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45 results for “nitrogen content”
Carbon and nitrogen content and stable isotope compositions from biota samples from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Stable isotopic composition can be used to differentiate between predominantly marine and terrestrial food sources in nearshore marine food webs. The Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program employs spatial and temporal sampling regimes to track shifts in diet, both seasonally (ice cover, ice break-up, and open water), and spatially among lagoons along the Alaskan Beaufort Sea coast. Ice coring, net tows, ponar grabs, and trawls are employed to collect organisms associated with the sea-ice interface, the water column, and the benthos respectively. Tissues are analyzed for stable isotopic composition as well as organic carbon and nitrogen content.
Carbon and nitrogen content and stable isotope compositions from particulate organic matter samples from lagoon, river, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for particulate organic carbon (POC) and particulate organic nitrogen (PON) content and stable isotopic composition.
Carbon and nitrogen content and stable isotope composition from sediment organic matter from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple sediment samples from lagoons along the nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Sediment samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for carbon and nitrogen content and stable isotopic composition.
Soil and foliar carbon and nitrogen content and stable isotope ratios from rainfall manipulation experiments at the Jornada Basin LTER, 2011-2020
As rainfall extremes are expected to increase in novel magnitude and frequency, especially in dryland regions, we asked how prolonged and directional shifts to water availability may affect ecosystem carbon and nitrogen dynamics. This data set includes foliar and soil carbon and nitrogen stable isotope and concentration data collected from multiple long-term rainfall manipulation experiments at the Jornada Basin LTER. Datasets also include rainfall data adjusted to rainfall manipulation intensities. Collection dates range from 5 to 14 years since the onset of experimental treatments. The primary plant species targeted for this study were the dominant grass, Bouteloua eriopoda, and the dominant shrub, Prosopis glandulosa.
Eriophorum vaginatum tiller nitrogen content at the 2007 Anaktuvuk River fire scar and nearby unburned tundra measured in 2019.
Tillers from 24 Eriophorum vaginatum individuals were sampled in late July 2019 to examine differences in percent nitrogen between previously burned (Anaktuvuk River Fire) and unburned tussocks at a nearby unburned control site. At the burned site tussocks exhibiting evidence of rodent grazing were also sampled to separate herbivore effects from those of the fire. From each tussock, 3-4 new leaves were sampled (as indicated by lack of brown tips) and dried at 60°C for 24 hours, before being ground and analyzed for percent nitrogen. The goals of the project were to examine the impact of post-fire changes in plant community composition, nutrient quality and structure on habitat suitability and rodent herbivore activity in response to a large, severe, and unprecedented fire in northern Alaska moist acidic tundra.
Eriophorum vaginatum rhizome nitrogen content from the 2007 Anaktuvuk River fire scar measured in 2019.
This file contains Eriophorum vaginatum rhizome biomass from a 2017 biomass pluck of previously burned tundra (2007 Anaktuvuk River Fire) and nearby unburned tundra. Rhizome biomass from the pluck was combined with rhizome percent nitrogen estimates (2.47% at the Anaktuvuk River Fire, and 1.05% at the nearby unburned site) to estimate grams of nitrogen per meter squared, to evaluate differences in winter forage quality for the rodent herbivore, Microtus oeconomus. Percent nitrogen estimates were derived from pooled rhizome samples collected from the two sites in late 2018. The goals of the project were to examine the impact of post-fire changes in plant community composition, nutrient quality and structure on habitat suitability and rodent herbivore activity in response to a large, severe, and unprecedented fire in northern Alaska moist acidic tundra.
Fire Self-Limitation (FiSL) Experiment: Quantifying Wildfire Carbon Combustion Losses in boreal Deciduous and Mixed Forests in Interior Alaska and the Boreal Cordillera IV: Organic Soil Carbon and Nitrogen Content from Organic Soil Samples 2022
This dataset contains lab-quantified (and some field-measured) characteristics for post-fire residual organic soil samples collected in the field for plots in 8 fire scars in Interior Alaska and the Yukon. Data was collected in the summer of 2022. Fire scars sampled included Shovel Creek (2019), Aggie Creek (2015), Hess Creek (2019), Baker (2015), Munson Creek (2021), Isom Creek (2020), 2019MA014 (2019), and 2019BC005 (2019). Lab analyses were conducted in summer and fall of 2022 at UAF and NAU.
Plant Carbon and Nitrogen Content at the Kellogg Biological Station, Hickory Corners, MI (1989 to 2018)
Dataset Abstract This dataset includes total carbon and total nitrogen measured for plants from the LTER Main Site and the Successional and Forested sites. Total C and N are analyzed at peak biomass for a given treatment. In some systems with multiple harvests or complex communities that have peaks occurring at different times of the year, measurements are taken at multiple times per year. Samples are combusted and measured on a CHN analyzer. original data source http://lter.kbs.msu.edu/datasets/32
Stable isotope content (carbon, nitrogen) for epibenthic suspension feeders and Macrocyctis pyrifera at in the Santa Barbara Channel, 2010-2011
This dataset includes measurments of stable carbon, nitrogen isotopes in tissues of eight epibenthic suspension feeders and Macrocystis pyrifera (giant kelp) at three reefs in the Santa Barbara Channel during 2010 and 2011, as part of a study to (a) determine the contributions of phytoplankton and giant kelp detritus to the pool of suspended reef particulate organic matter (POM), and usage by benthic susupension feeders and (b) whether POM composition varies with distance from kelp forests. Species sampled include Chaceia ovoidea (bivalve), Aglaophenia spp (ostrich plume hydrozoan), Cucumaria piperata (echinoderm), Muricea californica (cnidarian), Phragmatopoma californica (tube worm), Parapholas californica (bivalve), Pachythyone rubra (echinoderm), and Styela montereyensis (tunicate). Samples were collected at three core SBC LTER research reefs: Arroyo Quemado, Arroyo Burro, and Mohawk. Potentially important food sources to benthic suspension-feeders on shallow subtidal reefs include phytoplankton-dominated seston, kelp-derived detritus, and for locations adjacent to sources of freshwater runoff, terrestrially-derived material. Our data will be input to mixing models to estimate the contribution of each source to the reef food web.
Isotopes and content of carbon, nitrogen, and sulfur of eelgrass (Zostera marina) from West Falmouth Harbor from 2005 through 2019
West Falmouth Harbor (West Falmouth, MA, USA) has been experiencing a dramatic increase in nitrogen loading from an upgradient municipal wastewater treatment facility since the early 2000’s. As part of a long-term study into the effects of this nitrogen enrichment, we have been assessing changes in the extent and health of the eelgrass (Zostera marina) community within the harbor. Eelgrass shoots were collected during the summer and run for carbon (C), nitrogen (N), and sulfur (S) content and isotopic composition. Samples were analyzed from 7 stations in the more well-flushed outer harbor (OH), 4 stations in the middle of the harbor (MH), and 6 stations in the inner portion of the harbor in close proximity to a high groundwater nitrate source (Snug Harbor, SH). Carbon data is available from 2011 through 2018, nitrogen data from 2006 through 2018, and sulfur data from 2005 through 2019 with a few samples from 2021. Sulfur results have been published in Haviland et al. 2022 (doi: 10.1002/lno.12025).
Tanana River Floodplain Dissolved Organic Nitrogen (DON) Budget, extracted soil protein content
Ammonium, Nitrate, and Amino Acid concentrations in .5M K2SO4 extracted T0 Tanana Floodplain soils Ammonium, Nitrate, and Amino Acid concentrations in .5M K2SO4 extracted T30 Tanana Floodplain soils Sodium Bicarbonate Extracted Tanana River Floodplain Soil Protein Concentrations, 2001 Plot locations for the Tanana River Floodplain DON buget study. GPS coordinates for the Tanana River Floodplain Dissolved Organic Nitrogen (DON) Budget Study plots All values given are in micrograms of bovine serum albumin equivalents per gram dry weight. Site designations in parenthesis are specific to the study and represent individual transects,each within a unique stand of the following standtypes: W=willow,A=alder,BP=balsam poplar,4=white spruce,5=black spruce. Months: 6=June,7=July,etc.
Nitrogen cycling at treeline. III. Total Soil Carbon and Nitrogen Content
We studied spatial and temporal patterns of nitrogen pools and fluxes in soils at treeline and forested sites within three mountain ranges across a 785 km transect in Alaska during 2001- 2002. We measured pools of soil mineral (ammonium and nitrate) and organic (amino acid and microbial biomass) nitrogen, in situ rates of net mineralization, net nitrification, net amino acid production, and decomposition, as well as soil carbon turnover in a laboratory incubation experiment. A complete characterization of the study can be found in Loomis et al. (2006).
Forest floor carbon and nitrogen content from 9 Hillslope Project sites in Macon County, North Carolina, within the Upper Little Tennessee River Basin
Forest floor samples were collected at 9 hillslope sites representing a gradient of development, including forested, valley agriculture, and mountain housing developments in Macon County, NC. Forest floor was divided into two categories: forest floor (i.e., Oi, Oe, & Oa layers) and wood <10 cm diameter. Afterwards, the combined depth of the Oe and Oa was measured in the middle of 3 sides of the subplot. Forest floor collections were later dried and weighed before being processed for C and N analyses.
Soil water content under rainfall manipulation and nitrogen fertilization treatments at the Jornada Basin LTER site, 2007-2009
This data package contains soil water content data from a precipitation and nitrogen manipulation experiment conducted on the Jornada Experimental Range from 2006-2009. The objective of the study was to understand the interaction of precipitation and nitrogen dynamics on ANPP legacies. Rain-out shelters were used to create 5 levels of precipitation: 80% reduced, 50% reduced, ambient control, 50% increased, and 80% increased precipitation. Half of the plots were randomly assigned to receive ammonium nitrate fertilizer. In a subset of the plots, volumetric soil water content was monitored at shallow (0-5 cm) and deep (30-50 cm) depths. This data set contains the date of collection, block number, plot number, 2007-2008 precipitation treatment, 2009 precipitation treatment, nitrogen treatment, depth of soil probe, sensor voltage, and volumetric water content. Monitoring of soil moisture for this study was discontinued in November 2009. Also available are plant cover data from this study in data package knb-lter-jrn.210278002. For further information and results, see: Throop, H., L. G. Reichmann, O. Sala, and S. Archer. (2012), Response of dominant grass and shrub species to water manipulation: an ecophysical basis for shrub invasion in a Chihuahuan desert grassland. Oecologia 169: 373-383. https://doi.org/10.1007/s00442-011-2217-4 Reichmann, L. G., O. E. Sala, and D. P. C. Peters. (2013), Water controls on nitrogen transformations and stocks in an arid ecosystem. Ecosphere 4(1):11. https://doi.org/10.1890/ES12-00263.1
Nitrogen and phosphorus content of mesquite tree tissues from four habitats at the Jornada Basin LTER site, 1986
This data package contains data on measured concentrations of nutrients in mesquite tree tissues collected from four habitat types at the Jornada Basin LTER site. These habitat types include: (1)playa, (2)arroyo,(3)dunes, and (4)grassland and samples were collected in conjunction with phenology studies and natural abundance of N15 sampling during 1986. Leaves, stems, flowers, and pods were collected from mesquite sample tissues and individually digested and analyzed for total nitrogen (Kjeldahl) and total phosphorus. This data set consists of the date of collection, collection site, leaf total nitrogen, stem total nitrogen, flower total nitrogen, pod total nitrogen, leaf total phosphorus, stem total phosphorus, flower total phosphorus, and pod total phosphorus. Data from this study was collected in 1986. Further data was collected in 1987, but had differing attributes and is therefore published in a separate data package (knb-lter-jrn.210386009).
Nitrogen and phosphorus content of mesquite tree tissues from four habitats at the Jornada Basin LTER site, 1987
This data package contains data on measured concentrations of nutrients in mesquite tree tissues collected from four habitat types at the Jornada Basin LTER site. These habitat types include: (1)playa, (2)arroyo,(3)dunes, and (4)grassland and samples were collected in conjunction with phenology studies and natural abundance of N15 sampling during 1987. Leaves, stems, flowers, and pods were collected from mesquite sample tissues and individually digested and analyzed for total nitrogen (Kjeldahl) and total phosphorus. This data set consists of the date of collection, collection site, leaf total nitrogen, stem total nitrogen, flower total nitrogen, pod total nitrogen, leaf total phosphorus, stem total phosphorus, flower total phosphorus, and pod total phosphorus. Data from this study was collected in 1987. Prior data was collected in 1986, but had differing attributes and is therefore published in a separate data package (knb-lter-jrn.210386008).
SBC LTER: Reef: Macrocystis pyrifera CHN content (carbon, hydrogen, nitrogen), ongoing since 2002
The purpose of monthly kelp CHN sampling is to track the carbon, hydrogen, and nitrogen concentrations in Macrocystis pyrifera tissue at SBC LTER sites over time. A total of fifteen M. pyrifera blades are collected at each of three reef sites once a month. Blades are selected approxiamtely two meters from the growing end of several different plants. Core samples of the blades are processed and run through an Organic elemental analyzer with a combustion method. See Methods for more information.
Effect of shade and nitrogen content on Arabidopsis Col-0 and cytokinin mutants abcg14 and cypDM mRNA-seq gene expression processed tables.
<p>This dataset is an add-on for Gautrat et al., containing processed files for the mRNAseq data in tab delimited txt format.</p> <p>Here, you can obtain the raw counts file, the normalized CPM values, and the normalized logCPM values</p> <p>The RNA-seq raw data supporting the conclusions of this article have been deposited in ArrayExpress (Kolesnikov et al., 2015) at EMBL-EBI (www.ebi.ac.uk/arrayexpress), under accession numbers E-MTAB-13638.</p> <p>All relevant custom r scripts are available at https://github.com/aromanowski/shade_N_ck</p>
Raw data: High-throughput screening of soybean di-nitrogen fixation and seed nitrogen content using spectral sensing
<p>Symbiotic di-nitrogen fixation of grain legumes has a substantial impact on crop performance, harvest product quality, and nitrogen (N) balance of crop rotations, particularly under organic management regimes. In soybean breeding, selection for increased nitrogen fixation is desirable for improving seed protein content and N balance of cropping systems. However, the lack of high-throughput screening methods for direct measurement of N 2 fixation rates prohibits practical breeding efforts. Therefore, hyperspectral canopy reflectance measurement as a field-based phenotyping method was evaluated in three environments for indirect estimation of N fixation and uptake of soil nitrogen in a set of early maturity soybean genotypes exhibiting a wide range in seed protein content. Reflectance spectra were collected in repeated measurements during flowering and early seed filling stages. Subsequently, various spectral reflectance indices (SRIs) were calculated for characterizing nitrogen accumulation of individual genotypes. Moreover, prediction models for seed protein content as an end-of-season target trait were developed utilizing full spectral information in partial-least-square regression (PLSR) models. A number of N-related SRIs calculated from spectral reflectance data recorded at the beginning of the seed filling stage were significantly correlated to seed protein content. The best prediction of seed protein content, however, was achieved in PLSR models (validation R 2 =0.805 across all three environments). Environments lower in initial soil mineral N content appeared as more favorable selection sites in terms of prediction accuracy, because N fixation is not masked by soil N uptake in such environments. Hyperspectral reflectance data proved to be a valuable method for determining genetic variation in crop N accumulation, which might be implemented in high-throughput screening protocols for N fixation in plant breeding programs.</p>
Figure 1 in Soil mineral nitrogen content is increased by soil mesofauna and nematodes - a meta-analysis
Figure 1. Overall effect of the presence of soil (micro- and/or meso) fauna ('All', purple), as well as differentiated by size classes (blue: microfauna, red: mesofauna, orange: micro- and mesofauna) on soil mineral nitrogen compounds. Shown are the mean effect sizes (logarithm of the response ratio), 95 % confidence intervals, and the number of observations (within parentheses). Asterisks indicate levels of significance (* P = 0.05, ** P = 0.01, *** P <0.001).
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