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1,405 results for “mRNA expression”
Arabidopsis thaliana circadian mRNA-seq gene expression processed tables from Romanowski et al., TPJ 2020.
<p>This dataset is an add-on for Romanowski et al., TPJ 2020 (https://doi.org/10.1111/tpj.14776) containing processed files for the circadian RNAseq data in tab delimited txt format.</p> <p><br> Here, you can the raw counts file, the normalized CPM values, and the full JTK result (without recalculated circadian phases, just the original ones). All genes with a read density > 0.05 in at least one timepoint were considered expressed. The read density is calculated as the amount of reads divided by the effective length of a gene (total reads / length). Genes rd file is also included.</p> <p>Some useful notes:<br> 1) Counts were assigned using ASpli and the AtRTDv2 annotation (34,212 genes).<br> 2) After filtering by rd we had a total of 18,503 expressed genes.<br> 3) 13,256 genes passed the QL F-tests.<br> 4) 9,127 genes were rhythmic according to JTK_cycle. </p> <p>For detailed protocols, please see Romanowski et al., TPJ 2020 (https://doi.org/10.1111/tpj.14776)</p> <p>The RNA-seq raw data supporting the conclusions of this article have been deposited in ArrayExpress (Kolesnikov et al., 2015) at EMBL-EBI (www.ebi.ac.uk/arrayexpress), under accession numbers E-MTAB-7933.</p> <p>All relevant custom r scripts are available at https://github.com/aromanowski/Circadian_rhythms_and_alternative_splicing</p>
CGGA mRNA-array_301 Expression Data
<p><strong>Abstract:</strong></p> <p>The Chinese Glioma Datasets (CGGA) are comprehensive and valuable collections of data related to glioma, a type of brain tumor, originating from Chinese patients. The CGGA is a data portal for the storage and interactive exploration of cross-omics data, including nearly 2000 primary and recurrent glioma samples. This dataset's gene expression profile was measured experimentally using Agilent-014850 Whole Human Genome Microarray. The Sample IDs serve as unique identifiers for each sample. </p> <p><strong>Inspiration:</strong></p> <p>This dataset was uploaded to UBRITE for GTKB project. </p> <p><strong>Instruction:</strong></p> <p>z-normalization was performed on all the samples </p> <p><strong>Acknowledgments:</strong></p> <p>Zhao, Z., Zhang, KN., Wang, QW., et al. Chinese Glioma Genome Atlas (CGGA): A Comprehensive Resource with Functional Genomic Data from Chinese Glioma Patients (2021). Genomics, Proteomics & Bioinformatics 19(1):1-12.</p> <p>Fang, S., Liang, J., Qian, T., et al. (2017). Anatomic Location of Tumor Predicts the Accuracy of Motor Function Localization in Diffuse Lower-Grade Gliomas Involving the Hand Knob Area. AMERICAN JOURNAL OF NEURORADIOLOGY. 38(10): 1990-1997</p> <p>Wang, Y., Wang, Y., Fan, X., et al. (2017). Putamen involvement and survival outcomes in patients with insular low-grade gliomas. JOURNAL OF NEUROSURGERY. 126(6): 1788-1794.</p> <p> </p> <p><strong>U-BRITE last update: </strong>07/27/2023</p>
mRNA expression data of genes related to mitochondrial quality control in hepatopancreas of the two marine bivalves, Mytilus edulis and Crassostrea gigas, during short-term hypoxia/reoxygenation stress
<p>Coastal environments commonly experience strong oxygen fluctuations. Resulting hypoxia/reoxygenation stress can negatively affect mitochondrial functions, since oxygen deficiency impairs ATP generation, whereas a surge of oxygen causes mitochondrial damage by oxidative stress mechanisms. Marine intertidal bivalves are adapted to fluctuating oxygen conditions, yet the underlying molecular mechanisms that sustain mitochondrial integrity and function during oxygen fluctuations are not yet well understood. We used targeted mRNA expression analysis to determine the potential involvement of the mitochondrial quality control mechanisms in responses to short-term hypoxia (24 h at <0.01% O<sub>2</sub>) and subsequent reoxygenation (1.5 h at 21% O<sub>2</sub>) in two hypoxia-tolerant marine bivalves, the Pacific oysters <em>Crassostrea gigas</em> and the blue mussels <em>Mytilus edulis</em>. To test these hypotheses, We focused on the transcript levels of the following marker genes: for mitochondrial fission and fusion - <em>mfn</em>2 (encoding mitofusin 2), <em>opa</em>1 (mitochondrial dynamin-like 120kDa protein), <em>dnm</em>1<em>l </em>(dynamin-1-like protein), <em>mff</em> (mitochondrial fission factor), <em>fis</em>1 (mitochondrial fission protein 1); for protein and DNA quality control - <em>tsfm</em> (encoding mitochondrial translation elongation factor Ts), <em>lonp</em>1 (mitochondrial Lon protease), <em>spg</em>7 (paraplegin), <em>oma</em>1 (mitochondrial metalloendopeptidase OMA1), <em>clpB</em> (mitochondrial caseinolytic matrix peptidase chaperone subunit B), <em>atp</em>23 (mitochondrial inner membrane protease ATP23), <em>twnk</em> (mitochondrial twinkle mtDNA helicase); and for mitophagy - <em>mieap</em> (encoding mitochondrial eating protein), <em>hyou</em>1 (hypoxia upregulated protein 1), <em>prkn</em> (parkin), <em>pink</em>1 (PTEN- induced kinase 1), and <em>pgam</em>5 (mitochondrial serine/threonine protein phosphatase PGAM5). The revealed species-specific differences in the expression of the mitochondrial quality control pathways shed light on the potentially important mechanisms of mitochondrial protection against H/R-induced damage that might contribute to hypoxia tolerance in marine bivalves. </p>
Effect of shade and nitrogen content on Arabidopsis Col-0 and cytokinin mutants abcg14 and cypDM mRNA-seq gene expression processed tables.
<p>This dataset is an add-on for Gautrat et al., containing processed files for the mRNAseq data in tab delimited txt format.</p> <p>Here, you can obtain the raw counts file, the normalized CPM values, and the normalized logCPM values</p> <p>The RNA-seq raw data supporting the conclusions of this article have been deposited in ArrayExpress (Kolesnikov et al., 2015) at EMBL-EBI (www.ebi.ac.uk/arrayexpress), under accession numbers E-MTAB-13638.</p> <p>All relevant custom r scripts are available at https://github.com/aromanowski/shade_N_ck</p>
Supplementing data and code for: "Correlation of mRNA delivery timing and protein expression in lipid-based transfection"
<p>Supplementary data and code for Reiser <em>et al.</em>: Correlation of mRNA delivery timing and protein expression in lipid-based transfection. 2019, <a href="https://doi.org/10.1093/intbio/zyz030">doi:10.1093/intbio/zyz030</a>.</p> <p>See `README.pdf` for further details.</p>
Trans-acting genotypes drive mRNA expression affecting metabolic and thermal tolerance traits
<p>Evolutionary processes driving physiological trait variation depend on the underlying genomic mechanisms. Evolution of these mechanisms depends on whether traits are genetically complex (involving many genes) and how gene expression that impacts the traits is converted to phenotype. Yet, genomic mechanisms that impact physiological traits are diverse and context-dependent (e.g., vary by environment or among tissues), making them difficult to discern. Here we examine the relationships between genotype, mRNA expression, and physiological traits to discern the genetic complexity and whether the gene expression affecting the physiological traits is primarily cis or trans-acting. We use low-coverage whole genome sequencing and tissue-specific mRNA expression among individuals to identify polymorphisms directly associated with physiological traits and expressed quantitative trait loci (eQTL) driving variation in six temperature-specific physiological traits (standard metabolic rate, thermal tolerance, and four substrate-specific cardiac metabolic rates). Not surprisingly, there were few, only five, SNPs directly associated with physiological traits. Yet, by focusing on a select set of mRNAs belonging to co-expression modules that explain up to 82% of temperature specific (12°C or 28°C) metabolism and thermal tolerance, we identified hundreds of significant eQTL for mRNA whose expression affects physiological traits. Surprisingly, most eQTL (97.4% for heart and 96.7% for brain) of eQTL were trans-acting. This could be due to higher effect size or greater importance of trans versus cis-acting eQTLs for mRNAs that are central to co-expression modules. That is, we may have enhanced the identification of trans-acting factors by looking for SNPs associated with mRNAs in co-expression modules that are known to be correlated with the expression of 10s or 100s of other genes, and thus have identified eQTLs with widespread effects on broad gene expression patterns. Overall, these data indicate that the genomic mechanism driving physiological variation across environments is driven by trans-acting tissue-specific mRNA expression.</p>
Trans-acting genotypes drive mRNA expression affecting metabolic and thermal tolerance traits
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The mRNA expression changes in neonatal primary mouse ventricular cardiomyocytes treated with epirubicin (2uM)
<p>Epirubicin (EPI) is effective in the treatment of malignant cancers, but its application is limited by life-threatening cardiotoxicity. Iron homeostasis disturbance has been implicated in anthracycline-induced cardiotoxicity (AIC), and ferroptosis is involved in AIC which is dependent upon intracellular iron. However, the role and exact mechanisms of ferroptosis in the pathogenesis of epirubicin-induced cardiotoxicity (EIC) remain elusive. In this study, we aimed to investigate mechanisms underlying ferroptosis-driven EIC. Epirubicin triggered ferroptosis both <em>in vivo</em> and in cultured cardiomyocytes, and pretreatment with ferroptosis inhibitor, Ferrostatin-1(Fer-1) alleviates EIC. Microarray analysis was performed to screen for potential molecules involved in EIC in neonatal primary mouse ventricular cardiomyocytes (NMVMs). We found that the transcript level of ATP6V0A2, a subunit of vacuolar ATPase (V-ATPase), was significantly downregulated when NMVMs were subjected to EPI, which was verified <em>in vivo </em>and <em>in vitro</em> as measured by real-time quantitative reverse transcription PCR (qRT-PCR) and immunoblotting. Intriguingly, overexpression of ATP6V0A2 effectively decreased excessive oxidative stress and lipid-peroxidation accumulation, thereby inhibiting ferroptosis and protecting cardiomyocytes against EIC, as evidenced by functional, enzymatic, and morphological changes. Mechanistically, forced expression of ATP6V0A2 restored lysosomal acidification in EPI-treated cardiomyocytes and protected cardiomyocytes and mice hearts from ferroptosis-driven EIC. In this study, our data elucidate that ferroptosis is involved in EIC, which is ignited by ATP6V0A2-dependent lysosomal acidification dysfunction. Our study provides a new potential therapeutic target for ameliorating EIC.</p>
Data manuscript: Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension
<p>Supporting Information files of manuscript: Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension </p> <p> </p> <p>The values behind the means, standard deviations and values used to build graphs; </p>
Messenger RNA (mRNA)-Based, Personalized Cancer Vaccine Against Neoantigens Expressed by the Autologous Cancer
ClinicalTrials.gov study NCT03480152. IPD Sharing: NO. Countries: 1. Publications: 4.
Data for: Genetic architecture modulates diet induced hepatic mRNA and miRNA expression profiles
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The mRNA expression changes in neonatal primary mouse ventricular cardiomyocytes treated with epirubicin (2uM)
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Data from: LAR1 activates NF-κB signaling pathway by binding to APOC1 mRNA and enhancing its expression: A novel mechanism underlying breast carcinogenesis
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Towards genetic modification of plant-parasitic nematodes: Delivery of macromolecules to male germlines and expression of exogenous mRNA in second stage juveniles
<p>Plant-parasitic nematodes are a current and future threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics. Forward genetics is largely restricted to studies of natural variation in populations, and reverse genetics is entirely reliant on RNA interference. There is an expectation that the development of functional genetic tools would accelerate progress in plant-parasitic nematology, and hence the development of novel control solutions. Here, we develop some of the foundational biology required to deliver a functional genetic "tool kit" in plant-parasitic nematodes. We characterise the gonads of male <em>Heterodera schachtii</em> and Meloidogyne hapla in the context of spermatogenesis. We test and optimise various methods for the delivery, expression, and/or detection of exogenous nucleic acids in plant-parasitic nematodes. We demonstrate that delivery of macromolecules to cyst and root knot nematode male germlines is difficult but possible. Similarly, we demonstrate the delivery of oligonucleotides to root knot nematode gametes. Finally, we develop a transient expression system in plant-parasitic nematodes by demonstrating the delivery and expression of exogenous mRNA encoding various reporter genes throughout the body of <em>H. schachtii</em> juveniles using lipofectamine-based transfection. We anticipate these developments to be independently useful, and, taken together, will expedite the development of genetic modification protocols for sedentary endoparasitic nematodes, and ultimately catalyze research on a group of nematodes that threaten global food security.</p>
Nanostring mRNA expression profiling of P7 Arx(GCG)10+7 neonatal cortex using nCounter® mouse Neuropathology Plus Panel
<p>X-linked infantile spasms syndrome (ISSX) is a clinically devastating developmental epileptic encephalopathy with life-long impact. <em>Arx<sup>(GCG)10+7</sup></em>, a mouse model of the most common triplet-repeat expansion mutation of ARX, exhibits neonatal spasms, electrographic phenotypes and abnormal migration of GABAergic interneuron subtypes. Neonatal presymptomatic treatment with 17β-estradiol (E2) in <em>Arx<sup>(GCG)10+7</sup></em> reduces spasms and modifies progression of epilepsy. Cortical pathology during this period, a crucial point for clinical intervention in ISSX, has largely been unexplored, and the pathogenic cellular defects that are targeted by early interventions are unknown. In the first postnatal week, we identified a transient wave of elevated apoptosis in <em>Arx<sup>(GCG)10+7</sup></em> mouse cortex that is non-Arx cell autonomous, since mutant Arx-immunoreactive (Arx+) cells are not preferentially impacted by cell death. NeuN+ (also known as Rbfox3) survival was also not impacted, suggesting a vulnerable subpopulation in the immature <em>Arx<sup>(GCG)10+7</sup></em> cortex. Inflammatory processes during this period might explain this transient elevation in apoptosis; however, transcriptomic and immunohistochemical profiling of several markers of inflammation revealed no innate immune activation in <em>Arx<sup>(GCG)10+7</sup></em> cortex. Neither neonatal E2 hormone therapy, nor ACTH(1-24), the frontline clinical therapy for ISSX, diminished the augmented apoptosis in <em>Arx<sup>(GCG)10+7</sup></em>, but both rescued neocortical Arx+ cell density. Since early E2 treatment effectively prevents seizures in this model, enhanced apoptosis does not solely account for the seizure phenotype, but may contribute to other aberrant brain function in ISSX. However, since both hormone therapies, E2 and ACTH(1-24), elevate the density of cortical Arx+-interneurons, their early therapeutic role in other neurological disorders hallmarked by interneuronopathy should be explored.</p>
The comparison of marine collagen peptides and soy peptide supplementation on skeletal muscular Calpain-1/2 mRNA expressions and micro-injury in rats
<p>Dataset of a research article named 'The comparison of marine collagen peptides and soy peptide supplementation on skeletal muscular Calpain-1/2 mRNA expressions and micro-injury in rats'.</p>
Dataset: Induction of Bacterial Expression at the mRNA Level by Light
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Slow heating rates increase thermal tolerance and alter mRNA HSP expression in juvenile white sturgeon (Acipenser transmontanus)
<p><span>Fresh</span><span>water fish such as white sturgeon (<em>Acipenser transmontanus</em>) are particularly vulnerable to the effects of anthropogenically induced global warming. Critical thermal maximum tests (CT<sub>max</sub>) are often conducted to provide insight of the impacts of changing temperatures; however, little is known about how the rate of temperature increase in these assays affects thermal tolerance. To assess the effect of heating rate (0.3<span>°C/min, 0.03°C/min, 0.003°C/min) we measured thermal tolerance, somatic indices, and Hsp mRNA expression. </span>Contrary to what has been observed in most other fish species,<span> white sturgeon thermal tolerance</span> was highest at the slowest heating rate of 0.003°C/min, suggesting an ability to rapidly acclimate to slowly increasing temperatures. Hepatosomatic index decreased in all heating rates relative to control fish, indicative of the metabolic costs of thermal stress. <span>At the transcriptional level</span>, slower heating rates resulted in higher mRNA expression of<em> Hsp90a,</em> <em>Hsp90b, </em>and <em>Hsp70. Hsp70 </em>mRNA expression was increased in all heating rates relative to controls, whereas<em> </em>expression of<em> Hsp90a </em>and <em>Hsp90b</em> mRNA only increased in the two slower trials. Together these data indicate that white sturgeon have a very plastic thermal response, which is likely energetically costly to induce. <span>Acute temperature changes may be more detrimental to sturgeon as they struggle to acclimate to rapid changes in their environment, however under slower warming rates they demonstrate strong thermal plasticity to warming.</span></span></p>
Genotype-Driven Treatment of Advanced Non-small Cell Lung Cancer Based on mRNA Expression of ERCC1 & RRM1 as First-line Chemotherapy
ClinicalTrials.gov study NCT01648517. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Efficacy of Tislelizumab and Spartalizumab Across Multiple Cancer-types in Patients with PD1-high MRNA Expressing Tumors
ClinicalTrials.gov study NCT04802876. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.