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4 results for “kinase fusion protein”
A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew
<p>Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders cloning of the introgressed genes. Here, we report the identification of wheat powdery mildew resistance gene <em>Pm13</em> originated from from <em>Aegilops longissima</em> using a method combining physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. <em>Pm13</em> encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of <em>Pm13</em> is validated by transient and stable transgenic complementation assays. Transient over-expression analyses in <em>Nicotiana benthamiana</em> leaves and wheat protoplasts reveal that the fragment Brace-Kinase<sub>122-476</sub> of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three α-helices in the brace region close to the N-terminus of the kinase domain.</p>
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
<p>The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with novel disease-resistance genes. Here, we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene <em>Lr9</em>, which was introduced into bread wheat from the wild grass species <em>Aegilops</em> <em>umbellulata</em>. We establish that <em>Lr9</em> encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat <em>Lr9</em> introgression line and the putative <em>Ae</em>. <em>umbellulata</em> <em>Lr9</em> donor enabled us to assemble the ~28.4-Mb <em>Lr9</em> translocation and to identify the translocation breakpoint. We likewise cloned <em>Lr58</em>, which was reportedly introgressed from <em>Ae</em>. <em>triuncialis</em>, but has an identical coding sequence compared to <em>Lr9</em>. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.</p>
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
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A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew
Open the record for dataset details and reuse information.
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