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5 results for “physiological buffering”
Data for dual behavioral-physiological buffering of mothers' milk
<p>This file (Ariaal_dual_buffering_data) contains the dataset used for the journal article manuscript entitled, "Dual behavioral-physiological buffering of mothers’ milk facilitate drought adaptability of pastoralists and agropastoralists in northern Kenya" by Fujita, Wander, Straight, and Wamwere-Njoroge. This manuscript is under review for publication as of August, 2024. The variables and data are found under the data tab, and the data coding information in the info & code tab.</p> <p> </p> <p>Please contact Masako Fujita (ORCID 0000-0001-9173-6678, E-mail masakof@msu.edu) for questions regarding the data.</p> <p> </p> <p>Condition for data use: Please cite DOI (DOI: 10.5281/zenodo.13285825) for this data file and the article, and acknowledge the support from the grant agencies listed below:</p> <p>Data source/article:</p> <p>Fujita M. 2024. Data for dual behavioral-physiological buffering of mothers' milk. DOI: 10.5281/zenodo.13285825.</p> <p>Fujita M, Wander K, Straight B, Wamwere-Njoroge G. [Year] Dual behavioral-physiological buffering of mothers’ milk facilitate drought adaptability of pastoralists and agropastoralists in northern Kenya. [Journal name, article DOI].</p> <p>Grant support:</p> <p> National Science Foundation (BCS-0622358, BCS-1638167)</p> <p> Wenner-Gren Foundation (Gr. 7460, Gr. 9278)</p>
A gas-only bioreactor system maintains stable culture environments and reveals that moderate pH deviations trigger transcriptome-wide responses in human cells cultured in physioxia and physiological buffers
<p><span>Although pH instability is emerging as a potential driver of changes in cell physiology, pH is still poorly controlled during cell culture and in vitro experiments. Standard procedures include the use of chemicals usually not present in the primary physiological buffering system (CO<sub>2</sub>/HCO<sub>3</sub><sup>-</sup>), such as acids and bases, to manipulate pH levels. This, however, leads to artifacts that potentially affect scientists' findings. </span><span>Here, we propose a novel method for controlling pH levels by relying only on the physiological buffering system. pH was manipulated in a repurposed commercial bioreactor set-up, using a two-sided control loop of CO<sub>2</sub> and N<sub>2</sub> gas in NaHCO<sub>3</sub>--buffered medium. This method produces optimal and stable dO<sub>2</sub> </span><span>profiles and tightly maintains pH levels. With this procedure, we analyzed the effects of different pH levels (6.8, 7.0, 7.2, and 7.4) on the performance and transcriptome of the human GM12878 cell line over a 72-hours experiment. Our results showed that inflammation and negative cellular proliferation are among the signatures activated at low pH. This further highlights the importance of a thorough pH control during cell culture. </span></p>
Data from: Amelioration of ocean acidification and warming effects through physiological buffering of a macroalgae
<p>Concurrent anthropogenic global climate change and ocean acidification is expected to have a negative impact on calcifying marine organisms. While knowledge of biological responses of organisms to oceanic stress has emerged from single species experiments, these do not capture ecologically relevant scenarios where the potential for multi-organism physiological interactions is assessed. Marine algae provide an interesting case study, as their photosynthetic activity elevates pH in the surrounding microenvironment, potentially buffering more acidic conditions for associated epiphytes. We present findings that indicate increased tolerance of an important epiphytic foraminifera, <em>Marginopora vertebralis</em>, to the effects of increased temperature (±3 °C) and pCO<sub>2</sub> (~1000 µatm) when associated with its common algal host, <em>Laurencia intricata</em>. Specimens of <em>M. vertebralis </em>were incubated for 15 days in flow-through aquaria simulating current and end-of-century temperature and pH conditions. Physiological measures of growth (change in wet weight), calcification (measured change in total alkalinity in closed bottles), photochemical efficiency (<em>Fv/Fm</em>), total chlorophyll, photosynthesis (oxygen flux), and respiration, were determined. When incubated in isolation, <em>M. vertebralis </em>exhibited reduced growth in end-of-century projections of ocean acidification conditions, while calcification rates were lowest in the high-temperature, low-pH treatment. Interestingly, association with<em> L. intricata</em> ameliorated these stress effects with the growth and calcification rates of<em> M. vertebralis </em>being similar to those observed in ambient conditions. Total chlorophyll levels in <em>M. vertebralis</em> decreased when in association with <em>L. intricata</em>, while maximum photochemical efficiency increased in ambient conditions. Net production estimates remained similar between <em>M. vertebralis </em>in isolation and in association with <em>L. intricata</em>, although both production and respiration rates of<em> M. vertebralis</em> were significantly higher when associated with <em>L. intricata</em>. These results indicate that the association with <em>L. intricata</em> increases the resilience of <em>M. vertebralis</em> to stress, providing one of the first examples of physiological buffering by a marine alga that can ameliorate the negative effects of changing ocean conditions.</p>
A gas-only bioreactor system maintains stable culture environments and reveals that moderate pH deviations trigger transcriptome-wide responses in human cells cultured in physioxia and physiological buffers
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Data from: Amelioration of ocean acidification and warming effects through physiological buffering of a macroalgae
Open the record for dataset details and reuse information.
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