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10 results for “leaf microbiome”
Reproductive hormones mediate changes in the gut microbiome during pregnancy and lactation in Phayre's leaf monkeys
Studies in multiple host species have shown that gut microbial diversity and composition change during pregnancy and lactation. However, the specific mechanisms underlying these shifts are not well understood. Here, we use longitudinal data from wild Phayre's leaf monkeys to test the hypothesis that fluctuations in reproductive hormone concentrations contribute to gut microbial shifts during pregnancy. We described the microbial taxonomic composition of 91 fecal samples from 15 females (n=16 cycling, n=36 pregnant, n=39 lactating) using 16S rRNA gene amplicon sequencing and assessed whether the resulting data were better explained by overall reproductive stage or by fecal estrogen (fE) and progesterone (fP) concentrations. Our results indicate that while overall reproductive stage affected gut microbiome composition, the observed patterns were driven by reproductive hormones. Females had lower gut microbial diversity during pregnancy and fP concentration was negatively correlated with diversity. Additionally, fP concentration predicted both unweighted and weighted UniFrac distances, while reproductive state only predicted unweighted UniFrac distances. Seasonality (rainfall and periods of phytoprogestin consumption) additionally influenced gut microbial diversity and composition. Our results indicate that reproductive hormones, specifically progestagens, contribute to the shifts in the gut microbiome during pregnancy and lactation.
Data from: Analysis of leaf microbiome composition of near-isogenic maize lines differing in broad-spectrum disease resistance
<p>Data and code associated with the submitted manuscript "Analysis of leaf microbiome composition of near-isogenic maize lines differing in broad-spectrum disease resistance". Detailed descriptions of each file can be found in the README.txt . The raw sequence data associated with this work can be downloaded from the NCBI Sequence Read Archive, listed under BioProject #PRJNA565009<strong>.</strong></p>
Reproductive hormones mediate changes in the gut microbiome during pregnancy and lactation in Phayre’s leaf monkeys
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Data from: Heterosis of leaf and rhizosphere microbiomes in field-grown maize
<p>Data and code associated with the submitted manuscript "<strong>Heterosis of leaf and rhizosphere microbiomes in field-grown maize</strong>". Detailed descriptions of each file can be found in the README.txt . The raw sequence data associated with this work can be downloaded from the NCBI Sequence Read Archive, listed under BioProject #PRJNA597058.</p>
Data from: Host genotype and age shape the leaf and root microbiomes of a wild perennial plant
Bacteria living on and in leaves and roots influence many aspects of plant health, so the extent of a plant's genetic control over its microbiota is of great interest to crop breeders and evolutionary biologists. Laboratory-based studies, because they poorly simulate true environmental heterogeneity, may misestimate or totally miss the influence of certain host genes on the microbiome. Here we report a large-scale field experiment to disentangle the effects of genotype, environment, age and year of harvest on bacterial communities associated with leaves and roots of Boechera stricta (Brassicaceae), a perennial wild mustard. Host genetic control of the microbiome is evident in leaves but not roots, and varies substantially among sites. Microbiome composition also shifts as plants age. Furthermore, a large proportion of leaf bacterial groups are shared with roots, suggesting inoculation from soil. Our results demonstrate how genotype-by-environment interactions contribute to the complexity of microbiome assembly in natural environments.
Data from: Host genotype and age shape the leaf and root microbiomes of a wild perennial plant
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Data from: Microbial transfer through fecal strings on eggs affect leaf beetle microbiome dynamics
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Data from: Insect herbivory reshapes a native leaf microbiome
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Structure vs. chemistry: Alternate mechanisms for controlling leaf microbiomes
<p><strong>Background</strong></p> <p>The analysis of phyllosphere microbiomes traditionally relied on DNA extracted from whole leaves. To investigate the microbial communities on the adaxial (upper) and abaxial (lower) leaf surfaces, swabs were collected from both surfaces of two garden plants, <em>Rhapis excelsa</em> and <em>Cordyline fruticosa</em>.</p> <p><strong>Results</strong></p> <p>The abaxial surface of <em>Rhapis excelsa</em> and <em>Cordyline fruticosa </em>had fewer bacteria in contrast to their adaxial counterpart. This observation was consistent between noon and midnight and across five different locations. Co-occurrence network analysis further showed that bacteria are found almost exclusively on the adaxial surface while only a small group of leaf blotch fungi survive on the abaxial surface. There are higher densities of stomata on the abaxial surface and these openings are vulnerable ports of entry into the plant host. While one might argue about the settling of dust particles and microorganisms on the adaxial surface, we detected differences in reactive chemical activities and microstructures between the adaxial and abaxial surfaces. Our results further suggest that both plant species deploy different defence strategies to deter invading pathogens on the abaxial surface.</p> <p><strong>Conclusions</strong></p> <p>We hypothesize that chemical and mechanical defence strategies evolved independently for harnessing and controlling phyllosphere microbiomes. Our findings have also advanced our understanding that the abaxial leaf surface is distinct from the adaxial surface and that the reduced microbial diversity is likely a consequence of plant-microbe interactions.</p>
Structure vs. chemistry: Alternate mechanisms for controlling leaf microbiomes
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.