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opencc-by-4.0Apr 2020View details →

Light micrographs on the morphological response to heat stress in the filamentous Zygnematophyceae Mougeotia sp. and Spirogyra pratensis

Herein are the light micrographs of Mougeotia sp. and Spirogyra pratensis that were used to obtain the quantitative information for Figure 1 in the article "Heat stress response in the closest algal relatives of land plants reveals conserved stress signaling circuits" published in The Plant Journal, doi: 10.1111/tpj.14782

opencc-by-4.0Apr 2024View details →

Supplementary Materials of "Elementary mathematics helps to shed light on the transpiration budget under water stress"

This directory contains the Jupyter notebook used to do complete analysis from our paper "Elementary mathematics sheds light on the transpiration budget under water stress" submitted to the Ecohydrology Journal at the Special Issues "ECOHYDROLOGY OF INLAND AND COASTAL WATERS in honor of Ignacio Rodriguez-Iturbe". These materials are referenced in the main text and supplemental text of the publication. The purpose of this repository is to facilitate replication of our analysis by any interested parties.  Specifically, this directory contains ten files: <ul> <li>From 0 to 5, Jupyter Notebook prepared and used for the analysis (please execute the notebooks in numerical sequence). </li> <li>"Table_S1.xlsx" Data From: Kröber, W., H. Heklau, and H. Bruelheide. 2015. “Leaf Morphology of 40 Evergreen and Deciduous Broadleaved Subtropical Tree Species and Relationships to Functional Ecophysiological Traits.” Plant Biology  17 (2): 373–83. <a href="https://doi.org/10.1111/plb.12250">https://doi.org/10.1111/plb.12250</a>.</li> <li>"Richards_VG.csv" contains Van Genuchten Parameters for various soils.</li> <li>"The_Rosetta_Stone_of_the_Darcy_Buckingham_law.pdf" addresses the challenge of converting water flux units between Darcy-like soil and plant descriptions, where hydrologists use "head" units (meters) and plant physiologists use pressure potential (MPa). The aim is to clarify and perform the necessary unit conversions, with detailed explanations available in the relevant section on <a href="https://abouthydrology.blogspot.com/2022/10/my-water-management-in-agricolture.html">this webpage</a>.</li> </ul>

Fig. 6 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 6: Maxiumum growth rate determination of all temperatures, light intensities and nutrient concentrations.

Fig. 5 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 5: Entomoneis sp biomass (Chl a, µg /L) under different N/P ratios and light intensities (a) representing growth under T1°C (b) T2°C (c) and T3°C.

Fig. 2 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 2: 3D response surface plot and contour line of Box– Behnken Design showing the mutual effect of temperature and light intensity on chlorophyll a concentration (µg/L) of Entomoneis sp. using an N/P ratio of 11.

Fig. 3 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 3: 3D response surface plot and contour line of Box– Behnken Design showing the mutual effect of temperature and light intensity on growth rate (day-1) of Entomoneis sp. using an N/P ratio of 4.4.

Fig. 4 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 4: 3D response surface plot and contour line of Box– Behnken Design showing the mutual effect of temperature and light intensity on growth rate (day-1) of Entomoneis sp. using an N/P ratio of 27.

Fig. 4 in Effect of light stress on Crotalaria spectabilis (Fabaceae) and on its herbivore insect, the moth Utetheisa ornatrix (Erebidae: Arctiinae)

Fig. 4. Weight of the pupae of Utetheisa ornatrix (L., 1758) whose larvae were raised with leaves of Crotalaria spectabilis Roth from light stressed plants and non-stressed plants. (A) male pupae; N = 30 for stressed plants and N = 18 for non-stressed plants. (B) female pupae; N = 19 for stressed plants and N = 28 for non-stressed plants. Different letters indicate statistical difference (t = -2.7531; p = 0.009).

Fig. 5 in Effect of light stress on Crotalaria spectabilis (Fabaceae) and on its herbivore insect, the moth Utetheisa ornatrix (Erebidae: Arctiinae)

Fig. 5. FecunditY of Utetheisa ornatrix (L., 1758) females whose larvae were reared on stressed and non-stressed leaves of Crotalaria spectabilis Roth. N = 18 for stressed plants and N = 15 for non-stressed plants.

Fig. 3 in Effect of light stress on Crotalaria spectabilis (Fabaceae) and on its herbivore insect, the moth Utetheisa ornatrix (Erebidae: Arctiinae)

Fig. 3. Development time of the larvae of Utetheisa ornatrix (L., 1758) reared with leaves of Crotalaria spectabilis Roth from light stressed plants and non-stressed plants. N = 49 for stressed plants and N = 46 for non-stressed plants. Different letters indicate statistical difference (t=2.27; p=0.02).

Fig. 1 in Effect of light stress on Crotalaria spectabilis (Fabaceae) and on its herbivore insect, the moth Utetheisa ornatrix (Erebidae: Arctiinae)

Fig. 1. Distribution of stressed plants (with mesh cover) and non-stressed plants of Crotalaria spectabilis Roth in the greenhouse.

opencc-by-3.0Oct 2022View details →

Photophysiology and molecular responses of the soft coral Sarcophyton cf glaucum exposed to heat and high light stress

An experiment testing different temperatures (26 vs 32 ºC) and light intensities (high light HL and low light LL, &sim;662 and 253 µmol photons m-2 s-1) was carried out using the soft coral Sarcophyton cf glaucum (leather coral) as model species. In summary, corals were exposed to the different light intensities for 30 days (Photoacclimation, time-point 1) and a subsequent marine heatwave simulation was carried out for 10 days (Marine heatwave, time-point 2). Subsequently, corals were returned to control temperature and allowed to recover for 30 days (Recovery, time-point 3). Photophysiological performance (maximum quantum yield of photosystem II (Fv/Fm), a measure of photosynthetic activity; dark-level fluorescence (F0), as a proxy of chlorophyll a content; and zooxanthellae density) and stress biomarkers (total protein, catalase - CAT, superoxide dismutase - SOD, glutathione-S-transferase - GST, total antioxidant capacity - TAC, lipid peroxidation - LPO, ubiquitin - UBI, and heat shock protein 70 - Hsp70) were assessed in corals at these three time-points.

opencc-zeroDec 2019View details →

Data from: Early-life exposure to artificial light at night elevates physiological stress in free-living songbirds

Artificial light at night (ALAN) can disrupt adaptive patterns of physiology and behavior that promote high fitness, resulting in physiological stress and elevation of steroid glucocorticoids (corticosterone, CORT in birds). Elevated CORT may have particularly profound effects early in life, with the potential for enduring effects that persist into adulthood. Research on the consequences of early-life exposure to ALAN remains limited, especially outside of the laboratory, and whether light exposure affects CORT concentrations in wild nestling birds particularly remains to be elucidated. We used an experimental setup to test the hypothesis that ALAN elevates CORT concentrations in developing free-living birds, by exposing nestling great tits (Parus major) to ALAN inside nest boxes. We measured CORT in feathers grown over the timeframe of the experiment (7 nights), such that CORT concentrations represent an integrative metric of hormone release over the period of nocturnal light exposure, and of development. We also assessed the relationships between feather CORT concentrations, body condition, nestling size rank and fledging success. In addition, we evaluated the relationship between feather CORT concentrations and telomere length. Nestlings exposed to ALAN had higher feather CORT concentrations than control nestlings, and nestlings in poorer body condition and smaller brood members also had higher CORT. On the other hand, telomere length, fledging success, and recruitment rate were not significantly associated with light exposure or feather CORT concentrations. Results indicate that exposure to ALAN elevates CORT concentrations in nestlings, which may reflect physiological stress. In addition, the organizational effects of CORT are known to be substantial. Thus, despite the lack of an effect on telomere length and survivorship, elevated CORT concentrations in nestlings exposed to ALAN may have subsequent impacts on later-life fitness and stress sensitivity.

opencc-zeroJul 2019View details →

Data from: Varieties of the highly dispersible and hypervariable tree, Metrosideros polymorpha, differ in response to mechanical stress and light across a sharp ecotone

PREMISE OF THE STUDY: The drivers of isolation between sympatric populations of long-lived and highly-dispersible conspecific plants are not well understood. In the Hawaiian Islands the landscape-dominant tree, Metrosideros polymorpha, displays extraordinary phenotypic differences among sympatric varieties despite high dispersibility of its pollen and seeds, thereby presenting a unique opportunity to investigate how disruptive selection alone can maintain incipient forms. Stenophyllous M. polymorpha var. newellii is a recently evolved tree endemic to the waterways of east Hawai'i Island that shows striking neutral genetic differentiation from its ancestor, wet-forest var. glaberrima, despite sympatry of these forms. We looked for evidence for, and drivers of, differential local adaptation of these varieties across the range of var. newellii. METHODS: For paired populations of these varieties, we compared seedling performance under contrasting light conditions and a strong water current characteristic of the riparian zone. We also conducted a reciprocal transplant experiment and contrasted adult leaf anatomy. KEY RESULTS: Results suggest that the riparian zone is harsh and that selection involving the mechanical stress of rushing water, and secondarily light, lead to significant reciprocal immigrant inviability in adjacent forest and riparian environments. The strongest adaptive divergence between varieties was seen in leaves and seedlings from the site with the sharpest ecotone, coincident with the strongest genetic isolation of var. newellii observed previously. CONCLUSIONS: These findings suggest that disruptive selection across a sharp ecotone contributes to the maintenance of an incipient riparian ecotype from within a continuous population of a long-lived and highly dispersible tree species.

zenodo36/100

Additional raw video and pose estimation data of top view mouse behavior recordings (marble burying test, light-dark box, fear conditioning box) of acute and chronic stress models

This repository contains raw data for 296 different behavioral recordings of mice (marble burying test, light-dark box, fear conditioning box). These include top view raw video .mp4 files (Videos.zip) and the corresponding .csv pose estimation data (data.zip) obtained with DeepLabCut. The data is from multiple different experiments. The METADATA.csv or METADATA.xlsx files contain all grouping variables and help linking the pose estimation files (located in multiple subfolders of /data) to the video files. Visit https://github.com/ETHZ-INS/BehaviorFlow to find out more about how this data has be used by us.

opencc-by-4.0May 2024View details →

zenodo36/100

Fig. 1 in Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract

Fig. 1: Schematic diagram of methodology.

controlledIPD-YESFeb 2026View details →

ClinicalTrials.gov36/100

Morning Light Treatment for Traumatic Stress: The Role of Amygdala Reactivity

ClinicalTrials.gov study NCT04117347. IPD Sharing: YES. Countries: 1. Publications: 1.

publicJul 2019View details →

Data from: Varieties of the highly dispersible and hypervariable tree, Metrosideros polymorpha, differ in response to mechanical stress and light across a sharp ecotone

Open the record for dataset details and reuse information.