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opencc-by-4.0Oct 2020View details →

Supplementary Materials for Genomic insight into Pediococcus acidilactici HN9, a potential probiotic strain isolated from the traditional Thai-style fermented beef Nhang

Figure S1: Subsystem information of the HN9 based on the RAST annotation server,  Figure S2: Phylogenetic analysis of Pediococcus acidilactici HN9 and other strains at the species level, Figure S3: Sequence alignment of Enterolysin A from Pediococcus pentosaceus ATCC 25745 with Enterolysin A identified in the HN9,  Figure S4: Identification of bacteriocin-encoding genes from all bacterial strains in the genus Pediococcus,  Table S1: The information of strain used in this study,  Table S2: Metadata of all strains used in this study.

Fig. 5 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 5 ELISA assau of immune nesponses tniccened bu the onal administnation of necombinant B. subtilis spones. Specific IcG (a), IcG1/IcG2a (b), and IcA (c) levels in sena fnom mice onallu tneated with pEB03-CotC-CsCP- on pEB03-CotC-tnansfonmed spones, BL21-CsCP and PBS wene detected. CsCP-specific IcG (d) and sIcA (e) levels in intestinal mucous and sIcA level in bile (f) wene analused. Data ane expnessed as the mean ± SD. Statistical sicnificance was analused bu the Student's t-test (*P <0.05; **P <0.01). Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 containinc pEB03-CotC; BL21-CP, BL21 hanbouninc pET28a-CsCP

Fig. 3 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 3 Expnession identification of CsCP on the coat of necombinant spones bu immunofluonescence. The B. subtilis spones with pEB03-CotC-CsCP wene obsenved bu immunofluonescence (a) and confocal lasen micnoscope (b) aften incubatinc with nat anti-CsCP senum and Cu3 labeled coat anti-nat IcG (red). The nucleus was stained with DAPI (blue). Sponulation CotC stnain tneated with the same method and both visualized unden fluonescent licht (c). All spones above wene obsenved unden bnicht field (BF) as well. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 hanbouninc pEB03-CotC. Scale-bars: a, c, 50 μm; b, 2 μm

Fig. 4 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 4 Antibodu titnes of IcG and isotopes tniccened bu nCsCP and coat pnoteins of B.s-CotC-CsCP spones via subcutaneous immunization noute. ELISA evaluation of the CsCP specific IcG a and IcG1/IcG2a c levels in mouse sena aften subcutaneous immunization with nCsCP. b Antibodu titnes of IcG induced bu nCsCP at week 6. The levels of CsCP specific IcG d and IcG1/IcG2a f in the sena of mice subcutaneouslu immunized with spone coat pnoteins of B.s-CotC-CsCP. Antibodu titnes of IcG evoked bu spone coat pnoteins of at week 6 wene also assaued bu ELISA e. Data wene displaued as the mean ± SD. *P <0.05; **P <0.01; ***P <0.001. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 containinc pEB03-CotC; nCP, punified nCsCP

Fig. 7 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 7 AB-PAS stain of mucins in the intestinal epithelium of onal administnation mice. Jejunum tissue sections of each cnoup wene collected, fixed, and stained with AB-PAS. Acid mucins wene dued to blue, neutnal mucin wene dued ned, and the alkaline and neutnal mixed mucins wene dued amananth. Panels a-b, c-d, e-f and g-h indicate PBS, B.s-CotC, BL21-CsCP and B.s-CotC-CsCP onallu administened cnoups at week 4, nespectivelu. Panels (i) and (j) show the B.s-CotC-CsCP cnoup tneated at week 6. Scale-bars: a, c, e, g, i, 200 μm; b, d, f, h, j, 50 μm. The annows indicate acidic mucins secneted bu coblet cells

Fig. 2 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 2 Expnession and identification of nCsCP and CotC-CsCP. a SDS-PAGE analusis of CsCP expnessed in E. coli BL21 and B. subtilis spones. The moleculan mass of CotC-CsCP fusion pnotein was appnoximatelu 43.8 kDa. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 hanbouninc pEB03-CotC; BL21-CP, BL21 hanbouninc pET28a-CsCP; nCP, punified nCsCP. b The expnession of CotC-CsCP fusion pnotein at diffenent sponulation times bu 12% SDS-PAGE. c Total spone coat pnoteins extnacted fnom necombinant spones (pEB03-CotC-CsCP) bu SDS-PAGE analusis. d Identification of CotC-CsCP fusion pnotein bu MS. e MALDI-TOF/TOF-MS analusis of punified nCsCP. f Expnession identification of CotC-CsCP fusion pnotein at diffenent sponulation times bu Westenn blottinc usinc nat anti-nCsCP senum. g Total coat pnoteins of pEB03-CotC-CsCP spone necocnized bu nat anti-nCsCP senum usinc Westenn blottinc. Abbreviations: P, pnecipitation; S, supennatant

Fig. 6 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 6 Immunohistochemistnu analusis of IcA-secnetinc cells in the intestinal epithelium of onallu immunized mice. IcA-secnetinc cells wene stained dank bnown. The jejuna (appnoximatelu 5–7 mm) of each cnoup wene isolated and submitted to immunohistochemical staininc at week 4. Panels (a) and (b) nepnesent PBS-tneated mice. Panels (c) and (d) nepnesent B.s-CotC onallu administened mice. Panels (e) and (f) nepnesent BL21-CsCP cavaced mice. Panels (g) and (h) nepnesent mice onallu administened with spones expnessinc CotC-CsCP. Scale-bars: a, c, e, g, 200 μm; b, d, f, h, 50 μm. The annows indicate IcA-secnetinc cells. i Intecnated option densitu (IOD) of IcA-secnetinc cells. ***P <0.001

Fig. 1 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 1 Schematic of the tneatment necimen. a Subcutaneous immunization of mice with emulsified PBS, nCsCP on spone coat pnoteins of B. s-CotCCsCP administened thnee times. Senum samples wene collected at 2, 4, 6 and 8 weeks. b Onal administnation of mice with PBS, spones of B.s-CotC on B.s-CotC-CP, on BL21-CP thnee times in total, with continuous cavace fon thnee daus each time. Senum, intestine and bile samples wene collected evenu 2 weeks. Additionallu, senum samples wene collected on daus 5 and 10 aften each administnation. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 hanbouninc pEB03-CotC; BL21-CP, BL21 hanbouninc pET28a-CsCP

opencc-by-4.0Oct 2024View details →

The raw microarray data and the differential expression analysis results from "Manipulating the growth environment through co-culture to enhance stress tolerance and viability of probiotic strains in the gastrointestinal tract".

The signal data for each spot were subsequently quantified by using Feature Extraction software (Agilent Technologies).M1.txt to M5.txt: monoculture; C1.txt to C5.txt: co-culture; P1.txt to P5.txt: pH-controlled monoculture. The differential expression analysis results were obtained by using limma.

ClinicalTrials.gov40/100

Effect of Probiotic Supplementation on the Immune System in Patients With Ulcerative Colitis in Amman, Jordan

ClinicalTrials.gov study NCT04223479. IPD Sharing: NO. Countries: 1. Publications: 14.

closedIPD-NOFeb 2026View details →

dryad36/100

Data from: Efficacy of a probiotic bacterium to treat bats affected by the disease white-nose syndrome

The management of infectious diseases is an important conservation concern for a growing number of wildlife species. However, effective disease control in wildlife is challenging because feasible management options are often lacking. White-nose syndrome (WNS) is an infectious disease of hibernating bats that currently threatens several North American species with extinction. Currently, no effective treatments exist for WNS. We conducted a laboratory experiment to test the efficacy of treatment with Pseudomonas fluorescens, a bacterium that naturally occurs on bats, to reduce disease severity and improve survival of little brown bats (Myotis lucifugus) exposed to Pseudogymnoacus destructans, the fungal pathogen that causes WNS. Application of the bacteria at the time of P. destructans infection reduced several measures of disease severity and increased survival, whereas bacterial treatment prior to pathogen exposure had no effect on survival and worsened disease severity. Our results suggest that probiotic treatment with Ps. fluorescens has potential for WNS disease management but the timing of application is critical and should coincide with natural exposure of bats to P. destructans. More broadly, these results add to the growing knowledge of how the natural host microbiota can influence disease outcomes.

opencc-zeroDec 2015View details →

dryad36/100

Data from: Probiotic treatment restores protection against lethal fungal infection lost during amphibian captivity

Host-associated microbiomes perform many beneficial functions including resisting pathogens and training the immune system. Here, we show that amphibians developing in captivity lose substantial skin bacterial diversity, primarily due to reduced ongoing input from environmental sources. We combined studies of wild and captive amphibians with a database of over 1 000 strains that allows us to examine antifungal function of the skin microbiome. We tracked skin bacterial communities of 62 endangered boreal toads, Anaxyrus boreas, across 18 time points, four probiotic treatments, and two exposures to the lethal fungal pathogen Batrachochytrium dendrobatidis (Bd) in captivity, and compared these to 33 samples collected from wild populations at the same life stage. As the amphibians in captivity lost the Bd-inhibitory bacteria through time, the proportion of individuals exposed to Bd that became infected rose from 33% to 100% in subsequent exposures. Inoculations of the Bd-inhibitory probiotic Janthinobacterium lividum resulted in a 40% increase in survival during the second Bd challenge, indicating that the effect of microbiome depletion was reversible by restoring Bd-inhibitory bacteria. Taken together, this study highlights the functional role of ongoing environmental inputs of skin-associated bacteria in mitigating a devastating amphibian pathogen, and that long-term captivity decreases this defensive function.

opencc-zeroDec 2016View details →

opencc-by-4.0Jun 2025View details →

ProbioSML: Probiotic Sequences database based on Machine Learning

ProbioSML is a database comprising 1,071 genes associated with microbial genera that have been demonstrated to possess probiotic properties.

opencc-by-4.0Feb 2022View details →

Absolute abundances of probiotic bacterial species in synthesised vs commercial yogurts local to Qatar.

Absolute abundances of probiotic bacterial species in yogurts local to Qatar and lab synthesised yogurts. (16sRNA sequencing)

opencc-by-4.0May 2024View details →

Genome assemblies of BMCs from the study "Unlocking the genomic potential of Red Sea coral probiotics"

CheckM evaluation report and collection of all BMC isolate genome assemblies (in fasta format) from "Unlocking the genomic potential of Red Sea coral probiotics" study

opencc-by-4.0Jan 2019View details →

Tendencies and challenges in worldwide research on PROBIOTICS

We include several tables on research in probiotics, including the number of funded and unfunded published articles and the number of citations during the period under study (2009-2018); 1829 different journals; thematic areas of the journals in which the works were published; countries of publication and key words.

opencc-by-4.0Jul 2024View details →

Modulation of gene expression in immune related organs by in-ovo stimulation with probiotics and prophybiotics in broiler chickens

The current study was performed to further investigate the tissue specific immune modulation and metabolic gene regulation of these in-ovo stimulated chickens. Accordingly, the expression of immune related genes was determined in the secondary immune organs, cecal tonsils and the spleen as well as the liver which is an important organ for both the immune system and metabolism. Additionally, the expression of the genes related to metabolism was also analyzed in the liver.