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7 results for “phospholipid fatty acids”
Microbiome, mixotrophic algae, zooplankton, and fish amino acid and phospholipid fatty acid content in terrestrial and plastic carbon treatments
<p>Data includes amino acid (µg AA mg DW<sup>-1</sup>) and phospholipid fatty acid content (µg FA mg DW<sup>-1</sup>) of the microbiome, mixotrophic algae, zooplankton, and fish<em> </em>from the four-trophic level experiment. The experiment included control (no addition), 13.5% <sup>13</sup>C-labelled beech leaves (<em>Fagus sylvatica</em>), 97% <sup>13</sup>C-labelled lignin-hemicellulose extracted from wheat (<em>Triticum aestivum</em>, ~80% lignin, 13% hemicellulose), and 99% <sup>13</sup>C-labelled polystyrene (microplastic). Incubation time in humic lake water was 14 days in the control, leaf, and lignin experiment but 56 days for polystyrene, which after mixotrophic algae (<em>Cryptomonas </em>sp.) was introduced to the bottles. In the next step, herbivorous zooplankton (<em>Daphnia magna</em>) consumed microbes, mixotrophic algae, and particles for five days which after they were used as the diet to zebrafish (<em>Danio rerio</em>) during a five-day experiment.</p>
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Peat Exposed to Experimentally Increased N Deposition, 2015
Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. Bogs, being ombrotrophic, may be especially susceptible to increasing N deposition. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a bog near Mariana Lakes, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha-1 yr-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. For the most part, microbial group abundances were not affected by increasing N input (Fig. 16). However, actinomycete abundance decreased with increasing N deposition at rates that were similar in 0-5 and 5-10 cm peat. Gram-negative bacteria increased slightly with increasing N input and were more abundant in 0-5 cm than in 5-10 cm peat; correspondingly the Gram-positive to Gram-negative bacterial ratio decreased with increasing N input and was lower in 0-5 cm than in 5-10 cm peat. Total microbial abundance and total bacterial abundance were significantly higher in 0-5 cm peat than in 5-10 cm peat. It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal bog microbial community responses to N loading.
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015
Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a poor fen near Mariana Lake, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha-1 yr-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. Fungal abundance increased at N addition levels above 16.6 kg N ha-1 yr-1 and total bacterial abundance also increased at N addition levels above 17.1 kg N ha-1 yr-1, such that the fungal:bacterial ratio was not significantly affected by N addition. Total microbial, gram-negative bacterial, and actinomycete abundance also showed an apparent threshold responses to N addition at 16-17 kg N ha-1 yr-1 addition levels.It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal detailed fen microbial community responses to N loading.
Phospholipid fatty acids (PFLA) on decomposed litter: An evolutionary perspective on functional diversity in co-occuring willow(salix) species
Thirteen willow (Salix) species occur in southeastern Minnesota and often co-occur within the same wetlands. This high local diversity is challenging to explain since closely related species are often functionally similar and density-dependent interactions such as competition and susceptibility to pests and pathogens should limit their co-occurrence. However, if willow species are partitioning resources, or if they are phylogenetically structured so that closely related species rarely co-occur, then the impact of these density-dependent processes could be reduced. In this study, I examined the role of niche partitioning in maintaining local willow diversity by comparing species physiology in a greenhouse.
Phospholipid fatty acids (PFLA) on decomposed litter: Nitrogen limitation in decomposition
Modern agriculture and fossil fuel combustion contribute to the transfer of N from largely inert pools (atmospheric N2, fossil fuel reserves) to biologically reactive forms that can be transported downwind from agricultural or industrial areas to ecosystems that historically may have experienced low levels of N inputs. Understanding how increased N inputs alter the cycling of another biologically important element, C, has been impeded by uncertainties about N effects on the process of decomposition. To date, ecologists remain unable to predict when, where, and in what forms N addition stimulates rates of decomposition. For example, recent work showed that in eight low-N sites in Central Minnesota, litter N was positively correlated with decomposition, suggesting N limitation of decomposition, yet addition of inorganic N fertilizer increased decomposition in only two of eight sites. These paradoxical results call into question the assumption that the often-observed correlation between substrate N concentration and decomposition arises because N limits decomposition. Research is addressing three interrelated questions:* (1) Why do litter N and externally supplied N have contrasting effects on decomposition in low-N ecosystems? (2) Do different forms of N (organic vs. inorganic; substrate vs. externally supplied) affect the activity, function and composition of the decomposer community differently, and, if so, what are the consequences for decomposition? (3) What are temporal dynamics of the activity, function, and composition of the decomposer community and do these dynamics depend upon the amount and forms of N supplied to the decomposer community?* These questions will be addressed using a 4-y decomposition experiment manipulating the quantity and form of N available to decomposers via use of substrates ranging in N concentrations and of inorganic (ammonium nitrate) and organic (amino acids) N fertilizers. The response of microbial biomass, stoichiometry, efficiency
SGS-LTER Graduate Student Research: Phospholipid fatty acid (PFLA) as Biochemical Responses of US Great Plains Grasslands to Regional and Interannual Variability in Precipitation (1999-2001)
This data package was produced by researchers working on the Shortgrass Steppe Long Term Ecological Research (SGS-LTER) Project, administered at Colorado State University. Long-term datasets and background information (proposals, reports, photographs, etc.) on the SGS-LTER project are contained in a comprehensive project collection within the Digital Collections of Colorado (http://digitool.library.colostate.edu/R/?func=collections&collection_id=3429). The data table and associated metadata document, which is generated in Ecological Metadata Language, may be available through other repositories serving the ecological research community and represent components of the larger SGS-LTER project collection. Additional information and referenced materials can be found: http://hdl.handle.net/10217/85531. Carbon (C) sequestration potential in grasslands is thought to be high due to the large soil organic carbon pools characteristic of these ecosystems. Inputs of C (aboveground net primary productivity) are highly correlated to precipitation across the Great Plains region; however, changes in C pool size at a specific site are governed by the relative input and output rates across time. Our objective was to quantify the ecosystem C response of three grassland community types (shortgrass steppe, mixed grass and tallgrass prairie) to interannual variation in precipitation. At five sites across a precipitation gradient in the Great Plains, we measured net primary production (NPP), soil respiration (SRESP), and litter decomposition rates for three consecutive years. NPP, SRESP, and litter decomposition increased from shortgrass steppe (175, 454, and 47 g C m-2 yr-1) to tallgrass prairie (408, 1221, and 348 g C m-2 yr-1 for NPP, SRESP, and litter decomposition respectively). Increased growing season precipitation between study years resulted in increased NPP, SRESP, and litter decomposition at almost all sites. However, the regional patterns of the interannual NPP, SRESP, and lit
Time Course Change in Skeletal Muscle and Blood Phospholipid Composition With Omega-3 Fatty Acid Supplementation
ClinicalTrials.gov study NCT05971147. IPD Sharing: NO. Countries: 1. Publications: 0.
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