Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
98
datasets available to search
ShareScore release 0.9.0
Dataset results
98 results for “heat shock response”
Induction of Sis1 promotes fitness but not feedback in the heat shock response
<div class="page"> <div class="layoutArea"> <div class="column"> <p>The heat shock response (HSR) controls expression of molecular chaperones to maintain protein homeostasis. Previously, we proposed a feedback loop model of the HSR in which heat-denatured proteins sequester the chaperone Hsp70 to activate the HSR, and subsequent induction of Hsp70 deactivates the HSR. However, recent work has implicated newly synthesized proteins (NSPs) – rather than unfolded mature proteins – and the Hsp70 co-chaperone Sis1 in HSR regulation, yet their contributions to HSR dynamics have not been determined. Here we generate a new mathematical model that incorporates NSPs and Sis1 into the HSR activation mechanism, and we perform genetic decoupling and pulse-labeling experiments to demonstrate that Sis1 induction is dispensable for HSR deactivation. Rather than providing negative feedback to the HSR, transcriptional regulation of Sis1 by Hsf1 promotes fitness by coordinating stress granules and carbon metabolism. These results support an overall model in which NSPs signal the HSR by sequestering Sis1 and Hsp70, while induction of Hsp70 – but not Sis1 – attenuates the response.</p> </div> </div> </div>
Induction of Sis1 promotes fitness but not feedback in the heat shock response
Open the record for dataset details and reuse information.
Transient intracellular acidification regulates the core transcriptional heat shock response
<p>Heat shock induces a conserved transcriptional program regulated by heat shock factor 1 (Hsf1) in eukaryotic cells. Activation of this heat-shock response is triggered by heat-induced misfolding of newly synthesized polypeptides, and so has been thought to depend on ongoing protein synthesis. Here, using the budding yeast <em>Saccharomyces cerevisiae</em>, we report the discovery that Hsf1 can be robustly activated when protein synthesis is inhibited, so long as cells undergo cytosolic acidification. Heat shock has long been known to cause transient intracellular acidification which, for reasons which have remained unclear, is associated with increased stress resistance in eukaryotes. We demonstrate that acidification is required for heat shock response induction in translationally inhibited cells, and specifically affects Hsf1 activation. Physiological heat-triggered acidification also increases population fitness and promotes cell cycle reentry following heat shock. Our results uncover a previously unknown adaptive dimension of the well-studied eukaryotic heat shock response. </p>
Transient intracellular acidification regulates the core transcriptional heat shock response
Open the record for dataset details and reuse information.
Membrane lipid metabolism, heat shock response, and energy costs mediate the interaction between acclimatization and heat hardening response
<p>Thermal plasticity on different timescales, including acclimation/acclimatization and heat hardening response – a rapid adjustment for thermal tolerance after a nonlethal thermal stress, can interact on organisms to improve the resilience to thermal stress. However, little is known about the physiological mechanisms mediating this interaction. To investigate underpinnings of heat hardening responses after acclimatization in warm season, we measured thermal tolerance plasticity, compared transcriptomic and metabolomic changes after heat hardening at 33 or 37<sup>o</sup>C followed by recovery of 3 h or 24 h in an intertidal bivalve <i>Sinonovacula constricta</i>. The clams showed explicit heat hardening responses after acclimatization in warm season. The higher inducing temperature (37<sup>o</sup>C) caused a less effective heat hardening effect than the inducing temperature that was closer to seasonal maximum temperature (33<sup>o</sup>C). Metabolomic analysis highlighted the elevated contents of membrane glyceropholipids in all heat hardened clams, which may help to maintain structure and function of membrane. Heat shock proteins (HSPs) tended to be up-regulated after heat hardening at 37<sup>o</sup>C but not at 33<sup>o</sup>C, indicating that there was no complete dependency of heat hardening effects on up-regulated HSPs. Enhanced energy metabolism and decreased energy reserves were observed after heat hardening at 37<sup>o</sup>C, suggesting more energy costs during exposure to higher inducing temperature which may restrict heat hardening effects. These results highlighted the mediating role of membrane lipid metabolism, heat shock responses and energy costs in the interaction of heat hardening response and seasonal acclimatization, and benefit the mechanistic understanding of evolutionary change and thermal plasticity during global climate change.</p>
Membrane lipid metabolism, heat shock response, and energy costs mediate the interaction between acclimatization and heat hardening response
Open the record for dataset details and reuse information.
Data from: Inbreeding interferes with the heat-shock response
Open the record for dataset details and reuse information.
A multi-omics dataset of heat-shock response in the yeast RNA transport protein Mip6
GEO Series GSE135568. Saccharomyces cerevisiae. 67 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
HSF1-dependent and -independent regulation of the mammalian in vivo heat shock response and its impairment in Huntington’s disease
GEO Series GSE95602. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide Transcription Response of Staphylococcus epidermidis to Heat Shock and Medically-Relevant Glucose Levels
GEO Series GSE261664. Staphylococcus epidermidis ATCC 12228. 28 samples. Type: Expression profiling by high throughput sequencing.
Systemic Comparison of Heat Shock Response Induced by Heat Shock and a Proteasome Inhibitor in Mouse Fibrosarcoma Cells and Their Thermotolerant Counterparts
GEO Series GSE24197. Mus musculus. 32 samples. Type: Expression profiling by array.
Transcriptome-wide changes in HeLa cell line investigated in response to Heat-shock stress
GEO Series GSE26776. Homo sapiens. 6 samples. Type: Expression profiling by array.
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response [time course]
GEO Series GSE299855. Saccharomyces cerevisiae. 35 samples. Type: Other.
Interplay between RNA interference and heat shock response systems in Drosophila melanogaster
GEO Series GSE76844. Drosophila melanogaster. 24 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Measuring the dependence of the yeast heat shock response on intracellular pH during stress
GEO Series GSE143292. Saccharomyces cerevisiae. 8 samples. Type: Expression profiling by high throughput sequencing.
A microsporidia possesses robust transcriptional response to heat shock impacting diverse cellular processes despite lack of HSF
GEO Series GSE128364. Apis mellifera; Vairimorpha ceranae. 6 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profiling (RNA-seq) in wild-type and bdrs triple mutant Arabidopsis seedlings in response to light or to a heat shock
GEO Series GSE112442. Arabidopsis thaliana. 30 samples. Type: Expression profiling by high throughput sequencing.
Measuring the dependence of the yeast heat shock response on intracellular pH and translation during stress
GEO Series GSE152916. Saccharomyces cerevisiae. 22 samples. Type: Expression profiling by high throughput sequencing.
Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [Ribo-Seq]
GEO Series GSE265859. Arabidopsis thaliana. 4 samples. Type: Other.
Specific phosphorylation of histone demethylase KDM3A determines target gene expression in response to heat shock
GEO Series GSE62309. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.