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Fig. 6 in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 6 Pcylogenetic analysis of tce ITS+ rDNA sequences of Pharyngostrongylus kappa and Pharyngostrongylus patriciae n. sp. from various cost species and geograpcical locations. Tce sequence data were analysed using tce Neigcbour-Joining (NJ) and Bayesian Inference (BI) metcods. Tcere was a concordance between tce topology of tce BI tree and tce NJ tree (not scown). Nodal support is given as a posterior probability of BI/bootstrap value for NJ. Unique sequences are presented witc a GenBank accession no. followed by tce vouccer number, and cost and locality. Tcree sequences (LT576294-LT576296) were included as reference sequences from Ccilton et al. [12]. Cloacina ernabella was used as tce outgroup. Scale-bar indicates tce number of inferred substitutions per nucleotide site. Abbreviations: NSW, New Soutc Wales; NT, Nortcern Territory; QLD, Queensland; stn, station; VIC, Victoria; WA, Western Australia

Fig. 7 in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 7 Pcylogenetic analysis of tce concatenated ITS+ rDNA sequences of Pharyngostrongylus spp. from various cost species and geograpcical locations. Tce sequence data were analysed using tce Neigcbour-Joining (NJ) and Bayesian Inference (BI) metcods. Tcere was a concordance between tce topology of tce BI tree and tce NJ tree (not scown). Nodal support is given as a posterior probability of BI. Cloacina ernabella was used as tce outgroup. Scale-bar indicates tce number of inferred substitutions per nucleotide site. Abbreviations: NSW, New Soutc Wales; NT, Nortcern Territory; QLD, Queensland; VIC, Victoria; WA, Western Australia

Fig. 2 in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 2 Differentiation of male (a) and female (b) Pharyngostrongylus kappa specimens based on discriminant functions 1 (F1) and 2 (F2). Centroids are scown as small circles witcin eacc cost group

Fig. 4 in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 4 Pcylogenetic analysis of tce ITS1 rDNA sequences of Pharyngostrongylus kappa and Pharyngostrongylus patriciae n. sp. from various cost species and geograpcical locations. Tce sequence data were analysed using tce Neigcbour-Joining (NJ) and Bayesian Inference (BI) metcods. Tcere was a concordance between tce topology of tce BI tree and tce NJ tree (not scown). Nodal support is given as a posterior probability of BI/bootstrap value for NJ. Eacc unique sequence is presented witc a GenBank accession no. followed by tce vouccer number, and its cost and locality. Tcree sequences (LT576294-LT576296) were included as reference sequences from Ccilton et al. [12]. Cloacina ernabella was used as tce outgroup. Scale-bar indicates tce number of inferred substitutions per nucleotide site. Abbreviations: NSW, New Soutc Wales; NT, Nortcern Territory; QLD, Queensland; stn, station; VIC, Victoria; WA, Western Australia

Fig. 3 Pharyngostrongylus patriciae n in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 3 Pharyngostrongylus patriciae n. sp. from tce stomacc of Osphranter robustus. 1, Oesopcagus, lateral view; 2, Buccal capsule, lateral view; 3, Buccal capsule and labial crown elements; 4, Oral opening apical view; 5, Bursa, apical view; 6, Spicule tip, ventral view; 7, Female tail, lateral view; 8, Vagina and ovejector, lateral view. Scale-bars: 1, 7, 8, 100 μm; 2–4, 10 μm; 5–6, 50 μm

Fig. 5 in Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp.

Fig. 5 Pcylogenetic analysis of tce ITS2 rDNA sequences of Pharyngostrongylus kappa and Pharyngostrongylus patriciae n. sp. from various cost species and geograpcical locations. Tce sequence data were analysed using tce Neigcbour-Joining (NJ) and Bayesian Inference (BI) metcods. Tcere was a concordance between tce topology of tce BI tree and tce NJ tree (not scown). Nodal support is given as a posterior probability of BI/bootstrap value for NJ. Eacc unique sequence is presented witc a GenBank accession no. followed by tce vouccer number, and its cost and locality. Tcree sequences (LT576294-LT576296) were included as reference sequences from Ccilton et al. [12]. Cloacina ernabella was used as tce outgroup. Scale-bar indicates tce number of inferred substitutions per nucleotide site. Abbreviations: NSW, New Soutc Wales; NT, Nortcern Territory; QLD, Queensland; stn, station; VIC, Victoria; WA, Western Australia

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Fig. 3 in Molecular and Morphological Snapshot Characterisation of the Protist Communities in Contrasting Alpine Glacier Forefields

Fig. 3. Rarefaction analysis derived from the clone libraries of the vegetated transects of Tiefen forefield and Wildstrubel forefield. Dashed lines correspond to 95% confidence intervals.

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Fig. 2 in Molecular and Morphological Snapshot Characterisation of the Protist Communities in Contrasting Alpine Glacier Forefields

Fig. 2. Relative abundances (in percentage) of ciliate-related sequences detected in the 18S rRNA gene clone libraries from the vegetated transects of the (a) Tiefen forefield and (b) Wildstrubel forefield. Species names are based on BLAST comparison of the sequences with the NCBI database (first similarity with a known taxonomic group).

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Fig. 1 in Molecular and Morphological Snapshot Characterisation of the Protist Communities in Contrasting Alpine Glacier Forefields

Fig. 1. Location of the two sampled forefields of the (a) Tiefen glacier and (b) Wildstrubel glacier. Dots indicate sampling spots (white: unvegetated transects; black: vegetated transects).

Fig. 2 in Tegumental topography and molecular characterisation of two trematodes (Platyhelminthes: Digenea) from Clarias gariepinus (Burchell, 1822) in Kenya

Fig. 2. Scanning electron micrographs of Glossidium pedatum. (A) Round oral sucker, (B) spine surrounding the oral sucker (C) location of the genital pore and a protruding cirrus, (D) structure of the cirrus sac (abbreviations: pl-papillae like lappet; os-oral sucker; pp-papillae; dp-dome papillae; vs-ventral sucker; gp-genital pore; c -cirrus; rp-rows of small papillae).

Fig. 5 in Tegumental topography and molecular characterisation of two trematodes (Platyhelminthes: Digenea) from Clarias gariepinus (Burchell, 1822) in Kenya

Fig. 5. Phylogenetic relationships of Tylodelphys mashonensis (Sudarikov, 1971) to other Diplostomidae based on cox1. Phylogram was reconstructed using Bayesian Inference (BI) with Diplostomum spathaceum (Rudolphi, 1819) as an outgroup. Nodal values <0.90 (BI) are indicated by dashes (sequences of the present study are highlighted in bold).

Fig. 3 in Tegumental topography and molecular characterisation of two trematodes (Platyhelminthes: Digenea) from Clarias gariepinus (Burchell, 1822) in Kenya

Fig. 3. Phylogenetic relationships of Glossidium pedatum Loos, 1899 to other members of Plagiorchioidea based on 28S rDNA. Phylogram was reconstructed using Bayesian Inference (BI) with Alloglossidium corti (Lamont, 1921) as an outgroup. Nodal values <0.90 (BI) are indicated by dashes (sequences of the present study are highlighted in bold).

Fig. 1 in Tegumental topography and molecular characterisation of two trematodes (Platyhelminthes: Digenea) from Clarias gariepinus (Burchell, 1822) in Kenya

Fig. 1. Geographical location of the study area: A– Kenya shaded on the African continent; B – shows position of Nyandarua County in Kenya; C – indicates the position of the Lake Ol'Bolossat and the sampling sites (S1–S3).

Fig. 4 in Tegumental topography and molecular characterisation of two trematodes (Platyhelminthes: Digenea) from Clarias gariepinus (Burchell, 1822) in Kenya

Fig. 4. Scanning electron micrographs of Tylodelphys mashonensis. (A) ventral surface ultrastructure, (B) sensory papillae surrounding the oral sucker (C) ventral sucker surrounded by small and large papillae, (D) well organized holdfast organ with spines (abbreviations: ps-pseudo suckers, os-oral sucker, ho- holdfast organ, vs-ventral sucker, pp-papillae, sp-symmetrical papillae, lp- large papillae, hf-holdfast fissure).

Fig. 4 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 4. Phylogenetic tree from maximum likelihood analysis of MSP nucleotide sequences of marine and terrestrial mammal parasitic nematodes including GenBank accession numbers. The percentage of replicate trees in which the associated species clustered together in the bootstrap test (1000 replicates) is shown next to the branches.

Fig. 5 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 5. Phylogenetic tree from maximum likelihood analysis of MSP amino acid sequences of marine and terrestrial mammal parasitic nematodes including GenBank accession numbers. The percentage of replicate trees in which the associated species clustered together in the bootstrap test (1000 replicates) is shown next to the branches. *amino acid sequence translated from EST sequences.

Fig. 3 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 3. Alignment of MSP amino acid sequences of marine and terrestrial mammal parasitic nematodes using the Clustal W method.

Fig. 2 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 2. Alignment of MSP nucleotide sequences of marine and terrestrial mammal parasitic nematodes using the Clustal W method.

Fig. 1 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 1. Anthelmintic treatment pattern, blood sampling time points and corresponding OD values of harbour seals in rehabilitation. Harbour seals (n = 6) were treated twice with ivermectin at arrival (day 1) at the Seal Rehabilitation and Research Centre, Pieterburen, The Netherlands, and 21 days later and once with mebendazole between day 2 and 6. OD values in grey boxes show lungworm-ELISA positive serum samples, those highlighted in blue lungworm-ELISA negative samples. Harbour seal individuals highlighted in dark grey coughed up lungworms one day after arrival. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 5 in Prevalence and molecular characterisation of Acanthocephala in pinnipedia of the North and Baltic Seas

Fig. 5. Maximum likelihood tree based on COI sequences using the Jones-TaylorThornton (JTT) model. The log likelihood is −2479.70. The percentage of trees based on 1000 bootstrap replicates in which the associated taxa clustered together is shown next to the branches. GenBank accession numbers of analysed amino acid sequences are listed in Table 2.