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21
datasets available to search
ShareScore release 0.9.0
Dataset results
21 results for “genetic technology”
Supplementary dataset to publication: Oxford nanopore technologies - a valuable tool to generate whole-genome sequencing data for in silico serotyping and the detection of genetic markers in Salmonella, Thomas et al 2023
<p>Bacteria of the genus <em>Salmonella</em> pose a major risk to livestock, the food economy, and public health. <em>Salmonella</em> infections are one of the leading causes of food poisoning. The identification of serovars of <em>Salmonella</em> achieved by their diverse surface antigens is essential to gain information on their epidemiological context. Traditionally, slide agglutination has been used for serotyping. In recent years, whole-genome sequencing (WGS) followed by <em>in silico</em> serotyping has been established as an alternative method for serotyping and the detection of genetic markers for <em>Salmonella</em>. Until now, WGS data generated with Illumina sequencing are used to validate <em>in silico</em> serotyping methods. Oxford Nanopore Technologies (ONT) opens the possibility to sequence ultra-long reads and has frequently been used for bacterial sequencing. In this study, ONT sequencing data of 28 <em>Salmonella</em> strains of different serovars with epidemiological relevance in humans, food, and animals were taken to investigate the performance of the <em>in silico</em> serotyping tools SISTR and SeqSero2 compared to traditional slide agglutination tests. Moreover, the detection of genetic markers for resistance against antimicrobial agents, virulence, and plasmids was studied by comparing WGS data based on ONT with WGS data based on Illumina. Based on the ONT data from flow cell version R9.4.1, <em>in silico</em> serotyping achieved an accuracy of 96.4 and 92% for the tools SISTR and SeqSero2, respectively. Highly similar sets of genetic markers comparing both sequencing technologies were identified. Taking the ongoing improvement of basecalling and flow cells into account, ONT data can be used for <em>Salmonella in silico</em> serotyping and genetic marker detection.</p>
Dataset: Genetic Technologies Limited (GENE) Stock Performance
This dataset provides historical stock market performance data for specific companies. It enables users to analyze and understand the past trends and fluctuations in stock prices over time. This information can be utilized for various purposes such as investment analysis, financial research, and market trend forecasting.
Waste not, want not: microsatellites remain an economical and informative technology for conservation genetics
<p>Comparisons of microsatellite and single-nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, calling into question the continued utility of microsatellites in population and landscape genetics. Yet highly polymorphic markers may be of value in species that have reduced genetic variation. This study repeated analyses previously done using microsatellites with SNPs developed from ddRAD sequencing in the black-capped vireo source-sink system. SNPs provided greater resolution of genetic diversity, population differentiation, and migrant detection but could not reconstruct parentage relationships due to insufficient heterozygosities. The biological inferences made by both sets of markers were similar: asymmetrical gene flow from source sites to the remaining sink sites. With the landscape genetic analyses, we found different results between the two molecular markers, but associations of the top environmental features (riparian, open habitat, agriculture, and human development) with dispersal estimates were shared between marker types. Despite the higher precision of SNPs, we find that microsatellites effectively uncover population processes and patterns and are superior for parentage analyses in this species with reduced genetic diversity. This study illustrates the continued applicability and relevance of microsatellites in population genetic research.</p>
Waste not, want not: microsatellites remain an economical and informative technology for conservation genetics
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Dataset: T-DNA characterization of genetically modified 3-R-gene late blight resistant potato events with a novel procedure utilizing the Samplix Xdrop® Enrichment Technology
<p>Before commercialization of genetically modified crops, the events carrying the novel DNA must be thoroughly evaluated for agronomic, nutritional, and molecular characteristics. Over the years, Polymerase Chain Reaction-based methods, Southern blot, and short-read sequencing techniques have been utilized for collecting molecular characterization data. Multiple genomic applications are necessary to determine the insert location, flanking sequence analysis, characterization of the inserted DNA, and determination of any interruption of native genes. These techniques are time-consuming and labor-intensive, making it difficult to characterize multiple events. Current advances in sequencing technologies are enabling whole genomic sequencing of modified crops to obtain full molecular characterization. However, in polyploids, such as the tetraploid potato, it is a challenge to obtain whole genomic sequencing coverage that meets regulatory approval of the genetic modification. Here we describe an alternative to labor-intensive applications with a novel procedure using Samplix Xdrop® enrichment technology and next-generation Nanopore sequencing technology to more efficiently characterize the T-DNA insertions of four genetically modified potato events developed by the Feed the Future Global Biotech Potato Partnership: DIA_MSU_UB015, DIA_MSU_UB255, GRA_MSU_UG234 and GRA_MSU_UG265 (derived from regionally important varieties Diamant and Granola). Using the Xdrop® /Nanopore technique, we obtained a very high sequence read coverage within the T-DNA and junction regions. In three of the four events, we were able to use the data to confirm single T-DNA insertions, identify insert locations, identify flanking sequences, and characterize the inserted T-DNA. We further used the characterization data to identify native gene interruption and confirm the stability of the T-DNA across clonal cycles. These results demonstrate the functionality of using the Xdrop® /Nanopore technique for T-DNA characterization. This research will contribute to meeting regulatory safety and regulatory approval requirements for commercialization with small shareholder farmers in target countries within our partnership.</p>
Dataset: T-DNA characterization of genetically modified 3-R-gene late blight resistant potato events with a novel procedure utilizing the Samplix Xdrop® Enrichment Technology
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Highly Efficient Agrobacterium rhizogenes-mediated Genetic Transformation Technology Bypassing Tissue Culture in Citrus
<p>Highly efficient genetic transformation technology is of great significance for the gene function analysis and precision breeding of crops. However, the most commonly used genetic transformation technology mediated by <em>Agrobacterium tumefaciens</em> in plants especially xylophyta is time-consuming and inefficient, which seriously hinders the progress of gene function analysis. In this study, a simple and highly efficient genetic transformation technology mediated by <em>Agrobacterium rhizogenes</em> bypassing tissue culture is described. Only 2~8 weeks were required for the whole workflow, with an average successful transformation ratio of 57% in shoots from adult plants. By using this technology, we successfully transferred plasmids containing gRNA, Cas9, and other exogenous genes into citrus, and achieved genome editing on target loci and overexpressed multiple foreign genes at the same time. In this method, a student could simultaneously conduct genome editing experiment using 10 plasmids targeting different genome positions and finally obtained all the corresponding transformants with target DNA knocked out, indicating that <em>A. rhizogenes</em>-mediated genome editing technology is highly efficient. In addition, <em>A. rhizogenes</em> can be used for direct viral vector inoculation on citrus bypassing the step of viron enrichment in tobacco, which facilitates the experiment operation of virus-induced gene silence (VIGS) and foreign gene expression. In summary, we established a highly efficient genetic transformation technology bypassing tissue culture in citrus, which can be used for genome editing, gene overexpression, and virus-mediated gene function analysis. We anticipate that by removing high cost, heavy workload, long experiment period and other technical obstacles, this genetic transformation technology will be a valuable tool for routine investigation of endogenous and exogenous genes in citrus.</p>
Choices About Genetic Testing And Learning Your Risk With Smart Technology
ClinicalTrials.gov study NCT06184867. IPD Sharing: NO. Countries: 1. Publications: 0.
Refining Information Technology Support for Genetics in Medicine
ClinicalTrials.gov study NCT01225978. IPD Sharing: Not stated. Countries: 2. Publications: 1.
Mobile Phone Technology to Increase Genetic Counseling
ClinicalTrials.gov study NCT02877862. IPD Sharing: NO. Countries: 1. Publications: 1.
Research on key generic technology prediction based on graph neural networks under the perspective of patent citation - An example from the field of genetic engineering
<p>In this research, we adopted graph neural network models for key generic prediction based on cited patent data. Through the construction of the patent citation network and the design of a key generic evaluation system, 20879 relevant patents and 51,610 irrelevant patents were screened out. Further, we utilized the LDA topic model to interpret technical topics at a finer granularity. Finally, to test the effectiveness of this method, we took the field of genetic engineering as an example for key generic technology prediction, with an accuracy rate of 95%.</p>
Technology Development for Noninvasive Prenatal Genetic Diagnosis Using Whole Fetal Cells From Maternal Peripheral Blood
ClinicalTrials.gov study NCT04285814. IPD Sharing: NO. Countries: 1. Publications: 0.
Access to Genetic Information Leveraging Innovative Technology (AGILITY)
ClinicalTrials.gov study NCT05159011. IPD Sharing: Not stated. Countries: 0. Publications: 7.
Comparative evaluation of genotyping technologies for investigative genetic genealogy in sexual assault casework
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Utilizing Non-Invasive Fibroscan® Technology to Identify Genetic Markers for Fatty Liver Progression
ClinicalTrials.gov study NCT02267148. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Enhanced Genetic Awareness and Genetic Evaluation for Men Through Technology, ENGAGEMENT Study
ClinicalTrials.gov study NCT04789018. IPD Sharing: NO. Countries: 1. Publications: 0.
Face Anthropometric Pattern Recognition Technology for Computer Aided Diagnosis of Human Genetic Disorders.
ClinicalTrials.gov study NCT00705055. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Patient Forward Access to Clinical and Technological Research: Genetic Influences on Cancer and Atopic Dermatitis
ClinicalTrials.gov study NCT04362852. IPD Sharing: Not stated. Countries: 1. Publications: 0.
A Study of Preimplantation Genetic Screening With Next Generation Sequencing Technology on Advanced Age Women
ClinicalTrials.gov study NCT02868528. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Phenotypic and Genetic Properties in Males at Risk for X-linked Hypohidrotic Ectodermal Dysplasia: Evaluation of an Early Diagnosis Technology and Tests to Assess Nutritional Status
ClinicalTrials.gov study NCT01629940. IPD Sharing: Not stated. Countries: 1. Publications: 0.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.