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543 results for “larval development”
Asaia spp. accelerate development of the yellow fever mosquito, Aedes aegypti, via interactions with the vertically transmitted larval microbiome
<p><strong><span>Background:</span></strong><em> Aedes aegypti</em> mosquitoes are the primary vectors of yellow fever, dengue, chikungunya and Zika virus. Control programs primarily rely on insecticide application, which encounter challenges related to efficacy and resistance evolution. Alternative strategies, such as the sterile insect technique, highly depend on efficient mass-rearing of healthy insects prior to mass release. Based on effects seen in other mosquito species, we tested the hypothesis that acetic acid bacteria <span>of the </span><em>Asaia</em> <span>genus are</span> mutualist<span>s</span> for developing <em>Ae. aegypti</em> larvae. We tested for beneficial interactions across three <em>Asaia </em>species and whether <em>Asaia</em> inoculation benefited both axenic and conventionally reared larvae. To better understand the underlying mechanisms, we characterized the larval microbiome<span> </span>using culture-based methods and 16S rRNA gene amplicon sequencing.</p> <p><strong>Results:</strong><span> <span>Even</span></span> though <em>Asaia </em>bacteria were transient members of the gut community in conventionally reared insects<span>, t</span>wo <em>Asaia </em>species accelerated larval development relative to controls.<span> Despite their transient nature, </span>the two mutualist <em>Asaia</em> species had lasting impacts on the larval microbiome, mostly by altering the relative abundance of the most dominant bacteria genera <em>Klebsiella</em> and <em>Pseudomonas</em> and other minor components<span>.</span> Axenic larvae that were inoculated with <em>Asaia </em>were dominated by this group, but always exhibited slower development than conventionally reared insects.</p> <p><strong>Conclusions:</strong> These results reveal <em>Asaia</em> as a poor mutualist for <em>Ae. aegypti</em>, with its<em> </em>positive effect on the host mediated by interactions with other bacteria. A practical application of <em>Asaia </em>for improving mass-rearing efficiency results from the acceleration of development time to pupation by a day.</p>
Raw data for Development of Germline Progenitors in Larval Queen Honeybee ovaries
<p>This repository contains raw files for images relating to a publication of honeybee ovary development. That work is Cullen, Delargy and Dearden 2024, <strong><span>Development of Germline Progenitors in Larval Queen Honeybee ovaries. </span></strong><span>The data is organised in folders relate to each figure, and is in .oir format, a raw data format produced by Olympus confocal systems. This data file format is able to be read by FIJI.</span></p>
Data from: 'A large, infrequent ecosystem subsidy (cicada carcasses) and warming additively accelerate development and increase growth of larval amphibians'
These data are from an experiment designed to quantify how ecosystem subsidies and elevated temperatures affect pond food webs, focusing on the response of frogs and the mechanisms affecting their responses. The subsidy we examined was the deposition of periodical cicada carcasses into ponds, simulating a large subsidy event that happens only every 17 years. The 7-week experiment was conducted in outdoor tanks using a factorial design with four treatments: Control (no subsidy, ambient temperature); Cicadas (cicada carcasses added in one large pulse); Warming (temperature elevated about 2.6°C above ambient); and Cicadas & Warming. The data set includes two frog response variables: time to metamorphosis (days) and size at metamorphosis (g wet mass). It also includes temperature in each tank, measured at 30-minute intervals. In addition, it includes data designed to characterize resource supply to frogs, including the abundance of periphyton and phytoplankton measured as chlorophyll concentration; periphyton and phytoplankton composition at the Division level using data from a spectrofluoroprobe; and water column nutrient concentrations, including ammonium, nitrate, total nitrogen, soluble reactive phosphorus, and total phosphorus. In addition, we estimated algal gross primary production using data on dissolved oxygen measured at 30-minute intervals, and include oxygen data here. Finally, we measured the rate at which cicada carcasses and leaf litter decomposed, and dissolved organic carbon concentrations at the end of the experiment, as these data may shed light on factors affecting nutrient supply to algae as well as ecosystem respiration rates, which are used to estimate gross primary production rates. Thus, we include data on the mass of cicada carcasses and leaf litter on several dates, as well as DOC concentrations on the last day of the experiment.
Figure 8. Metapenaeus dalli Protozoea I in Larval development of the western school prawn Metapenaeus dalli Racek, 1957 (Crustacea: Decapoda: Penaeidae) reared in the laboratory
Figure 8. Metapenaeus dalli Protozoea I (a) dorsal view; (b) first antenna; (c) second antenna; (d) mandible; (e) first maxilla; (f) second maxilla; (g) first maxilliped; (h) second maxilliped; (i) third maxilliped. Scale bars: a–c, g–i = 0.1 mm; d–f = 0.05 mm.
Figure 6. Metapenaeus dalli Nauplius V in Larval development of the western school prawn Metapenaeus dalli Racek, 1957 (Crustacea: Decapoda: Penaeidae) reared in the laboratory
Figure 6. Metapenaeus dalli Nauplius V (a) ventral view; (b) first antenna; (c) second antenna; (d) mandible. Scale bar = 0.1 mm.
Figure 2. Metapenaeus dalli Nauplius I in Larval development of the western school prawn Metapenaeus dalli Racek, 1957 (Crustacea: Decapoda: Penaeidae) reared in the laboratory
Figure 2. Metapenaeus dalli Nauplius I (a) ventral view; (b) first antenna; (c) second antenna; (d) mandible. Endopod (End.) and exopod (Ex.) denoted on second antennae and mandible. Scale bar = 0.1 mm.
Figure 14. Metapenaeus dalli Post larvae I in Larval development of the western school prawn Metapenaeus dalli Racek, 1957 (Crustacea: Decapoda: Penaeidae) reared in the laboratory
Figure 14. Metapenaeus dalli Post larvae I (a) lateral view; (b) first antenna; (c) second antenna; (d) mandible; (e) first maxilla; (f) second maxilla; (g) first maxilliped; (h) second maxilliped; (i) third maxilliped; (j) third pereiopod; (k) fifth pereiopod; (l) pleopods; (m) telson and uropods. Scale bars: a–c, g–m = 0.1 mm; d–f = 0.05 mm.
Figure 7. Metapenaeus dalli Nauplius VI in Larval development of the western school prawn Metapenaeus dalli Racek, 1957 (Crustacea: Decapoda: Penaeidae) reared in the laboratory
Figure 7. Metapenaeus dalli Nauplius VI (a) ventral view; (b) first antenna; (c) second antenna; (d) mandible. Scale bar = 0.1 mm.
Figure 4 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 4. Coenobita brevimanus, second zoea: (A) antennule; (B) antenna; (C) mandibles; (D) maxillule; (E) maxilla; (F) first maxilliped; (G) second maxilliped; (H) third maxilliped; (I) telson. Scale bars: 100 μm (A–C, F–I) or 50 μm (D, E).
Figure 1 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 1. Numbers of larvae of Coenobita brevimanus reared individually in the laboratory. (A) Brood 1, hatched on 31 July 2008; (B) brood 2, hatched on 13 July 2010. Z1–Z4, first to fourth zoeae; MG, megalopa. Five specimens of each zoeal and megalopal stage were sampled from each brood.
Figure 5 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 5. Coenobita brevimanus, third zoea: (A) antennule; (B) antenna; (C) mandibles; (D) maxillule; (E) maxilla; (F) first maxilliped; (G) second maxilliped; (H) third maxilliped; (I) telson. Scale bars: 100 μm (A–C, F–I) or 50 μm (D, E).
Figure 2 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 2. Coenobita brevimanus, whole animals. Dorsal view: (A) first zoea; (B) second zoea; (C) third zoea; (D) fourth zoea; (E) megalopa. Lateral view: (F) first zoea; (G) second zoea; (H) third zoea; (I) fourth zoea. Scale bar: 1.0 mm.
Figure 10 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 10. The proportions of Coenobita brevimanus carrying three different sizes of gastropod shells. The small (S) and large (L) gastropod shells were Littoraria undulata and the medium (M) size shell was Littorina brevicula.
Figure 3 in Larval development and emigration behaviour during sea-to-land transition of the land hermit crab Coenobita brevimanus Dana, 1852 (Crustacea: Decapoda: Anomura: Coenobitidae) under laboratory conditions
Figure 3. Coenobita brevimanus, first zoea: (A) antennule; (B) antenna; (C) mandibles; (D) maxillule; (E) maxilla; (F) first maxilliped; (G) second maxilliped; (H) third maxilliped; (I) telson. Scale bars: 100 μm (A–C, F, G, I), or 50 μm (D, E, H).
FIGURES 23–28 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 23–28. Microphotographs of mature larvae of Euglossa (Euglossa) hemichlora. 23, 24. Right mandible, dorsal and inner views, respectively. 25. Anal area of cleared and stained predefecating larva with darkly stained integument above transverse anal slit above which is the pygidial ridge; dark area below slit is rectum seen through integument. Note short pigmented spines laterad of anal slit. 26. Spiracle of cleared predefecating larva seen from exterior, demonstrating width of peritreme relative to atrial opening. 27. Side view of spiracle of predefecating larva with its anatomical parts identified. 28. External view of spiracle of predefecating larva focused on unadorned primary tracheal opening; gray-toned ring immediately outside of opening caused by subatrium beneath transparent atrium.
FIGURES 15, 16 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 15, 16. SEM micrographs of egg of Euglossa (Euglossa) hemichlora. 15. Surface of chorion from side showing faint hexagonal patterning. 16. Presumed front end of egg revealing presumed opening through the vitelline membrane.
FIGURES 17–22 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 17–22. Diagrams of immature stages of Euglossa (Euglossa) hemichlora: figs. 18, 21, 22 to same scale. 17. Egg, presumed front end left. 18. Postdefecating larva, lateral view, anterior end left. 19, 20. Head of same, frontal and lateral views, respectively. 21. Late stage predefecating last larval instar, anterior end left. 22. Early stage of predefecating last larval instar, anterior end left.
FIGURES 11–14. Euglossine taxa not exhibiting elongate, necklike prothoracic segment. 11 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 11–14. Euglossine taxa not exhibiting elongate, necklike prothoracic segment. 11. Eulaema (Apeulaema) nigrita modified from Zucchi et al. (1969a: fig. 8) and 12, 13. Eufriesea surinamensis from Rozen (2016: figs. 6, 7) (both of which were identified as predefecating). 14. Exaerete smaragdina, identified as postdefecating in Garófalo and Rozen (2001: fig. 28), presumably was in an early stage that had not yet started to develop pupal tissue internally.
FIGURES 6–10 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 6–10. Euglossine taxa exhibiting elongate, necklike prothoracic segment and small cranium in Rozen (2016) for: 6, 7. Eufriesea surinamensis, 8. Eufriesea mussitans, 9. E. (Euglossa) cordata, 10. Eulaema (Apeulaema) polychroma.
FIGURES 3–5 in On Egg Eclosion and Larval Development in Euglossine Bees
FIGURES 3–5. Illustrations of sequence of immature stages of Euglossa (Glossura) intersecta Latreille reproduced from Zucchi et al. (1969b: figs. 3, 10, and 9, respectively), all to approximately same scale. 3. Postdefecating larva showing internal position of developing pharate pupa (dashed lines). 4. Prepupa. 5. Pupa, showing elongate mouthparts that form under the head and mesosoma of the prepupa.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.