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34 results for “mitochondrial DNA genomes”
Datasets: Population genomics and mitochondrial DNA reveal cryptic diversity in North American Spring Cavefishes (Amblyopsidae, Forbesichthys)
<p>Forbesichthys_allsites.vcf: Dataset in VCF format used to perform Effective Population Size estimation.</p> <p>Forbesichthys_SNPs_NoLD.vcf: Dataset in VCF format used to perform PCA, fastStrucuture, and phylogenetic analyses.</p> <p>Files with extension .sfs contain site spectrum frequencies generated with the program easySFS.py.</p>
Genome-scale target capture of mitochondrial and nuclear environmental DNA from water samples
<p>Environmental DNA (eDNA) provides a promising supplement to traditional sampling methods for population genetic inferences, but current studies have almost entirely focused on short mitochondrial markers. Here, we develop one mitochondrial and one nuclear set of target capture probes for the whale shark (<i>Rhincodon typus</i>) and test them on seawater samples collected in Qatar to investigate the potential of target capture for eDNA-based population studies. The mitochondrial target capture successfully retrieved ~235x (90x-352x per base position) coverage of the whale shark mitogenome. Using a minor allele frequency of 5%, we find 29 variable sites throughout the mitogenome, indicative of at least five contributing individuals. We also retrieved numerous mitochondrial reads from an abundant non-target species mackerel tuna<i> </i>(<i>Euthynnus affinis</i>), showing a clear relation between sequence similarity to the capture probes and the number of captured reads. The nuclear target capture probes retrieved only few reads and polymorphic variants from the whale shark, but we successfully obtained millions of reads and thousands of polymorphic variants with different allele frequencies from <i>E</i>. <i>affinis</i>. We demonstrate that target capture of complete mitochondrial genomes and thousands of nuclear loci is possible from aquatic eDNA samples. Our results highlight that careful probe design, taking into account the range of divergence between target and non-target sequences as well as presence of non-target species at the sampling site, is crucial to consider. Environmental DNA sampling coupled with target capture approaches provide an efficient means with which to retrieve population genomic data from aggregating and spawning aquatic species.</p>
Data from: Strong selective effects of mitochondrial DNA on the nuclear genome
<p>Oxidative phosphorylation, the primary source of cellular energy in eukaryotes, requires gene products encoded in both the nuclear and mitochondrial genomes. As a result, functional integration between the genomes is essential for efficient adenosine triphosphate (ATP) generation. Although within populations this integration is presumably maintained by coevolution, the importance of mitonuclear coevolution in key biological processes such as speciation and mitochondrial disease has been questioned. In this study, we crossed populations of the intertidal copepod <i>Tigriopus californicus</i> to disrupt putatively coevolved mitonuclear genotypes in reciprocal F<sub>2</sub> hybrids. We utilized inter-individual variation in developmental rate among these hybrids as a proxy for fitness to assess the strength of selection imposed on the nuclear genome by alternate mitochondrial genotypes. Developmental rate varied among hybrid individuals, and <i>in vitro </i>ATP synthesis rates of mitochondria isolated from high fitness hybrids were approximately two-fold greater than those of mitochondria isolated from low fitness individuals. We then used Pool-seq to compare nuclear allele frequencies for high or low fitness hybrids. Significant biases for maternal alleles were detected on five (of 12) chromosomes in high fitness individuals of both reciprocal crosses, whereas maternal biases were largely absent in low fitness individuals. Therefore, the most fit hybrids were those with nuclear alleles that matched their mitochondrial genotype on these chromosomes, suggesting that mitonuclear effects underlie individual-level variation in developmental rate and that inter-genomic compatibility is critical for high fitness. We conclude that mitonuclear interactions can have profound impacts on both physiological performance and the evolutionary trajectory of the nuclear genome.</p>
Data from: Hare pseudo-reference genome from: the genomic impact of historical hybridization with massive mitochondrial DNA introgression
<p><b>Background:</b> The extent to which selection determines interspecific patterns of genetic exchanges enlightens the role of adaptation in evolution and speciation. Often reported extensive interspecific introgression could be selection-driven, but also result from demographic processes, especially in cases of invasive species replacements, which can promote introgression at their front. Because invasion and selective sweeps similarly mold variation, population genetics evidence for selection can only be gathered in an explicit demographic framework. The Iberian hare, <i>Lepus granatensis</i>, displays in its northern range extensive mitochondrial DNA introgression from <i>L. timidus</i>, an arctic/boreal species that it replaced locally after the last glacial maximum. We use whole-genome sequencing to infer geographic and genomic patterns of nuclear introgression and fit a neutral model of species replacement with hybridization, allowing us to evaluate how selection influenced introgression genome-wide, including for mtDNA.</p> <p><b>Results:</b> Although the average nuclear and mtDNA introgression patterns are strongly contrasted, they fit a single neutral model of post-glacial invasive replacement of <i>timidus</i> by <i>granatensis</i>. Outliers of elevated introgression include several genes related to immunity, spermatogenesis, and mitochondrial metabolism. Introgression is reduced on the X-chromosome and in low recombining regions.</p> <p><b>Conclusion:</b> General nuclear and mtDNA patterns of introgression can be explained by purely demographic processes. Hybrid incompatibilities and interplay between selection and recombination locally modulate levels of nuclear introgression. Selection promoted introgression of some genes involved in conflicts, either interspecific (parasites) or possibly cytonuclear. In the latter case, nuclear introgression could mitigate the potential negative effects of alien mtDNA on mitochondrial metabolism and male-specific traits.</p>
Data and scripts for the manuscript of svaRetro and svaNUMT: modular packages for annotating retrotransposed transcripts and nuclear integration of mitochondrial DNA in genome sequencing data
<p>This upload include data and scripts supporting the results described in the manuscript of <em>svaRetro and svaNUMT: modular packages for annotating retrotransposed transcripts and nuclear integration of mitochondrial DNA in genome sequencing data</em><em>. </em>Detailed description of the contents can be found in README.txt.</p>
Data from: Genomics overrules mitochondrial DNA, siding with morphology on a controversial case of species delimitation
Species delimitation is a major quest in biology and is essential for adequate management of the organismal diversity. A challenging example comprises the fish species of red snappers in the Western Atlantic. Red snappers have been traditionally recognized as two separate species based on morphology: Lutjanus campechanus (northern red snapper) and L. purpureus (southern red snappers). Recent genetic studies using mitochondrial markers, however, failed to delineate these nominal species, leading to the current lumping of the northern and southern populations into a single species (L. campechanus). This decision carries broad implications for conservation and management as red snappers have been commercially over-exploited across the Western Atlantic and are currently listed as vulnerable. To address this conflict, we examine genome-wide data collected throughout the range of the two species. Population genomics, phylogenetic and coalescent analyses favor the existence of two independent evolutionary lineages, a result that confirms the morphology-based delimitation scenario in agreement with conventional taxonomy. While we find evidence of introgression in geographically neighboring populations in northern South America, the genetic differences strongly support isolation and differentiation of these species, suggesting that the northern and southern red snappers should be treated as distinct taxonomic entities.
Genome-scale target capture of mitochondrial and nuclear environmental DNA from water samples
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Data from: Genomics overrules mitochondrial DNA, siding with morphology on a controversial case of species delimitation
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Data from: Strong selective effects of mitochondrial DNA on the nuclear genome
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Data from: The genomic impact of historical hybridization with massive mitochondrial DNA introgression
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Supporting data for: "mtGrasp: Streamlined mitochondrial genome reference-grade assembly and standardization to enhance mitogenome resources and improve the development of environmental DNA assays"
<p>Here, we provide supporting data for the manuscript "mtGrasp: Streamlined mitochondrial genome reference-grade assembly and standardization to enhance mitogenome resources and improve the development of environmental DNA assays".</p> <p>Phylogenetic_analysis.tar.gz contains the script and fasta files used for the phylogenetic analysis, and Mitogenomes.tar.gz contains the mitochondrial sequences utilized that are not publicly available in GenBank.</p>
Figure 2 in Complete mitochondrial genome of Tetraophasis szechenyii Madarász, 1885 (Aves: Galliformes: Phasianidae), and its genetic variation as inferred from the mitochondrial DNA Control Region
Figure 2. Median-joining network of all the control region haplotypes found in Tetraophasis szechenyii. Notes: Missing haplotypes in the network are represented by black dots; circle sizes are proportional to the number of individuals sharing the same haplotypes (n); each mutation step is shown as a short line connecting neighbouring haplotypes; numbers of mutations between haplotypes are indicated near branches if greater than 1.
Figure 1 in Complete mitochondrial genome of Tetraophasis szechenyii Madarász, 1885 (Aves: Galliformes: Phasianidae), and its genetic variation as inferred from the mitochondrial DNA Control Region
Figure 1. Molecular phylogenetic tree derived from the complete DNA sequences of 12 mitochondrial protein-coding genes using Bayesian inference and maximum likelihood analyses. Notes:The numbers beside the nodes are Bayesian posterior probabilities (≥ 0.9 retained) and bootstrap proportions of maximum likelihood analyses calculated with 100 replicates (≥ 50% retained); Anas platyrhynchos and Alectura lathami were set as outgroups; *clades not supported by Bayesian inference.
Data from: Deep sequencing of mixed total DNA without barcodes allows efficient assembly of highly plastic ascidian mitochondrial genomes
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Delimiting cryptic species within the brown-banded bamboo shark, Chiloscyllium punctatum in the Indo-Australian region with mitochondrial DNA and genome-wide SNP approaches
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Data from: Targeted multiplex next-generation sequencing: Advances in techniques of mitochondrial and nuclear DNA sequencing for population genomics
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Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA
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Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes
GEO Series GSE42077. Homo sapiens. 11 samples. Type: Expression profiling by array.
Genome-wide methylation profile of mitochondrial DNA across bovine preimplantation development
GEO Series GSE230476. Bos taurus. 18 samples. Type: Methylation profiling by high throughput sequencing.
Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes.
GEO Series GSE42271. Homo sapiens. 15 samples. Type: Genome variation profiling by SNP array.
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