Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
101
datasets available to search
ShareScore release 0.7.1
Dataset results
101 results for “plant immunity”
Genome-wide identification of cell-surface and intracellular immune receptors in 350 plant species
<p>Here we identified cell-surface (LRR-RLKs, LRR-RLPs, LysM-RLKs and LysM-RLPs) and intracellular immune receptors (NB-ARCs) from the genomes of 350 plant species. </p> <p> </p> <p>Zip file contains:</p> <p>Folder 'Immune_receptor_sequences' - FASTA files of the identified LRR-RLPs, Lys-RLKs, LysM-RLPs and NB-ARCs.</p> <p>Folder 'RLK_sequences' - FASTA files of the identified LRR-RLKs (all and 20 individual subgroups).</p> <p>Folder 'RLK_trees' - Phylogenetic TREE files of the identified LRR-RLKs (all and 20 individual subgroups); classified according to their kinase domains.</p> <p>238.species - Phylogenetic tree of the 238 plant species used in the analyses (taken from <a href="https://doi.org/10.1093/jpe/rtv047">https://doi.org/10.1093/jpe/rtv047</a>).</p> <p>350.species - Phylogenetic tree of the 350 plant species used in the analyses.</p> <p>simple.to.original.ids- Translator file for the original ID of each gene. </p> <p> </p>
Dataset for "Intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites"
<p>Datasets for preprint (https://doi.org/10.1101/2020.07.10.190785) entitled:</p> <p>"Intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites"</p> <p>Contains:</p> <p>- All data files for scripts quoted in the preprint and deposited at https://github.com/krasileva-group/hvNLR</p> <p>- Clade Membership Tables</p> <p>- Clade Alignment Files</p> <p>- Clade Trees</p> <p>- Excel files for Figure S1, Figure S2, and Table 1</p>
Fig. 1 in Protease inhibitors of fodder plants as a factor of immune response influencing the physiological state of the potato ladybird beetle Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae)
Fig. 1. Analysis of the population of the potato ladybird beetle with the species-specific PCR-markers of the gene COI mtDNA. А – species-specific marker for H. vigintioctopunctata, 400 b.p.; Б – species-specific marker for H. vigintioctomaculata, 406 b.p.; М – marker of the lengths of fragments 100 b.p. ladder; 1–3 – Primorsky krai: Chuguevsky district; 4–6 – Amurskaya oblast; 7–17 – Primorsky krai: Timiryazevsky.
Fig. 3 in Protease inhibitors of fodder plants as a factor of immune response influencing the physiological state of the potato ladybird beetle Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae)
Fig. 3. Sinergetic activity of the protainases of trypsin type (in an insect) and trypsin inhibitors (in a plant) in the course of feeding on different potato varieties.
Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity
<p><span>Encasements formed around haustoria and biotrophic hyphae as well as hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. In this study we examine the components that may contribute to the absence of these responses in susceptible barley attacked by the powdery mildew fungus. We find that the effector CSEP0162 from this pathogen targets plant MONENSIN SENSITIVITY1 (MON1), which is important for the fusion of multivesicular bodies to their target membranes. Overexpression of CSEP0162 and silencing of barley MON1 both inhibit encasement formation. We find that the Arabidopsis ecotype No-0 has resistance to powdery mildew, and that this is partially dependent on MON1. Surprisingly, we find the MON1-dependent resistance in No-0 not only includes an encasement response, but also an effective HR. Similarly, silencing of MON1 in barley also blocks Mla3-mediated HR-based powdery mildew resistance. Our results indicate that MON1 is a vital plant immunity component, and we speculate that the barley powdery mildew fungus introduces the effector CSEP0162 to target MON1 and hence reduces encasement formation and HR.</span></p>
Plant species with higher chemical defenses enhance herbivore cellular immunity with differential effectiveness against two parasitoid species
<p>Insect herbivores simultaneously experience bottom-up effects of plant defensive chemistry and the top-down effects of natural enemies. At the intersection of these effects are herbivore immune systems, herbivore traits that have largely been overlooked in studies of plant-insect interactions. Most previous studies have demonstrated compromised immunity of herbivores that feed on plants with higher defensive chemistry. Many studies have used embedded microfilaments or silica beads as proxies for parasitoid eggs. Yet, parasitoids may evade or suppress host immune responses by injecting venom, calyx fluid, or through modifications of their egg surface structure, necessitating studies that include all three trophic levels to obtain a complete picture of how plant traits may modulate herbivore immunity.Here we examined the effect of host plant species that differ in glucosinolate (anti-herbivore compounds produced by plants in the Brassicaceae) concentrations on the immune status of an herbivore and its consequences for two species of parasitoids with different life history traits.We found that larvae of the butterfly Pieris rapae that fed on field mustard Brassica rapa, which contain 52-fold higher glucosinolate concentrations than collards B. oleracea, attained lower body weights and experienced prolonged development to adulthood.Yet, caterpillars that fed on B. rapa had enhanced cellular immunity, as measured by total and differential hemocyte counts, as well as melanization capacity compared to larvae that fed on B. oleracea.In turn, the likelihood that at least some eggs in clutches of the gregarious endoparasitoid Cotesia glomerata would be encapsulated, leading to a reduction in brood size, were three times greater when their host caterpillars fed on B. rapa compared to B. oleracea. Interestingly, eggs of the solitary endoparasitoid Cotesia rubecula were rarely encapsulated irrespective of the host plant on which their host caterpillar fed. Therefore, our results suggest that plant defense metabolites can influence the expression of herbivore immunity, but the effectiveness of this response strongly depends on the identity of the parasitoid and its ability to evade the caterpillar immune response, and possibly the evolution of these trophic interactions in non-native systems.</p>
Glutathione and neodiosmin feedback sustain plant immunity
<p>Plants have evolved a two-layer immune system comprising pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) that is activated in response to pathogen invasion. Microbial patterns and pathogen effectors can be recognized by surface-localized pattern-recognition receptors (PRRs) and intracellularly localized nucleotide-binding leucine-rich repeat receptors (NLRs) to trigger PTI and ETI responses, respectively. At present, the metabolites activated by PTI and ETI and their roles and signalling pathways in plant immunity are not well understood. In this study, metabolomic analysis showed that ETI and PTI induced various flavonoids and amino acids and their derivatives in plants. Interestingly, both glutathione and neodiosmin content were specifically up-regulated by ETI and PTI, respectively, which significantly enhanced plant immunity. Further studies showed that glutathione and neodiosmin failed to induce a plant immune response in which PRRs/co-receptors were mutated. In addition, glutathione-reduced mutant gsh1 analysis showed that GSH1 is also required for PTI and ETI. Finally, we propose a model in which glutathione and neodiosmin are considered signature metabolites induced in the process of ETI and PTI activation in plants and further continuous enhancement of plant immunity in which PRRs/co-receptors are needed. This model is beneficial for an in-depth understanding of the closed-loop mode of the positive feedback regulation of PTI and ETI signals at the metabolic level.</p>
The plant toxin 4-methylsulfinylbutyl isothiocyanate decreases herbivore performance and modulate cellular and humoral immunity
<p class="MsoNormal"><span>Insect herbivores frequently encounter plant defense molecules, but the physiological and ecological consequences for their immune systems are not fully understood. The majority of studies attempting to relate levels of plant defensive chemistry to herbivore immune responses have used natural population or species-level variation in plant defensive chemistry. Yet, this potentially confounds the effects of plant defense chemistry with other potential traits that may affect the expression of herbivore immunity such as development time and nutritional quality. We have used an artificial diet containing known quantities of a plant toxin (4-methylsulfinylbutyl isothiocyanate; 4MSOB-ITC or ITC), an isothiocyanate present in many plants in the genus <em>Brassica</em>, to explicitly explore the effects of a plant toxin on the cellular and humoral immune responses of the generalist herbivore <em>Trichoplusia ni</em> (Lepidoptera: Noctuidae) that frequently feeds on glucosinolate-containing plants. Caterpillars feeding on diets with high concentrations of ITC experienced reduced survivorship and growth rates. High concentrations of ITC suppressed the appearance of several types of hemocytes and melanization activity, which are critical defenses against parasitic Hymenoptera and microbial pathogens. In terms of </span><em><span>T. ni</span></em><span> humoral immunity, only </span><span>the antimicrobial peptide (AMP) genes <em>lebocin</em> and <em>gallerimycin </em>were significantly upregulated in caterpillars fed on diets </span><span>containing high levels of 4MSOB-ITC relative to caterpillars that were provided with ITC-free diet</span><span>. Surprisingly, challenging </span><span>caterpillars</span><span> </span><span>with a non-pathogenic strain of </span><em><span>Escherichia coli</span></em><span> resulted in the upregulation of the AMP gene <em>cecropin</em>. Feeding on high concentrations of plant toxins hindered caterpillar development and decreased cellular immunity but conferred mixed effects on humoral immunity. Our findings provide novel insights into the effects of herbivore diet composition on insect performance demonstrating the role of specific plant defense toxins that shape herbivore immunity and trophic interactions. </span></p>
The N-terminal executioner domains of NLR immune receptors are structurally and functionally conserved across major plant lineages: Extended Data
<p>Raw data and supporting files for an updated version of the manuscript now entitled "The N-terminal executioner domains of NLR immune receptors are structurally and functionally conserved across major plant lineages".</p> <p>Related to an original version of the bioRxiv preprint: https://www.biorxiv.org/content/10.1101/2022.10.19.512840v1 </p>
Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity
Open the record for dataset details and reuse information.
Plant species with higher chemical defenses enhance herbivore cellular immunity with differential effectiveness against two parasitoid species
Open the record for dataset details and reuse information.
Glutathione and neodiosmin feedback sustain plant immunity
Open the record for dataset details and reuse information.
The plant toxin 4-methylsulfinylbutyl isothiocyanate decreases herbivore performance and modulate cellular and humoral immunity
Open the record for dataset details and reuse information.
Data from: Symbiotic polydnavirus of a parasite manipulates caterpillar and plant immunity
Obligate symbioses occur when organisms require symbiotic relationships to survive. Some parasitic wasps of caterpillars possess obligate mutualistic viruses called "polydnaviruses." Along with eggs, wasps inject polydnavirus inside their caterpillar hosts where the hatching larvae develop inside the caterpillar. Polydnaviruses suppress the immune systems of their caterpillar hosts, which enables egg hatch and wasp larval development. It is unknown whether polydnaviruses also manipulate the salivary proteins of the caterpillar, which may affect the elicitation of plant defenses during feeding by the caterpillar. Here, we show that a polydnavirus of the parasitoid Microplitis croceipes, and not the parasitoid larva itself, drives the regulation of salivary enzymes of the caterpillar Helicoverpa zea that are known to elicit tomato plant-defense responses to herbivores. The polydnavirus suppresses glucose oxidase, which is a primary plant-defense elicitor in the saliva of the H. zea caterpillar. By suppressing plant defenses, the polydnavirus allows the caterpillar to grow at a faster rate, thus improving the host suitability for the parasitoid. Remarkably, polydnaviruses manipulate the phenotypes of the wasp, caterpillar, and host plant, demonstrating that polydnaviruses play far more prominent roles in shaping plant–herbivore interactions than ever considered.
Immune assay data for Lycaeides melissa larvae reared on different host plants and with/without egg microbes
<p class="MsoListParagraphCxSpFirst"><span>1.<span> </span></span><span>Maternally transmitted microbes are ubiquitous. In insects, maternal microbes can play a role in mediating the insect immune response. </span><span>Less is known about how ecological factors, such as resource use, interact with maternal microbes to affect immunity. </span></p> <p class="MsoListParagraphCxSpMiddle"><span>2.<span> </span></span><span>In the context of a recent colonization of a novel host plant by the Melissa blue butterfly (<em>Lycaeides melissa</em>), we investigated the interaction between host plant use and vertically transmitted, extracellular egg-associated microbes in determining the strength of the insect immune response. </span></p> <p class="MsoListParagraphCxSpMiddle"><span>3.<span> </span></span><span>We reared larvae on two different host plant species: a native host <em>Astragalus canadensis</em> and a novel host <em>Medicago sativa</em>. Egg-associated microbes were removed through a series of antimicrobial egg washes prior to hatching. Immune response was measured through three assays: standing phenoloxidase (PO), total PO, and melanization. </span></p> <p class="MsoListParagraphCxSpMiddle"><span>4.<span> </span></span><span>We detected strong effects of microbial removal. Egg washing resulted in larvae with an increased immune response as measured by total PO- contrary to reports from other taxa. The effect of washing was especially strong for larvae consuming the native host plant. </span></p> <p> </p> <p class="MsoListParagraphCxSpLast"><span>5.<span> </span></span><span>This result may explain why consumption of the egg casing is not a universal behaviour in insects, due to negative effects on larval immunity. </span></p>
Data from: Variation in immune response in the generalist herbivore fall webworm across four common host plants
Open the record for dataset details and reuse information.
Crystal structure of the LRR ectodomain from the plant immune receptor kinase SOBIR1 from Arabidopsis thaliana - native dataset
<p>This dataset includes the raw X-ray diffraction images collected on 02.02.2018 at beam line PXIII of the Swiss Light Source (SLS) Villigen, Switzerland. The dataset includes a .bz2 archive of the XDS processing, the resulting XDS_ASCII.HKL contains the integrated intensities. The corresponding coordinates and crystallographic structure factors have been deposited with the Protein Data Bank (http://rcsb.org) with ID 6R1H.</p>
Crystal structure of the LRR ectodomain from the plant immune receptor kinase SOBIR1 from Arabidopsis thaliana - sulphur SAD datasets
<p>This dataset includes the raw X-ray diffraction images collected on 06.11.2017 at beam line PXIII of the Swiss Light Source (SLS) Villigen, Switzerland. The archive native_images.tar contains a native dataset to 1.75 Å resolution (λ=1.033201 Å, 1 360° wedge at 0.1° oscillation). The archive ssad_images.tar contains redundant sulphur single-wavelength anomalous dispersion (SAD) data (λ= 2.078524 Å, 3 360° wedges at 0.1° oscillation) to 3.12 Å resolution. The xds_nat_ssad.tar.bz2 includes a .bz2 archive of the XDS processing for native and sulphur SAD data. Data were scaled together in xscale, the resulting xscale.hkl and nat1.hkl contain the integrated intensities and crystallographic structure factors. The corresponding coordinates have been deposited with the Protein Data Bank (http://rcsb.org) with ID 6R1H.</p>
Engineering the plant intracellular immune receptor Sr50 to restore recognition of the AvrSr50 escape mutant
<p>The datasets for '<strong>Engineering the plant intracellular immune receptor Sr50 to restore recognition of the AvrSr50 escape mutant</strong><strong>'</strong>.</p> <p> </p> <p><strong>All_docking_models.zip</strong>: molecular docking models used to derive the initial structural hypothesis (Model I, Alternative models I and II). </p> <p><strong>Zdock1.AF2.zip</strong>: includes refined, relaxed structures of Model I. </p> <p><strong>Zdock5.AF2.zip</strong>: includes refined, relaxed structures of Alternative model II. </p> <p><strong>Zdock15.AF2.zip</strong>: includes refined, relaxed structures of Alternative moel I.</p> <p><strong>Model_II.zip</strong>: includes ColabDock outputs used as Model II.</p> <p><strong>Model_III.zip</strong>: includes ColabDock outputs used as Model III.</p> <p><strong>Model_IV.zip</strong>: includes ColabFold outputs used as Model IV.</p> <p><strong>Sr50*.AF.zip</strong>: predicted structures by ColabFold for the indicated pairs of the receptors and effectors. </p> <p> </p> <p>To read more about the workflow, please refer to https://github.com/s-kyungyong/Sr50-AvrSr50</p>
Data from: Use of an exotic host plant shifts immunity, chemical defense, and viral burden in wild populations of a specialist insect herbivore
<p>Defense against natural enemies constitutes an important driver of herbivore host range evolution in the wild. Populations of the Baltimore checkerspot butterfly, <em>Euphydryas phaeton </em>(Nymphalidae), have recently incorporated an exotic plant, <em>Plantago lanceolata </em>(Plantaginaceae), into their dietary range. To understand the tritrophic consequences of utilizing this exotic host plant, we examined immune performance, chemical defense, and interactions with a natural entomopathogen (Junonia coenia densovirus, <em>Parvoviridae</em>) across wild populations of this specialist herbivore. We measured three immune parameters, sequestration of defensive iridoid glycosides (IGs), and viral infection load in field-collected caterpillars using either <em>P. lanceolata</em> or a native plant, <em>Chelone glabra </em>(Plantaginaceae). We found that larvae using the exotic plant exhibited reduced immunocompetence, compositional differences in IG sequestration, and higher <em>in situ </em>viral burdens compared to those using the native plant. On both host plants, high IG sequestration was associated with reduced hemocyte concentration in the larval hemolymph, providing the first evidence of incompatibility between sequestered chemical defenses and the immune response (i.e., the "vulnerable host" hypothesis) from a field-based study. However, despite this negative relationship between IG sequestration and cellular immunity, caterpillars with greater sequestration harbored lower viral loads. While survival of virus-infected individuals decreased with increasing viral burden, it ultimately did not differ between the exotic and native plants. These results provide evidence that (1) phytochemical sequestration may contribute to defense against pathogens even when immunity is compromised, and (2) herbivore persistence on exotic plant species may be facilitated by sequestration and its role in defense against natural enemies.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.