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311 results for “macrophage polarization”
Dynamics of macrophage polarization in Salmonella infection : Raw data
<p>Experimental raw data of the paper "Dynamics of macrophage polarization support <em>Salmonella</em> persistence in a whole living organism", Leiba et al.</p>
Dynamics of macrophage polarization in Salmonella infection: row data
<p>Experimental metadata of the manuscript " <strong>Dynamics of macrophage polarization support <em>Salmonella</em> persistence in a whole living organism</strong> " by Leiba et al.</p>
SYSTEMS LEVEL IDENTIFICATION OF A MATRISOME-ASSOCIATED MACROPHAGE POLARIZATION STATE IN MULTI-ORGAN FIBROSIS
<p>This Zenodo repository contains the processed Seurat objects for "SYSTEMS LEVEL IDENTIFICATION OF A MATRISOME-ASSOCIATED MACROPHAGE POLARIZATION STATE IN MULTI-ORGAN FIBROSIS".</p> <p>The Seurat objects include:</p> <ul> <li>Tissue-specific monocytes / macrophages (used in Figure 1,3,4) <ul> <li>endo.rds: Endometrium monocytes / macrophages</li> <li>heart.rds: Heart monocytes / macrophages</li> <li>kidney.rds: Kidney monocytes / macrophages</li> <li>liver.rds: Liver monocytes / macrophages</li> <li>lung.rds: Lung monocytes / macrophages</li> <li>skin.rds: Skin monocytes / macrophages</li> </ul> </li> <li>SPP1 macrophages across all six tissues (used in Figure 2,3,4,5) <ul> <li>SPP1seu.rds</li> </ul> </li> </ul> <p>Code for generating these objects can be found at: https://github.com/the-ouyang-lab/mam-reproducibility</p>
Data from: High-throughput mRNA sequencing-based DEGs: HNF4A mitigates sepsis-associated lung injury by upregulating NCOR2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype
Open the record for dataset details and reuse information.
Dataset related to article "Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism."
<p>BACKGROUND:</p> <p>Thyroid carcinoma includes several variants characterized by different biological and clinical features: from indolent microcarcinoma to undifferentiated and aggressive anaplastic carcinoma. Inflammation plays a critical role in thyroid tumors. Conditions predisposing to cancer, as well as oncogene activity, contribute to the construction of an inflammatory microenvironment that facilitates thyroid tumor progression. Moreover, oncogene-induced senescence, a mechanism tightly connected with inflammation, and able to restrain or promote cancer progression, is involved in thyroid cancer. The interactions between thyroid tumor cells and the microenvironment are not completely clarified.</p> <p>METHODS:</p> <p>We characterize in vitro the interplay between macrophages and senescent thyrocytes and tumor-derived cell lines, modeling early and late thyroid tumor stages, respectively. Purified peripheral blood-derived human monocytes were exposed to thyroid cell-derived conditioned medium (CM) and assessed for phenotype by flow cytometry. The factors secreted by thyroid cells and macrophages were identified by gene expression analysis and ELISA. The protumoral effect of macrophages was assessed by wound healing assay on K1 thyroid tumor cells. The expression of PTGS2 and M2 markers in thyroid tumors was investigated in publicly available datasets.</p> <p>RESULTS:</p> <p>Human monocytes exposed to CM from senescent thyrocytes and thyroid tumor cell lines undergo M2-like polarization, showing high CD206 and low MHC II markers, and upregulation of CCL17 secretion. The obtained M2-like macrophages displayed tumor-promoting activity. Among genes overexpressed in polarizing cells, we identified the prostaglandin-endoperoxide synthase enzyme (PTGS2/COX-2), which is involved in the production of prostaglandin E2 (PGE2). By using COX-2 inhibitors we demonstrated that the M2-like polarization ability of thyroid cells is related to the production of PGE2. Co-expression of PTGS2 and M2 markers is observed a significant fraction of human thyroid tumors.</p> <p>CONCLUSIONS:</p> <p>Our results demonstrate that both senescent thyrocytes and thyroid tumor cell lines trigger M2-like macrophage polarization that is related to PGE2 secretion. This suggests that the interaction with the microenvironment occurs at both early and late thyroid tumor stages, and favors tumor progression. The co-expression of PTGS2 gene and M2 markers in human thyroid carcinoma highlights the possibility to counteract tumor growth through COX-2 inhibition.</p>
Raw data to "Macrophage Plasticity and Polarization Are Altered in the Experimental Model of Multiple Sclerosis"
<p>Background: Macrophages have been identified as one of the major effectors of inflammation and demyelination in both MS and its animal model, experimental autoimmune encephalomyelitis (EAE). However, the activation and heterogeneity of macrophages in MS is not fully understood.</p> <p>Results: We performed a complete immunophenotyping of M1 and M2 macrophages from EAE and control mice through polychromatic flow cytometry. We found that M1 macrophages possessed a higher proinflammatory profile in EAE compared to control mice, since they expressed higher levels of activation/co-stimulatory markers (iNOS, CD40 and CD80) (Table 1), cytokines/chemokines (IL-6, IL-12, CCL2 and CXCL10) (Table 2) and a higher expression of Toll-like receptors (TLR2) 2 and 5 (Table 3). On the contrary, M2 of EAE mice lost their M2-like phenotype by showing a decreased expression of their signature markers CD206, CD11c, CD44 and CCL22, and a concomitant upregulation of several M1 makers such as iNOS, CD40, CD80/CD86 (Table 1) and proinflammatory CCL22 (Table 2) and TLR4 and 8.</p> <p>Conclusions: Our data account for a phenotypic alteration of M1/M2 balance during MS and this can be of crucial importance not only for a better understanding of the immunopathology of this neurodegenerative disease but also to potentially develop new macrophage-centered therapeutic strategies</p>
Fig. 4. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 4. S. frutescens inhibits LPS-induced HMOX-1 upregulation in RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in duplicate, +++ = p <0.001 compared to DMSO control; *** = p <0.001 compared to LPS control; ## = p <0.01 ethanolic extract compared to hot aqueous extract.
Fig. 5 in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 5. Effect of S. frutescens on A) CD86 (M1) and B) CD206 (M2) expression in LPS-induced RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in triplicate++ = p <0.05, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.
Fig. 3. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 3. S. frutescens modulated the expression of pro-inflammatory cytokines in LPS-induced Raw 264.7 cells. Data represent mean ± SEM of three independent experiments, ++ = p <0.05, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared with LPS control; ### = p <0.001 ethanolic extract compared to hot aqueous extract.
Fig. 1. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 1. S.frutescens reduces (A) NO and (B) ROS production in LPS-induced RAW 264.7 cells. 10 μM Curcumin was used as an anti-inflammatory control. Data represent mean ± SEM of three independent experiments,each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05,** = p <0.01,*** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.
Fig. 2. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 2. S. frutescens reduces (A) iNOS and (B) COX-2 expression in LPS-induced RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.
Fig. 6. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways
Fig. 6. S. frutescens inhibits NFKB and MAPK activation in LPS-induced RAW 264.7 cells. (A) Quantitative analysis of nuclear NFKB localization determined using the cell scoring analysis module on MetaXpress® software (Version 5.1) of the ImageXpress Micro XLS (Molecular Devices, USA), with expression recorded as percentage of LPS induction. Changes in phosphorylation level of (B) p-p65 NFKB, (C) p-ERK 1/2 and (D) p-p38 detected by flow cytometry analysis, with expression recoded as percentage of untreated control. Data represents mean ± SEM of three independent experiments, each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract. Corresponding immunofluorescence images of control cells (Ei), LPS induced cells (Eii), LPS induced cells co-treated with 50 μg mL −1 and 200 μg mL −1 hot aqueous S. frutescens extract (Eiii, Eiv), LPS induced cells co-treated with 50 μg mL −1 and 200 μg mL −1 ethanolic S. frutescens extract (Ev, Evi). Cells were stained with anti-p65 NFKB antibody and co-stained with DAPI. Increased nuclear localization and increased cytoplasmic expression is seen following LPS treatment (Eii), which is reduced following co-treatment with S. frutescens (Eiii-Evi). Scale bar = 50 μm.
Cocarnit Effects on Macrophages Polarization
ClinicalTrials.gov study NCT03877523. IPD Sharing: NO. Countries: 1. Publications: 4.
Macrophage Polarization in Response to Macronutrient Intake in Healthy Humans: A Randomized Clinical Study
ClinicalTrials.gov study NCT03173677. IPD Sharing: NO. Countries: 0. Publications: 12.
Integrative Proteome, Transcriptome and DNA Methylome Analysis of Regulatory Networks During U937-derived Macrophage Polarization from an M2 to M1 Phenotype [array]
GEO Series GSE127981. Homo sapiens. 6 samples. Type: Expression profiling by array; Non-coding RNA profiling by array.
Investigating the effects of different HMGB1 redox isoforms on macrophage polarization
GEO Series GSE200210. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
Diabetes exacerbated sepsis-induced intestinal injury by promoting M1 Macrophage Polarization via miR-3061/Snail1 signaling.
GEO Series GSE202261. Mus musculus. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Targeting BRD4 Ameliorates Experimental Emphysema Via Suppressing both M1 and M2 alveolar macrophage polarization-1[RNA-seq]
GEO Series GSE248962. Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.
Methylation and Transcriptomic Profiling Reveals Short Term and Long Term Regulatory Responses in Polarized Macrophages [RNA-Seq]
GEO Series GSE273627. Homo sapiens. 27 samples. Type: Expression profiling by high throughput sequencing.
Identification of pharmacologic inhibitors and activators of IL-4-induced macrophage polarization [THP-1 RNA-seq]
GEO Series GSE154346. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.