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23 results for “iron uptake”

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zenodo52/100

PARAGON 1 - KM2112 - Iron Uptake In Situ Measurements

<p>This dataset contains measurements of in situ rates of dissolved iron (Fe) uptake collected during the PARAGON 1 expedition (KM2112) in the North Pacific Subtropical Gyre. Measurements come from daily collections of whole seawater at 150 m using trace metal clean techniques. Rates of iron uptake were measured by subsampling 250 mL aliquots of whole seawater into polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻&sup1; ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻&sup1; of Fe (Perkin Elmer). A killed blank measurement was also made by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 &micro;m polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 &micro;m filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected averages of triplicate measurements from each sample but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC.</p>

opencc-by-4.0Apr 2024View details →
zenodo52/100

PARAGON 1 - KM2112 - Iron Uptake Timecourse Incubations

<p>This dataset contains measurements of rates of dissolved iron (Fe) uptake collected during the PARAGON 1 expedition (KM2112) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Rates of iron uptake were measured at multiple time points for each experimental biological replicate by subsampling 250 mL aliquots from polycarbonate incubation bottles into new polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻&sup1; ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻&sup1; of Fe (Perkin Elmer). A killed blank measurement was made for each experimental treatment by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 &micro;m polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 &micro;m filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC. Version 2 corrects formatting errors in the timestamp.</p>

opencc-by-4.0Apr 2024View details →
zenodo52/100

PARAGON 2 - KM2209 - Iron Uptake Timecourse Incubations

<p>This dataset contains measurements of rates of dissolved iron (Fe) uptake collected during the PARAGON 2 expedition (KM2209) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Rates of iron uptake were measured at multiple time points for each experimental biological replicate by subsampling 250 mL aliquots from polycarbonate incubation bottles into new polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻&sup1; ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻&sup1; of Fe (Perkin Elmer). A killed blank measurement was made for each experimental treatment by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 &micro;m polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 &micro;m filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC.</p>

opencc-by-4.0May 2024View details →
zenodo36/100

Station ALOHA seasonal siderophore concentrations and iron uptake

<p>Seasonal total siderophore concentrations and surface (15 m) and 300 m iron uptake from Station ALOHA.</p>

opencc-by-4.0Sep 2022View details →
ClinicalTrials.gov36/100

Oral Iron Repletion Effects On Oxygen Uptake in Heart Failure

ClinicalTrials.gov study NCT02188784. IPD Sharing: YES. Countries: 1. Publications: 2.

controlledIPD-YESFeb 2026View details →
dryad32/100

Data from: Switching between apparently redundant iron-uptake mechanisms benefits bacteria in changeable environments

Open the record for dataset details and reuse information.

publicJun 2013View details →
ClinicalTrials.gov28/100

Investigation of Iron Uptake From Micronutrient Fortified Powder Versus From Tailored Control in Milk

ClinicalTrials.gov study NCT02254460. IPD Sharing: Not stated. Countries: 1. Publications: 0.

restrictedIPD-UNDECIDEDFeb 2026View details →
geo24/100

Microbiota-derived pentanoate promotes differentiation of regulatory T cells via enhancing iron uptake in the intestine.

GEO Series GSE195607. Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2022View details →
geo24/100

Microbiota-derived pentanoate promotes differentiation of regulatory T cells via enhancing iron uptake in the intestine [scRNAseq_Treg]

GEO Series GSE195604. Mus musculus. 1 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2022View details →
geo24/100

Microbiota-derived pentanoate promotes differentiation of regulatory T cells via enhancing iron uptake in the intestine [Treg_in_vivo]

GEO Series GSE195603. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2022View details →
geo24/100

MliR, a novel regulator of the MerR family involved in iron uptake

GEO Series GSE189140. Bizionia argentinensis JUB59. 6 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenAug 2022View details →
geo24/100

A defect in iron uptake enhances the susceptibility of Cryptococcus neoformans to azole antifungal drugs.

GEO Series GSE37875. Cryptococcus neoformans H99. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMay 2013View details →
geo24/100

Microbiota-derived pentanoate promotes differentiation of regulatory T cells via enhancing iron uptake in the intestine [FeSO4_treated_Treg]

GEO Series GSE195602. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2022View details →
geo24/100

Transcriptomics and Real-Time PCR analysis reveals a cross-modulatory effect between riboflavin and iron and outlines common and specific responses to riboflavin biosynthesis and uptake in Vibrio chol

GEO Series GSE107538. Vibrio cholerae. 15 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenFeb 2018View details →
geo24/100

Acidic pH Reduces Fluconazole Susceptibility in Cryptococcus neoformans by Altering Iron Uptake and Enhancing Ergosterol Biosynthesis

GEO Series GSE291861. Cryptococcus neoformans H99. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMar 2025View details →
geo24/100

Iron uptake controls the generation of Leishmania infective forms through regulation of ROS levels .

GEO Series GSE41641. Leishmania amazonensis. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenDec 2012View details →
geo20/100

Iron uptake in yeast

GEO Series GSE3496. Saccharomyces cerevisiae. 4 samples. Type: Expression profiling by array.

openGEO-OpenOct 2005View details →
geo20/100

Expression analysis of Clostridium difficile wild type and fur (ferric uptake regulator) mutant in high iron.

GEO Series GSE69218. Clostridioides difficile 630. 12 samples. Type: Expression profiling by array.

openGEO-OpenMay 2015View details →
geo20/100

NHR-14 loss of function couples intestinal iron uptake with innate immunity in C. elegans through PQM-1 signaling

GEO Series GSE89783. Caenorhabditis elegans. 6 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenOct 2019View details →
geo20/100

Mutations in LRRK2 linked to Parkinson’s disease sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia

GEO Series GSE186559. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenOct 2021View details →

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