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79 results for “genetic crosses”
Data from: Asymmetry in cross-sex cross-trait genetic covariances and the evolvability of sexual dimorphism
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Data from: Cross ocean-basin population genetic dynamics in a pelagic top predator of high conservation concern, the oceanic whitetip shark, Carcharhinus longimanus
<p>The oceanic whitetip shark, <em>Carcharhinus longimanus</em>, is a Critically Endangered, circumtropical, and highly migratory, pelagic shark. Yet, little information exists on its population genetic dynamics to guide conservation management practice. We present a first worldwide, mitochondrial and nuclear DNA assessment of the population genetic status of this imperiled species based on sequences of the complete mitochondrial control region (n = 173) and partial ND4 gene (n = 172), and genotypes from 12 nuclear microsatellites (n = 164). Statistically significant mitochondrial and nuclear DNA population genetic differentiation was detected across all marker datasets between Western Atlantic and Indo-Pacific oceanic whitetip sharks. Additionally, our data, combined with previously published, partial (701-base pairs) mitochondrial control region sequences from additional locations in the Atlantic and Indian Oceans, confirmed significant matrilineal population structure between the Western and Eastern Atlantic. The combined data also provisionally (i.e., with <em>F</em><sub>ST </sub>but not Φ<sub>ST</sub>) indicated differentiation between Western North and Central-South Atlantic sharks, pointing to the need for further assessment in this region. Matrilineal differentiation was also detected between Indian and Pacific Ocean sharks via pairwise analyses, albeit with the ND4 gene sequence only (Φ<sub>ST</sub> = 0.051; F<sub>ST</sub> = 0.092). Limited sampling in the Pacific leaves open questions about the connectivity dynamics in this large region. Despite the presence of geographic population genetic structure, the mitochondrial data showed no evidence of across ocean basin phylogeographic lineages. A provisional assessment of mitochondrial and nuclear genetic diversity indicated the oceanic whitetip shark's status falls in the middle to upper ranges compared to other shark species, potentially lending some optimism for the present adaptability and resiliency of this species if strong conservation measures are effectively implemented.</p>
Cross-correlations and symmetries in genetic sequences
<p>Data sets and supporting material used in the manuscript</p> <p>"The common origin of symmetry and structure in genetic sequences" by<br> G. Cristadoro , M. Degli Esposti , E. G. Altmann<br> https://arxiv.org/abs/1710.02348</p> <p>Output of calculations can be seen in the file:<br> Notebook.html</p> <p>In order to repeat calculations or explore different choices:</p> <ol> <li>Download all files to the same directory</li> <li>Uncompress the "data.tar.gz" file (e.g., using tar -xzvf data.tar.gz)</li> <li>Open the Notebook using Python 3.0 in Jupyter (www.jupyter.org)</li> </ol> <p> </p>
Result of LAVA-Knock: Genetic correlation via knockoffs removes confounding due to cross-trait assortative mating
<p>Results of application to GWAS summary statistics for 36 traits. 394,060 windows are used to perform LAVA-Bonf. 64,833 windows are used to conduct LAVA-Knock. At target FDR 0.1, LAVA-Knock detects 8,421 significant windows for 4,401 locus-phenotype pairs. LAVA-Bonf with threshold 0.05/394,060 identifies 10,802 windows for 6,260 locus-phenotype pairs.</p>
Crossing design shapes patterns of genetic variation in synthetic recombinant populations of Saccharomyces cerevisiae
<p>"Synthetic recombinant" populations have emerged as a useful tool for dissecting the genetics of complex traits. They can be used to derive inbred lines for fine QTL mapping, or the populations themselves can be sampled for experimental evolution. In latter application, investigators generally value maximizing genetic variation in constructed populations. This is because in evolution experiments initiated from such populations, adaptation is primarily fueled by standing genetic variation. Despite this reality, little has been done to systematically evaluate how different methods of constructing synthetic populations shape initial patterns of variation. Here we seek to address this issue by comparing outcomes in synthetic recombinant <i>Saccharomyces cerevisiae</i> populations<i> </i>created using one of two strategies: pairwise crossing of isogenic strains or simple mixing of strains in equal proportion. We also explore the impact of the varying the number of parental strains. We find that more genetic variation is initially present and maintained when population construction includes a round of pairwise crossing. As perhaps expected, we also observe that increasing the number of parental strains typically increases genetic diversity. In summary, we suggest that when constructing populations for use in evolution experiments, simply mixing founder strains in equal proportion may limit the adaptive potential.</p>
Cross-species transcriptomics uncovers genes underlying genetic accommodation of developmental plasticity in spadefoot toads
<p>That hardcoded genomes can manifest as plastic phenotypes responding to environmental perturbations is a fascinating feature of living organisms. How such developmental plasticity is regulated at the molecular level is beginning to be uncovered aided by the development of -omic techniques. Here, we compare the transcriptome-wide responses of two species of spadefoot toads with differing capacity for developmental acceleration of their larvae in the face of a shared environmental risk: pond drying. By comparing gene expression profiles over time and performing cross-species network analyses, we identified orthologues and functional gene pathways whose environmental sensitivity in expression have diverged between species. Genes related to lipid, cholesterol and steroid biosynthesis and metabolism make up most of a module of genes environmentally responsive in one species, but canalized in the other. The evolutionary changes in the regulation of the genes identified through these analyses may have been key in the genetic accommodation of developmental plasticity in this system.</p>
Immediate and long-term genetic consequences of linear transport infrastructure: Can fauna crossing mitigate its cost?
<p><span>The genetic consequences of the subdivision of populations are regarded as significant to long-term evolution, and research has shown that the scale and speed at which this is now occurring is critically reducing the adaptive potential of most species which inhabit human-impacted landscapes. Here, we provide a rare, and to our knowledge, the first analysis of this process while it is happening and demonstrate a method of evaluating the effect of mitigation measures such as fauna crossings. We did this by using an extensive genetic dataset collected from a koala population which was intensely monitored during the construction of linear transport infrastructure which resulted in the subdivision of their population. First, we found that both allelic richness and effective population size decreased through the process of population subdivision. Second, we predicted the extent to which genetic drift could impact genetic diversity over time and showed that after only 10 generations the resulting two subdivided populations could experience between 12–69% loss in genetic diversity. Lastly, using forward simulations we estimated that a minimum of 8 koalas would need to disperse from each side of the subdivision per generation to maintain genetic connectivity close to zero but that 16 koalas would ensure that both genetic connectivity and diversity remained unchanged. </span><span>These results have important consequences for the genetic management of species in human-impacted landscapes by showing which genetic metrics are best to identify immediate loss in genetic diversity and how to evaluate the effectiveness of any mitigation measures.</span></p>
Genetic and phenotypic data of sexual cross
<p>Genotypic and phenotypic data of offspring from a sexual cross of <em>Aspergillus fumigatus</em> strains AfIR974 and AfIR964. Genotypic data was obtained from Illumina whole genome sequencing off all progeny, and then filtered as described in Auxier et al. 2023. Phenotypic data consists of growth on different nitrogen sources (nitrate (NO3), nitrite (NO2), and hypoxanthine (hx)), which can be used to map the cnx and nia mutations introduced into the two parents using UV mutagenesis. Growth of each offspring was also assessed on 25, 50, and 100 µg/mL of acriflavine as binary states of either growth or no growth.</p> <p>Additionally, the data from Stapley et al. 2017, https://doi.org/10.1098/rstb.2016.0455, is presented in a more useful csv format, which was used in the publication of Auxier et al. 2023.</p> <p>Finally, data from Fernandes et al. 2018, https://doi.org/10.1073/pnas.171307811, was subsetted to include relevant species for comparison in Auxier et al. 2023.</p> <p><span>https://doi.org/10.1073/pnas.171307811</span></p>
Cross-species analysis of genetic architecture and polygenic risk scores for non-contact ACL rupture in dogs and humans
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Data from: Assessing completeness of cancer family history in EHRs using genetically-defined kinships: a cross-sectional study
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Cross-species transcriptomics uncovers genes underlying genetic accommodation of developmental plasticity in spadefoot toads
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Crossing design shapes patterns of genetic variation in synthetic recombinant populations of Saccharomyces cerevisiae
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Immediate and long-term genetic consequences of linear transport infrastructure: Can fauna crossing mitigate its cost?
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Data from: Cross ocean-basin population genetic dynamics in a pelagic top predator of high conservation concern, the oceanic whitetip shark, Carcharhinus longimanus
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Data from Koch et al. 2020 Evolution: Genetic variance in fitness and its cross-sex covariance predict adaptation during experimental evolution.
<p><strong>Abstract</strong></p> <p>In presence of rapid environmental changes, it is of particular importance to assess the adaptive potential of populations, which is mostly determined by the additive genetic variation (V<sub>A</sub>) in fitness. In this study we used <em>Tribolium castaneum </em>(red flour beetles) to investigate its adaptive potential in three new environmental conditions (Dry, Hot, Hot-Dry). We tested for potential constraints that might limit adaptation, including negative genetic covariance between female and male fitness. Based on V<sub>A</sub> estimates for fitness, we expected the highest relative fitness increase in the most stressful condition Hot-Dry and similar increases in single stress conditions Dry and Hot. High adaptive potential in females in Hot was reduced by a negative covariance with male fitness. We tested adaptation to the three conditions after 20 generations of experimental evolution and found that observed adaptation mainly matched our predictions. Given that body size is commonly used as a proxy for fitness, we also tested how this trait and its genetic variance (including non-additive genetic variance) were impacted by environmental stress. In both traits, variances were sex and condition dependent, but they differed in their variance composition, cross-sex and cross-environment genetic covariances, as well as in the environmental impact on V<sub>A</sub>.</p> <p> </p> <p><strong>Method</strong></p> <p><em>Strain and environmental conditions</em></p> <p>We used the <em>Tribolium castaneum</em> Cro1 strain collected from a wild population in 2010 and adapted to lab standard conditions (33°C, 70% relative humidity) for more than 20 generations. Beetles were kept in 24h darkness on organic wheat flour mixed with 10% organic baker's yeast. We sterilized flour and yeast by heating them for 12h at 80°C before use. To test for adaptation to new environmental conditions we used replicate lines and exposed them to three treatments and Control conditions. The conditions in the treatments were: Dry: 33°C and 30% relative humidity; Hot: 37°C and 70% r. h.; Hot-Dry: 37°C and 30% r. h.</p> <p> </p> <p><em>Crossing, fitness assay and measurement of body size</em></p> <p>In order to be able to estimate genetic variances, we applied a split-brood paternal half-sib breeding design. We produced 147 half-sib families by mating virgin males to three virgin females. Half- as well as full-sib families were split across all conditions. Male and female offspring (four females and two males per full-sib family and condition) were separated at the pupal stage and transferred to 10 mL tubes with 1 g of medium and remained there until they were used for the fitness assay eight weeks later.</p> <p>To estimate fitness, we mated each virgin male with two unrelated virgin females from the same condition in 15mL tube with 1g medium. The male was removed after 24h and females transferred into two separate tubes. Females were removed from the tubes after one week of egg laying, and 9g medium was added to provide food for the developing offspring. After five weeks the number of adult offspring was counted. While we conducted the matings for the fitness assay, we followed a specific crossing design and always crossed two pairs of full-sib families. Individuals resulting from these crosses (the F2 genaration) were double first cousins.</p> <p>Body size was measured in the F2, i.e. in the offspring of beetles that were used for the fitness assay. To estimate body size, we used the centroid size of the abdominal segment IV as proxy for total size since it can be measured more accurately than dry weight in very small insects and shows a high correlation with body mass.</p> <p> </p> <p><em>Experimental evolution</em></p> <p>We used ten replicate lines per condition originating from the same ancestral population (Cro1) and let them adapt for 20 generations. Each new generation was set up by randomly selecting 120 pupae and placing them into a new vial with 70g medium. One selection line in Dry became extinct. Adult beetles of generation 20 from all selection lines were transferred to control conditions, in which they stayed for one week to mate and lay eggs. After removal of the adults, we waited until their offspring had reached the pupal stage and separated males and females. These individuals (generation 21) developed completely in control conditions. When they had reached the adult stage, each virgin male was mated with a virgin female of the same selection line and their offspring was transferred to all four conditions in the egg stage, resulting in full-sib families split across all conditions . As soon as these offspring (generation 22) had reached the pupal stage, males and females were separated. To compare fitness of different selection lines and test for adaptation, a virgin male and a virgin female of the same selection line in the same condition, but from different families were mated and the number of adult offspring produced within four days of mating and egg laying was used as a fitness estimate.</p> <p> </p> <p> </p> <p><strong>Files</strong></p> <p>This data publication contains the following files:</p> <p>Fitness_data.txt: Fitness data (offspring number) of the first generation in new environmental conditions.</p> <p>ANIMAL: ID of female individuals; male: ID of mating partner, father of the offspring; fitness: number of adult offspring per female produced within one week of egg laying; condition: condition under which the individuals grew up, mated, reproduced (CTL: control, D: dry, H: hot, HD: hot-dry, see Methods for details); MOTHER: mother of the female; Batch: samples where fitness assay was started on the same day</p> <p> </p> <p>Size_data.txt: Centroid size of abdominal segment IV.</p> <p>ANIMAL: ID of measured individuals; condition: condition under which the individuals grew up (CT: control, D: dry, H: hot, HD: hot-dry, see Methods for details); sex: sex of individuals (f: female, m: male); AS: centroid size [Pixel] of abdominal segment IV; Mother: ID of their mother; batch: Batches represent individuals that grew up at the same time, and thus accounts for variations in the medium or lab temperature</p> <p> </p> <p>Pedigree.txt: Pedigree of measured individuals.</p> <p>ANIMAL: individual ID ; MOTHER: mother ID; FATHER: father ID</p> <p> </p> <p>Transplant_data.txt: Offspring number of different selection lines under different conditions after 20 generations of experimental evolution.</p> <p>Line: Selection-line ID; Selection: condition, in which the selection-line spent 20 generations (CT: Control; D: Dry; Hot; HD: Hot-Dry); Fam: each selection line consisted of several families; Treatment: condition, in which offspring number was measured; offspring: number of adult offspring that a female produced within four days of egg-laying</p>
Strong and weak cross-sex correlations govern the quantitative-genetic architecture of social group choice in Drosophila melanogaster
<p><span><span><span><span><span><span><span><span><span><span><span>When genotypes differ in niche-constructing traits, genotypes are expected to differ in which environments they experience, providing a novel causal relationship between genotypes, environments, and behavior. Such genetic variation in niche construction (or, more precisely, environment construction) is predicted to be especially important for social environments, yet the quantitative-genetic parameters governing such variation is still poorly understood. Here, we examine genetic variation and cross-sex genetic correlations for social environment-constructing behaviors. We focus on whether genetic variation in patch use—the tendency to spend time near food patches where conspecifics may be present—and group-size preference—the specific group size chosen when individuals are affiliating—is correlated or decoupled across sexes in the fruit fly, <i>Drosophila melanogaster</i>. Across three choice treatments, we find genotype and sex differences in how much time individuals spend near patches, and which group sizes they prefer. We find that the genetic basis of patch use is strongly coupled across sexes, whereas the genetic basis of group-size preference is completely <i>de</i>coupled across sexes. We discuss how these findings augment and complicate our understanding of the evolutionary genetics of social behaviors.</span></span></span></span></span></span></span></span></span></span></span></p>
Data from: Effects of arbuscular mycorrhizal fungi on aboveground tri-trophic interactions are contingent upon plant genetic effects of cross type in the perennial herb Ruellia nudiflora
1.- Recent work has improved our understanding of the linkages between above- and below-ground interactions mediated by plants. However, relatively few of the studies conducted thus far have focused on muli-trophic interactions (i.e. beyond two trophic levels) and the influence of plant genetic intra-specific variation on these dynamics has rarely been addressed. 2.- We tested the effect of arbuscular mycorrhizal fungi (AMF) on above-ground tri-trophic interactions associated with the canopy of the perennial herb <i></i>Ruellia nudiflora<i></i>, and further determined whether genetic effects due to cross type (i.e. wether a plant originated from from self- or cross-pollination) influenced these interactions. 3.- We propagated plants originating from self- or cross-pollination, and within each category inoculated half of the plants with AMF. We subsequently established a common garden where plants were exposed to naturally occurring seed-eating caterpillars and their parasitoids. We measured plant growth, fruit output, calculated the proportion of attacked fruits by the caterpillar and the proportion of parasitized caterpillars, and also estimated the proportion of "rescued" seeds by parasitoids representing an indirect positive effect of the third trophic level on the plant by reducing caterpillar consumption. 4.- AMF drove 18% and 15% increases in plant growth and fruit output respectively, and drove a 25% reduction in caterpillar fruit attack, but did not influence parasitism or parasitoid seed "rescue". In contrast, cross type did not influence growth, fruit number, herbivore attack, parasitism, or seed rescue. More importantly, however, we found a significant AMF by cross type interaction on caterpillar attack where AMF significantly reduced fruit attack (by 30%) in progeny from cross-pollination but did not influence herbivory in progeny from self-pollination. 5.- Synthesis. Results indicate that AMF effects on above-ground interactions are contingent upon plant intra-specific variation originating from cross type, which is likely a common source of variation in associated interactions for plants with mixed mating systems. Further studies examining plant-mediated below- and aboveground interactions should consider the influence of specific sources of plant genetic variation, as well as address the consequences of such dynamics for interactions beyond two trophic levels.
Data from: Crossing the uncrossable: novel trans-valley biogeographic patterns revealed in the genetic history of low dispersal mygalomorph spiders (Antrodiaetidae, Antrodiaetus) from California
Antrodiaetus riversi is a dispersal-limited, habitat specialized mygalomorph spider species endemic to mesic woodlands of northern and central California. Here we build upon prior phylogeographic research using a much larger geographic sample and include additional nuclear genes, providing more detailed biogeographic insights throughout the range of this complex. Of particular interest is the uncovering of unexpected and replicated trans-valley biogeographic patterns, where in two separate genetic clades western haplotypes in the California south Coast Ranges are phylogenetically closely-related to eastern haplotypes from central and northern Sierran foothills. In both instances, these trans-valley phylogenetic patterns are strongly supported by multiple genes. These western and eastern populations are currently separated by the Central Valley, a well-recognized modern-day and historical biogeographic barrier in California. For one clade the directionality is clearly northeast to southwest, and all available evidence is consistent with a jump dispersal event estimated at 1.2-1.3 million years ago. During this time period, paleogeographic data indicate that northern Sierran rivers emptied to the ocean in the south Coast Ranges, rather than at the San Francisco Bay. For the other trans-valley clade genetic evidence is less conclusive regarding the mechanism and directionality of biogeographic exchange, although the estimated timeframe is similar (approximately 1.8 Ma). Despite the large number of biogeographic studies previously conducted in central California, to the best of our knowledge no prior studies have discussed or revealed a northern Sierran to south Coast Range biogeographic connection. This uniqueness may reflect the low-dispersal biology of mygalomorph spiders, where "post-event" gene exchange rarely erases historical biogeographic signal.
Data from: Effect of microsatellite selection on individual and population genetic inferences: an empirical study using cross-specific and species-specific amplifications
Although whole-genome sequencing is becoming more accessible and feasible for nonmodel organisms, microsatellites have remained the markers of choice for various population and conservation genetic studies. However, the criteria for choosing microsatellites are still controversial due to ascertainment bias that may be introduced into the genetic inference. An empirical study of red deer (Cervus elaphus) populations, in which cross-specific and species-specific microsatellites developed through pyrosequencing of enriched libraries, was performed for this study. Two different strategies were used to select the species-specific panels: randomly vs. highly polymorphic markers. The results suggest that reliable and accurate estimations of genetic diversity can be obtained using random microsatellites distributed throughout the genome. In addition, the results reinforce previous evidence that selecting the most polymorphic markers leads to an ascertainment bias in estimates of genetic diversity, when compared with randomly selected microsatellites. Analyses of population differentiation and clustering seem less influenced by the approach of microsatellite selection, whereas assigning individuals to populations might be affected by a random selection of a small number of microsatellites. Individual multilocus heterozygosity measures produced various discordant results, which in turn had impacts on the heterozygosity-fitness correlation test. Finally, we argue that picking the appropriate microsatellite set should primarily take into account the ecological and evolutionary questions studied. Selecting the most polymorphic markers will generally overestimate genetic diversity parameters, leading to misinterpretations of the real genetic diversity, which is particularly important in managed and threatened populations.
Insights into the genetic architecture of sexual dimorphism from an interspecific cross between two diverging Silene (Caryophyllaceae) species
<p class="Cuerpo">The evolution of sexual dimorphism in species with separate sexes is influenced by the resolution of sexual conflicts creating sex differences through genetic linkage or sex-biased expression. Plants with different degrees of sexual dimorphism are thus ideal to study the genetic basis of sexual dimorphism. In this study we explore the genetic architecture of sexual dimorphism between <i>Silene latifolia </i>and<i> S. dioica. </i>These species have chromosomal sex determination and differ in the extent of sexual dimorphism. To test whether QTL for sexually dimorphic traits have accumulated on the sex chromosomes and to quantify their contribution to species differences, we create a linkage map and performed QTL analysis of life history, flower and vegetative traits using an unidirectional interspecific F2 hybrid cross. We found support for an accumulation of QTL on the sex chromosomes and that sex differences explained a large proportion of the variance between species, suggesting that both natural and sexual selection contributed to species divergence. Sexually dimorphic traits that also differed between species displayed transgressive segregation. We observed a reversal in sexual dimorphism in the F2 population, where males tended to be larger than females, indicating that sexual dimorphism is constrained within populations but not in recombinant hybrids. This study contributes to the understanding of the genetic basis of sexual dimorphism and its evolution in <i>Silene.</i></p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
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