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Dataset results
271 results for “immunoprecipitation”
IgLON5 immunoprecipitation files of binding partners in rat granullar cells of the cerebellum
<p class="MsoNormal"><span>Background: Anti-IgLON5 disease is a rare neurological disorder characterized by autoantibodies against IgLON5, and pathological evidence of neurodegeneration. IgLON5 is a cell adhesion molecule but its physiological function is unknown. Our aim was to investigate the IgLON5 interactome and to determine if IgLON5 antibodies (IgLON5-abs) affect these protein interactions.</span></p> <p class="MsoNormal"><span>Methods: IgLON5 interactome was investigated by mass spectrometry sequencing of proteins immunoprecipitated by IgLON5 autoantibodies using cultures of rat cerebellar granular neurons (CGNs). Shedding of IgLON5 was explored using HEK cells transfected with human IgLON5 plasmid and in CGNs. Interactions of IgLON5 with identified binding partners and IgLON5-abs effects were confirmed by immunofluorescence in transfected HEK cells and rat hippocampal neurons.</span></p> <p class="MsoNormal"><span>Results: Patients' IgLON5 antibodies co-precipitated all members of the IgLON family and three 3 additional surface proteins. IgLON5 predominantly establishes homomeric and heteromeric <em>cis</em> (within the cell) and <em>trans </em>(between cells)-interactions with other IgLON family members and undergoes spontaneous ectodomain shedding. Antibodies from patients with anti-IgLON5 disease prevent trans-interactions in hippocampal neurons independently of the IgLON5 IgG subclass distribution. </span></p> <p class="MsoNormal"><span>Conclusions: We show a potentially novel pathogenic mechanism of IgLON5-abs that consists in blocking IgLON5 interactions with its binding partners. These findings extend our knowledge about the physiological role of IgLON5 and pave the way to future understanding of the pathological mechanisms of anti-IgLON5 disease.</span></p>
Quantitative Multiplex Immunoprecipitation (QMI) analysis of protein interaction network rearrangements during homeostatic plasticity
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IgLON5 immunoprecipitation files of binding partners in rat granullar cells of the cerebellum
Open the record for dataset details and reuse information.
RNA immunoprecipitation and sequencing of aminoacyl-tRNA synthetase-specific genes
GEO Series GSE120182. Homo sapiens. 14 samples. Type: Expression profiling by high throughput sequencing.
Next Generation Sequencing of CELF6-HA/YFP Crosslinking Immunoprecipitation Samples and Controls
GEO Series GSE118623. Mus musculus. 14 samples. Type: Expression profiling by high throughput sequencing.
Chromatin-immunoprecipitation followed by sequencing (ChIPseq) of SOX17 and FOXA2 in yolk-sac tumor cell lines
GEO Series GSE276475. Homo sapiens. 16 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Enhanced crosslinking and immunoprecipitation of SERBP1 in Hela cells
GEO Series GSE156015. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Chromatin Immunoprecipitation of E2A-PBX1 in the cell line 697
GEO Series GSE52653. Homo sapiens. 3 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Genome-wide chromatin-immunoprecipitation studies of sympathetic neurons reveals critical direct targets of ISL1 and suggests potential co-factor families for ISL1 action in sympathetic neurons
GEO Series GSE93304. Mus musculus. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Photoactivatable-Ribonucleotide-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) of RNA-binding proteins (RBPs), IGF2BP1 and IGF2BP3 in the K562 CML-derived cell line
GEO Series GSE138063. Homo sapiens. 4 samples. Type: Other.
Effect of SAT1 depletion on H3K27ac alterations in the ovarian cancer cell line SKOV3 in detached conditions by chromatin immunoprecipitation and sequencing (ChIP-seq) analysis
GEO Series GSE267703. Homo sapiens. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase (BrdU)
GEO Series GSE71050. Saccharomyces cerevisiae. 64 samples. Type: Expression profiling by high throughput sequencing; Other.
Chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq) detection of histone H3 lysine 14 acetylation (H3K14ac) and KAT7 (HBO1) in E14.5 mouse neural stem and progenitor cells (NSPCs)
GEO Series GSE216459. Mus musculus. 13 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
A Single-copy Knock In Translating Ribosome ImmunoPrecipitation (SKI TRIP) tool kit for tissue specific profiling of actively translated mRNAs in C. elegans.
GEO Series GSE195601. Caenorhabditis elegans. 54 samples. Type: Expression profiling by high throughput sequencing.
Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [miRNA-seq]
GEO Series GSE184534. Homo sapiens. 5 samples. Type: Non-coding RNA profiling by high throughput sequencing.
mRNA expression profiling after Ago2-immunoprecipitation (IP) in unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts of two control subjects
GEO Series GSE86183. Homo sapiens. 16 samples. Type: Other.
Chromatin immunoprecipitation and expression analysis in WT and hrr25 mutant cells
GEO Series GSE107166. Saccharomyces cerevisiae. 30 samples. Type: Genome binding/occupancy profiling by genome tiling array; Expression profiling by high throughput sequencing.
RNA immunoprecipitation (RIP) of IGF2BP3 from pancreatic cancer cell extracts cultured on fibronectin
GEO Series GSE47597. Homo sapiens. 2 samples. Type: Other.
Chromatin immunoprecipitation assay of EVI1 in MCF7 and HCC1937 breast cancer cells untreated and everolimus-adapted
GEO Series GSE50905. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Kinesin-associated RNA: RNA co-immunoprecipitated with Kinesin IP vs RNA from Aplysia CNS
GEO Series GSE30386. Aplysia californica. 8 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.