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123 results for “phagocytosis”
Systemic Treatment with Cigarette Smoke Extract Affects Zebrafish Visual Behaviour, Intraocular Vasculature Morphology and Outer Segment Phagocytosis
<p>Underlying dataset and analysis tests of the results described in the article "Systemic Treatment with Cigarette Smoke Extract Affects Zebrafish Visual Behaviour, Intraocular Vasculature Morphology and Outer Segment Phagocytosis".</p>
Macrophage phagocytosis assay of Acinetobacter baumannii AB074, passaged 15 days in antibiotics or transferrin.
<p>Macrophage phagocytosis assay of Acinetobacter baumannii AB074, passaged 15 days in antibiotics or transferrin. Experiment #1, replica #1-2, from March 7th, 2017 and Experiment #2, replica #1-2 from March 16th, 2017</p> <p>MICs for AB074: transferrin = 4 mcg/ml; ciprofloxacin = 1 mcg/ml; meropenem = 0,5 mcg/ml</p> <p>6 groups of treatment:</p> <p>1. No drug</p> <p>2. Transferrin only, last passage dose = 16 mcg/ml </p> <p>3. Ciprofloxacin only, last passage dose = 5 mcg/ml </p> <p>4. Ciprofloxacin + transferrin, last passage dose = 0,5 mcg/ml of cipro + 16 mcg/ml of transferrin</p> <p>5. Meropenem only, last passage dose = 2,5 mcg/ml </p> <p>6. Meropenem + transferrin, last passage dose = 2,5 mcg/ml + 16 mcg/ml of transferrin</p> <p>The file name structure: group_picture#_strain_timeOfPassage_ experiment#_replica#</p>
Fig 2 in Docosahexaenoic Acid (DHA) Reduces LPSInduced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/ Syk Signaling-Dependent Phagocytosis in Microglia
<p>Proteome profiler arrays (A and B) and expression of ATF3 gene (C) in microglia. Representative array membranes (A) and the relative levels of cytokines and chemokines (B) in microglia preincubated with 20 μM DHA and next treated with 10 ng/ml LPS.</p>
Fig 1 in Docosahexaenoic Acid (DHA) Reduces LPSInduced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/ Syk Signaling-Dependent Phagocytosis in Microglia
<p>Viability of microglia incubated with various concentration of DHA for 12 h (A) and LPS for 2.5 h (B). Viability of microglia incubated with 20 μM DHA followed by 10 ng/ ml LPS treatment (C).</p>
Phagocytosis of E. coli bacteria by macrophages
<p>Holotomography imaging of RAW 264.7 murine macrophages phagocytosing E. coli bacteria; performed in the Meijer laboratory at the Institute of Biology Leiden, Leiden University.</p> <p> </p> <p> </p>
Macrophage Phagocytosis in COPD
ClinicalTrials.gov study NCT00298389. IPD Sharing: NO. Countries: 1. Publications: 1.
Myeloid PTEN loss affects the therapeutic response by promoting stress granule assembly and impairing phagocytosis by macrophages in breast cancer
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REV-ERBalpha mediates complement expression and circadian regulation of microglial synaptic phagocytosis
<p>The circadian clock has been shown to regulate various aspects of brain health including microglial and astrocyte activation. Here we report that deletion of the master clock protein BMAL1 induces robust increases in the expression of complement genes such as <i>C3, C4b </i>and <i>C1q</i> in the hippocampus. Loss of downstream REV-ERBa-mediated transcriptional repression led to increases in <i>C4b </i>in neurons and astrocytes as well as C3 protein in microglia and astrocytes. REV-ERBa deletion induced complement <i>C3/C4b </i>gene expression and increased microglial phagocytosis of synapses in the CA3 region of the hippocampus. Finally, we observed diurnal variation in the degree of microglial synaptic phagocytosis in wild type mice which was abrogated by REV-ERBα deletion. This work uncovers the BMAL1-REV-ERBa axis as a regulator of complement expression and synaptic phagocytosis in the brain, thereby illuminating a novel mechanism of synaptic regulation by the circadian clock.</p>
Data from: Reduced internalization of TNF-ɑ/TNFR1 down-regulates caspase dependent phagocytosis induced cell death (PICD) in neonatal monocytes
Phagocytosis-induced cell death (PICD) is diminished in cord blood monocytes (CBMO) as compared to cells from adults (PBMO) due to differences in the CD95-pathway. This may support a prolonged pro-inflammatory response with sequels of sustained inflammation as seen in neonatal sepsis. Here we hypothesized that TNF-α mediated induction of apoptosis is impaired in CBMO due to differences in the TNFR1-dependent internalization. Monocytes were infected with Escherichia coli-GFP (E. coli-GFP). Monocyte phenotype, phagocytic activity, induction of apoptosis, and TNF-α/TNF-receptor (TNFR) -expression were analysed. In the course of infection TNF-α-secretion of CBMO was reduced to 40% as compared to PBMO (p<0.05). Neutralization of TNF-α by an αTNF-α antibody reduced apoptotic PICD in PBMO four-fold (p < 0.05 vs. infection with E. coli). PICD in CBMO was reduced 5-fold compared to PBMO and showed less responsiveness to αTNF-α antibody. CBMO expressed less pro-apoptotic TNFR1, which, after administration of TNF-α or infection with E. coli was internalized to a lesser extent. With similar phagocytic capacity, reduced TNFR1 internalization in CBMO was accompanied by lower activation of caspase-8 (p < 0.05 vs. PBMO). Stronger caspase-8 activation in PBMO caused more activation of effector caspase-3 and apoptosis (all p < 0.05 vs. PBMO). Our results demonstrate that TNFR1 internalization is critical in mediating PICD in monocytes after infection with E.coli and is reduced in CBMO.
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium
<p>This dataset contains all patch clamp, confocal and mass spectrometry datasets of our publication 'Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium', published in BMC Biology. The data for each figure (fig 1-7) and supplementary figure (1-3) has been collected to an individual zipped folder. For additional information, please contact the corresponding author (Soile Nymark).</p>
Flow Cytometry Data files for Settle et al. Nature Communications, B2 integrins impose a mechanical checkpoint on macrophage phagocytosis
<p>FCS Data files for all flow cytometry results for Settle et al, B2 integrins impose a mechanical checkpoint on macrophage phagocytosis, to be published in Nature Communications. </p>
Fig 5 in Docosahexaenoic Acid (DHA) Reduces LPS-Induced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/Syk Signaling-Dependent Phagocytosis in Microglia
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Fig 3 in Docosahexaenoic Acid (DHA) Reduces LPSInduced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/ Syk Signaling-Dependent Phagocytosis in Microglia
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Fig 4 in Docosahexaenoic Acid (DHA) Reduces LPS-Induced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/Syk Signaling-Dependent Phagocytosis in Microglia
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Data for: Microglia phagocytosis determines the volume and function of the rat Sexually Dimorphic Nucleus of the Preoptic Area
<p>The Sexually Dimorphic Nucleus of the Preoptic Area (SDN-POA) is the oldest and most robust sex difference reported in mammalian brain and is singular for its presence across a wide range of species from rodents to ungulates to man. This small collection of Nissl dense neurons is reliably larger in volume in males. Yet, despite its notoriety and intense interrogation, both the mechanism establishing the sex difference and the functional role of the SDN have remained elusive. Convergent evidence from rodent studies led to the conclusion that testicular androgens aromatized to estrogens are neuroprotective in males and that higher apoptosis (naturally occurring cell death) in females determines their smaller SDN. In several species, including humans, a smaller SDN correlates with a preference for mating with males. We report here that this volume difference is dependent upon a participatory role of phagocytic microglia which engulf more neurons in the female SDN and assure their destruction . Selectively blocking microglia phagocytosis temporarily spared neurons from apoptotic death and increased SDN volume in females without hormone treatment. Increasing the number of neurons in the SDN in neonatal females resulted in loss of preference for male odors in adulthood, an effect paralleled by dampened excitation of SDN neurons as evidenced by reduced IEG expression when exposed to male urine. Thus, the mechanism establishing a sex difference in SDN volume includes an essential role for microglia, and SDN function as a regulator of sexual partner preference is confirmed.</p>
Data from: Reduced internalization of TNF-ɑ/TNFR1 down-regulates caspase dependent phagocytosis induced cell death (PICD) in neonatal monocytes
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REV-ERBalpha mediates complement expression and circadian regulation of microglial synaptic phagocytosis
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Data for: Microglia phagocytosis determines the volume and function of the rat Sexually Dimorphic Nucleus of the Preoptic Area
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Data from: Chimeric antigen receptors that trigger phagocytosis
Chimeric antigen receptors (CARs) are synthetic receptors that reprogram T cells to kill cancer. The success of CAR-T cell therapies highlights the promise of programmed immunity and suggests that applying CAR strategies to other immune cell lineages may be beneficial. Here, we engineered a family of Chimeric Antigen Receptors for Phagocytosis (CAR-Ps) that direct macrophages to engulf specific targets, including cancer cells. CAR-Ps consist of an extracellular antibody fragment, which can be modified to direct CAR-P activity towards specific antigens. By screening a panel of engulfment receptor intracellular domains, we found that the cytosolic domains from Megf10 and FcRɣ robustly triggered engulfment independently of their native extracellular domain. We show that CAR-Ps drive specific engulfment of antigen-coated synthetic particles and whole human cancer cells. Addition of a tandem PI3K recruitment domain increased cancer cell engulfment. Finally, we show that CAR-P expressing murine macrophages reduce cancer cell number in co-culture by over 40%.
PhagoStat a scalable and interpretable end to end framework for efficient quantification of cell phagocytosis in neurodegenerative disease studies
<p>This study presents a comprehensive dataset that captures the phagocytosis process in wild-type and FTD microglial cells, with each acquisition comprising 20 distinct scenes and seven hours of time-lapse video microscopy. The dataset has undergone rigorous validation and data quality correction techniques, ensuring its reliability for further analysis. The dataset includes 36,496 normalized cell and aggregate images, 1,306,131 individual instance masks for cells, 1,553,036 for aggregates, and 36,496 registered aggregates, all generated using the PhagoStat algorithm. The dataset also provides extensive biological features such as area, position, and speed, presented frame-by-frame for in-depth analysis. To enhance its value, 226 manually annotated images containing 6,430 individual cell masks were incorporated, covering different conditions and scenes. In total, the dataset comprises 235,288 files (94GB), offering researchers a valuable resource for investigating various cellular and aggregate properties.</p> <p>By providing this comprehensive dataset, the study aims to facilitate the development and application of novel methodologies in bioinformatics, computational modeling, and cellular biology. This initiative promotes innovation and collaboration among researchers in these interconnected disciplines, ultimately contributing to the understanding of cellular dynamics and the development of novel therapeutic strategies for neurodegenerative diseases like FTD.</p> <p>For more infomration please check:</p> <p>GitHub: <a href="https://github.com/ounissimehdi/PhagoStat">https://github.com/ounissimehdi/PhagoStat</a></p> <p>Paper: <a href="https://www.nature.com/articles/s41598-024-56081-7">https://www.nature.com/articles/s41598-024-56081-7</a></p> <p>DOI: 10.1038/s41598-024-56081-7</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.