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2,582 results for “nitrogen”
Carbon and nitrogen content and stable isotope compositions from biota samples from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Stable isotopic composition can be used to differentiate between predominantly marine and terrestrial food sources in nearshore marine food webs. The Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program employs spatial and temporal sampling regimes to track shifts in diet, both seasonally (ice cover, ice break-up, and open water), and spatially among lagoons along the Alaskan Beaufort Sea coast. Ice coring, net tows, ponar grabs, and trawls are employed to collect organisms associated with the sea-ice interface, the water column, and the benthos respectively. Tissues are analyzed for stable isotopic composition as well as organic carbon and nitrogen content.
Georgia Salt Marsh: Soil Organic Carbon, Nitrogen, Bulk Density, Moisture, and Texture
As part of project predicting soil carbon at depth from that found at the surface using remote sensing, 28 soil cores were taken from six salt marshes along the Georgia coastline. Cores were taken as deep as possible (25 – 165 cm) and sectioned into 5 cm depths. Soils were analyzed for organic carbon (SOC), total nitrogen (N), bulk density (BD), and particle size (by horizon). Stable carbon isotopes were obtained in three marshes on Sapelo Island; a subset was also analyzed for radiocarbon.
Carbon and nitrogen content and stable isotope compositions from particulate organic matter samples from lagoon, river, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for particulate organic carbon (POC) and particulate organic nitrogen (PON) content and stable isotopic composition.
Carbon and nitrogen content and stable isotope composition from sediment organic matter from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple sediment samples from lagoons along the nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Sediment samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for carbon and nitrogen content and stable isotopic composition.
Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) from river, lagoon, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods and analyzed for dissolved organic carbon and total dissolved nitrogen content.
Total dissolved nitrogen (TDN), dissolved organic carbon (DOC), radiocarbon (14C-DOC), and stable carbon (13C-DOC) of surface waters from the Canning River watershed, 2019 and 2021
Sites along the Canning River mainstem and contributing streams near the Kavik River Camp, Alaska, were visited to track changes in stream and river total dissolved nitrogen (TDN) concentration, dissolved organic carbon (DOC) concentration, and the stable carbon (13C) and radiocarbon (14C) isotopic composition of DOC across transitions between the Brooks Range, Brooks foothills, and Arctic Coastal Plain. The dataset also includes water samples collected from lakes, springs, groundwater, and streams and rivers outside the Canning River watershed. Water samples were collected in late April and early August 2019 and in late July and early August 2021. Data include measurements of individual samples for TDN (milligrams nitrogen per liter), DOC (milligrams carbon per liter), carbon-13 of DOC (reported as delta-13C, per mil), carbon-14 of DOC (reported as fraction modern), and analytical error in the fraction modern values. Additional water chemistry data for these samples can be found in Koch et al. (2024). References: Koch, J. C., Connolly, C. T., Repasch, M., Best, H. R., Couvillion, C. S., Hunt, A. (2024). [Dataset] Hydrochemistry and age date tracers from springs, streams, and rivers in the Arctic National Wildlife Refuge, 2019-2022, U.S. Geological Survey data release, https://doi.org/10.5066/P95CXJIT.
Primary producer biomarker profiles of bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA) and their fatty acid (FA) collected from the Beaufort Sea coastal lagoons,2021-2024
Within Stefansson Sound in Prudhoe Bay, AK various organic matter sources were collected to determine multiple biomarker baseline profiles (i.e., bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA), fatty acids (FA)). Some organic matter sources were collected from Elson lagoon in Utqiaġvik, AK and Kaktovik and Jago lagoons in Kaktovik, AK to supplement low sample sizes in some organic matter source groups. Kelp, red algae, terrestrial plants, phytoplankton, and ice algae were collected in 2024 with some supplement samples collected in 2021 - 2023. Stable isotope values of δ13C and δ15N are reported as “del_13c” and “del_15n”, respectively. Individual fatty acids are reported as the percent relative to total fatty acids for 23 fatty acids: C11:0, C12:0, C14:0, C15:1, C15:0, C16:0, C16:1n7, C17:0, C17:1, C18:0, C18:1n9 trans, C18:2n6 cis, C18:1n7, C18:3n3, C20:0, C18:3n6, C20:4n6, C21:0, C22:0, C22:1n9, C23:0, C24:0, C22:6n3. Stable isotope values of δ13C are reported in the following essential amino acids: Valine (Val), Leucine (Leu), iLeu (isoleucine), Methionine (Met), Phenylalanine (Phe). Additionally, we used ice algal diatoms collected in the Arctic (landfast ice near Utqiaġvik, Alaska) and cultured in a laboratory setting at the University of Alaska Fairbanks to compare the CSIA-EAA fingerprints of field (composites) ice algal samples and isolate diatoms samples.
Stormwater Nitrogen in Arizona (SNAZ): runoff and stormwater-mediated export from urbanized catchments within the greater Phoenix metropolitan area, Arizona, USA (2010-2012)
Urbanization alters dramatically watershed ecosystem processes. Land-use change and anthropogenic activities contribute to increased inputs of nutrients and other materials, while changes to land cover alter hydrology and the corresponding movement of materials. These changes have ramifications for both watershed processes and downstream systems. The impacts of urbanization on aquatic systems are well-studied, and frequently encapsulated in the ‘urban stream syndrome’ (Walsh et al. 2005) that describes, among others, increased nutrient loading and stream flashiness. However, there is some evidence that aridland cities behave differently (Grimm et al. 2004, 2005), and the complex dynamics among catchment characteristics, storm attributes, and runoff in highly urbanized settings of the arid Southwest remains poorly understood. To enhance our understanding of stormwater dynamics and watershed functioning in aridland, urban environments, the Central Arizona–Phoenix Long-Term Ecological Research (CAP LTER) program began monitoring stormwater runoff at the outflow of the Indian Bend Wash (IBW) in 2008. The IBW is a tributary to the Salt River in central Arizona, and is a major drainage within the greater Phoenix metropolitan area, encompassing much of the City of Scottsdale. A model of soft engineering, the IBW as it runs through much of the City of Scottsdale is comprised largely of a series of artificial lakes, parks, paths, golf courses, ball fields, and other non-structural elements designed with the dual roles of providing outdoor amenities to the City residents while serving as an effective flood water conveyance feature. A unique biogeochemistry of this novel system is detailed by Roach et al. (2008), and Roach and Grimm (2011). Data and expertise garnered by stormwater monitoring near the outflow of the IBW helped pave the way for a more expansive stormwater research effort facilitated by a leveraged grant from the National Science Foundation (DEB-0918457, NSF Eco
Comparing vertical accretion, organic carbon (C) sequestration, and nitrogen burial between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia.
Restoration of tidal marshes throughout the 20th century have attempted to bring back important functions of natural tidal systems. In this study, vertical accretion, organic carbon (C) sequestration, and nitrogen burial were compared between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia to examine the impacts of restoration years later. On Sapelo Island there are two marshes near the University of Georgia Marine Institute, one of which is a natural marsh, and one of which is a restored marsh. The restored marsh had been diked in 1948, and the dike was breached, allowing for the marsh to be restored, in 1956. Soil cores were collected from both marshes, and the sediments were analysed for Nitrogen and Carbon concentrations and bulk density. This analysis was used to determine accretion rates for the two marshes as well as changes in the restored marsh since the dike was breached. Nitrogen burial, carbon sequestration, and soil accretion in the restored marsh as compared to the natural marsh were the focus of this study.
Organic and Inorganic Nitrogen Uptake by Sarracenia Purpurea at Harvard Forest and Fort Albany ON 2007
The nitrogen-limited carnivorous pitcher plant Sarracenia purpurea and its associated detritus-based food web is a model system for studying plant nutrient dynamics. We tested if S. purpurea can directly take up intact amino acids and compared uptake of organic and inorganic forms of nitrogen (N) across a gradient of N deposition. At sites in Canada and the United States, individual pitchers with complete or incomplete food webs were fed U-13C-15N-glycine, U-13C-15N-phenylalanine and 15NH415NO3 individually and in mixture. Plants took up intact amino acids. Acquisition of each N form provided in isolation exceeded uptake of the same form in mixture. At the high deposition site, uptake of 15N from amino acids was higher than uptake of 15N from inorganic nitrogen. At the low deposition site, uptake of 15N from all three forms of N were similar. Completeness of the associated food web had no effect on 15N uptake. By taking up intact amino acids, Sarracenia purpurea can short-circuit the inorganic N cycle, thus minimizing potential bottlenecks in N availability that result from the plant’s reliance for nitrogen mineralization on a seasonally reconstructed food web operating on infrequent and irregular prey capture.
20-Year Root Mass in Chronic Nitrogen Amendment Experiment at Harvard Forest 2008
Forests typically respond to nitrogen additions with increased productivity, hence a long-held paradigm was that chronic additions of anthropogenically-derived atmospheric deposition would have positive ecosystem effects. However, twenty years of work at the Harvard Forest Chronic N Deposition plots, and in other forest ecosystems as well, has shown that that this simplistic view is incomplete. For example, enhanced ammonium uptake increases soil acidity, leading to mobilization of aluminum, and loss of nutrient cations (e.g. Mg2+, Ca2+, and K+ ) all of which influence root mass, turnover, and activity. To address long-term impacts of N additions on forest root mass, we removed O-horizon (forest floor) samples from the Chronic N hardwood and red pine stands to quantify the total mass of roots. We found that long term N additions had contrasting results in the two forests. In the hardwood plots, total root mass (less than 2mm) increased from 0.167 + 0.026 (S.E.) kg m-2 in the control plot to 0.434 + 0.170 kg m-2 in the high N plots. In contrast, in the red pine stand, roots declined from 0.074 + 0.011 (S.E.) kg m-2 in the control plot to 0.031 + 0.016 kg m-2 in the high N plots. These data are in agreement with data for aboveground productivity at the Chronic N plots, which show stimulated growth in the hardwoods and severe growth declines and enhanced mortality in the pine stand.
Carbon and Nitrogen Across Two ULTRA-Ex Urban to Rural Gradients in Massachusetts 2010
As part of the Boston University-led, Urban Long-Term Research Area - Exploratory Award (ULTRA-Ex), we established 135 circular, 15 m radius biometric plots extending across two Boston urban-to-rural gradients (Boston MA to Petersham MA and Boston MA to Worcester MA). The plots were stratified based on neighborhood (1 km2 surrounding area) characteristics for population density, impervious surface area fraction, and land cover. Within each plot we measured aboveground live and dead biomass, species characteristics, ground cover characteristics, and soil properties.
Litter Decomposition in Response to Nitrogen Addition and Soil Warming at Harvard Forest 2010-2012
The purpose of this study is to examine whether two environmental change stressors (warming and nitrogen deposition) differentially impact litter decomposition. We investigated this using a two year litterbag decomposition experiment at the chronic N amendment experiment and the Barre Woods Soil warming experiment, and measured litter decay dynamics, enzyme activities and litter chemistry. In both years mass loss of the mixed litter was suppressed under N addition, with most of the mass loss observed in the first year compared to the second year (70% and 30% of total mass loss, respectively). Both years showed either increased activity for some hydrolytic enzymes (e.g. cellobiohydrolase) or no difference (e.g. ß-N-acetylglucosaminidase) with increased N. The lignolytic enzymes (e.g. peroxidases) showed no difference in activity in the first year, but had a highly reduced activity in year 2 under elevated N conditions. Soil warming did not significantly affect litter mass loss, and only had an effect on the activity of a few enzymes. In the oak reciprocal litterbag study, decay of oak litter originating from the highest N addition plot was negatively affected by simulated N deposition in the first year of decomposition, while after two years, simulated N deposition negatively affected all litter, and litter originating from the highest N addition plot decayed more slowly than control litter even without added N (i.e. in the control plot). In addition, in the first year of decomposition lignolytic enzyme activities were suppressed in litter originating from the N addition treatments, but due to simulated N deposition in year two.
Foliar and Soil Chemistry at Harvard Forest Chronic Nitrogen Amendment Experiment 1995-2009
The aim of the chronic N study at Harvard Forest is to increase our understanding of ecosystem nitrogen dynamics in response to elevated nitrogen inputs. In recent, nitrogen deposition in the Northeastern United States has been 10 to 20 times above historic background levels which could possibly saturate the retention capacity of a forest ecosystem. Long-term elevated N deposition typically leads to an increase in the concentration of total foliar N, with or without similar changes in the important base elements such as Ca, Mg and K. This increase in leaf N content also leads to significant shifts in the internal partitioning of N within the leaf. For example, in conifers, N deposition has been shown to significantly increase leaf N present in the form of free amino acids such as arginine. Little is known about N partitioning for hardwoods under these conditions. These changes in N partitioning are possibly connected to leaf function. The present study was conducted to experimentally test whether the alterations in N partitioning do occur due to long-term N deposition and if so do they have a positive or a negative effect on photosynthetic capacity and biomass production. A possible decoupling of the relationship between foliar N and photosynthetic rate may occurs under these conditions. The treatment plots used in this study are part of the Chronic Nitrogen Amendment Study at the Harvard Forest LTER site (42.5°N, 72°W). The site has a temperate climate with monthly temperatures ranging from -7°C in January to 20°C in July. Average annual precipitation is 110 cm (http://harvardforest.fas.harvard.edu). The site averages approximately 8 kg ha-1 year-1 of total N deposition. As reported earlier, the land-use history of the pine and hardwood stands used in this study is very different. Two adjacent stands were chosen for the study: an even-aged red pine (Pinus resinosa Ait.) stand and a 50-year-old mixed hardwood stand that had regenerated naturally after clearcutting i
Soil Carbon and Nitrogen at the Harvard Farm at Harvard Forest since 2015
These data represent baseline soil bulk density and total soil carbon and nitrogen concentrations for the three grazing treatments at Harvard Farm: hay (no grazing), rotational grazing, and intensive grazing. These samples were collected at the beginning of the study, so any differences across plots or treatments are due to inherent soil variability at the site rather than the treatments themselves. These data were collected to establish the starting conditions with which to compare potential treatment effects at later sampling dates.
Leaf and Soil Nitrogen Following Lymantria dispar Defoliation in Central Massachusetts 2018-2019
In this study we investigated relationships between ecosystem nitrogen (N) cycling and tree defoliation during a recent 2015-2018 irruption of invasive Lymantria dispar caterpillars, which can cause tree stress and sometimes mortality following multiple years of defoliation. Nitrogen is a critical nutrient that limits the growth of caterpillars and plants in temperate forests. We assessed the associations between N concentrations, soil solution inorganic N availability, and defoliation intensity by L. dispar at the scale of individual trees in the Amherst, MA area and forest plots in the Quabbin Reservoir area.
Soil Warming Plus Nitrogen Addition Experiment at Harvard Forest since 2006
Climate warming and N deposition are occurring on a global scale with unknown long-term effects on soil microbial communities and the biogeochemical processes they perform. Few studies have examined the interactive effects of elevated temperatures and N additions on soil microbial community structure and function. The overall objective of this study is to investigate whether warming and N additions restructure microbial communities and alter the response of soil C pools to these two stressors. A related study is examining the interactive effects of warming and N additions on plant and ant diversity. This research is being carried out at the Soil Warming x Nitrogen Addition Study at the Harvard Forest which includes four treatments: control, warming (heating to 5 deg C above ambient), warming x N, and N additions only (addition of 50 kg N/ha/yr). Soil respiration measurements have been made monthly since the beginning of the experiment in 2006. In 2010 and 2011, two different methods were compared: static chamber measurements and instantaneous field IRGA assessments. Soil samples (~0-10 cm) have been sampled annually for total C and N, N mineralization, and microbial community composition. Most recently, soils were collected in October 2011 from across the entire profile (0-50 cm) to access potential changes in soil C and N pools with depth. First, 20 x 20 cm forest floor samples were collected. Mineral soils were then collected in 10 cm depth increments to ~50 cm. Samples are currently being analyzed for total C and N, microbial biomass and community composition and fungal gene expression (transcriptomics). Additionally, long-term incubations are being conducted to measure labile and recalcitrant C fractions. Additional soil physical (texture) and chemical (pH, inorganic N) are being measured. Field season measurements of soil respiration indicate that both warming and N additions continue to stimulate CO2 flux, with warming treatments having a stronger effect on re
Chronic Nitrogen Amendment Experiment at Harvard Forest since 1988
The purpose of this study is to increase our understanding of ecosystem nitrogen dynamics in response to elevated nitrogen inputs. With atmospheric nitrogen deposition in the Northeastern United States currently at 10 to 20 times above historic background levels, it is possible that excessive nitrogen inputs could saturate the retention capacity of a forest ecosystem. Potential effects of nitrogen saturation include increased nitrate leaching and simultaneous base cation losses, soil acidification, altered fluxes of trace gases and forest decline. Two adjacent stands were chosen for the study: an even-aged red pine (Pinus resinosa Ait.) stand planted in 1926 and a 50-year-old mixed hardwood stand that had regenerated naturally after clearcutting in approximately 1945. The hardwood stand is dominated by black and red oak (Quercus velutina Lam.; Q. rubra L.) with significant amounts of black birch (Betula lenta L.), red maple (Acer rubrum L.) and American beech (Fagus grandifolia Ehrh.). The dominant soil types are stony- to sandy-loams formed from glacial till, and are classified as Typic Dystrochrepts of the Canton or Montauk series. Four treated plots were established within each stand: control, low N, low N plus sulfur (N+S) and high N. Each plot measures 30 x 30 meters (0.09 ha) and is divided into thirty-six 5 x 5 m subplots.
Nitrogen Cycling Dynamics in Sarracenia Purpurea at Harvard Forest 2004-2005
In nutrient poor systems, plants employ many strategies in order to acquire and recycle scarce nutrients, including nitrogen. Low leaf N content is associated with low photosynthetic rates, but carnivorous plants have unusually low photosynthetic rates given their N content. The northern pitcher plant Sarracenia purpurea readily uses any available nitrogen: NH4 and NO3 dissolved in precipitation; N mineralized from captured prey; the scant N in saturated peat; and N remobilized from storage. However, the dynamics of N cycling within S. purpurea are poorly understood. We conducted two greenhouse experiments to examine N-cycling dynamics of S. purpurea at the whole-plant and individual-leaf levels. In the first experiment we assessed assimilation, translocation, storage, and remobilization of 15N supplied to pitchers and roots. In the second experiment, we examined how 15N assimilated by the first pitcher produced at the start of the growing season contributed to the production and maintenance of subsequent pitchers, roots, and rhizomes. Patterns of N cycling were similar at the individual-leaf and whole-plant level. Pitchers assimilated 55 - 69% of available 15N and served both as the largest sink for newly assimilated N (more than 90% of the 15N assimilated during 2004) and the largest source of N remobilization the following spring. In contrast, N assimilated by roots was low and accounted for less than 2.5% of the overall S. purpurea N budget. S. purpurea uses both stored N and newly-acquired N throughout the growing season. The importance of stored N decreases throughout the growing season as newly assimilated N contributes more to later pitcher production.
Leaf spectroscopy and active fluorescence datasets for early drought and nitrogen stress diagnosis in tomato
<p>The dataset contains different plant physiological parameters collected during a 14-day stress and recovery experiment on tomato (<em>Solanum lycopersicum</em> L. cv Moneymaker) plants, undergoing a nitrogen deficiency, drought or control treatment. </p> <p>A full description of the experiment, together with the scientific results, is published by Pescador-Dionisio et al. (2024), and can be found through: <a href="https://doi.org/10.1111/nph.20253">https://doi.org/10.1111/nph.20253.</a></p> <p>The goal of the dataset collection was to obtain a non-invasive proximal sensing dataset at leaf level (reflectance, transmittance, upward and downward fluorescence), in parallel to gas exchange and active fluorescence measurements. The leaf spectroscopy dataset was further processed by a pigment spectral unmixing algorithm according to Van Wittenberghe et al. (2024), to calculate fluorescence quantum efficiency (<em><strong>FQE</strong></em>) and effective absorbance (<strong><em>A_eff</em></strong>) changes associated to the activation of regulated heat dissipation (<strong><em>A_eff_535_Xan</em></strong>). The latter absorption feature is linked to the xanthophyll ('<strong>Xan</strong>') absorption in the 500-600 nm range, which is modelled by the sum of three Gaussians. For a full description of this feature, see Van Wittenberghe et al. (2021).</p> <p>Gas exchange and active fluorescence measurements were carried out with a LI-6400 portable photosysthesis system (LI-COR Biosciences, Lincoln, USA) equipped with a 6400-40 leaf chamber fluorometer. Steady-state measurements were done at 300 and 1000 μmol m−2 s−1 ('<strong><em>PAR300</em></strong>' and '<em><strong>PAR1000</strong></em>'), i.e. growing light conditions and light saturating conditions. Light response curves were taken on different days. Common fluorescence parameters (e.g., <em><strong>Fv/Fm, Fo, Fm, NPQ, YNO, YNPQ</strong></em>) are provided together with 'sustained' and reversible' NPQ parameters calculated according Porcar-Castell (2011).</p> <p>Leaf spectroscopy and active steady-state fluorescence measurements were performed on the same measuring days ('<em><strong>d0</strong></em>', '<em><strong>d2</strong></em>', '<em><strong>d4</strong></em>', '<em><strong>d7</strong></em>', '<em><strong>d14</strong></em>') and on the same leaf, both at 300 and 1000 μmol m−2 s−1 ('<em><strong>PAR300</strong></em>' and '<em><strong>PAR1000</strong></em>'), taking into account an adaptation time. We used a LED light source and several filters, placed in front of a FluoWat leaf clip, which was connected to two high-performance VIS-NIR spectroradiometers (QEPRO, Ocean Insight Inc., Orlando, Florida, USA). The spectroscopy measurements are presented in the Matlab structures for each measuring day, e.g. "<strong><em>2023_d0_Leaf_Spec_Tomato_Stress.mat</em></strong>".</p> <p>The outputs of the pigment spectral fitting code are presented by Matlab structures, e.g. "<strong><em>2023_d0_Leaf_Fitting_Tomato_Stress.mat</em></strong>", which contains the effective absorbance fitting (<strong><em>A_eff</em></strong>) of each pigment (<strong>Chl a, Chl b, Carotene-b, Anthocyanins, and Xanthophylls</strong>) for the wavelength range [500-780] nm, the absorbed photosynthetically active radiation by Chlorophyll a ('<em><strong>APAR_Chla</strong></em>') for the wavelength range [400-800] nm, and the fluorescence quantum efficiency, calculated as the ratio of the emitted fluorescence photons and the flux of photons absorbed by Chlorophyll a. </p> <p>Additional metadata from HPLC photosynthetic pigment analyses, xanthophyll-related enzyme expression, biomass and total content of elemental nitrogen are provided.</p> <p>Please follow the README files for more detailed information.</p> <p> </p>
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