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2 results for “hsp65”
The hsp65 metabarcoding DNA sequence database for taxonomic allocations using the Mothur (Version 1.0.0)
<ul> <li>The <em>hsp65</em> gene codes for an Heat Shock Protein (Telenti et al., 1993) and is widespread in the Actinobacteria phylum. It is well suited for the species allocation of the Nocardia genus (Rodriguez-Nava et al., 2006).</li> <li>The <em>hsp65</em> database, named ACTIhsp65, was designed to apply the <em>hsp65</em>-metabarcoding analytical scheme published in Vautrin et al. (2021). It includes the full <em>hsp65</em> identifiers, GenBank accession numbers, complete taxonomic records (domain down to strain code) of about 401 nucleotide-long <em>hsp65</em> sequences of 1066 unique taxa belonging to 198 genera.</li> <li>Nucleotide sequences of <em>hsp65</em> (range: 165-565 nucleotides) were either retrieved from public repositories (GenBank) or made available by Veronica Rodriguez-Nava.Vautrin et al. (2021) described the PCR and high throughput Illumina Miseq DNA sequencing procedures used to produce <em>hsp65</em> sequences.</li> <li>ACTIhsp65 V1.0.0 (June 2018 release) is made available under the Creative Commons Attribution 4.0 International Licence. It can be used for the taxonomic allocations of <em>hsp65 </em>sequences down to the species.</li> </ul>
Table of hsp65 OTUs (cutoff 99%), their inferred taxonomic allocations according to the hsp65 database and, for selected OTUs, closest species obtained from GenBank (BLAST) with percent identity.
<p>This table is part of the paper intitled "Comparison of Actinobacteria communities from human-impacted and pristine karst caves"</p>
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