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17 results for “nuclear DNA content”
FIG. 3 in Intraindividual variation in nuclear DNA content in Durvillaea antarctica (Chamisso) Hariot, Macrocystis pyrifera (Linnaeus) C. Agardh and Lessonia spicata (Suhr) Santelices (Phaeophyceae)
FIG. 3. — Developmental stages of sporangia in Macrocystis pyrifera (Linnaeus) C. Agardh stained with DAPI: A-C, four-nucleate sporangium (4-ns) and sporangial mother cells (smc); D, mature sporangia. Scale bars: A-D, 5 μm.
FIG. 1 in Intraindividual variation in nuclear DNA content in Durvillaea antarctica (Chamisso) Hariot, Macrocystis pyrifera (Linnaeus) C. Agardh and Lessonia spicata (Suhr) Santelices (Phaeophyceae)
FIG. 1. — Cells of Durvillaea antarctica (Chamisso) Hariot stained with DAPI: A, mitotic figure (mf) of dividing cortical cells (cc); B, uninucleate cortical cells; C, D, mature antheridia (ma), antheridia germinative cells (agc), antheridium (a) and four-nucleate antheridium (4-na); E, five-nucleate antheridium (5-na); F, antheridia germinative cells. Scale bars: A-D, 5 μm.
Figure 3. Example relative fluorescence histograms for samples stained with propidium iodide. The 2C in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 3. Example relative fluorescence histograms for samples stained with propidium iodide. The 2C peaks represent diploid cells, and 4C peaks represent cells in the G2 phase of the cell cycle, with replicated DNA. Standard used: Solanum pseudocapsicum 2C = 2.61 pg. A, Himacerus apterus female with 2n = 36 + XX and 2C = 9.71 pg. B, Nabis maoricus female with 2n = 16 + XX and 2C = 4.21 pg.
Figure 2 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 2. Nuclear DNA content stained with propidium iodide (PI) compared with relative nuclear DNA content stained with 4′,6-diamidino-2-phenylindole (DAPI). The line represents the trend in GC content, with a minimum of 33.34% and a maximum of 37.83%. Each pair of black and white symbols represents one specimen, as follows: circles, females; squares, males; white symbols, DAPI; black symbols, PI.
Figure 1 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 1. Chromosomes of Nabidae species studied, stained with Giemsa (A, D, F, G, M) or with an 18S ribosomal DNA (rDNA) probe (red) applied via fluorescence in situ hybrization (FISH) (B, C, E, H–L, N–P). A, B, Nabis punctatus ♀ 2n = 16 + XX, mitotic metaphase. C, Himacerus apterus ♀ 2n = 36 + XX, mitotic metaphase. D, Nabis rugosus ♂ 2n = 16 + XY + 1 metaphase I, specimen with an additional chromosome (arrow). E, Nabis maoricus ♀ 2n = 18 + XX, mitotic metaphase. F, N. maoricus ♂ 2n = 16 + XY, postpachytene, with sex chromosomes superspiralized. G, H, Nabis biformis ♀ 2n = 16 + XX, mitotic metaphase, with two 18S rDNA signals on each X chromosome. I, Nabis limbatus ♀ 2n = 16 + XX, mitotic metaphase, species with the most distal 18S rDNA signal. J, N. rugosus ♂ 2n = 16 + XY, mitotic metaphase, species with the two 18S rDNA signals on Y chromosome. K, Prostemma guttula ♂ 2n = 26 + XY, metaphase II. L, N. maoricus ♂ 2n = 16 + XY, metaphase II, with Y chromosome showing no 18S rDNA signal. M, N, Prostemma aeneicolle ♀ 2n = 26 + XX, mitotic metaphase. O, H. apterus ♀ 2n = 36 + XXXX, mitotic metaphase, with terminal 18S rDNA signals on four X chromosomes originated by fragmentation. P, N. maoricus ♀ 2n = 18 + XXX, mitotic metaphase, with one X chromosome fragmented outside of the 18S rDNA position. Arrowheads indicate 18S rDNA signal; X and Y are the sex chromosomes. Scale bars: 10 μm.
Figure 4 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 4. Comparison of three alternative hypotheses on the ancestral 2n number of chromosomes of Nabidae: fusions, proposed by Nokkala et al. (2007); autosomal polyploidy, suggested by Kuznetsova & Maryańska-Nadachowska (2000) and supported by nuclear DNA content data from the present study; and the hypothetical fission theory. Abbreviations: 2C, nuclear DNA content; F, autosomal fusions; Fis, fissions; P, polyploidy.
FIGURE 4 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 4. Leaf cross sections: 1) Sternbergia candida, 2) S. clusiana, 3) S. colchiciflora, 4) S. lutea, 5) S. sicula, 6) S. vernalis.
FIGURE 1 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 1. Distribution of the genus Sternbergia in Turkey: S.candida, S.clusianȧ, S. colchiciflora, S.lutea, S. sicula, S. vernalis, S.schubertii?.
FIGURE 3 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 3. Mitotic chromosomes and idiograms of Sternbergia; 4) S. lutea, 5) S. sicula, 6) S. vernalis, (Scale bar = 10μm).
FIGURE 6 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 6. UPGMA cluster of the considered taxa, based on the morpho-anatomical data matrix presented in Table 3.
FIGURE 2 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 2. Mitotic chromosomes and idiograms of Sternbergia; 1) S. candida, 2) S. clusiana, 3) S. colchiciflora (Scale bar = 10μm).
FIGURE 5 in Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content
FIGURE 5. Leaf cross sections: 1) Sternbergia candida, 2) S. clusiana, 3) S. colchiciflora, 4) S. lutea, 5) S. sicula, 6) S. vernalis. (e: epidermis, s: stomata, pp: palisade parenchyma, sp: spongy parenchyma, ls: laticifer systems) (scale bar 0.1 mm).
Figure 4 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 4. Comparison of three alternative hypotheses on the ancestral 2n number of chromosomes of Nabidae: fusions, proposed by Nokkala et al. (2007); autosomal polyploidy, suggested by Kuznetsova & Maryańska-Nadachowska (2000) and supported by nuclear DNA content data from the present study; and the hypothetical fission theory. Abbreviations: 2C, nuclear DNA content; F, autosomal fusions; Fis, fissions; P, polyploidy.
Figure 3. Example relative fluorescence histograms for samples stained with propidium iodide. The 2C in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 3. Example relative fluorescence histograms for samples stained with propidium iodide. The 2C peaks represent diploid cells, and 4C peaks represent cells in the G2 phase of the cell cycle, with replicated DNA. Standard used: Solanum pseudocapsicum 2C = 2.61 pg. A, Himacerus apterus female with 2n = 36 + XX and 2C = 9.71 pg. B, Nabis maoricus female with 2n = 16 + XX and 2C = 4.21 pg.
FIG. 2 in Intraindividual variation in nuclear DNA content in Durvillaea antarctica (Chamisso) Hariot, Macrocystis pyrifera (Linnaeus) C. Agardh and Lessonia spicata (Suhr) Santelices (Phaeophyceae)
FIG. 2. — Developmental stages of sporangia in Lessonia spicata (Suhr) Santelices stained with DAPI: A, sporangia (s) and sporangial mother cells (smc); B, four-nucleate sporangium (4-ns) and sporangial mother cells (smc); C, D, different developmental stages of the sporangia. Scale bars A-D, 5 μm.
Figure 2 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 2. Nuclear DNA content stained with propidium iodide (PI) compared with relative nuclear DNA content stained with 4′,6-diamidino-2-phenylindole (DAPI). The line represents the trend in GC content, with a minimum of 33.34% and a maximum of 37.83%. Each pair of black and white symbols represents one specimen, as follows: circles, females; squares, males; white symbols, DAPI; black symbols, PI.
Figure 1 in Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling
Figure 1. Chromosomes of Nabidae species studied, stained with Giemsa (A, D, F, G, M) or with an 18S ribosomal DNA (rDNA) probe (red) applied via fluorescence in situ hybrization (FISH) (B, C, E, H–L, N–P). A, B, Nabis punctatus ♀ 2n = 16 + XX, mitotic metaphase. C, Himacerus apterus ♀ 2n = 36 + XX, mitotic metaphase. D, Nabis rugosus ♂ 2n = 16 + XY + 1 metaphase I, specimen with an additional chromosome (arrow). E, Nabis maoricus ♀ 2n = 18 + XX, mitotic metaphase. F, N. maoricus ♂ 2n = 16 + XY, postpachytene, with sex chromosomes superspiralized. G, H, Nabis biformis ♀ 2n = 16 + XX, mitotic metaphase, with two 18S rDNA signals on each X chromosome. I, Nabis limbatus ♀ 2n = 16 + XX, mitotic metaphase, species with the most distal 18S rDNA signal. J, N. rugosus ♂ 2n = 16 + XY, mitotic metaphase, species with the two 18S rDNA signals on Y chromosome. K, Prostemma guttula ♂ 2n = 26 + XY, metaphase II. L, N. maoricus ♂ 2n = 16 + XY, metaphase II, with Y chromosome showing no 18S rDNA signal. M, N, Prostemma aeneicolle ♀ 2n = 26 + XX, mitotic metaphase. O, H. apterus ♀ 2n = 36 + XXXX, mitotic metaphase, with terminal 18S rDNA signals on four X chromosomes originated by fragmentation. P, N. maoricus ♀ 2n = 18 + XXX, mitotic metaphase, with one X chromosome fragmented outside of the 18S rDNA position. Arrowheads indicate 18S rDNA signal; X and Y are the sex chromosomes. Scale bars: 10 μm.
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