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Fig. 3 in Tools and Methods to Assess Field Performance Locomotion activity meter for quality assessment of mass-reared sterile male moths (Lepidoptera)

Fig. 3. Mean activity counts for before and afer pheromone exposure (lef), and the mean afer/before activity ratio (right), for un-irradiated (0 Gy = −) and irradiated (300 Gy = +) Epiphyas postvittana males. Error bars are 95% confidence limits for each mean. An afer/before ratio of 1 (marked) indicates an equal level of activity before and afer pheromone exposure.

Fig. 2 in Tools and Methods to Assess Field Performance Locomotion activity meter for quality assessment of mass-reared sterile male moths (Lepidoptera)

Fig. 2. Measured activity of male Epiphyas postvittana as summed counts at 30 s intervals in a locomotor activity monitor, before and afer pheromone stimulus of irradiated and non-irradiated moths.

Fig. 1 in Tools and Methods to Assess Field Performance Locomotion activity meter for quality assessment of mass-reared sterile male moths (Lepidoptera)

Fig. 1. An illustration of the modified Locomotion Activity Monitor set-up used for the bioassays in this study. Synthetic pheromone was puffed (2 s) from upwind to stimulate LBAM males into wing fanning. Each time a male moved through the beam array it was counted as 1 observation. Comparisons were made between pre-pheromone exposure activity and post-pheromone exposure activity for each treatment.

Fig. 5. A in Quality Control Aspects in Relation to Rearing of Moths New rearing method and larval diet for the mahogany shoot borer Hypsipyla grandella (Lepidoptera: Pyralidae)

Fig. 5. A) Average (± SE) weight of 3rd instar larvae, and B) percent survival of larvae reared on fresh cedar leaves or on artificial diet either in well plates or in Petri dishes.

Fig. 4. A in Quality Control Aspects in Relation to Rearing of Moths New rearing method and larval diet for the mahogany shoot borer Hypsipyla grandella (Lepidoptera: Pyralidae)

Fig. 4. A) Average (± SE) 3rd instar larval weight, and B) percent survival of larvae reared on fresh cedar leaves or on artificial diet in well plates.

Fig. 2. A in Quality Control Aspects in Relation to Rearing of Moths New rearing method and larval diet for the mahogany shoot borer Hypsipyla grandella (Lepidoptera: Pyralidae)

Fig. 2. A) Average (± SE) pupal weight (g), and B) average (± SE) number of d to pupation and emergence of insects reared on original or modified diets.

Fig. 4 in Tools and Methods to Assess Field Performance Locomotion activity meter for quality assessment of mass-reared sterile male moths (Lepidoptera)

Fig. 4. Mean activity counts for before and afer pheromone exposure (lef), and the mean afer/before activity ratio (right), un-irradiated (0 Gy) and irradiated (300 Gy) Epiphyas postvittana males exposed to 1 of 4 levels of temperature shock (0, 1, 2, 4 h at 30 °C). Error bars are 95% confidence limits for each mean. An afer/before ratio of 1 (marked) indicates an equal level of activity before and afer pheromone exposure.

Fig. 3 in Quality Control Aspects in Relation to Rearing of Moths New rearing method and larval diet for the mahogany shoot borer Hypsipyla grandella (Lepidoptera: Pyralidae)

Fig. 3. Average (± SE) numbers of fertile eggs, infertile eggs and hatched larvae of insects reared either on the original diet or on the new modified diet.

opencc-by-4.0Jun 2022View details →

Fig. 2 in A sustainable mass rearing method for western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae)

Fig. 2. Diagram of life cycle, rearing procedures, and label coding for Frankliniella occidentalis. (A) Frankliniella occidentalis male (lef) and female (right) adults; (B) egg-infested cotyledon; (C) nymph-infested cotyledon; (D) pupae under filter paper in rearing container; cots = cotyledons.

opencc-by-4.0Jun 2022View details →

Fig. 1 in A sustainable mass rearing method for western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae)

Fig. 1. (A) Shirofumi soybean sprout with cotyledons ready to harvest (circled). (B) Metal tray housing immature stages of Frankliniella occidentalis in insect rearing containers with secondary standard Petri dish lids on top of ventilated mesh lids. Records of dates maintained and life stages present in containers are kept on each colony lid.

opencc-by-4.0Mar 2018View details →

Spittle Island - Aphrophoridae rearing method on floating platforms

Method of laboratory rearing of field collected Aphrophoridae by confinement of juveniles-bearing plants in ventilated tubes. The tubes are placed on floating platforms that allow continuous water supply to the plants.

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Dataset: An improved rearing method and artificial diet for greyback canegrubs (Dermolepida albohirtum, Coleoptera: Scarabaeidae).

This dataset was generated and analysed for a study aimed at developing a rearing method and artificial diet for greyback canegrubs (Dermolepida albohirtum, Coleoptera: Scarabaeidae). See the published study for more details on the methodology. Egg monitoring Excel spreadsheet. Information about Dermolepida albohirtum eggs and hatching rate. Eggs were laid by adult beetles in the laboratory. Eggs were kept in hatching chambers in small batches and stored in an incubator at 26°C, 75% humidity, and in the dark. Eggs hatched over several days (up to five days). Hatchling monitoring Excel spreadsheet. Information about Dermolepida albohirtum hatchlings and the date at which they hatched and developed into second instar in the laboratory. Hatchlings were fed pieces of carrots. Survival grubs on diet Excel spreadsheet. Information about Dermolepida albohirtum second instar larvae fed different diets or not fed in the laboratory for 18 days: survival, date at which they developed into third instar, date at which they died (if applicable). Weight of larvae on diet Excel spreadsheet. Weight of Dermolepida albohirtum larvae fed different diets or not fed in the laboratory for 18 days. Width of larvae on diet Excel spreadsheet. Width of Dermolepida albohirtum larvae fed different diets or not fed in the laboratory for 18 days. Larval width was measured as a straight line across the maximal width situated between the raster and the last spiracle. The measurements were taken from photographs and using ImageJ software (version 1.52a). Activity level of larvae on diet Excel spreadsheet. Activity level of Dermolepida albohirtum larvae fed different diets or not fed in the laboratory for 18 days. Larvae were observed at 30 seconds intervals for 10 minutes every 3 days (i.e., when new diet was added). We recorded when larvae started digging into the sand and when they were successfully buried at 30 seconds intervals for 10 minutes to determine their relative activity level. We also recorded the percentage of their body outside the substrate if they were not completely buried after 10 minutes. Diet uptake Excel spreadsheet. Diet uptake of Dermolepida albohirtum larvae fed different diets in the laboratory for 18 days. We calculated the amount of food source (diet PL1 or carrot) ingested by larvae by subtracting the initial diet weight with the weight of the remaining diet after three days (i.e., when new diet was added). These values were corrected by adding or subtracting the average proportion of diet or carrot weight change measured in controlled containers (i.e., carrots gained 11.2% of their weight on average and diet PL1 lost 1.9% of their weight in controlled conditions).

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Fig. 1 in Quality Control Aspects in Relation to Rearing of Moths New rearing method and larval diet for the mahogany shoot borer Hypsipyla grandella (Lepidoptera: Pyralidae)

Fig. 1. Oviposition cage. The fan provides airflow through the cage.

opencc-by-4.0Jun 2015View details →

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Figures 1–2. Rearing method. 1 in Eucnemid larvae of the Nearctic region. Part V: Fifth instar larval descriptions for eight species of Microrhagus Dejean, 1833 (Coleoptera: Eucnemidae: Melasinae: Dirhagini), with descriptions of four new species and notes on their biology

Figures 1–2. Rearing method. 1) Storage bag with rotten wood and data. 2) Examined rotten wood.

opennotspecifiedMar 2021View details →

Fig. 2 in Biology of the Small Diving Beetle Laccophilus yoshitomii Watanabe and Kamite, 2018 (Coleoptera: Dytiscidae) and Rearing Methods

Fig. 2. Histogram of the number of days in each developmental stage, from first instar larvae to escaping to the soil surface after emergence.

opennotspecifiedFeb 2016View details →

FIGURE 1. Neighbour joining COI gene tree using uncorrected p in Description of the final instar larva of Orthetrum borneense Kimmins, 1936 (Odonata, Libellulidae), using rearing and molecular methods

FIGURE 1. Neighbour joining COI gene tree using uncorrected p–distance for adults of nine species and subspecies of Orthetrum plus two outgroup taXa, and a larva of O. borneense.

opennotspecifiedFeb 2016View details →

FIGURE 4 in Description of the final instar larva of Orthetrum borneense Kimmins, 1936 (Odonata, Libellulidae), using rearing and molecular methods

FIGURE 4. Orthetrum borneense ♂ larva detail drawings: (a) prementum dorsal (b) labial palp frontal (c) anal pyramid dorsal (d) anal pyramid lateral (e) abdomen (S4–10) lateral (f) wing cases lateral; scale bars 1 mm.

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Data from: Rearing and sampling methods for estimating spruce budworm development rates at constant temperatures

We describe an experimental protocol for measuring the response of spruce budworm post-diapause larval development to temperature. This protocol is specifically designed to include measurements of development near their upper and lower thermal thresholds. The application of this protocol to a laboratory colony allowed for the first experimental evidence that spruce budworm larval development occurs at temperatures as low as 5 ºC and as high as 35 ºC and provides data to estimate development rates at temperatures from 5–35 ºC in 5 ºC increments. Our protocol is also designed to minimize mortality near the thermal development thresholds thus allowing for multi-generational studies. We observed developmental plasticity in larvae reared at constant temperatures, particularly the occurrence of up to 42% of some individuals requiring only five instars to complete development, compared to the expected six instars. An occurrence that exhibited no clear relation to temperature. While this protocol is specifically designed for spruce budworm, it provides a template for the study of other species' developmental responses to temperature.

opencc-zeroJul 2021View details →