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45 results for “microbial infection”
Identification of microbial taxa present in Ctenocephalides felis (cat flea) reveals widespread co-infection and associations with vector phylogeny
<p><strong>Background </strong></p> <p><em>Ctenocephalides</em> <em>felis</em>, the cat flea, is the most common ectoparasite of cats and dogs worldwide. As a cause of flea allergy dermatitis and a vector for two genera of zoonotic pathogens (<em>Bartonella</em> and <em>Rickettsia</em> spp.), the effect of the <em>C</em>. felis <em>microbiome</em> on pathogen transmission and vector survival is of substantial medical importance to both human and veterinary medicine. The aim of this study was to assay the pathogenic and commensal eubacterial microbial communities of individual <em>C</em>. <em>felis</em> from multiple geographic locations and analyze these findings by location, qPCR pathogen prevalence, and flea genetic diversity.</p> <p><strong>Methods </strong></p> <p>16S Next Generation Sequencing (NGS) was utilized to sequence the microbiome of fleas collected from free-roaming cats, and the <em>cox1</em> gene was used for flea phylogenetic analysis. NGS data were analyzed for 168 individual fleas from seven locations within the US and UK. Given inconsistency in the genera historically reported to constitute the <em>C</em>. <em>felis</em> microbiome, we utilized the decontam prevalence method followed by literature review to separate contaminants from true microbiome members.</p> <p><strong> Results </strong></p> <p>NGS identified a single dominant and cosmopolitan amplicon sequence variant (ASV) from <em>Rickettsia</em> and <em>Wolbachia</em> while identifying one dominant <em>Bartonella</em> <em>clarridgeiae</em> and one dominant <em>Bartonella henselae/Bartonella</em> <em>koehlerae</em> ASV. Multiple less common ASVs from these genera were detected within restricted geographical ranges. Co-detection of two or more genera (<em>Bartonella</em>, <em>Rickettsia</em>, and/or <em>Wolbachia</em>) or multiple ASVs from a single genus in a single flea was common. <em>Achromobacter</em>, <em>Peptoniphilus</em>, and <em>Rhodococcus</em> were identified as additional candidate members of the <em>C</em>. <em>felis</em> microbiome on the basis of decontam analysis and literature review. <em>Ctenocephalides</em> <em>felis</em> phylogenetic diversity as assessed by the <em>cox1</em> gene fell within currently characterized clades while identifying seven novel haplotypes. NGS sensitivity and specificity for <em>Bartonella</em> and <em>Rickettsia</em> spp. DNA detection was compared to targeted qPCR.</p> <p><strong>Conclusions </strong></p> <p>Our findings confirm the widespread coinfection of fleas with multiple bacterial genera and strains, proposing three additional microbiome members. The presence of minor <em>Bartonella</em>, <em>Rickettsia</em>, and <em>Wolbachia</em> ASVs was found to vary by location and flea haplotype. These findings have important implications for flea-borne pathogen transmission and control. </p>
Identification of microbial taxa present in Ctenocephalides felis (cat flea) reveals widespread co-infection and associations with vector phylogeny
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Data from: Fungal endophyte‐infected leaf litter alters in‐stream microbial communities and negatively influences aquatic fungal sporulation
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Data from: Variation in gut microbial communities and its association with pathogen infection in wild bumble bees (Bombus)
Bacterial gut symbiont communities are critical for the health of many insect species. However, little is known about how microbial communities vary among host species or how they respond to anthropogenic disturbances. Bacterial communities that differ in richness or composition may vary in their ability to provide nutrients or defenses. We used deep sequencing to investigate gut microbiota of three species in the genus Bombus (bumble bees). Bombus are among the most economically and ecologically important non-managed pollinators. Some species have experienced dramatic declines, probably due to pathogens and land-use change. We examined variation within and across bee species and between semi-natural and conventional agricultural habitats. We categorized as 'core bacteria' any operational taxonomic units (OTUs) with closest hits to sequences previously found exclusively or primarily in the guts of honey bees and bumble bees (genera Apis and Bombus). Microbial community composition differed among bee species. Richness, defined as number of bacterial OTUs, was highest for B. bimaculatus and B. impatiens. For B. bimaculatus, this was due to high richness of non-core bacteria. We found little effect of habitat on microbial communities. Richness of non-core bacteria was negatively associated with bacterial abundance in individual bees, possibly due to deeper sampling of non-core bacteria in bees with low populations of core bacteria. Infection by the gut parasite Crithidia was negatively associated with abundance of the core bacterium Gilliamella and positively associated with richness of non-core bacteria. Our results indicate that Bombus species have distinctive gut communities, and community-level variation is associated with pathogen infection.
Data from: Heterozygosity at neutral and immune loci is not associated with neonatal mortality due to microbial infection in Antarctic fur seals
Numerous studies have reported correlations between the heterozygosity of genetic markers and fitness. These heterozygosity fitness correlations (HFCs) play a central role in evolutionary and conservation biology, yet their mechanistic basis remains open to debate. For example, fitness associations have been widely reported at both neutral and functional loci, yet few studies have directly compared the two, making it difficult to gauge the relative contributions of genome-wide inbreeding and functional genetic variation to fitness. Here, we compared the effects of neutral and immune gene heterozygosity on death from bacterial infection in Antarctic fur seal (Arctocephalus gazella) pups. We specifically developed a panel of 13 microsatellites from expressed immune genes and genotyped these together with 48 neutral loci in 234 individuals, comprising 39 pups that were classified at necropsy as having most likely died of bacterial infection together with a five times larger matched sample of healthy surviving pups. Identity disequilibrium quantified from the neutral markers was positive and significant, indicative of variance in inbreeding within the study population. However, multilocus heterozygosity did not differ significantly between healthy and infected pups at either class of marker, and little evidence was found for fitness associations at individual loci. These results support a previous study of Antarctic fur seals that found no effects of heterozygosity at nine neutral microsatellites on neonatal survival, and thereby help to refine our understanding of how HFCs vary across the life cycle. Given that non-significant HFCs are underreported in the literature, we also hope that our study will contribute towards a more balanced understanding of the wider importance of this phenomenon.
Rapid Point-of-care Bacteriuria and Microbial Susceptibility for Women With Suspected Uncomplicated Urinary Tract Infections: Diagnostic Accuracy in General Practice.
ClinicalTrials.gov study NCT06625268. IPD Sharing: NO. Countries: 1. Publications: 2.
Diagnosis of Septicaemia by Detection of Microbial DNA in Blood in Severe Infections
ClinicalTrials.gov study NCT00709358. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Effects of Anti-microbial Spray Dressing in Preventing Tenckhoff Catheter Exit Site Infection for End Stage Renal Failure Patients
ClinicalTrials.gov study NCT01952964. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Fecal Microbial Transplantation in Critically Ill Patients With Severe Infections.
ClinicalTrials.gov study NCT05578196. IPD Sharing: NO. Countries: 1. Publications: 1.
Data from: Variation in gut microbial communities and its association with pathogen infection in wild bumble bees (Bombus)
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Data from: Opposite effects of anthelmintic treatment on microbial infection at individual versus population scales
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Data from: Macroparasites at peripheral sites of infection are major and dynamic modifiers of systemic anti-microbial pattern recognition responses
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Data from: Heterozygosity at neutral and immune loci is not associated with neonatal mortality due to microbial infection in Antarctic fur seals
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Pattern of Microbial Infection in AECOPD Patients and Its Sensitivity to Antibiotics
ClinicalTrials.gov study NCT06123780. IPD Sharing: Not stated. Countries: 0. Publications: 5.
Epidemiology of Resistant Microbial Strains Among Different Groups of People (Healthy, Infected and Exposed to Animals)
ClinicalTrials.gov study NCT03343119. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Effect of microbial β-glucuronidases on Citrobacter rodentium mice infection
GEO Series GSE252823. Mus musculus. 20 samples. Type: Expression profiling by high throughput sequencing.
Microbial infection instigates tau-related pathology in Alzheimer's disease via activating neuroimmune cGAS-STING pathway
GEO Series GSE264504. Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
Tissue damage signaling is essential for protective neutrophil recruitment to microbial infection in zebrafish (Danio rerio)
GEO Series GSE111528. Danio rerio. 9 samples. Type: Expression profiling by high throughput sequencing.
Protection against maternal infection-associated fetal growth restriction: proof-of-concept with a microbial-derived immunomodulator
GEO Series GSE85414. Mus musculus. 48 samples. Type: Expression profiling by high throughput sequencing.
Glucose competition between immune cells and microbial pathogens controls infections
GEO Series GSE99904. Candida albicans; Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.