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692 results for “molecular diversity”
Supplementary Material to article "Molecular Diversity of Mycobacterium avium subsp. paratuberculosis in Four Dairy Goat Herds from Thuringia (Germany)"
<p>These data (supplementary material) belong to the publication "Molecular Diversity of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in Four Dairy Goat Herds from Thuringia (Germany)". The study determined the diversity of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) isolated from four goat herds affected by paratuberculosis in Thuringia (Germany), as well as the detailed distribution of MAP genotypes among the animals and their environment in one herd (herd 1). A combination of three methods was used to genotype isolates from fecal samples of infected goats, from various intestinal and other tissues of clinically affected goats, and from environmental samples. The six MAP-C genotypes identified could be assigned to five different phylogenetic subgroups. The results suggest individual infection strains within each herd. In herd 1, one predominant strain was found, and two strains occurred sporadically. The identified genotypes were not goat specific.</p>
WS22 database: combining Wigner Sampling and geometry interpolation towards configurationally diverse molecular datasets
<p>The WS22 database provides a collection of molecular datasets that explores a broad configurational space of flexible organic molecules with varying sizes and complexity. It includes several chemical properties calculated with a quantum chemical (QM) method. Complementary to the structured datasets, this repository also provides the molecular geometries for the equilibrium structures together with the corresponding output of the QM frequency calculations. Details about the methodology, content, and structure of the WS22 datasets are provided in the README file included in this repository.</p>
Supplementary data: Agro-morphological and molecular characterization reveal deep insights in promising genetic diversity and marker-trait associations in Fagopyrum esculentum and F. tataricum
<p>Our study focuses on the global/European buckwheat germplasm collected as part of the ECOBREDD project. The potential of this highly diverse collection for organic buckwheat breeding was evaluated at two complementary levels: phenotypic and genetic. Here, we characterized the phenotypic and genetic diversity of a global collection of the two cultivated buckwheat species <em>Fagopyrum esculentum</em> and <em>F. tataricum</em> (190 and 51 accessions, respectively) using 37 agro-morphological traits and 24 SSR markers (Simple Sequence Repeats) (see publication and info sheet of the data).</p>
MCR LTER: Coral Reef: Distinguishing the molecular diversity, nutrient content, and energetic potential of exometabolomes produced by macroalgae and reef-building corals; data for Kelly et al., 2022 PNAS
Metabolites exuded by primary producers comprise a significant fraction of marine dissolved organic matter, a poorly characterized, heterogenous mixture that dictates microbial metabolism and biogeochemical cycling. We present a foundational untargeted molecular analysis of exudates released by coral reef primary producers using liquid chromatography–tandem mass spectrometry to examine compounds produced by two coral species and three types of algae (macroalgae, turfing microalgae, and crustose coralline algae [CCA]) from Mo’orea, French Polynesia. Of 10,568 distinct ion features recovered from reef and mesocosm waters, 1,667 were exuded by producers; the majority (86%) were organism specific, reflecting a clear divide between coral and algal exometabolomes. These data allowed us to examine two tenets of coral reef ecology at the molecular level. First, stoichiometric analyses show a significantly reduced nominal carbon oxidation state of algal exometabolites than coral exometabolites, illustrating one ecological mechanism by which algal phase shifts engender fundamental changes in the biogeochemistry of reef biomes. Second, coral and algal exometabolomes were differentially enriched in organic macronutrients, revealing a mechanism for reef nutrient-recycling. Coral exometabolomes were enriched in diverse sources of nitrogen and phosphorus, including tyrosine derivatives, oleoyl-taurines, and acyl carnitines. Exometabolites of CCA and turf algae were significantly enriched in nitrogen with distinct signals from polyketide macrolactams and alkaloids, respectively. Macroalgal exometabolomes were dominated by nonnitrogenous compounds, including diverse prenol lipids and steroids. This study provides molecular-level insights into biogeochemical cycling on coral reefs and illustrates how changing benthic cover on reefs influences reef water chemistry with implications for microbial metabolism. This material is based upon work supported by the U.S. National Science Founda
Molecular characterization and genetic diversity of four undescribed novel oleaginous Mortierella alpina strains from Libya
<p>A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. <em>Mortierella</em> is one of the significant genera in this field and contains about hundred species. Moreover, <em>M. alpina </em>is the main single cell oil producer / arachidonic acid producer at commercial scale under this genus.</p>
Data from: Drift happens: molecular genetic diversity and differentiation among populations of jewelweed (Impatiens capensis Meerb.) reflect fragmentation of floodplain forests
Landscape features often shape patterns of gene flow and genetic differentiation in plant species. Populations that are small and isolated enough also become subject to genetic drift. We examined patterns of gene flow and differentiation among 12 floodplain populations of the selfing annual jewelweed (Impatiens capensis Meerb.) nested within four river systems and two major watersheds in Wisconsin, USA. Floodplain forests and marshes provide a model system for assessing the effects of habitat fragmentation within agricultural/urban landscapes and for testing whether rivers act to genetically connect dispersed populations. We generated a panel of 12,856 single nucleotide polymorphisms and assessed genetic diversity, differentiation, gene flow, and drift. Clustering methods revealed strong population genetic structure with limited admixture and highly differentiated populations (mean multilocus FST = 0.32, FST' = 0.33). No signals of isolation by geographic distance or environment emerged, but alleles may flow along rivers given that genetic differentiation increased with river distance. Differentiation also increased in populations with fewer private alleles (R2 = 0.51) and higher local inbreeding (R2 = 0.22). Populations varied greatly in levels of local inbreeding (FIS = 0.2 to 0.9) and FIS declined in smaller, more isolated populations. These results suggest that genetic drift dominates other forces in structuring these Impatiens populations. In rapidly changing environments, species must migrate or genetically adapt. Habitat fragmentation limits both processes, potentially compromising the ability of species to persist in fragmented landscapes.
FIGURE 30 in Morphological and molecular data reveal the cryptic diversity among populations of Aegla paulensis (Decapoda, Anomura, Aeglidae), with descriptions of four new species and comments on dispersal routes and conservation status
FIGURE 30. Bayesian tree (TPM 2 uf + G) for Aegla species based on partial fragment of 16 S. Node numbers represent posterior probabilities (values <50 % are not shown), and divergence time in millions of years (my); * indicates the calibration points to molecular clock. The clade C proposed by Pérez-Losada et al. (2004) is highlighted in grey. The basin and sub-basin origin of the discussed species in this study are shown after the specific names.
FIGURE 24. A – L in Morphological and molecular data reveal the cryptic diversity among populations of Aegla paulensis (Decapoda, Anomura, Aeglidae), with descriptions of four new species and comments on dispersal routes and conservation status
FIGURE 24. A – L, proximal portion of fifth pereiopod showing coxa and sexual tube of long and narrow type. A – B, Aegla paulensis Schmitt, 1942 s. str., male topotype (MZUSP 34368). C – D, Aegla rosanae Campos Jr., 1998, male topotype (MZUSP 34369). E – F, Aegla vanini n. sp., male paratype (MZUSP 34372). G – H, Aegla japi n. sp., male paratype (MZUSP 34375). I – J, Aegla jaragua n. sp. male paratype (MZUSP 34378). K-L, Aegla jundiai n. sp., male paratype (MZUSP 13490). Bars: A – D, F – H, J = 200 µm; K, L = 100 µm; E, I = 500 µm.
FIGURE 8 in Morphological and molecular data reveal the cryptic diversity among populations of Aegla paulensis (Decapoda, Anomura, Aeglidae), with descriptions of four new species and comments on dispersal routes and conservation status
FIGURE 8. Types of Aegla Leach, 1820 male sexual tubes. A, long and narrow (A. lancinhas Bond-Buckup & Buckup in Santos et al., 2015, MZUSP 34403). B, short and wide (A. leptochela Bond-Buckup & Buckup, 1994, MZUSP 34491).
FIGURE 1 in Morphological and molecular data reveal the cryptic diversity among populations of Aegla paulensis (Decapoda, Anomura, Aeglidae), with descriptions of four new species and comments on dispersal routes and conservation status
FIGURE 1. Distribution of the species of Aegla in four main hydrographic basins of southern Brazil: Rio Grande, Rio Tietê (Upper Paraná system), Rio Paraíba do Sul and Ribeira de Iguape. Indications L 1 through L 7 refer to the locations mentioned under “ sampling area ” in the Material & Methods section.
FIGURE 15 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 15 Light micrographs of Longidorus pini Andrés and Arias, 1988 paratypes from San Martín del Pimpollar, Avila province (A–F), and the population from Nava de Francia, Salamanca province (G–M). A–C, G–I, female anterior regions. D–F, J–K, female tails. L-M, male tail with detail of spicules. Abbreviations: a = anus; gr = guiding ring; sp = spicules; spl = ventromedian supplements. Scale bars = 20 μm
FIGURE 13 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 13 Light micrographs of Longidorus cf. olegi Kankina & Metlitskaya, 1983. (A)–(B) female anterior region. (C)–(F) female lip regions. (G) detail of basal bulb. (H) vulval region. (I)–(L) female tails. (M) and (N), male tail with detail of spicules. (O)–(R) First-, second-, third-, and fourth-stage juvenile (J1–J4) tails, respectively. Abbreviations: a = anus; af = amphidial fovea; gr = guiding ring. Scale bars = 20 μm
FIGURE 14 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 14 Relationship of body length to length of functional and replacement odontostyle (Ost and rOst, respectively) length in all developmental stages from first-stage juveniles (J1) to mature females of Longidorus cf. olegi Kankina & Metlitskaya, 1983
FIGURE 12 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 12 Light micrographs of Longidorus nevesi Macara 1985. (A) female anterior region. (B) female lip region. (C) vulval region. (D) female tail. (E)–(F) male tail with detail of spicules. Abbreviations: a = anus; gr = guiding ring; spl = ventromedian supplements; V = vulva. Scale bars = 20 μm
Figure 11 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
Figure 11 Light micrographs of Longidorus carpetanensis Arias et al., 1986 from Puebla de Sanabria, Zamora (A)–(F), and topotypes from Navalmoral, Avila (G)–(L). A–C, G and H, female anterior regions. D, I, female tails. E and F, J and L, male tail with detail of spicules. Abbreviations: a = anus; gr = guiding ring; spl = ventromedian supplements. Scale bars = 20 µm
FIGURE 9 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 9 Light micrographs of Longidorus pacensis sp. nov. (A)–(G) Anterior regions. (H) Vulval region. (I)–(K) Female tails. (L) Detail of sperm cells. (M) and (N) Male tails. (O)–(R) First-, second-, third-, and fourth-stage juvenile (J1–J4) tails, respectively. Abbreviations: a = anus; af = amphidial fovea; gr = guiding ring; sp = spicules; spl = ventromedian supplements; v = vulva. Scale bars = 20 μm
FIGURE 10 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 10 Light micrographs of Longidorus africanus Merny, 1966. (A) female anterior region. (B) female lip region. (C) vulval region. (D)–(E) female tails. Abbreviations: a = anus; gr = guiding ring; v = vulva. Scale bars = 20 μm
FIGURE 8 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 8 Line drawings of Longidorus pacensis sp. nov. (A) Female neck region. (B) and (C) Female lip regions. (D) and (E) Female tails. (F) Male tail. (G) First-stage juvenile tail
FIGURE 7 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 7 Relationship of body length to length of functional and replacement odontostyle (Ost and rOst, respectively) length in all developmental stages from first-stage juveniles (J1) to mature females of. (A) Longidorus iliturgiensis sp. nov. (B) Longidorus pacensis, sp. nov.
FIGURE 4 Phylogenetic relationships within the genus Longidorus. Bayesian 50 in Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
FIGURE 4 Phylogenetic relationships within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from cytochrome c oxidase subunit I (CoxI) mtDNA gene sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gammashaped distribution (GTR + I + G). Posterior probabilities greater than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.