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Dataset results
301 results for “over-expression”
Transcriptome analysis of the effect of over-expressing H2A.J mutants in proliferating WI38 fibroblasts for the paper entitled: The H2A.J histone variant contributes to Interferon-Stimulated Gene expression in senescence by its weak interaction with H1 and the derepression of repeated DNA sequences
<p>Abstract for overall study:</p> <p>The histone variant H2A.J was previously shown to accumulate in senescent human fibroblasts with persistent DNA damage to promote inflammatory gene expression, but its mechanism of action was unknown. We show that H2A.J accumulation contributes to weakening the association of histone H1 to chromatin and increasing its turnover. Decreased H1 in senescence is correlated with increased expression of some repeated DNA sequences, increased expression of STAT/IRF transcription factors, and transcriptional activation of Interferon-Stimulated Genes (ISGs). The H2A.J-specific Val-11 moderates the transcriptional activity of H2A.J, and H2A.J-specific Ser-123 can be phosphorylated in response to DNA damage with potentiation of its transcriptional activity by the phospho-mimetic S123E mutation. Our work demonstrates the functional importance of H2A.J-specific residues and potential mechanisms for its function in promoting inflammatory gene expression in senescence.</p> <p>Specific description for this dataset:</p> <p>H2A.J differs from canonical H2A only by a valine at position 11 instead of alanine, and the 7 C-terminal amino acids containing a potential minimal phosphorylation site SQ for DNA-damage response kinases. To test the functional importance of these H2A.J-specific sequences, we mutated Val-11 to Ala as is found in all canonical H2A sequences, and we mutated Ser-123 to either Glu to mimic a phospho-serine residue or to Ala to prevent phosphorylation. We also substituted the C-terminus of H2A.J with the C-terminus of H2A. These mutants, WT-H2A.J and canonical H2A-type1 were ectopically expressed in proliferating fibroblasts, and their microarray transcriptomes were compared to that of proliferating and senescent fibroblasts without ectopic histone expression. Genome-wide transcriptome analysis indicated that senescent fibroblasts clustered distinctly from proliferating fibroblasts, and proliferating fibroblasts expressing the H2A.J-V11A and H2A.J-S123E mutants clustered distinctly from fibroblasts expressing the other H2A.J mutants, WT-H2A.J, and H2A. Hallmark gene set enrichment analysis of the transcriptomes of fibroblasts expressing H2A.J-V11A or H2A.J-S123E versus control proliferating fibroblasts indicated that they showed the same highly significant enrichment for the Epithelial-Mesenchyme Transition, TNF-Alpha Signaling Via NF-kB, and Inflammatory Response gene sets. Notable inflammatory genes including IL1A, IL1B, IL6, CXCL8, and CCL2 are contained in these gene sets and are often induced in senescence as part of the senescence-associated secretory phenotype. Heat maps showed that the H2A.J-V11A and H2A.J-S123E mutants were particularly apt at activating the expression of these inflammatory genes in proliferating fibroblasts</p>
Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02
<p>Structure-function open lab notebook project. Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02.</p>
ErbB2 Over-expressing Metastatic Breast Cancer Study Using Paclitaxel, Trastuzumab, and Lapatinib
ClinicalTrials.gov study NCT00272987. IPD Sharing: YES. Countries: 2. Publications: 1.
Vinflunine Plus Trastuzumab in Human Epidermal Growth Factor Receptor 2 (HER2neu) Over-Expressing Metastatic Breast Cancer
ClinicalTrials.gov study NCT00284180. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Breast Cancer With Over-expression of erbB2-BRAINSTORM
ClinicalTrials.gov study NCT01064349. IPD Sharing: Not stated. Countries: 3. Publications: 1.
GW572016 With Docetaxel and Trastuzumab for the Treatment Of Untreated ErbB2 Over-Expressing Metastatic Breast Cancer
ClinicalTrials.gov study NCT00251433. IPD Sharing: NO. Countries: 3. Publications: 1.
Over-expression of Ki-67 as a predictor of lymph node metastasis in penile cancer patients
<p>In this study, we will look at the association of Ki-67 with lymph node metastasis in patients with penile cancer.</p>
Study of Glembatumumab Vedotin (CDX-011) in Patients With Metastatic, gpNMB Over-Expressing, Triple Negative Breast Cancer
ClinicalTrials.gov study NCT01997333. IPD Sharing: Not stated. Countries: 9. Publications: 0.
Dovitinib in BCG Refractory Urothelial Carcinoma With FGFR3 Mutations or Over-expression
ClinicalTrials.gov study NCT01732107. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Analysis of Brain Metastasis in Patients With Breast Cancer, With and Without Over-Expression of HER-2
ClinicalTrials.gov study NCT00071383. IPD Sharing: Not stated. Countries: 1. Publications: 3.
A Study of IMU-131 (HER-Vaxx) in Combination With Chemotherapy or Pembrolizumab in Patients With Metastatic HER2/Neu Over-Expressing Gastric Cancer (nextHERIZON)
ClinicalTrials.gov study NCT05311176. IPD Sharing: NO. Countries: 2. Publications: 0.
Gene-expression analysis of Oncostatin-M (OSM) signalling in cervical squamous cell carcinomas over-expressing the Oncostatin-M receptor (OSMR)
GEO Series GSE27480. Homo sapiens. 72 samples. Type: Expression profiling by array.
Transcriptomic analysis of neuroblastoma cells in response to stable over-expression of Ets-1 promoter-associated noncoding RNA (pancEts-1)
GEO Series GSE104950. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Gene expression analysis of miR-210 over-expressing flies at ZT12.
GEO Series GSE77233. Drosophila melanogaster. 8 samples. Type: Expression profiling by array.
Primary hepatocyte transcriptional response to Fis1 and Drp1 over-expression by adenovirus
GEO Series GSE169630. Mus musculus. 16 samples. Type: Expression profiling by high throughput sequencing.
The global gene expression pattern by KDX1 over-expression
GEO Series GSE45692. Saccharomyces cerevisiae. 1 samples. Type: Expression profiling by array.
RNA-Seq of SLNCR1 over-expression in the melanoma cell line A375
GEO Series GSE77901. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
The effect of over-expression of PRR5-VP in Arabidopsis seedlings
GEO Series GSE36360. Arabidopsis thaliana. 20 samples. Type: Expression profiling by array.
RNA-Seq data for AKT, BAD, ERBB2, IGF1R, RAF1 and ERK1 over-expressed samples with twelve green fluorescent protein control samples using human mammary epithelial cells
GEO Series GSE73628. Homo sapiens. 47 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide maps of WT and over-expressing CenH3/CENP-A in Human HeLa S3 cells
GEO Series GSE42951. Homo sapiens. 16 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.