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10 results for “oblique plane microscopy”
Raw data from the manuscript "Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing"
<p>The repository contains all the raw data from the manuscript titled "Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing" (https://doi.org/10.1117/1.JBO.29.3.036502).<br> The data consists in 3D stacks acquired with the method described in the manuscript. Since raw images are acquired along a diagonal plane, and are stretched in one direction, the dataset also includes a Python script to perform an affine transform projecting the stack on cartesian coordinates.</p> <p>Samples imaged include sub-resolution microbeads in agarose gel, a fixed slice of mouse kidney (fluocells prepared slide #3, invitrogen), and 3 to 5 days post fertilization Tg(kdrl:eGFP)s843 Zebrafish.</p>
Text-fig. 1. Gastonispermum portugallicum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). Note remains of mounting media on several seeds (¤). a) Seed in oblique view showing seed shape, the slightly raised raphal ridge and the position of hilum (hi) and micropyle (mi) on the raphal side of the seed (S170218). b, c) Seeds in lateral view (b, S170234; c, S175095). d–f) Holotype (S174820); seed in lateral view (d) and cut volume rendering (e, f) through the median plane of the seed showing palisade-shaped sclerenchyma cells of exotesta and remains of embryo (emb) and surrounding nutritive tissue (e, cut between yz0440-0530; f, cut between slices yz440-480). g) Hilum (hi) and micropyle (mi) of seed in (1a) showing the Y-shaped micropylar slit in the outer integument. h) Cut volume rendering through the median plane of the seed (cut at yz0492) showing seed coat mainly composed of palisade-shaped cells of the exotesta (S174435). i) Seed surface showing the raised outlines of the undulate anticlinal walls of the exotestal cells (S175045). Scale bars = 500 µm (a–e); 250 µm (g); 125 µm (f, i). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 1. Gastonispermum portugallicum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). Note remains of mounting media on several seeds (¤). a) Seed in oblique view showing seed shape, the slightly raised raphal ridge and the position of hilum (hi) and micropyle (mi) on the raphal side of the seed (S170218). b, c) Seeds in lateral view (b, S170234; c, S175095). d–f) Holotype (S174820); seed in lateral view (d) and cut volume rendering (e, f) through the median plane of the seed showing palisade-shaped sclerenchyma cells of exotesta and remains of embryo (emb) and surrounding nutritive tissue (e, cut between yz0440-0530; f, cut between slices yz440-480). g) Hilum (hi) and micropyle (mi) of seed in (1a) showing the Y-shaped micropylar slit in the outer integument. h) Cut volume rendering through the median plane of the seed (cut at yz0492) showing seed coat mainly composed of palisade-shaped cells of the exotesta (S174435). i) Seed surface showing the raised outlines of the undulate anticlinal walls of the exotestal cells (S175045). Scale bars = 500 µm (a–e); 250 µm (g); 125 µm (f, i).
Text-fig. 11. Silutanispermum kvacekiorum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a, b) Holotype (S170238), seed in oblique (a) and lateral (b) view showing large triangular hilar scar (hi) and transverse micropylar slit (mi). c) Oblique view of seed showing slightly raised raphal area (S174352); remains of mounting media (¤). d) Details of holotype showing triangular hilum (hi) and transverse micropylar slit in the exotesta (mi). e) Cut volume rendering of holotype (cut at yz1170) through the median plane showing hilum (hi) and micropylar slit (mi) lined by radially expanded exotestal cells. Scale bars = 500 µm (a–c); 250 µm (d, e). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 11. Silutanispermum kvacekiorum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a, b) Holotype (S170238), seed in oblique (a) and lateral (b) view showing large triangular hilar scar (hi) and transverse micropylar slit (mi). c) Oblique view of seed showing slightly raised raphal area (S174352); remains of mounting media (¤). d) Details of holotype showing triangular hilum (hi) and transverse micropylar slit in the exotesta (mi). e) Cut volume rendering of holotype (cut at yz1170) through the median plane showing hilum (hi) and micropylar slit (mi) lined by radially expanded exotestal cells. Scale bars = 500 µm (a–c); 250 µm (d, e).
Hyperspectral Oblique Plane Microscopy - microparticles
<p>Processed spectra data for microparticle classification and raw hyperspectral images from mixture of microparticles</p>
Mouse Organoids imaged with Dual Oblique Plane Microscopy
<p>This is a dataset to demonstrate segmenting colonies of 3D cells. The primary focus is segmenting mouse organoids images using timelapse dual oblique plane microscopy. There is also a set of data mouse embryonic stem cells imaged using spinning disk confocal microscopy.</p>
Dual-view oblique plane microscopy (dOPM)
<p>Dual-view oblique plane microscopy (dOPM), View 1 and View 2 tiff stack volumes for:</p> <ul> <li>200 nm TetraSpeck beads embedded in 1% agarose</li> <li>100 nm TetraSpeck beads embedded in 1% agarose</li> <li>A fixed spheroid of WMs cells embedded in Matrigel and where Alexa Fluor™ 488 Phalloidin fluorescently labels actin</li> </ul> <p>The data is organised according to the figures they are related to in the following publication:</p> <p>Sparks. H, Dent. L,Bakal. C, Behrens. A, Salbreux. G, Dunsby. C, "Dual-view oblique plane microscopy (dOPM)," <a href="https://doi.org/10.1364/BOE.409781">https://doi.org/10.1364/BOE.409781</a> (2020)</p>
Hyperspectral Oblique Plane Microscopy -- microparticles 3D & laser beam profile & hyperspectral image of UV adhesive
<p>Processed spectra data for microparticle classification and raw hyperspectral images from mixture of microparticle</p>
Hyperspectral Oblique Plane Microscopy -- CAD model and PSF
<p>CAD model (without Raman laser) and PSF data</p>
Hyperspectral Oblique Plane Microscopy -- zebrafish wound
<p>Data and postprocessing codes for the zebrafish embryo wound experiment. </p>
Hyperspectral Oblique Plane Microscopy -- heart
<p>Data and postprocessing codes for the zebrafish embryo heart experiment</p>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.