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23 results for “mCherry”
mCherry fluorescence correlation with western blot band intensity
<p><strong>Overview</strong></p> <p>The results present in this dataset were obtained from recombinant <em>Chlamydomonas reinhardtii</em> expressing the fluorescent protein mCherry in different constructs formats, aiming to recombinant protein secretion. </p> <p>Image present in this dataset were obtained by following the steps described in the protocol dx.doi.org/10.17504/protocols.io.kfpctmn. </p> <p>Fluorescence measurements were obtained by growing cc1690 in 50 mL TAP media on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m<sup>2</sup>s). Then, 100 μL of each strain was transferred to a clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland). Fluorescence was measured at excitation 575/9 nm and emission 608/20 nm. Supernatant samples were obtained by spinning 1 mL sample at 15000 × <em>g</em> for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement.</p> <p><strong>Files Info</strong></p> <p>021115_20171219 Correlation_Fluore_vs_Densito_mCherry_50mL_7_days.xlsx -> data points for fluorescence measurements and pixel intensity obtained with FiJI (Fiji Is Just ImageJ). </p> <p>022615 WB SP mCHerry Lysate 3.JPG -> Raw western blot image for lysate samples</p> <p>022615 WB SP mCHerry Supernatant 3 .JPG -> Raw western blot image for supernatant samples </p> <p>20171219 Fluor_Densito_Correlation_Lys.png -> Correlation plot of fluorescence and pixel intensity for lysate samples</p> <p>WB_Lysa_7days_for_Densitom.png -> Prepared image for densitometry analysis of lysate samples</p> <p>20171219 WB_Sup_7days_for_Densitom.png -> Correlation plot of fluorescence and pixel intensity for supernatant samples</p> <p>WB_Sup_7days_for_Densitom.png -> Prepared image for densitometry analysis of supernatant samples</p> <p> </p> <p><strong>Consider citing our work. </strong></p> <p>Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433<br> </p> <p> </p> <p> </p>
Confocal stacks of Cii_beta gamma crystalin_hM4D(Gi)_mCherry transgenic Ciona Larvae
<p>Confocal stacks of Cii_beta gamma crystalin_hM4D(Gi)_mCherry transgenic Ciona Larvae used to generate the panels of Hoyer et al.</p>
Fluorescence Microscopy Images of Hela Cell infected with Plasmodium Berghei parasite expressing mCherry in cytoplasm
<p>The purpose of our experiments was to delve into the liver stage development of the P. berghei parasite and examine the host-parasite interactions using HeLa cells. This research is primarily focused on in vitro analysis and does not extend to in vivo applications. Our study investigated the integration of fluorescent microscopy with artificial intelligence to <br>track and predict the developmental milestones of Plasmodium liver stage development. </p> <p>This is the dataset used in our study.</p>
SN2N's 3D dataset of outer mitochondrial membrane network of live COS-7 cells labeled with Tom20-mCherry on SD-SIM sysytem.
Open the record for dataset details and reuse information.
Fluorescence microscopy of Chlamydomonas reinhardtii for mCherry detection: secretion peptides strains.
<p><strong>Overview</strong></p> <p>Life-cell imaging was performed with a confocal fluorescence microscopy to observe mCherry in the secretion vacuoles. mCherry fluorescence compartmentalization was observed by a Confocal Zeiss LSM 780-NLO, using an argon laser 543 nm to excite mCherry and a spectral detector set approximately to 610-650 nm range. For chlorophyll, we used a laser at 405 nm for excitation, and spectral detector set to 680 nm region. All pictures were taken with the same system configuration and analyzed by Fiji, an ImageJ distribution software. Cells images were acquired in bundles of 0.4 μm afar photos per channel in the z-axis. </p> <p> </p> <p><strong>Files info:</strong></p> <p>Each file is the raw image obtained from fluorescent microscopy.</p> <p> </p> <p><strong>Organization</strong></p> <p>Construct_name.czi - Ex: "pAH04mCherry.czi"</p> <p>pAH04mCherry -> construct without signal peptide</p> <p>pJP22mCherry -> construct with signal peptide from arylsulfatase 1 (<em>Chlamydomonas </em><em>reinhardtii</em>)</p> <p>pJP26mCherry -> construct with signal peptide from binding protein 1 (<em>C. </em><em>reinhardtii</em>)</p> <p>pJP28mCherry -> construct with signal peptide from carbonic anhydrase 1 (<em>C. </em><em>reinhardtii</em>)</p> <p>pJP29 mCherry -> construct with signal peptide from ice-binding protein 1 (Artic <em>Chlamydomonas sp</em>)</p> <p>pJP30-35mCherry -> construct with signal peptide from in silico identified list (DOI 10.5281/zenodo.556792).</p> <p>Wildtype cc1690 -> parental strain used for transformation.</p> <p> </p> <p>For more information on the constructs, check our paper. </p> <p> </p> <p><strong>Consider citing our work. </strong></p> <p>Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433</p> <p> </p>
Confocal stacks of Cii_PC2_hM4D(Gi)_mCherry transgenic Ciona Larvae
<p>Data related to Supplementary Figure 5 of the manuscript titled: <strong>Polymodal sensory perception drives robust attachment and metamorphosis of a pre-vertebrate zooplanktonic larva. </strong></p>
Fig S4 mCherry controls, in Regulation of REM Sleep by Inhibitory Neurons in the Dorsomedial Medulla
<p>Sleep recordings for mCherry controls. Associated with Figure S4.</p>
Data for manuscript: PEG-mCherry interactions beyond classical macromolecular crowding
<p>The data is provided as a part of the manuscript "<strong>PEG-mCherry interactions beyond classical macromolecular crowding</strong>". </p> <p>This repository includes an archive with folders:<br><br><strong>MDData</strong></p> <ul> <li>contains simulations system: mCherry in PEG and mCherry in water built form the crystal structure of mCherry (PDB ID: 2H5Q) and carried out using CHARMM36m <em>The details of the simulations are provided in the Methods section of the manuscript. </em>Thinned trajectories, initial structures and tprs are provided.</li> </ul> <p><strong>FigureData</strong></p> <ul> <li>contains the simulation data sets underlying the figures where applicable</li> </ul> <p><strong>AdditionalFiles</strong></p> <ul> <li>Contains additional files for supporting data</li> </ul> <p><strong>ExperimentalData</strong></p> <ul> <li><strong>FCS</strong> <ul> <li>Contains data and analysis for Figures 7, S24, S26, and S27</li> </ul> </li> <li><strong>Lifetimes</strong> <ul> <li>Contains data and analysis for Figure 8</li> </ul> </li> <li><strong>Thermal Stability</strong> <ul> <li>Contains data and analysis for Figure S25</li> </ul> </li> <li><strong>PVA</strong> <ul> <li>Contains the lifetime data for PVA measurements (Figure 8)</li> </ul> </li> </ul>
Pot-1::mCherry transgene images from: Gametes deficient for Pot1 telomere binding proteins alter levels of telomeric foci for multiple generations
Open the record for dataset details and reuse information.
Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models
<p>Figures and open datasets from the publication about the reporter TBEV in tick experimental models by Kevély et al., 2022 (<a href="https://doi.org/10.3390/v14122673">https://doi.org/10.3390/v14122673</a>)</p>
ChIP-seq with Mucor circinelloides Mis12- and Dsn1-mCherry proteins
GEO Series GSE132687. Mucor lusitanicus. 5 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Gene expression signatures of MDCK-pTR GFP-RasV12 mCherry-β-catenin Δ131 cells cultured alone or mixed with MDCK mCherry-β-catenin Δ131 cells
GEO Series GSE217830. Canis lupus familiaris. 6 samples. Type: Expression profiling by array.
Expression data from isogenic Pten WT mouse T-ALLs infected with MSCV Myr-AKT-IRES-mCherry or empty vector, treated with DBZ or DMSO
GEO Series GSE71089. Mus musculus. 12 samples. Type: Expression profiling by array.
RNA-sequencing in mESCs with overexpression of mCherry-tagged DUX and DUX domain derivatives
GEO Series GSE224296. Mus musculus. 22 samples. Type: Expression profiling by high throughput sequencing.
Single cell profiling of CD11b+ cells from mouse cortex following astrocyte overexpression of mCherry-CAAX or Hevin
GEO Series GSE289516. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
GFP and mCherry gene expression at single cell level of all liver cell types from B6J mice
GEO Series GSE250338. Mus musculus. 1 samples. Type: Expression profiling by high throughput sequencing.
Comparative gene expression profiling analysis of RNA-seq data for mCherryHigh and DTC populations compared to mCherryLow metastatic populations [mCherry Pops and DTCs]
GEO Series GSE232197. Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Quiescent cancer cells resist T cell attack by forming an immunosuppressive niche (GFP:mCherry 1:1 4T07 mix tumors after Jedi killing Bulk RNA)
GEO Series GSE198712. Mus musculus. 16 samples. Type: Expression profiling by high throughput sequencing.
Detection of viral double-stranded RNA from Vero cells infected with HSV-1-mCherry
GEO Series GSE101960. Human alphaherpesvirus 1; Chlorocebus aethiops. 9 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profilling of NEUROG2-mCherry lineage in cerebral organoids
GEO Series GSE277092. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
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OpenNeuro
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