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482 results for “leukocyte”
Dataset for: Smoking does not accelerate leukocyte telomere attrition: a meta-analysis of 18 longitudinal cohorts
<p>Summary dataset (.csv file) and R script (.R file) for the manuscript entitled:</p> <p>Smoking does not accelerate leukocyte telomere attrition: a meta-analysis of 18 longitudinal cohorts.</p> <p>The column names are explained at the beginning of the R script.</p> <p> </p>
Fig. 4. Maximum Likelihood phylogenetic tree generated using N in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Fig. 4. Maximum Likelihood phylogenetic tree generated using N-terminal sequences of T. sp. (buffalo) and T. parva PIM antigen genes. Maximum composite likelihood trees were constructed using 1000 bootstrap replicates as implemented in MEGA5; the optimal nucleotide substitution model was identified using data monkey. The tree constructed with RAxML (Stamatakis et al., 2014) using a GTR/G/I model with 100 bootstrap iterations.
Fig. 5 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Fig. 5. Maximum Likelihood Phylogenetic trees illustrating the genetic relationships of T. parva CD8 T target antigen gene orthologues from T. sp. (buffalo). Panel (A) Tp6; Panel B Tp7: Panel C Tp8. Sequences were aligned and used to construct a maximum likelihood tree, at which the nodes were confirmed using 1000 bootstrap replications. The bootstrap values indicating the degree of support for each node are shown and also the GenBank accession numbers of the sequences. For Tp6, the tree was rooted using the prohibitin gene sequences present in Babesia bovis (XM001609045) and Theileria orientalis (AB161472). For Tp7, the tree was rooted using the putative Heat shock protein 90 gene sequences from Toxoplasma gondii (AY344115), Babesia bovis (AK442026) and Theileria annulata (XM_947380). For Tp8, the tree was rooted using an orthologue of Tp8 found in Theileria equi (CP001669).
Fig. 3 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Fig. 3. PCR amplification of genes encoding Theileria parva antigens from Marula schizont-infected leukocyte cultures. Panel A, p104 primers; Panel B PIM, primers; Panel C p67 primers. The order of the schizont-infected lymphocyte samples is (1) N6; (2). N13; (3). N18; (4). N20; (5). N33; (6). N36; (7). N38; (8). N43; (9). N50; (10). N55; (11). N69; (12). N76; (13). N77, (14). N79; (15). N86, (16). N88; (17). N99; (18). N100; (19). N102; (20). N103; (21). N106; (22). N107.
Fig. 2 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Fig. 2. Results of a semi-nested PCR assay used to amplify 18S ribosomal subunit DNA using primers specific for T. parva and T. sp. (buffalo). Samples are as follows: 1)N13 2)N18 3) N20 4)N33 5)N36 6) N43 7)N50 8)N55 9) N69 10)N76 11) N79 12) N86 13) N88 14) N99 15)N100 16) N102 17) N103 18)N107 19—21) T. parva clones 22—24) T. sp. (buffalo) clones (documented in Table 2).
Fig. 1 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Fig. 1. Reverse line blot analysis of schizont cultures containing parasites isolated from Marula farm. The following species-specific oligonucleotide probes were used (a) T. annulata, (b) T. parva, (c) T. mutans, (d) T. velifera, (e) T. taurotragi, (f) T. buffeli, (g) T. sp. (buffalo). (h) B. bigemina, (i) B. bovis. The order of the experimental samples hybridized is DNA from cell culture isolates in lanes 1—22 was lane 1; (1) N6, (2) N13, (3) N18, (4) N20, (5) N33, (6) N36, (7) N38 (8) N43, (9) N50 (10) N55, (11) N69, (12) N76, (13) N77, (14) N79, (15) N88, (16) N99, (17) N100 (18) N103, (19) N106, (20) N107, (21) N86, (22) N102 and DNA extracted from whole cattle blood (23) N106 (24) N69 (25) N86.
FIGURE 4 in Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal
FIGURE 4 | Eosinophil and basophil in Pygocentrus nattereri from Brazilian Pantanal in May Grunwald-Giemsa-Wright staining. A. eosinophil showing irregular nucleus, loose chromatin and specific cytoplasmic granules. B. basophil surrounded by erythrocytes presenting cytoplasm filled with basophilic granulations. Scale bars = 10 µm.
FIGURE 1 in Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal
FIGURE 1 | Curve fitting between standard-length (cm) and weight (g) in Pygocentrus nattereri. Age cut off (16 cm) was applied according to Lowe-McConnell (1964).
FIGURE 3 in Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal
FIGURE 3 | Neutrophil and PAS-positive granular leukocytes in Pygocentrus nattereri from Brazilian Pantanal in light (A and D) and transmission electronic microscopy (B, C, E, F). A–C. Neutrophil with a lumpy nucleus and cytoplasmic vacuoles showing fine azurophilic and neutrophilic granulations. B. Mitochondria (m) and an extensive network of cell synthesis organelles are also observed; nucleus (n); C, vacuoles (v) and granules with varying size and electron density are observed. D–F. PAS-positive granular leukocyte with a displaced euchromatic nucleus; cytoplasm rich in small vacuoles and azurophilic granulation. In E and F, the granules content electrolytic material is observed (g), possibly with internal electrodense areas in an elongated half-moon shape (arrows). Scale bars = A and D = 10 µm (May Grunwald-Giemsa-Wright staining), B and E = 2 µm; C and F = 500 nm.
FIGURE 2 in Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal
FIGURE 2 | Erythrocytes, thrombocytes and lymphocyte in Pygocentrus nattereri from Brazilian Pantanal in light (A, B, C) and transmission electronic microscopy (D, E, F). A and D. Thrombocytes of elongated shape and scarce cytoplasm rich in canalicular system (arrow). B and E, monocytes of irregular shape, chamfered nucleus, abundant cytoplasm mitochondria (m), vacuoles (v), glycogen granules (g), Golgi apparatus (G), and free ribosomes (r) are also observed. C and F. Small lymphocyte with numerous projections, a large nucleus, and scarce cytoplasm with few mitochondria and organelles. ABC. May Grunwald-Giemsa-Wright stain. Scale bars = A–C = 10 µm; D–F = 2 µm.
ADAM17 protects against elastase-induced emphysema by suppressing CD62L+ leukocyte infiltration in mice
<p>Pulmonary emphysema is a major manifestation of chronic obstructive pulmonary disease and is associated with chronic pulmonary inflammation caused by cigarette smoking, with contributions from immune cells such as neutrophils, macrophages, and lymphocytes. Although matrix metalloproteinases are well-known to contribute to emphysema progression, the role of a disintegrin and metalloproteinase (ADAM) family proteins, other major metalloproteinases, in disease pathogenesis is largely unknown. ADAM17 is a major sheddase that cleaves various cell surface proteins, including CD62L, an adhesion molecule that plays a critical role in promoting the migration of immune cells to the site of inflammation. In the present study, we aimed to investigate the potential role of ADAM17 and CD62L in the development of elastase-induced emphysema. Eight-to-ten-week-old control and <i>Adam17<sup>flox/flox</sup>/Mx1-Cre</i> (<i>Adam17<sup>ΔMx1</sup></i>) mice were intratracheally injected with 5 U of porcine pancreas elastase and monitored for 35 days after injection. Lung alveolar destruction was evaluated by analyzing the mean linear intercepts of lung tissue specimens and by histopathological examination. Mean linear intercepts data indicated that the degree of elastase-induced emphysema was significantly more severe in <i>Adam17<sup>ΔMx1</sup></i> mice. Further, flow cytometry showed that CD62L<sup>+</sup> neutrophil, CD62L<sup>+</sup> macrophage, and CD62L<sup>+ </sup>B lymphocyte numbers were significantly increased in <i>Adam17<sup>ΔMx1</sup></i> mice. Moreover, the pharmacological depletion of CD62L<sup>+</sup> cells with a CD62L-neutralizing antibody ameliorated the extent of emphysema in <i>Adam17<sup>ΔMx1</sup></i> mice. Collectively, these results suggest that ADAM17 possibly suppresses the progression of emphysema by proteolytically processing CD62L in immune cells and that ADAM17 and CD62L could be novel therapeutic targets for treating pulmonary emphysema.</p>
Low methylation windows from IHEC Healthy Leukocyte samples
<p>Data associated with low methylation windows from Healthy Leukocyte samples obtained from the International Human Epigenome Consortium (IHEC)</p>
An endothelial monolayer with leukocytes annotated for classification with bounding boxes for YOLO
<p>Movies of leukocyte TEM generated by Max Grönloh (Sanquin Research) were used as input for deep learning analysis with YOLO on the ZeroCostDL4Mic platform.</p> <p>Bounding boxes generated with Makesense.ai by Guusje Mouton.</p> <p>After training, an unseen dataset was analysed. For results see: 'Filmpje_Predictions_YOLO.gif'</p> <p>The result shows a large number of false negatives, which can potentially be improved by increasing the volume of training data.</p>
Data for: Endo-lysosomal assembly variations among Human Leukocyte Antigen class I (HLA-I) allotypes
<p>The extreme polymorphisms of HLA-I proteins enable the presentation of diverse peptides to cytotoxic T lymphocytes (CTL). The canonical endoplasmic reticulum (ER) HLA-I assembly pathway enables presentation of cytosolic peptides, but effective intracellular surveillance requires multi-compartmental antigen sampling. Endo-lysosomes are generally sites of HLA class II assembly, but human monocytes and monocyte-derived dendritic cells (moDCs) also contain significant reserves of endo-lysosomal HLA-I molecules. We hypothesized variable influences of HLA-I polymorphisms upon outcomes of endo-lysosomal trafficking, as the stabilities and peptide occupancies of cell surface HLA-I are variable. Consistent with this model, when the endo-lysosomal pH of moDCs is disrupted, HLA-B allotypes display varying propensities for reductions in surface expression, with HLA-B*08:01 or HLA-B*35:01 being among the most resistant or sensitive respectively, among eight tested HLA-B allotypes. Perturbations of moDC endo-lysosomal pH result in redistribution of HLA-B*35:01, but not HLA-B*08:01, to LAMP1+ compartments and increase HLA-B*35:01 peptide receptivity. These findings reveal the intersection of the vacuolar cross-presentation pathway with a constitutive assembly pathway for some HLA-B allotypes. Notably, cross-presentation of epitopes derived from two soluble antigens was also more efficient for B*35:01 compared to B*08:01, even when matched for T cell response sensitivity, and more affected by cathepsin inhibition. Thus, HLA-I polymorphisms dictate the degree of endo-lysosomal assembly, which can supplement ER assembly for constitutive HLA-I expression and increase the efficiency of cross-presentation.</p>
Comparison of Tacrolimus Extended-Release (Envarsus XR) to Tacrolimus Immediate-Release in Human Leukocyte Antigen (HLA) Sensitized Kidney Transplant Recipients
ClinicalTrials.gov study NCT04225988. IPD Sharing: NO. Countries: 1. Publications: 9.
Effects of Mycophenolate Mofetil (MMF) On Anti-HLA (Human Leukocyte Antigen)Antibody Levels In Patients Awaiting Cadaveric Renal Transplant.
ClinicalTrials.gov study NCT00446459. IPD Sharing: Not stated. Countries: 1. Publications: 9.
Leukocyte Function in Chronic Obstructive Pulmonary Disease (COPD)
ClinicalTrials.gov study NCT00147082. IPD Sharing: NO. Countries: 1. Publications: 1.
Efficacy and Safety Study of Leukocyte Interleukin,Injection (LI) to Treat Cancer of the Oral Cavity
ClinicalTrials.gov study NCT01265849. IPD Sharing: NO. Countries: 23. Publications: 1.
Effects of Orally Administered Beta-glucan on Leukocyte Function in Humans
ClinicalTrials.gov study NCT01727895. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Human Leukocyte Antigen-A*02:01-restricted Tumor Vessel Specific Peptide Vaccination for Advanced Pancreatic Cancer
ClinicalTrials.gov study NCT00683085. IPD Sharing: Not stated. Countries: 1. Publications: 2.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.