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182 results for “leishmania”
Diffraction images used to solve the structures published in the article "A Family of Dual-Activity Glycosyltransferase-Phosphorylases Mediates Mannogen Turnover and Virulence in Leishmania Parasites"
<p>Raw diffraction images used for generating the structures published in the article A Family of Dual-Activity Glycosyltransferase-Phosphorylases Mediates Mannogen Turnover and Virulence in Leishmania Parasites" (available <a href="https://doi.org/10.1016/j.chom.2019.08.009">here</a>). The software used for the processing of each dataset is listed in their respective PDB entries.</p> <p> </p> <p>If you find this useful, please contact me at <a href="mailto:lukasz.sobala@hirszfeld.pl">lukasz.sobala@hirszfeld.pl</a>, I am just interested in how these data are used!</p>
Gene tagging and gene deletion resources for Leishmania mexicana MNYC/BZ/62/M379 Cas9/T7 strain
<p><em>primers_barcodes.csv</em>: List of primer sequences necessary for N and C terminus gene tagging, as well as gene deletion in the Leishmania mexicana MNYC/BZ/62/M379 Cas9/T7 strain. Each row contains the gene name and the DF (downstream forward), DR (downstream reverse), DSG (downstream guide sRNA), UF (upstream forward), UFB (upstream forward including a gene-unique 17nt barcode sequence), UR (upstream reverse), USG (upstream guide sRNA), VF (verification forward) and VR (verification reverse) primer sequences. Empty cells indicate that it was not possible to design this primer for this gene. Guide sRNA perfect match and off-target counts are included as well. The primer sequences were designed using LeishGEdit (http://www.leishgedit.net). Barcode sequences and assigned IDs for the unique identification of knock-out or tagged strains are included as separate columns. For recommended methods for endogenous tagging or gene deletion see Beneke <em>et al., </em>R. Soc. Open Sci.4170095 (2017), for generating barcoded deletion mutants see Beneke and Gluenz, Mol. Biochem. Parasitol. 239 (2020).</p> <p><em>genome.gff</em>: Annotated genome of the <em>L. mexicana</em> MNYC/BZ/62/M379 strain, genetically modified to express T7 RNA polymerase and Cas9. The annotation is provided in a combined GFF3 / FASTA format that also includes the sequences of the chromosomes and small contigs. The annotation also specifies polyadenylation sites (PAS features) and splice leader acceptor sites (SLAS features) which were used to refine the boundaries of protein-coding sequences as well as 3' and 5' untranslated regions over the reference genome of <em>L. mexicana</em> MNYC/BZ/62/M379<em>.</em></p> <p><em>c9t7_sequences.fasta</em>: Raw chromosome and contig sequences in FASTA format.</p> <p><em>c9t7_transcripts.fasta</em>: mRNA transcript sequences in FASTA format (includes 5' and 3' UTRs).</p> <p><em>c9t7_transcript_CDSs.fasta</em>: Coding sequences in FASTA format.</p> <p><em>c9t7_predicted_protein_sequences.fasta</em>: Predicted protein amino acid sequences in FASTA format.</p> <p>Note: This version provides an update for <em>c9t7_transcript_CDSs.fasta, c9t7_predicted_protein_sequences.fasta</em> and <em>genome.gff</em>, correcting an off-by-one sequence coordinate in 48 of the the protein-coding genes.</p>
Supplementary Figures. "In silico research of new therapeutics rotenoids derivatives against Leishmania amazonensis infection"
<p>Supplementary figures corresponding to the submitted manuscript entitled "In silico research of new therapeutics rotenoids derivatives against Leishmania amazonensis infection"</p>
High genome plasticity and frequent genetic exchange in Leishmania tropica isolates from Afghanistan, Iran and Syria
<p>Fastqs files for paper: "High genome plasticity and frequent genetic exchange in Leishmania tropica isolates from Afghanistan, Iran and Syria"</p>
Fig. 2 in Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA
Fig. 2 Phylogenetic tree demonstrating the relative position of Leishmania sp. strain 218-L139 based on a concatenated sequence comprising several nuclear loci. Phylogenetic tree demonstrating the relative position of Leishmania strain 218-L139 (brown star) based on alignment of a concatenated sequence comprising the 18S rDNA, 28S rDNA, CKIIα, GAPDH, and RPOIILS nuclear sequences. The alignment contains 6577 positions from 33 taxa. The tree was built using maximum likelihood (1000 bootstrap replicates) and Bayesian
Fig. 1 in Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA
Fig. 1 Morphology of Leishmania strain 218-L139 promastigotes and amastigotes. Cultured Leishmania strain 218-L139 promastigotes as observed under DIC microscopy following growth in complete RPMI culture medium without gentamicin (A). Giemsa-stained touch preparation made from a cutaneous lesion, showing Leishmania strain 218-L139 amastigotes inside a mononuclear phagocyte in addition to free amastigotes (B). Giemsastained smear of cultured promastigotes of Leishmania strain 218-L139 grown in gentamicin-free complete RPMI medium (C)
Fig. 3 in Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA
Fig. 3 Phylogenetic tree demonstrating the relative position of Leishmania strain 218-L139 based on partial maxicircle genome sequences. Phylogenetic tree demonstrating the relative position of Leishmania strain 218-L139 (brown star) based on alignment of partial maxicircle genome sequences. The alignment contains 9471 positions from 67 taxa. The tree was built using maximum likelihood (1000 bootstrap replicates) and the TVM + F + I + G4 model, and Bayesian inference using the GTR + I + G model where the proportion of invariable sites was set to 0.1. Posterior probability values and bootstrap support are reported for each node (pp/bs). The tree was rooted with the Strigomonas/Blechomonas/Herpetomonas clade. The relative position of each Leishmania subgenus is shown to the right of the corresponding clades (Leishmania, Sauroleishmania, Viannia, Mundinia). The scale bar represents the number of substitutions per site
Fig. 4 in The relationships among Leishmania infantum and phyllostomid bats assessed by histopathological and molecular assays
Fig. 4. Photomicrography of spleen of an adult male Artibeus planirostris (A) and Carollia perspicillata (B) qPCR Leishmania infantum positive. No amastigotes forms were found. Note tingible bodies macrophages in the germinal center containing phagocytic apoptotic cells (arrow) and apoptotic cells (arrowhead), H&E, 40x objective.
Fig. 2 in The relationships among Leishmania infantum and phyllostomid bats assessed by histopathological and molecular assays
Fig. 2. Photomicrography of liver of an adult female Leishmania infantum negative Carollia perspicillata presenting cytoplasmic vacuolation of hepatocytes (arrowhead) and mild lymphocytic infiltrate of portal area (arrow), H&E, 40x objective.
Fig. 3 in The relationships among Leishmania infantum and phyllostomid bats assessed by histopathological and molecular assays
Fig. 3. Photomicrography of spleen of an adult male Artibeus planirostris qPCR Leishmania infantum positive. No amastigotes forms were found, only unspecific mild hyperplasia (big ellipse) and hypoplasia (small ellipse) of lymphoid follicles in the reactive white pulp. Note the lack of delimitation between the WP and red pulp (RP), H&E, 10x objective. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in The relationships among Leishmania infantum and phyllostomid bats assessed by histopathological and molecular assays
Fig. 1. Photomicrography of wing skin of an adult male Artibeus planirostris qPCR Leishmania infantum positive. No amastigotes forms were found, only unspecific mild mixed inflammatory infiltrate of dermis with mononuclear (arrow) and polymorphonuclear cells (arrowhead), H&E, 40x objective.
Fig. 1 in Host competence of African rodents Arvicanthis neumanni, A. niloticus and Mastomys natalensis for Leishmania major
Fig. 1. Xenodiagnosis and external manifestation of L. major in rodents. Direct xenodiagnosis with P. duboscqi in plastic tubes covered with fine mesh held on the ear of the anaesthetized A. niloticus (A) and external manifestation of L. major LV109 in ear pinnae (site of inoculation) of A. neumanni by week 10 p.i., (B); A. niloticus by week 30 p.i. (C, D) and M. natalensis by week 19 p.i. (E).
Fig. 4 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
Fig. 4. Path analysis on the influences of contact and feeding on wild mammals in relation to infections of dogs surveyed at Urucum settlement, Corumbá, Mato Grosso do Sul, Brazil in 2015.
Fig. 3 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
Fig. 3. Path analysis on the influences of infections in relation to physical examination of dogs surveyed at Urucum settlement, Corumbá, Mato Grosso do Sul, Brazil in 2015.
Fig. 2 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
Fig. 2. Three-way Venn diagram illustrating coinfection, single infection or no infection of T. cruzi, T. evansi, and Leishmania spp. in 62 dogs from the Urucum settlement along the Brazil-Bolivia border. Total numbers and percentages are presented.
Fig. 1 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
Fig. 1. The Brazil-Bolivian border and Urucum settlement (Corumbá, MS) demonstrating the site of collections.
Figure 3 in Ecological aspects and molecular detection of Leishmania DNA (Kinetoplastida: Trypanosomatidae) in phlebotomine sand flies (Diptera: Psychodidae) from a rural settlement in the Eastern Amazon, Brazil
Figure 3 Abundance of Phlebotominae Sand flies from Perimetral Norte Rural Settlement, Pedra Branca Municipality, Amapá State, Brazil, collected from February 2018 to February 2019, at the collection sites: ID intradomicile, PD peridomicile, F100m forest 100m from edge, F400m forest from edge. The letters "a" and "b" represent the significant difference.
Figure 2 in Ecological aspects and molecular detection of Leishmania DNA (Kinetoplastida: Trypanosomatidae) in phlebotomine sand flies (Diptera: Psychodidae) from a rural settlement in the Eastern Amazon, Brazil
Figure 2 Shannon's Index of Phlebotominae Sand Flies from Perimetral Norte Rural Settlement, Pedra Branca Municipality, Amapá State, Brazil, collected from February 2018 to February 2019, at the collection- sites: ID intradomicile, PD peridomicile, F100m forest 100m from edge, F400m forest from edge. The letters "a" and "b" represent the significant difference.
Fig. 2 in Leishmania presence in bats in areas endemic for leishmaniasis in centralwest Brazil
Fig. 2. Agarose gel demonstrating kDNA PCR products (120bp). M: 100bp marker. 1–17: analyzed samples, of which 2–11, 13 and 16 were considered positive. PC: positive control of Leishmania spp., NC: negative control.
Fig. 2 in Molecular detection and characterization of Leishmania infantum in free- ranging Egyptian mongoose (Herpestes ichneumon)
Fig. 2. Maximum Likelihood (ML) phylogenetic tree of 27 L. infantum nucleotide sequences (410 nt long in the final dataset, including gaps), obtained during this study (MH799321) and others available in GenBank, based on the Hasegawa-Kishino-Yano model (HKY) (Hasegawa et al., 1985). The tree with the highest log likelihood (−1026.87) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 0.3289) (HKY + G). The tree was drawn to scale, with branch lengths measured in the number of substitutions per site. Robustness of the tree nodes was assessed by bootstrapping 1000 times. The graphical edition of the phylogenetic tree was performed with tree explorer, MEGA7 software (Kumar et al., 2016). Only bootstrap (BS) values equal or greater than 70 are shown on the tree, with the exception of the MH799321 cluster wherein the Bs values, although <70, are displayed for the reader. A 2-letter code and a specific colour (Top left) was attributed to each country for better identification of the origin of each strain. Whenever possible, the host was identified by a specific shape (Top left), namely dog (Canis lupus familiaris, star), human (Homo sapiens, triangle), Egyptian mongoose (Herpestes ichneumon, square) and hoary fox (Lycalopex vetulus, diamond). Sampling dates are indicated, whenever available. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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OpenNeuro
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