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Figure 3 in On the successful acclimatization of the Colorado potato beetle Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) in Primorsky kray

Figure 3. Modelling of the ecological niches of the Colorado potato beetle for the Far Eastern, European, and North American habitats by the method of metric two-dimensional

opencc-by-4.0Jan 2024View details →

Fig. 1 in Protease inhibitors of fodder plants as a factor of immune response influencing the physiological state of the potato ladybird beetle Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae)

Fig. 1. Analysis of the population of the potato ladybird beetle with the species-specific PCR-markers of the gene COI mtDNA. А – species-specific marker for H. vigintioctopunctata, 400 b.p.; Б – species-specific marker for H. vigintioctomaculata, 406 b.p.; М – marker of the lengths of fragments 100 b.p. ladder; 1–3 – Primorsky krai: Chuguevsky district; 4–6 – Amurskaya oblast; 7–17 – Primorsky krai: Timiryazevsky.

opencc-by-4.0Jan 2024View details →

Fig. 3 in Protease inhibitors of fodder plants as a factor of immune response influencing the physiological state of the potato ladybird beetle Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae)

Fig. 3. Sinergetic activity of the protainases of trypsin type (in an insect) and trypsin inhibitors (in a plant) in the course of feeding on different potato varieties.

Figure 3 in On the successful acclimatization of the Colorado potato beetle Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) in Primorsky kray

Figure 3. Modelling of the ecological niches of the Colorado potato beetle for the Far Eastern, European, and North American habitats by the method of metric two-dimensional scaling using the Jaccard coefficient.

Figure 1 in On the successful acclimatization of the Colorado potato beetle Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) in Primorsky kray

Figure 1. Dynamics of the population size of the Colorado potato beetle over the year of the research (population peaks are shown on average for Primorsky Kray).

opencc-by-4.0Dec 2023View details →

Рис. 6. МоΔеΛирование экоΛогических ниш коΛораΔского жука ΔΛя ΔаΛьневосточного, европейского и североамериканского ареаΛов метоΔом метрического Δвухмерного шкаΛирования с применением коэффициента Жаккара Fig. 6. Models of ecological niches of the Colorado potato beetle for the Far Eastern, European, and North-American habitats (metric multidimensional scaling, Jaccard index) in Comparative characterization of the ecology of native (Henosepilachna vigintioctomaculata) and invasive (Leptinoatrsa decemlineata) species under the conditions of the monsoon climate in the southern part of the Russian Far East

Рис. 6. МоΔеΛирование экоΛогических ниш коΛораΔского жука ΔΛя ΔаΛьневосточного, европейского и североамериканского ареаΛов метоΔом метрического Δвухмерного шкаΛирования с применением коэффициента Жаккара Fig. 6. Models of ecological niches of the Colorado potato beetle for the Far Eastern, European, and North-American habitats (metric multidimensional scaling, Jaccard index)

opencc-by-4.0Jul 2024View details →

Рис. 1. КоΛичество макрокониΑий грибов роΑа Fusarium (% от общего чисΛа эΛементов морфоΛогии) на органах и в физиоΛогических жиΑкостях картофеΛьной коровки Fig. 1. Number of macroconidia of fungus species from the genus Fusarium (% of the total number of morphological elements) on organs and in physiological fluids of the potato ladybird beetle in On the vector characteristics of the potato ladybird beetle Henosepilachna Vigintioctomaculata (Motsch.) (Coleoptera, Coccinellidae) in the system "phytophagous insect - plant pathogen - plant"

Рис. 1. КоΛичество макрокониΑий грибов роΑа Fusarium (% от общего чисΛа эΛементов морфоΛогии) на органах и в физиоΛогических жиΑкостях картофеΛьной коровки Fig. 1. Number of macroconidia of fungus species from the genus Fusarium (% of the total number of morphological elements) on organs and in physiological fluids of the potato ladybird beetle

Figure 5 in Exploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)

Figure 5. Effect of dsRNA feeding on leaf consumption was assessed in L. decemlineata larvae at different instars: (a) third instar and (b) fourth instar, following the feeding assay. Bars indicate standard error (SE) on columns. Different letters on the columns denote significant differences determined by ANOVA followed by the Tukey HSD test at a 5% significance level.

Figure 4 in Exploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)

Figure 4. Influence of dsRNA ingestion on weight gain was calculated in the L. decemlineata larvae at different instars: (a) third instar and (b) fourth instar, following the feeding assay. Bars indicate standard error (SE) in columns. Different letters on the columns denote significant differences determined by ANOVA followed by the Tukey HSD test at a 5% significance level.

Figure 3 in Exploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)

Figure 3. Effect of the dsRNA feeding on the V-ATPase expression levels in the L. decemlineata larvae at different instar stages: (a) First instar, (b) second instar, (c) third instar, and (d) fourth instar. Bars indicate the standard error (SE) in the columns. Different letters on the columns denote significant differences determined by ANOVA followed by the Tukey HSD test at a 5% significance level.

Figure 2. Mortality percentages after feeding the L in Exploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)

Figure 2. Mortality percentages after feeding the L. decemlineata larvae dsRNA-treated leaves at different instars. (a) First instar, (b) second instar, (c) third instar, and (d) fourth instar. The percent mortality was compared in the larvae fed potato leaves pretreated with E. coli HT115 (DE3) cells expressing dsV-ATPase compared to the two controls, E. coli HT115 (DE3) cells with empty L4440 plasmid (dsEmp) and E. coli HT115 expressing dsGFP. Different letters on the data points denote significant differences determined by ANOVA followed by the Tukey honest significant difference (HSD) test at a 5% significance level.

opencc-by-4.0Jan 2023View details →

Utilizing traditional and remote sensing techniques to assess Colorado potato beetle host preference in the Columbia Basin -- 2021 Data

This is a remote sensing dataset collected in 2021 that contains orthomosaic images, shape files, analysis scripts, and derived numerical data from each plot. Data was collected using the protocol described here: <a href="https://www.protocols.io/view/usda-ars-potato-genetics-lab-drone-data-collection-bp2l6148dvqe/v1">https://www.protocols.io/view/usda-ars-potato-genetics-lab-drone-data-collection-bp2l6148dvqe/v1</a> Provided are "field map" files that denote the location and contents of each plot, a folder from each date that contains the 10 band orthomosiac, surface model image, a cropped and rotated image, shape files indicating the location of each plot, and derived data. The analysis can be replicated by following along with workflow listed in file named: rondon_cpb_2021.R. Derived data from this experiment can be found it the file named: "Rondon_CPB_data_2021_UAS_all.csv" If you have any questions or comments regarding this dataset please contact Dr. Max Feldman via email: max.feldman@usda.gov

dryad36/100

Data from: Cropland connectivity affects genetic divergence of Colorado potato beetle along an invasion front

The population genetic structure of invasive species can be strongly affected by environmental and landscape barriers to dispersal. Disentangling the relative contributions of these factors to genetic divergence among invading populations is a fundamental goal of landscape genetics with important implications for invasion management. Here, we relate patterns of genetic divergence in a global invasive agricultural pest, Colorado potato beetle (CPB; Leptinotarsa decemlineata), to environmental and landscape factors along an invasion front in Northwestern China. We first used microsatellite markers and spatial-temporal samples to assess broad patterns of genetic diversity as well as fine-scale changes in patterns of genetic divergence. We then distinguished the relative contributions of five factors to genetic divergence among front populations: geographic distance (isolation by distance), climate dissimilarity (isolation by environment), and least-cost distances (isolation by resistance) modelled with three factors: climate suitability, cropland cover, and road networks. Genetic diversity broadly decreased from West to East, with the exception being Eastern China. Low levels of genetic diversity and varying degrees of divergence were observed in Northwestern China, reflecting the potential effect of landscape heterogeneity. Least-cost distance across cropland cover was most positively correlated with genetic divergence, suggesting a role of croplands in facilitating gene flow. The contribution of climate to genetic divergence was secondary, whether modelled in terms of local adaptability or connectivity of the climatic landscape, suggesting that constraints to CPB gene flow imposed by a harsh climate may be ameliorated in agricultural landscapes. No evidence was found for an obvious effect of road networks on genetic divergence and population structuring. Our study provides an example of how agricultural landscape connectivity can facilitate the spread of invasive pests, even across a broad climatic gradient. More broadly, our findings can guide decisions about future land management for mitigating further spread.

opencc-zeroSep 2020View details →

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Elevated rates of positive selection drive the evolution of pestiferousness in the Colorado potato beetle ( Leptinotarsa decemlineata, Say)

In order to understand the evolution of pestiferousness, which we define as the accumulation of traits that contribute to an insect population's success in an agroecosystem, we tested the importance of known genomic properties associated with rapid adaptation. Within the leaf beetle genus Leptinotarsa, only the Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, and a few populations therein, has risen to pest status on cultivated nightshades, Solanum. Using whole genomes from ten closely related Leptinotarsa species native to the United States we reconstructed a high-quality species tree and used this phylogenetic framework to assess evolutionary patterns in four genomic features of rapid adaptation: standing genetic variation, gene family expansion and contraction, transposable element variation, and positive selection at protein coding genes. Throughout approximately 20 million years of history, Leptinotarsa species show little evidence of gene family turnover and transposable element variation. However, there is a clear pattern of recently derived lineages, including CPB, experiencing higher rates of positive selection on protein coding genes. We determine these rates are associated with greater standing genetic variation due to larger effective population size, which support the theory that the demographic history contributes to rates of protein evolution. Furthermore, we identify a suite of genes under positive selection that are linked to pestiferousness, exclusively, in the Colorado potato beetle lineage. They are involved in the biological processes of xenobiotic detoxification, chemosensation, and hormone function.

opencc-zeroJan 2021View details →

Fig. 2 in On the successful acclimatization of the Colorado potato beetle Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) in Primorsky kray

Fig. 2. Population pyramid of the Colorado potato beetle in Primorsky Kray.

Figure 1 in On the successful acclimatization of the Colorado potato beetle Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) in Primorsky kray

Figure 1. Dynamics of the population size of the Colorado potato beetle over the year of

opencc-by-4.0Jan 2024View details →

POBED - A POtato BEetle Dataset

Open the record for dataset details and reuse information.

dryad36/100

The role of structural variants in pest adaptation and genome evolution of the Colorado potato beetle, Leptinotarsa decemlineata (Say)

Structural variation has been associated with genetic diversity and adaptation in diverse taxa. Despite these observations, it is not yet clear what their relative importance is for microevolution, especially with respect to known drivers of diversity, e.g., nucleotide substitutions, in rapidly adapting species. Here we examine the significance of structural variants (SVs) in pesticide resistance evolution of the agricultural super-pest, the Colorado potato beetle, Leptinotarsa decemlineata. By employing a parent offspring trio sequencing procedure, we develop highly contiguous reference genomes to characterize structural variation within this species. These updated assemblies represent >100-fold improvement of contiguity and include derived pest and ancestral non-pest individuals. We identify >200,000 SVs, which appear to be non-randomly distributed across the genome as they co-occur with transposable elements and genes. SVs intersect exons for a large proportion of gene annotations (~20%) and are associated with insecticide resistance, development, and transcription, most notably cytochrome P450 (CYP) genes. To understand the role that SVs might play in adaptation we measure allele frequencies of SVs for an additional 57 individuals, using whole genome resequencing data, representing pest and non-pest populations of North America. Incorporating multiple independent tests of significance using SNP data, we identify 14 positively selected genes that include SVs and SNPs of elevated frequency within the sampled pest lineages. Among these, four are associated with insecticide resistance. One of these genes, glycosyltransferase-13, is a duplicated gene enclosed within a structural variant that resides inside the CYP4g15 genic region. Both gene products have been observed to be co-induced during insecticide exposure. These results demonstrate the significance of structural variations as a genomic feature to describe species history, genetic diversity, and adaptation.

opencc-zeroJun 2024View details →

opencc-by-4.0Jan 2023View details →

Utilizing traditional and remote sensing techniques to assess Colorado potato beetle host preference in the Columbia Basin -- Derived data 2020 & 2021

This is derived data from a remote sensing experiment performed in 2020 and 2021. This repository contains .csv and .R files that can be used to replicate the analysis presented here: <a href="https://zenodo.org/record/6859791#.Y-K6ky-B1z8">https://zenodo.org/record/6859791#.Y-K6ky-B1z8</a> If you have any questions or comments regarding this dataset please contact Dr. Max Feldman via email: max.feldman@usda.gov