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11 results for “nectar yeasts”
Data from: Genetic admixture increases phenotypic diversity in the nectar yeast Metschnikowia reukaufii,
<p>Raw data and supplementary files for the manuscript "Genetic admixture increases phenotypic diversity in the nectar yeast <em>Metschnikowia reukaufii</em>."</p> <p>-------------------</p> <p><strong>Table S5.xlsx </strong>-- Pairwise correlations between phenotypic traits of <em>Metschnikowia reukaufii</em>.</p> <p><strong>Table S6.xlsx</strong> -- Detailed results obtained in tests of phylogenetic signal for different phenotypic traits and indices of overall performance of <em>Metschnikowia reukaufii</em>.</p> <p><strong>Table S7.xlsx</strong> -- Detailed model fitting results obtained for phenotypic traits and indices of overall performance of <em>Metschnikowia reukaufii</em>.</p> <p><strong>mronlyvcf-renamed.vcf</strong> -- High coverage SNPs obtained from whole genome mapping of 73 <em>Metschnikowia reukaufii</em> strains to diploid reference (mean coverage = 47.9×, range 23 – 116×).</p> <p><strong>MR_phenotypes.xlsx</strong> -- Phenotypic data obtained for 73 <em>Metschnikowia reukaufii</em> strains.</p>
Data from: Genetic basis of priority effects: insights from nectar yeast
Priority effects, in which the order of species arrival dictates community assembly, can have a major influence on species diversity, but the genetic basis of priority effects remains unknown. Here, we suggest that nitrogen scavenging genes previously considered responsible for starvation avoidance may drive priority effects by causing rapid resource depletion. Using single-molecule sequencing, we de novo assembled the genome of the nectar-colonizing yeast, Metschnikowia reukaufii, across eight scaffolds and complete mitochondrion, with gap-free coverage over gene spaces. We found a high rate of tandem gene duplication in this genome, enriched for nitrogen metabolism and transport. Both high-capacity amino acid importers, GAP1 and PUT4, present as tandem gene arrays, were highly expressed in synthetic nectar and regulated by the availability and quality of amino acids. In experiments with competitive nectar yeast, Candida rancensis, amino acid addition alleviated suppression of C. rancensis by early arrival of M. reukaufii, corroborating that amino acid scavenging may contribute to priority effects. Because niche pre-emption via rapid resource depletion may underlie priority effects in a broad range of microbial, plant and animal communities, nutrient scavenging genes like the ones we considered here may be broadly relevant to understanding priority effects.
Data from: Genomic diversity of a nectar yeast clusters into metabolically, but not geographically, distinct lineages
Both dispersal limitation and environmental sorting can affect genetic variation in populations, but their contribution remains unclear, particularly in microbes. We sought to determine the contribution of geographic distance (as a proxy for dispersal limitation) and phenotypic traits (as a proxy for environmental sorting), including morphology, metabolic ability, and interspecific competitiveness, to the genotypic diversity in a nectar yeast species, Metschnikowia reukaufii. To measure genotypic diversity, we sequenced the genomes of 102 strains of M. reukaufii isolated from the floral nectar of hummingbird-pollinated shrub, Mimulus aurantiacus, along a 200-km coastline in California. Intraspecific genetic variation showed no detectable relationship with geographic distance, but could be grouped into three distinct lineages that correlated with metabolic ability and interspecific competitiveness. Despite ample evidence for strong competitive interactions within and among nectar yeasts, a full spectrum of the genotypic and phenotypic diversity observed across the 200-km coastline was represented even at a scale as small as 200 m. Furthermore, more competitive strains were not necessarily more abundant. These results suggest that dispersal limitation and environmental sorting might not fully explain intraspecific diversity in this microbe and highlight the need to also consider other ecological factors such as trade-offs, source-sink dynamics, and niche modification.
Data from: Genetic basis of priority effects: insights from nectar yeast
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Data from: Genomic diversity of a nectar yeast clusters into metabolically, but not geographically, distinct lineages
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Two <em>Metschnikowia</em> nectar yeast species have similar volatile profiles, but elicit differential foraging in bee pollinators
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Data from: Consequences of a nectar yeast for pollinator preference and performance
Pollinators utilize floral resources that vary in colour, scent and reward quality. Variation in such traits, including nectar rewards, in addition to cues associated with their quality, can influence pollinator foraging decisions with consequences for pollinator reproductive success. Nectar is commonly subject to colonization by micro-organisms capable of affecting a suite of traits important for pollinator attraction and fitness; yet, links between microbial presence and changes in pollinator preference and performance remain few. Here, we evaluated the effects of a nectar-inhabiting micro-organism on pollinator foraging behaviour and reproduction using the common eastern bumblebee Bombus impatiens and the cosmopolitan nectar yeast Metschnikowia reukaufii. Using a combination of choice and no-choice behavioural and feeding assays, we manipulated the presence and viability of M. reukaufii in nectar and assessed bumblebee foraging and reproductive responses. Bombus impatiens workers responded positively to the presence of yeasts. Foragers trained to associate yeast presence with flower colour visited a significantly greater proportion of flowers inoculated with yeast when subject to a colour discrimination test. Moreover, foragers naïve to nectar yeasts incorporated more yeast-inoculated flowers into initial foraging bouts when presented with a novel floral array. In addition, bees spent significantly longer foraging on yeast-inoculated flowers compared to yeast-free flowers. However, when we manipulated yeast presence and viability in microcolonies of queenless workers, we found no effect of yeast on components of bumblebee reproduction, such as initiation of egg laying and number of eggs laid. This lack of an effect of yeast persisted even under conditions of pollen limitation. Taken together, these results suggest that nectar yeasts can enhance floral signalling and alter pollinator foraging behaviour at individual flowers, though they may not directly affect pollinator performance. Thus, nectar yeasts may play a significant role in mediating pollinator foraging behaviour, with consequences for plant fitness and evolution of floral traits.
Data from: Nectar bacteria, but not yeast, weaken a plant-pollinator mutualism
Mutualistic interactions are often subject to exploitation by species that are not directly involved in the mutualism. Understanding which organisms act as such 'third-party' species and how they do so is a major challenge in the current study of mutualistic interactions. Here, we show that even species that appear ecologically similar can have contrasting effects as third-party species. We experimentally compared the effects of nectar-inhabiting bacteria and yeasts on the strength of a mutualism between a hummingbird-pollinated shrub, Mimulus aurantiacus, and its pollinators. We found that the common bacterium Gluconobacter sp., but not the common yeast Metschnikowia reukaufii, reduced pollination success, seed set and nectar consumption by pollinators, thereby weakening the plant–pollinator mutualism. We also found that the bacteria reduced nectar pH and total sugar concentration more greatly than the yeasts did and that the bacteria decreased glucose concentration and increased fructose concentration whereas the yeasts affected neither. These distinct changes to nectar chemistry may underlie the microbes' contrasting effects on the mutualism. Our results suggest that it is necessary to understand the determinants of microbial species composition in nectar and their differential modification of floral rewards to explain the mutual benefits that plants and pollinators gain from each other.
Data from: Nectar bacteria, but not yeast, weaken a plant-pollinator mutualism
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Data from: Jack of all nectars, master of most: DNA methylation and the epigenetic basis of niche width in a flower-living yeast
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Data from: Consequences of a nectar yeast for pollinator preference and performance
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