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39 results for “phytohormone”
Root phytohormone levels of mangrove seedlings grown in soils of low and high bulk density
<p>These data contain information on root traits and concentrations of multiple hormones in root tissue of two mangrove species grown under low and high soil bulk density conditions. Inhibitors were used to assess the role of the phytohormone ethylene.</p> <h2>Description of the data and file structure</h2> <p>The root trait file contains total root length (cm), mean root length (cm), root dry weight (g), and root number data for two mangrove species (Avicennia marina, Rhizophora stylosa) grown under two different soil bulk densities: 0.2 and 1.0 g cm^-3. Treatment refers to the application of ethylene inhibitors (CoCl2: cobalt chloride, AIB: aminoisobutyric acid, control).</p> <p>The phytohormone file contains the root tissue concentrations (all in ng g^-1) for multiple hormones (ABA: abscisic acid, ACC: 1-aminocyclopropane-1-carboxylic acid, GAx: gibberellins, IAA: indole-3-acetic acid, iP: isopentenyl adenine, JA: jasmonic acid, SA: salicylic acid, tZ: cytokinin <em>trans</em>-zeatin) of two mangrove species (Avicennia marina, Rhizophora stylosa) grown under two different soil bulk densities: 0.2 and 1.0 g cm^-3. Treatment refers to the application of ethylene inhibitors (CoCl2: cobalt chloride, AIB: aminoisobutyric acid, control).</p> <p> </p> <p> </p>
Fig. 2 in The Impact Of Phytohormones On Pasqueflower (Pulsatilla) Regeneration In Vitro
Fig. 2. Induction of new rosettes forming when explants are transferring into MS media without phytohormones
Data from: Enhanced competitive advantage of invasive plants by growth-defense trade-off: Evidence from phytohormone metabolism and transcriptomic analysis
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Data from the study: Effect of experimental DNA demethylation on phytohormones production and palatability of a clonal plant after induction via jasmonic acid
<p>Many plant species protect themselves against herbivores through mechanical or chemical so-called inducible defences (ID). These are regulated via a hormonal cascade which may be under epigenetic control and in which jasmonic acid (JA) plays a prominent role.</p> <p>In this study, we indirectly tested the role of DNA methylation in the production of ID and the synthesis of hormones involved in the ID signalling cascade. Using different intensities of 5-azacytidine application, we aimed to produce plants of <i>Trifolium repens</i> with different levels of DNA methylation alteration. We then elicited the plants together with controls, i.e. plants with natural DNA methylation status, with JA and then indirectly recorded ID production in herbivore-choice trials in which the leaves of plants with different DNA methylation statuses were provided to caterpillars of a generalist herbivore, <i>Spodoptera littoralis.</i></p> <p>We also analysed the balance of several key defence hormones such as jasmonates, abscisic acid (ABA), indole-3-acetic acid (IAA) and salicylic acid in the plants. We found that the <i>Spodoptera littoralis</i> preferred demethylated plants over non-demethylated controls. Demethylation also reduced production of JA, ABA and IAA. We conclude that DNA methylation modulates expression of ID likely via regulation of signalling hormones involved in the establishment of defence.</p>
Phytohormone profiling in an evolutionary framework
<p>This repository contains raw LC/MS-acquired data for the study 'Phytohormone profiling in an evolutionary framework'.</p> <p>For sample codes see an attached excel file ('_LCMS-sample-codes.xlsx') at the end of the list.</p> <p>Phytohormone analysis was performed with a LC/MS system consisting of UHPLC 1290 Infinity II (Agilent, Santa Clara, CA, USA) coupled to 6495 Triple Quadrupole Mass Spectrometer (Agilent, Santa Clara, CA, USA), operating in MRM mode, with quantification by the isotope dilution method. Data acquisition and processing was performed with Mass Hunter software B.08 (Agilent, Santa Clara, CA, USA).</p>
Fig. 4 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 4. TT motif is not required for the production of ent-kaurene (1). Extracted ion (m/z = 272) chromatograms demonstrating the production of 1 by all mutants (as indicated) by comparison to wild-type (WT) AtKS.
Fig. 5 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 5. TT motif has minimal effect on catalytic efficiency. Curve fit of Michaelis-Menton equation to data for wild-type (WT) and indicated mutants of AtKS.
Fig. 3 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 3. Identification of the TT motif. A) Representative alignment of KSs spanning plant evolution with the KS specific pair of threonines (TT motif) indicated by asterisks (*) above the alignment. B) Sequence logos demonstrating the absolute conservation of this TT motif in KS (bottom) relative to the derived KS(L)/TPS-e subfamily more generally (top). C) Location of TT motif in active site from model of AtKS (blue) containing 2-fluoroGGPP (green) and Mg2+ co-factors (magenta) derived from the template co-crystal structure of taxadiene synthase (Koksal et al., 2011).
Fig. 2 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 2. Representative phylogenetic tree for plant KS(L)/TPS-e subfamily. Green lines indicate KS activity, red lines KSL that mediate alternative product outcome. Green text indicates KS with known or assumed role in phytohormone biosynthesis, while blue text indicates enzymes known to produce 1 for secondary metabolism – i.e., in maize (Fu et al., 2016).
Fig. 7 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 7. TT motif codons are not conserved. A) Exon sizes from KS with known genomic sequence. B) Sequence of TT motif containing exon from AtKS (codons for TT motif in larger blue text). C) Sequence logo indicating lack of conservation of the codons for the TT motif – i.e., in the variable third/'wobble' position.
Fig. 6 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 6. TT motif has minimal effect on protein structure. CD spectra for wildtype (WT) and the T527 V/T528V (TT/VV) double mutant of AtKS (note that the minimal differences observed here are not reproducible).
Fig. 5 in Magnetic Ti C MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry
Fig. 5. Spatio-temporal distribution of target phytohormones in different tissue of rapeseed germination.
Fig. 2 in Magnetic Ti C MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry
Fig. 2. XRD spectrum (a), FI-TR pattern (b), Raman spectrum (c) of the composite material, and magnetization hysteresis loop of Fe3O4@Ti3C2@β-CD (d).
Fig. 1 in Magnetic Ti C MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry
Fig. 1. Schematic of the synthetic route for Fe3O4@Ti3C2@β-CD and the sample pre-treatment procedure.
Fig. 4 in Magnetic Ti C MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry
Fig. 4. Effects of different cleanup sorbents (a), effects of the amount of magnetic solid-phase extraction sorbents (b), effects of the simultaneous derivatization and magnetic solid phase extraction time (c), effects of the desorption time (d). 5 mg rapeseed spiked with 10 ng/g of each analyte.
Fig. 3 in Magnetic Ti C MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry
Fig. 3. SEM image of Ti3C2 (a) and Fe3O4@Ti3C2@β-CD (b), TEM image of Ti3C2(c) and Fe3O4@Ti3C2@β-CD (d), elemental mapping and chemical composition of Fe3O4@Ti3C2@β-CD (e).
Data from the study: Effect of experimental DNA demethylation on phytohormones production and palatability of a clonal plant after induction via jasmonic acid
Open the record for dataset details and reuse information.
Fig. 1 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 1. KS reaction mechanism.
Cryptochrome 1 regulates growth and development in Brassica through alteration in the expression of genes involved in light, phytohormone and stress signalling
GEO Series GSE26797. Brassica napus; Brassica juncea. 4 samples. Type: Expression profiling by array.
An RNA-Seq atlas of gene expression in rose flower under treatment of various phytohormones and plant growth regulators
GEO Series GSE140696. Rosa hybrid cultivar. 39 samples. Type: Expression profiling by high throughput sequencing.
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