a>.&nbsp;174 TIFFs</li> <li><strong>Part 3/3</strong>: training dataset for nuclei and gH2AX foci with ground truth annotation masks, head and neck primocultures (head and neck non-tumor and spinocellular tumor cells). DOI&nbsp;<a href="https://doi.org/10.5281/zenodo.2576241">10.5281/zenodo.2576241</a>.&nbsp;150 TIFFs for nuclei learning incl 150 png nuclei masks + 99 TIFFs for DNA repair foci training</li> <li><strong>Code</strong>: the code is available at&nbsp;<a href="https://github.com/tomasvicar/LearnFoci">https://github.com/tomasvicar/LearnFoci</a></li> </ul> <p><strong>Materials and methods</strong></p> <p><em>Dataset</em></p> <p>Following cells were used: (1) patient primocultures of patients with &nbsp;spinocellular head and neck tumors (histologically verified tumor and tumor-adjacent tissues), isolation protocol descibed in <em>Svobodova et al, 2017</em>, (2) primary glioblastoma cell line U-87 (ATCC HTB-14, LGC Standards, United Kingdom), (3) primary normal human dermal fibroblasts (NHDF, PromoCell, Hedelberg, Germany) isolated from the dermis of juvenile foreskin or adult skin.<br> The study was conducted in accord with the Helsinki Declaration of 1964 and all subsequent revisions thereof. It was approved by the ethical committee of St. Anne&rsquo;s Faculty Hospital, Brno. Primoculture cells were cultivated in Pen/Strep antibiotic solution (PAA Laboratories GmbH, Austria) in RPMI-1640 medium with 10% FBS (Biochrom, USA) at 37 &deg;C and 50% CO2 in humidified atmosphere up to 50% confluence. U-87 were grown in Eagle&#39;s MEM with 10% FBS.</p> <p><em>Gamma irradiation</em></p> <p>The cells were irradiated at the Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic in a following schemes: (a) patient-derived primoculture was irradiated with a single dose of 2 Gy (D = 1 Gy/min) of gamma-rays (60Co, Chisostat, Chirana, CR) , (b) U-87 and NHDF cells were irradiated with doses 1, 2, and 4 Gy (D = 1 Gy/min). Cells were irradiated in RPMI 1640 medium (37 &deg;C, normal atmosphere). Confocal microscopy of gammaH2AX and 53BP1 foci immunodetection was consequently performed.</p> <p><em>Fluorescent staining </em></p> <p>DNA double strand breaks (DSBs) were quantified in different periods of time post-irradiation (30 min, 8h and 24h post irradiation) by means of $\gamma$H2AX and 53BP1 foci immunodetection combined with &nbsp;confocal microscopy. For details see \cite{falk2007chromatin}.</p> <p><em>Confocal microscopy</em></p> <p>The microscopy of samples was performed at the Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic. Leica DM RXA microscope (equipped with DMSTC motorized stage, Piezzo z-movement, MicroMax CCD camera, CSU-10 confocal unit and 488, 562, and 714 nm laser diodes with AOTF) was used for acquiring detailed cell images (100&times; oil immersion Plan Fluotar lens, NA 1.3). Total 50 Z slices was captured with Z step size 0.3 &mu;m.</p> <p><strong>File description</strong></p> <p>all files are&nbsp;compressed hyperstack tiffs (50 Z slices and 3 fluorescent channels, XYCZ order), 100x magnification</p> <ul> <li>Confocal_HN_tumor_primocultures.zip:&nbsp;dataset of patient-derived primocultures (gamma-irradiated with 2 Gy and controls), for description see file below, 100 FOVs,</li> <li>Confocal_HN_tumor_primocultures_description.xlsx: description of confocal dataset files and patient tumor characteristics</li> <li>Confocal_HN_tumor_primocultures_train_nuclei.zip: annotated training subset 1 of dataset of patient-derived primocultures (gamma-irradiated with 2 Gy and controls) used for nuclei segmentation training, 150 FOVs, includes TIFF and manually annotated binary mask for nuclei (data_###.tif and respective&nbsp;mask_###.png)</li> <li>Confocal_HN_tumor_primocultures_train_nuclei_description.xlsx: description of above-mentioned files (tissue type, TNM stage, grade, time post irradiation)</li> </ul>

opencc-by-4.0Feb 2019View details →
zenodo32/100

CTLA-4 and PD-1 expressed in EMT6 mouse mammary carcinoma cell line acquired radio-resistance to gamma-ray irradiation.

<p>Supplementary File of manuscript entitled&nbsp;CTLA-4 and PD-1 expressed in EMT6 mouse mammary carcinoma cell line acquired radio-resistance to gamma-ray irradiation.</p>

opencc-by-4.0Jan 2022View details →
zenodo32/100

Effects of Gamma Irradiation on Soil Samples

<p>Linear and mass attenuation coefficients are widely used in the study of gamma rays, calculation of photon penetration and energy deposition in biological shielding, in industry, agriculture and other materials. There are various reports on the measurements of these quantities by scientists.</p> <p>Singh et.al (1991) measured energy absorption coefficients for gamma rays in different soil samples by applying Bradley et. al .(1989) technique. Jahagirdar (1992) determined narrow beam attenuation coefficients of 320 keV photons, emitted from 51Cl radioisotope in various high Z materials such as (CH3COO)2.Pb.3H2O; Pb(NO3); Na2WO4.2H2O by using NaI (Tl) spectrometer system coupled to 1 k multichannel analyzer and results are compared with tabulations of Hubbel (1992). The self absorption factors were determined by Yu Cagin et.al.(1990).</p> <p>So far, evaluation of both types of coefficient (attenuation and energy absorption) for gamma rays has been performed for materials in solid and crystal forms. Mixture rule for theoretical calculation of absorption coefficients developed for elements by Hubbell (1982).</p> <p>Dr. M.T. Teli and Dr. L. M. Chaudhari (1994) developed mixture rule for liquid and studied the linear absorption coefficient of various gamma radiation by dilute solutions of various salts such as sodium chloride, potassium chloride, ammonium chloride and aluminium chloride, etc. at various concentrations by using gamma energies.</p> <p>In view of the importance of the study of gamma attenuation properties of materials and its various applications in science, technology, agriculture and human health, we have embarked on study of the absorption properties of soil samples containing various Chemical and Physical properties composed of various elements.</p> <p>By using the previous techniques, we explore the validity of the expected exponential absorption law for gamma radiation in soil samples collected from different areas of Nanded, Latur, Osmanabad District from Maharashtra and Bidar District from Karnataka of India. We also studied the behavior of soil density with gamma attenuation coefficient at various energies.</p>

opencc-by-4.0Apr 2023View details →
ClinicalTrials.gov32/100

Uses of Gamma Irradiated Amniotic Membrane as an Alternative Method in Psoriasis Treatment

ClinicalTrials.gov study NCT03440541. IPD Sharing: Not stated. Countries: 1. Publications: 14.

restrictedIPD-UNDECIDEDFeb 2026View details →
nasa28/100

Transcription profiling of atm mutant adm mutant and wild type whole plants and roots of Arabidopsis after gamma ray irradiation in a time series

Whole seedlings of wild type (4d) and atm mutants (4d) have been analyzed after a gamma ray irradiation of 0.75h 1.5h 3h & 5h (time course). Roots of wt (4d) atm (3d) and atr (4d) mutants have been analyzed after a 1h irradiation. Ataxia Telangiectasia Mutated (ATM) encodes a large protein with a phosphatidylinositol 3-kinase (PI3K)-like domain at the C terminus (reviewed by Rotman and Shiloh 1998). PI3K-related proteins make up a large family of Ser-Thr protein kinases numerous members of which are involved in the regulation of cell cycle progression responses to DNA damage and the maintenance of genomic stability (Hoekstra 1997). AtATM plays an essential role in meiosis and in the somatic response to DNA damage in plants similar to the function of ATM in mammals and other eukaryotes. Ataxia telangiectasia-mutated and Rad3-related (ATR) plays a central role in cell-cycle regulation transmitting DNA damage signals to downstream effectors of cell-cycle progression.

restrictedus-pdApr 2025View details →
geo24/100

Gene expression profile of peripheral blood leukocytes of 60Co gamma-ray accidently-irradiated persons

GEO Series GSE34131. Homo sapiens. 4 samples. Type: Expression profiling by array.

openGEO-OpenNov 2012View details →

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