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6 results for “photoconversion”
Dataset for "Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms"
<p>Dataset that supports the observation, characterization and application of the blue-shift photoconversion of the near infrared proteins, miRFPs, reported in the manuscript: "Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms". The data references to the specific figures and graphs in the manuscript.</p>
Long-Lived Ensembles of Shallow NV− Centers in Flat and Nanostructured Diamonds by Photoconversion
<p>Shallow, negatively charged nitrogen-vacancy centers (NV−) in diamond have been proposed for high-sensitivity magnetometry and spin-polarization transfer applications. However, surface effects tend to favor and stabilize the less useful neutral form, the NV0 centers. Here, we report the effects of green laser irradiation on ensembles of nanometer-shallow NV centers in flat and nanostructured diamond surfaces as a function of laser power in a range not previously explored (up to 150 mW/μm2). Fluorescence spectroscopy, optically detected magnetic resonance (ODMR), and charge-photoconversion detection are applied to characterize the properties and dynamics of NV− and NV0 centers. We demonstrate that high laser power strongly promotes photoconversion of NV0 to NV− centers. Surprisingly, the excess NV− population is stable over a timescale of 100 ms after switching off the laser, resulting in long-lived enrichment of shallow NV−. The beneficial effect of photoconversion is less marked in nanostructured samples. Our results are important to inform the design of samples and experimental procedures for applications relying on ensembles of shallow NV− centers in diamond.</p>
The giant clam photosymbiosis is a physically optimized photoconversion system for the most intense sunlight on Earth
<p>Giant clams are photosymbiotic with unicellular algae ("zooxanthellae") organized in the clam's mantle tissue. This tissue has an especially low albedo for a photosynthetic system, generally less than 10\% at all visible wavelengths. This efficient absorbance of light occurs in the ecological context of the high solar irradiances in intertidal habitats near the equator. At these light levels, photosynthetic systems typically adapt to absorb less light in order to prevent radiative damage to chloroplasts. Giant clams are therefore unusual. If the giant clam photosymbiosis proves to be simultaneously efficient at absorption and at phototransduction at these irradiances, they are potentially remarkably productive and an important source of bioinspiration. We showed previously that the clams organize algae into vertical pillars in the mantle tissue. The clams' iridocytes, or optically structured skin cells on the surface of the tissue, then function to evenly distribute incoming solar irradiance along the vertical faces of the pillars. The result is that zooxanthellae in the system absorb solar power at lower rates than that of incoming solar flux. The overall energetic performance of this phtooconversion scheme has, however, been difficult to characterize given the complex three-dimensional structure and the fact that it is coupled to a much more voluminous, respiring animal. Here we use a combination of photochemical characterization and new quantitative modeling of data from the literature to estimate the photochemical efficiency as a function of incoming irradiance of the initial electron-transfer events. Our approach is to consider the clam mantle tissue in isolation as a meta-material for photoconversion. To do this, we developed a method to directly measure the system's photochemical efficiency with spatial resolution of 10's of microns using optical microprobes threaded through the tissue. These experimental efficiency data then serve as ground-truthing for a subsequent reanalysis of photosynthesis-irradiance curves of clams taken from the literature. For this quantitative re-analysis, we incorporated the clam system's quantum efficiency as a function of irradiance per cell into a Monte Carlo model of radiative transfer among cells to find the tissue's area-specific oxygen evolution apart from any sinks. We found that cells located within the dense clam system had fluorescence transients (i.e., Kautsky curves), a direct measure of the efficiency of PS II) that were very slow and of low intensity, particularly for a dense system, consistent with photochemical efficiencies generally greater than 50\% and often greater than 90\%. When incorporated into a larger computational model, we found that mature Tridacnid clams can efficiently perform photoconversion of light energy into chemical energy at light intensities many times more intense than the maximum time-averaged environmental radiance, or even the solar constant. The intensities to which the clam is adapted, however, can be found in strong wave-lensed pulses of irradiance that are characteristic of the clams' habitats. This surprising result makes sense if the system has evolved to both avoid damage from and utilize the power in the intense pulses of light that result from wave-lensing. Our model predicts that by evolving to compensate for the intense pulses of solar energy produced by wave-lensing, the clam system can perform photochemical conversion of radiation at intensities many times greater than the solar constant at around 90\% quantum efficiency. This result in turn suggests a strategy for organic, engineered materials performing photoconversion under solar concentration.</p>
The giant clam photosymbiosis is a physically optimized photoconversion system for the most intense sunlight on Earth
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Data and visualisation code from 'Effects and avoidance of photoconversion-induced artefacts in confocal and STED microscopy' by Dasgupta et al (2024)
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Photoconversion of MC38
GEO Series GSE193654. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.