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2 results for “plakin domain”
Diffraction images of a crystal of the spectrin repeats 7, 8, and 9 (SR7-SR9) of the plakin domain of human plectin (PDB code 5J1I)
<p>Diffraction images of crystals of a fragment of the plakin domain of human plectin that includes the spectrin repeats 7 to 9 (SR7-SR9).</p> <p>Images correspond to the dataset used to solve and refine the pdb entry 5J1I (http://www.rcsb.org/pdb/explore/explore.do?structureId=5J1I).</p> <p>Data were collected on a single crystal at the beamline 14.2 of the European Synchrotron Radiation Facility (ESRF, Grenoble, France) using radiation of 0.9330 Å wavelength and an ADSC Q4 CCD detector. The dataset consists of 360 images (1 degree oscillation per image).</p> <p>Diffraction data is highly anisotropic. Based on analysis with the STARANISO server (http://staraniso.globalphasing.org/) data extend approximately to 5.0, 3.8, and 2.6 Å resolution along the three principal directions of anisotropy, which are 0.555 a*+ 0.832 c*, b*, and -0.361 a* + 0.932 c*, respectively.</p>
Diffraction images of crystals of the spectrin repeats 7 and 8 (SR7-SR8) of the plakin domain of human plectin (PDB code 5J1G): native and Hg-derivative datasets for phasing by SIRAS
<p>Diffraction images of crystals of a fragment of the plakin domain of human plectin that includes the spectrin repeats 7 to 8 (SR7-SR8).</p> <p>Images correspond to the dataset used to solve and refine the pdb entry <strong>5J1G</strong> (http://www.rcsb.org/pdb/explore/explore.do?structureId=5J1G).</p> <p> </p> <p>The structure was phase by single isomorphous replacement with anomalous scattering (SIRAS) using two datasets: one from a native crystal and another one from a crystal derivatized with the mercurial compound ethylmercurithiosalicylate (EMTS).</p> <p> </p> <p>The <strong>Native dataset</strong> was collected on a single crystal at the beamline XALOC of the ALBA Synchrotron (Barcelona, Spain) using radiation of 0.9792 Å wavelength and a PILATUS 6M detector. The dataset consists of 4 wedges of 450 images each (0.2º oscillation per image). Each wedge was collected at a different position of the same crystal. The crystals belong to the space group P2<sub>1</sub> with approximate cell dimensions <em>a</em>=45.7 Å, <em>b</em>=115.9 Å, <em>c</em>=64.8 Å, beta=97.6 º.</p> <p> </p> <p>The data from a <strong>mercurial derivative</strong> (EMTS) was collected in house using a rotating anode X-ray generator (wavelength 1.54179 Å) and a mar345 image plate detector. The dataset consists of 360 images (1º oscillation per image). The crystal was isomorphic to the native crystal.</p> <p> </p> <p>In addition to the diffraction images the following files are included:</p> <p>a) Files for indexing with the program XDS and the HKL files containing the integrated intensities.</p> <p>b) Files for scaling using the program xscale (directory XSCALE_5J1G_Native_EMTS).<br> c) The directory “phasing_shelx” contains hkl files of the intensities of the native and EMTS datasets in a format suitable for analysis with Shelx. This directory also contains the files of the phasing by SIRAS using Shelx C/D/E.</p>
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