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2 results for “point spread function”
Zeiss Lattice Light Sheet 7 Point Spread Functions and example dataset
<p>This repository contains some point spread functions (PSFs) distilled from imaging fluorescent beads, 200nm, <a href="https://www.thermofisher.com/order/catalog/product/F8811?SID=srch-srp-F8811" target="_blank" rel="noopener noreferrer">F8811</a> in an agarose gel, on a Zeiss Lattice Light sheet 7 microscope. The bead images from which the PSF were distilled are contained in this repo.</p> <p>Several images were acquired with varying settings of the light sheet (light sheet was always set as the 30 um x1000 nm).</p> <p>These three parameters were varied:</p> <p>FS: focus sheet (-100, -75, -25, 0)</p> <p>FW: focus waist (40, 45, 50, 55, 60, 65, 70, 75, 80, 85)</p> <p>AC: aberration control (150, 155, 160, 165, 170, 175, 180, 185, 190)</p> <p>The file psf-200nm correspond to the best settings taken between the different settings. The two other files (psf-300nm, psf-400nm) correspond to resampled versions of the psf-200nm file.</p> <p>The all-psfs.tif contains the deskewed psf ordered similarly as the poster uploaded in this version of the repository (ELMI-2024-poster.pdf).</p> <p>A sample czi file (Hela Kyoto, raw data) is provided to test deconvolution algorithms.</p> <p>Note that none of these data is deskewed.</p> <p>--------------------</p> <p>Here's a protocol to prepare a gel containing beads at a good enough density:</p> <ul> <li> <p>Material:</p> <ul> <li>Agarose 2%</li> <li>Fluorescent beads, 200nm, <a href="https://www.thermofisher.com/order/catalog/product/F8811?SID=srch-srp-F8811">F8811</a>. Stock is 1000x</li> </ul> </li> <li> <p>Protocol:</p> <ul> <li>Put the agarose to warm up at 90C.</li> <li>Vortex the beads stock.</li> <li>Dilute a 100 times the bead dilution in PBS (10 uL in 1mL), vortex.</li> <li>Prepare a clean slide, and a clean coverglass (22x22mm).</li> <li>Take 90uL of agarose, put in a 1.5mL tube, add 10uL of the diluted beads, vortex.</li> <li>Take 50uL of this agarose solution, put it on the slide, add the coverglass on top.</li> <li>Put this slide in the fridge and wait a few minutes.</li> <li>Use nailpolish to seal the coverglass. Wait long enoughfor the nailpolish to dry.</li> </ul> </li> </ul> <p>You should a gel of about 100um thickness.</p> <p>It should be possible to add a bit of free dye in the gel in order to help focusing the light sheet. Ideally a bit of far-red free dye, like Alexa 647.</p>
Reconstructed spatial resolution and contrast recovery with Bayesian penalized likelihood reconstruction (Q.Clear) for FDG-PET compared to time-of-flight (TOF) with point spread function (PSF)
<p>DICOM data and SPSS datasets with all derived measures (SUV, recovery coefficients, spatial resolution, SNR) that are the basis for the publication.</p>
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