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20 results for “pH adaptation”
Data from: Plant ammonium sensitivity is associated with the external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement
<p>Modern crops exhibit diverse sensitivities to ammonium as the primary nitrogen source, influenced by environmental factors such as external pH and nutrient availability. Despite its significance, there is currently no systematic classification of plant species based on their ammonium sensitivity. This study conducts a meta-analysis of 50 plant species and presents a new classification method based on the comparison of fresh biomass obtained under ammonium and nitrate nutrition. The classification uses the natural logarithm of biomass ratio as the size effect indicator of ammonium sensitivity. This numerical parameter is associated with critical factors for nitrogen demand and form preference, such as Ellenberg indicators and the repertoire of nitrogen transporters for ammonium and nitrate uptake. Finally, a comparative analysis of the developmental and metabolic responses, including hormonal balance, is conducted in two species with divergent ammonium sensitivity values in the classification. Results indicate that nitrate has a key counteracting role of ammonium toxicity in species with a higher abundance of genes encoding NRT2-type proteins and fewer of the AMT2-type proteins. Additionally, the study confirms the reliability of the phytohormone balance and methylglyoxal content as indicators for anticipating ammonium toxicity.</p>
Data from: Plant ammonium sensitivity is associated with the external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement
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Corals adapted to extreme and fluctuating seawater pH increase calcification rates and have unique symbiont communities
<p>Ocean acidification (OA) is a severe threat to coral reefs mainly by reducing their calcification rate. Identifying the resilience factors of corals to decreasing seawater pH is of paramount importance to predict the survivability of coral reefs in the future. This study compared corals adapted to variable pH<sub>T</sub> (i.e., 7.23–8.06) from the semi-enclosed lagoon of Bouraké, New Caledonia, to corals adapted to more stable seawater pH<sub>T</sub> (i.e., 7.90-8.18). In a 100-day aquarium experiment, we examined the physiological response and genetic diversity of Symbiodiniaceae from three coral species (<em>Acropora tenuis</em>, <em>Montipora</em> <em>digitata</em> and <em>Porites</em> sp.) from both sites under three stable pH<sub>NBS</sub> conditions (8.11, 7.76, 7.54) and one fluctuating pHNBS regime (between 7.56 and 8.07). Bouraké corals consistently exhibited higher growth rates than corals from the stable pH environment. Interestingly, <em>A</em>. <em>tenuis</em> from Bouraké showed the highest growth rate under the 7.76 pH<sub>NBS</sub> condition, whereas for <em>M. digitata</em> and <em>Porites</em> sp. from Bouraké, growth was highest under the fluctuating regime and the 8.11 pH<sub>NBS</sub> conditions, respectively. While OA generally decreased coral calcification by ca. 16%, Bouraké corals showed higher growth rates than corals from the stable pH environment (21% increase for <em>A. tenuis</em> to 93% for <em>M. digitata</em>, with all pH conditions pooled). This superior performance coincided with divergent symbiont communities that were more homogenous for Bouraké corals. Corals adapted to variable pH conditions appear to have a better capacity to calcify under reduced pH compared to corals native to more stable pH conditions. This response was not gained by corals from the more stable environment exposed to variable pH during the 100-day experiment, suggesting that long-term exposure to pH fluctuations and/or differences in symbiont communities benefit calcification under OA.</p>
Molecular in situ monitoring of the pH-triggered response in adaptive polymers by two-dimensional Raman micro-correlation-spectroscopy - Raw Data
<p>Raw data for publication Molecular in situ monitoring of the pH-triggered response in adaptive polymers by two-dimensional Raman micro-correlation-spectroscopy</p>
A Phase 3 Adaptive Study to Evaluate the Safety and Efficacy of Inhaled Treprostinil in Participants With Pulmonary Hypertension (PH) Due to Chronic Obstructive Pulmonary Disease (COPD)
ClinicalTrials.gov study NCT03496623. IPD Sharing: NO. Countries: 4. Publications: 1.
Corals adapted to extreme and fluctuating seawater pH increase calcification rates and have unique symbiont communities
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Fig. 6 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 6. Longevity of AmSOD1 activity. Purified His-AmSOD1 was tested for its longevity both at room temperature and 4 ◦ C for different time period (20, 40, 60, 80, 100, 120 1st 140 days). The ratio enzyme activity was determined based on the activity of enzyme before incubation. Each data point represents the mean ± SD obtained from three independent reactions.
Fig. 7 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 7. Growth curves of the strains control and R-AmSOD1 in the medium without treatment and the medium containing containing (A) salt and (B) hydrogen peroxide. Each data point represents the mean ± SD obtained from three independent reactions.
Fig. 2 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 2. The effects of salt and hydrogen peroxide stress on the appearance pattern and activity of AmSOD1 in the leaves of A. marina (A) Appearance pattern of AmSOD1 and (B) AmSOD assay in the leaves responding to hydrogen peroxide and salt stress as well as control at various time points (0–48 h). Each histogram represents the mean obtained from three independent reactions (± SD). Different letters above the bars indicate statistically significant differences by LSD test.
Fig. 1 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 1. Phylogenetic tree of SOD sequences from different plants. For accession numbers and organisms, see ''Materials and Methods.''.
Fig. 4 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 4. Molecular mass of purified AmSOD1 using gel filtration. (A) Native molecular mass of recombinant AmSOD1 was measured with respect to standard protein marker as resolved in Biosil-250 analytical gel filtration column. Standard proteins used were: (a) 66.5 kDa-BSA fraction (b) 29 kDa-Carbonic anhydrase and (c) 16.7 kDa human myoglubin.
Fig. 3 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 3. SDS-PAGE analysis and the solubility of expressed His-AmSOD1 in the strain R-AmSOD1 harboring pET28a-AmSOD1 (A) Total soluble protein extracted from control strain and the strain R-AmSOD1 before and 1–4 h after induction with IPTG. (B) The study of solubility of His-AmSOD1 by comparison of His-AmSOD1 band intensities in the total protein (T), the soluble fraction extracted from R-AmSOD1 (S) and the unsoluble fraction (U) extracted from RAmSOD1 (The recombinant proteins were shown with arrows) (C) SDS-PAGE analysis of AmSOD1 after purification with affinity chromatography.
Fig. 5 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Fig. 5. Effects of incubation at different temperatures and pH on the activity of AmSOD1. The purified AmSOD1 was incubated (A) at temperatures (25, 37, 50, 60 and 75 ◦ C) (B) in buffers with pH (3–12) for 1 h before SOD assay. Then the activity of AmSOD1 was determined as explained in the "Materials and Methods". Each data point represents the mean ± SD obtained from three independent reactions.
"Geriatric Assessment Adapted" Therapy for Ph- ALL Elderly Patients
ClinicalTrials.gov study NCT00475280. IPD Sharing: NO. Countries: 1. Publications: 10.
Data from: Coupling of diversification and pH adaptation during the evolution of terrestrial Thaumarchaeota
The Thaumarchaeota is an abundant and ubiquitous phylum of archaea that plays a major role in the global nitrogen cycle. Previous analyses of the ammonia monooxygenase gene amoA suggest that pH is an important driver of niche specialization in these organisms. Although the ecological distribution and ecophysiology of extant Thaumarchaeota have been studied extensively, the evolutionary rise of these prokaryotes to ecological dominance in many habitats remains poorly understood. To characterize processes leading to their diversification, we investigated coevolutionary relationships between amoA, a conserved marker gene for Thaumarchaeota, and soil characteristics, by using deep sequencing and comprehensive environmental data in Bayesian comparative phylogenetics. These analyses reveal a large and rapid increase in diversification rates during early thaumarchaeotal evolution; this finding was verified by independent analyses of 16S rRNA. Our findings suggest that the entire Thaumarchaeota diversification regime was strikingly coupled to pH adaptation but less clearly correlated with several other tested environmental factors. Interestingly, the early radiation event coincided with a period of pH adaptation that enabled the terrestrial Thaumarchaeota ancestor to initially move from neutral to more acidic and alkaline conditions. In contrast to classic evolutionary models, whereby niches become rapidly filled after adaptive radiation, global diversification rates have remained stably high in Thaumarchaeota during the past 400–700 million years, suggesting an ongoing high rate of niche formation or switching for these microbes. Our study highlights the enduring importance of environmental adaptation during thaumarchaeotal evolution and, to our knowledge, is the first to link evolutionary diversification to environmental adaptation in a prokaryotic phylum.
Data from: Coupling of diversification and pH adaptation during the evolution of terrestrial Thaumarchaeota
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Effects of dietary forage and calf starter on ruminal pH and transcriptomic adaptation of the rumen epithelium in Holstein calves during the weaning transition
GEO Series GSE83813. Bos taurus. 7 samples. Type: Expression profiling by array.
Lactobacillus plantarum IMDO 130201, a wheat sourdough isolate, adapts to growth in wheat sourdough simulation medium at different pH values through differential gene expression
GEO Series GSE23945. Leuconostoc mesenteroides; Pediococcus acidilactici; Pediococcus pentosaceus; Enterococcus faecium; Levilactobacillus brevis; Lactobacillus helveticus; Lactiplantibacillus plantarum; Companilactobacillus alimentarius; Fructilactobacillus fructivorans; Leuconostoc citreum; Limosilactobacillus pontis; Lactobacillus crispatus; Lactiplantibacillus paraplantarum; Limosilactobacillus frumenti; Furfurilactobacillus rossiae; Staphylococcus aureus; Enterococcus faecalis; Enterococcus hirae; Lactococcus lactis; Lacticaseibacillus casei; Lacticaseibacillus paracasei; Companilactobacillus farciminis; Limosilactobacillus fermentum; Fructilactobacillus sanfranciscensis; Bifidobacterium; Lacticaseibacillus rhamnosus; Enterococcus mundtii; Lentilactobacillus parabuchneri; Lentilactobacillus buchneri; Lactobacillus delbrueckii; Lactiplantibacillus pentosus; Lactobacillus gasseri; Limosilactobacillus reuteri; Lactobacillus amylovorus; Latilactobacillus curvatus; Lactobacillus johnso
NRG1 as a regulator of pH adaptation in the Basidiomycota Ustilago maydis
GEO Series GSE133840. Mycosarcoma maydis. 8 samples. Type: Expression profiling by high throughput sequencing.
Effects of nitric oxide on Salmonella adapted at pH 4.4
GEO Series GSE9398. Salmonella; Salmonella enterica subsp. enterica serovar Typhimurium. 3 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.