Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
269
datasets available to search
ShareScore release 0.9.0
Dataset results
269 results for “machinery”
Adipose tissue plasticity in pheochromocytoma patients reveals a key role of the splicing machinery in human adipose browning
<p>RNA-sequencing counts data from omental adipose tissue from control individuals (C1-3) and patients with pheochromocytoma (P1-4) for whole genes (genes-counts.tsv) and individual isoforms (isoform-counts.tsv). Additional details regarding recruited individuals are available in the associated manuscript.</p> <p>Tissue fragments (~150 mg) of adipose biopsies from controls and pheochromocytoma patients were homogenized using a metal bead-based mechanical procedure in a TissueLyser® (QIAGEN, Düsseldorf, Germany). Total RNA was isolated from tissue homogenates using a NucleoSpin® RNA kit (Macherey-Nagel, Dueren, Germany) following the manufacturer’s protocol. mRNA was purified from 2 μg of total RNA using oligo-dT beads; it was then fragmented, retrotranscribed with random primers, and subjected to second-strand synthesis to create double-stranded cDNA fragments. Adaptor ligation, purification of 200-base pair cDNA fragments, amplification of the purified fragments, and library preparation were performed as previously reported by our laboratory. Before sequencing, the RNA integrity number (RIN) of each sample was determined using an Agilent Bioanalyzer 2100; samples with RIN ≥ 7.5 were used for RNA-sequencing. The cDNA library quality and quantity were further analyzed as previously described. Libraries yielding satisfactory results were sequenced on an Illumina HiSeq 2000 sequencer (DNAvision, Charleroi, Belgium). The average reads per sample was 45 million; this level of coverage was previously shown to provide sufficient sequencing depth for gene expression quantification and transcript detection. Quality control of reads was performed using FastQC (version 0.11.8; bioinformatics.babraham.ac.uk/projects/fastqc). Gene expression was quantified using Salmon version 1.1.0 with the additional parameters “– seqBias – gcBias – validateMappings”. GENCODE version 31 (GRCh38.p12) was used as the reference genome and indexed using default parameters; this resulted in 175,775 transcripts corresponding to 35,183 genes.</p>
Videos of farm machinery from above, nadir drone videos.
<p>Used to train AI to detect machinery fleet and other moving objects around robotized tractor. DJI Phantom 4 RTK, about 70 meters altitude. Sample videos.</p>
Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning
<p><em>O-</em>GlcNAcylation is a dynamic post-translational modification that diversifies the proteome. Its dysregulation is associated with neurological disorders that impair cognitive function, and yet identification of phenotype-relevant candidate substrates in a brain-region-specific manner remains unfeasible. By combining an <em>O-</em>GlcNAc binding activity derived from<em> Clostridium perfringens</em> OGA (<em>Cp</em>OGA) with TurboID proximity labeling in <em>Drosophila</em>, we developed an <em>O-</em>GlcNAcylation profiling tool that translates <em>O-</em>GlcNAc modification into biotin conjugation for tissue-specific candidate substrates enrichment. We mapped the <em>O-</em>GlcNAc interactome in major brain regions of <em>Drosophila</em> and found that components of the translational machinery, particularly ribosomal subunits, were abundantly <em>O-</em>GlcNAcylated in the mushroom body of <em>Drosophila</em> brain. Hypo-<em>O-</em>GlcNAcylation induced by ectopic expression of active <em>Cp</em>OGA in the mushroom body decreased local translational activity, leading to olfactory learning deficits that could be rescued by dMyc overexpression-induced increase of protein synthesis. Our study provides a useful tool for future dissection of tissue-specific functions of <em>O-</em>GlcNAcylation in <em>Drosophila</em> and suggests a possibility that <em>O-</em>GlcNAcylation impacts cognitive function via regulating regional translational activity in the brain.</p>
The lysosome origin of biosilica machinery in the Demospongiae model Petrosia ficiformis (Poiret, 1789).
<p>Proteomic LC-MS data for paper "The lysosome origin of biosilica machinery in the Demospongiae model Petrosia ficiformis (Poiret, 1789)."</p>
Cytoplasmic components of the machinery mediating telomere-led rapid chromosome movements in mouse meiosis
<p>Telomere-led rapid chromosome movements (RPMs) are a prominent characteristic of chromosome dynamics during meiosis. Although of crucial importance in maintaining germ cell integrity, the extranuclear portion of the machinery supporting RPMs in mammals is poorly understood. Using an unbiased proteomic approach to identify motor proteins associated to microtubules in mouse meiotic cells, complemented with co-immunoprecipitation confirmation, we uncovered kinesins as candidate of the machinery mediating RPMs in mouse spermatocytes. Further biochemical, microscopy, and functional analysis shows that KIF5B and KIF2B interact with KASH5 and act as motor proteins mediating the LINC complex-microtubule interactions. Our results show that member of the kinesin family of molecular motors act as novel critical modules of the machinery promoting complex dynamic chromosomes in mammals.</p>
Dataset: Titan Machinery Inc. (TITN) Stock Performance
This dataset provides historical stock market performance data for specific companies. It enables users to analyze and understand the past trends and fluctuations in stock prices over time. This information can be utilized for various purposes such as investment analysis, financial research, and market trend forecasting.
Exploring Host-Binding Machineries of Mycobacteriophages with AlphaFold2
<p>Coordinates of AlphaFold2 predicted structures presented in the publication "Exploring Host-Binding Machineries of Mycobacteriophages with AlphaFold2".</p>
Membrane interaction and mechanism of LC3 lipidation machinery in autophagy raw GUV data
<p>Raw GUV data of fluorescent protein imaged on a Nikon A1 confocal microscope with a 63 × Plan 359 Apochromat 1.4 NA objective. Three biological replicates were performed for each experimental 360 condition. Identical laser power and gain settings were used during the course of all conditions.</p>
Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning
Open the record for dataset details and reuse information.
Niche differentiation and evolution of the wood decay machinery in the invasive fungus Serpula lacrymans
<p>Ecological niche breadth and the mechanisms facilitating its evolution are fundamental to understanding adaptation to changing environments, persistence of generalist and specialist lineages and the formation of new species. Woody substrates are structurally complex resources utilized by organisms with specialized decay machinery. Wood-decaying fungi represent ideal model systems to study evolution of niche breadth, as they vary greatly in their host range and preferred decay stage of the substrate. In order to dissect the genetic basis for niche specialization in the invasive brown rot fungus <i>Serpula lacrymans</i>, we used phenotyping and integrative analysis of phylogenomic and transcriptomic data to compare this species to wild relatives in the Serpulaceae with a range of specialist to generalist decay strategies. Our results indicate specialist species have rewired regulatory networks active during wood decay towards decreased reliance on enzymatic machinery, and therefore nitrogen-intensive decay components. This shift was likely accompanied with adaptation to a narrow tree line habitat and switch to a pioneer decomposer strategy, both requiring rapid colonization of a nitrogen-limited substrate. Among substrate specialists with narrow niches, we also found evidence for pathways facilitating reversal to generalism, highlighting how evolution may move along different axes of niche space.</p>
Stoates Mill Machinery
Machinery next to the old Stoates Mill, Watchet. Couldn't get all the way around it as there was a car parked on the left hand side. Shot with a Canon 700D on the 6th of August, 2022. Rendered in Reality Capture. Source: Objaverse 1.0 / Sketchfab
Large Machinery at 4700' in Mine La Motte
This a model of the large machinery that can be found 4700' from the entrance of Mine La Motte, east of the third tee. Source: Objaverse 1.0 / Sketchfab
Molecular dynamics simulation input files: Histone Tail Electrostatics Modulate E2-E3 Enzyme Dynamics: A Gateway to Regulate Ubiquitination Machinery
<p>Molecular dynamics simulation input files: Histone Tail Electrostatics Modulate E2-E3 Enzyme Dynamics: A Gateway to Regulate Ubiquitination Machinery (<a href="https://zenodo.org/record/7423328">https://zenodo.org/record/7423328</a>)</p>
Alphafold2 modeling of KCTD10 interactions with RNAPII machinery
Open the record for dataset details and reuse information.
Niche differentiation and evolution of the wood decay machinery in the invasive fungus Serpula lacrymans
Open the record for dataset details and reuse information.
Exploring Tools for Sharing and Trading in Agricultural Machinery Rental: Insights from Gray Literature Review
<p>This spreadsheet contains data from the analysis of platforms for renting and selling agricultural machinery identified during the research. It includes overall evaluations as well as individual assessments from three evaluators, based on predefined criteria for qualitative analysis. The recorded information includes scores assigned to each evaluated site, reflecting the implementation or lack thereof of specific features, such as filter-based search, review systems, and other functionalities related to the sharing economy model in the agricultural sector. The data is organized to facilitate comparison among evaluators and overall conclusions.</p>
Osiris: a platform for advertising agricultural machinery rentals
<p>This dataset contains three spreadsheets of heuristic evaluations conducted by experts and also the prototypes they used. The evaluations assess the usability and effectiveness of various systems based on established heuristic principles. Each spreadsheet includes detailed assessments, observations, and ratings provided by the experts, offering valuable insights into the user interface and experience. These evaluations are intended to support research and improvement of usability practices in the context of the evaluated systems.</p>
Evaluating the potential of Digital Twin technology on pharmaceutical manufacturing efficiency in Ireland: An in-depth analysis of Machinery Validation
<p>The dissertation titled <strong>"Evaluating the Potential of Digital Twin Technology on Pharmaceutical Manufacturing Efficiency in Ireland: An In-depth Analysis of Machinery Validation"</strong> explores the transformative impact of Digital Twin (DT) technology within Ireland's pharmaceutical manufacturing sector. Conducted by MSc candidate Gayathri Gopakumar at Griffith College Dublin, the research focuses on how DT technology can enhance machinery validation processes, thereby improving operational efficiency and ensuring regulatory compliance.</p> <p>Employing a qualitative research methodology, the study includes semi-structured interviews with industry experts to gather insights into the current awareness, perceived benefits, and challenges associated with DT adoption in the pharmaceutical industry. The research aims to provide a comprehensive understanding of DT technology's role in machinery validation and its broader implications for manufacturing efficiency.</p> <p>This work contributes to the existing body of knowledge by offering a detailed analysis of DT technology's potential applications in pharmaceutical manufacturing, with a specific focus on the Irish context. It serves as a valuable resource for professionals and researchers interested in the integration of advanced digital technologies in the pharmaceutical sector.</p>
Exploring the structural dynamics of the vesicle priming machinery
<p>Here are the pdb files for the simulations with only 6 central SNAREs and 6 central and 6 peripheral SNAREs with C2B of Syt1 and residue 51-75 of Cpx1 shown in figure 3.</p> <p>File for figure 3a: ic_6c_snares.pdb is the initial condition for simulations with only 6 central SNAREs, and the tcl file figure_3a.tcl is to show this initial condition.</p> <p>File for figure 3b: ic_6c6p_snares_c2b.pdb is the initial condition for simulations with 6 central and 6 peripheral SNAREs with a vesicle, and the tcl file figure_3b_top_view.tcl and figure_3b_side_view.tcl are to show this initial condition.</p> <p>File for figure 3c: ic_6c6p_snares_c2b_nd.pdb is the initial condition for simulations with 6 central and 6 peripheral SNAREs with a nanodisc and ic_6c6p_snares_c2b_nd.xtc file is the corresponding trajectory of this simulation. The tcl file movie_for_figure_3c.tcl is to show the movie of this simulation. If you want to look at all proteins in this simulation, the tcl file movie_for_figure_3c_all.tcl can do that.</p> <p>To view these snapshots by VMD, load the corresponding .tcl file or run "vmd -e name.tcl" in your terminal.</p>
Evolution of ubiquitin, cytoskeleton, and vesicular trafficking machinery in giant viruses
<p>This repository contains genomes, proteins, and alignments used in the study "Evolution of ubiquitin, cytoskeleton, and vesicular trafficking machinery in giant viruses".</p> <p> </p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.