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Dataset results
14 results for “root apex”
Comparative gene expression analysis in the Arabidopsis thaliana root apex using RNA-seq and microarray transcriptome profiles
The root apex is an important section of the plant root involved in environmental sensing and cellular development. Analyzing the gene profile of root apex in diverse environments is important and challenging especially when the samples are limiting and precious such as in spaceflight. The feasibility of using tiny root sections for transcriptome analysis was examined in this study. To understand the gene expression profiles of the root apex Arabidopsis thaliana Col-0 roots were sectioned into Zone-I (0.5 mm root cap and meristematic zone) and Zone-II (1.5 mm transition elongation and growth terminating zone). Gene expression was analyzed using microarray and RNA seq. Both the techniques arrays and RNA-Seq identified 4180 common genes as differentially expressed (with > two-fold changes) between the zones. In addition 771 unique genes and 19 novel TARs were identified by RNA-Seq as differentially expressed which were not detected in the arrays. Single root tip zones can be used for full transcriptome analysis; further the root apex zones are functionally very distinct from each other. RNA-Seq provided novel information about the transcripts compared to the arrays. These data will help optimize transcriptome techniques for dealing with small rare samples.
Transcriptomic analysis reveals the early signaling events of narciclasine in Arabidopsis root apex
GEO Series GSE80815. Arabidopsis thaliana. 4 samples. Type: Expression profiling by high throughput sequencing.
Nicotinana tabacum cv. K326 long day diurnal timecourse: Root, Shoot, Shoot Apex
GEO Series GSE95717. Nicotiana tabacum. 36 samples. Type: Expression profiling by high throughput sequencing.
Accuracy of Electronic Apex Locators ProMark, RootZX II and NRG Rider on Working Length of Multi-rooted Teeth
ClinicalTrials.gov study NCT02521909. IPD Sharing: NO. Countries: 1. Publications: 0.
Comparative gene expression analysis in the Arabidopsis thaliana root apex using RNA-seq and microarray transcriptome profiles [RNA-seq]
GEO Series GSE115554. Arabidopsis thaliana. 6 samples. Type: Expression profiling by high throughput sequencing.
Assessment Root Apex Position of Mandibular Molars and Premolars Linear to Inferior Alveolar Canal Among Egyptian Population Using CBCT
ClinicalTrials.gov study NCT03537209. IPD Sharing: NO. Countries: 0. Publications: 0.
Effect of Different Apex Locators on Postoperative Pain in Endodontic Retreatment of Single-Rooted Teeth: Prospective Randomized Clinical Study
ClinicalTrials.gov study NCT06964490. IPD Sharing: NO. Countries: 0. Publications: 0.
Transcript profiling of auxin/cytokinin crosstalk in the Arabidopsis primary root apex
GEO Series GSE20231. Arabidopsis thaliana. 14 samples. Type: Expression profiling by array.
SOL2 mutation affect gene expresstion at root apex
GEO Series GSE11119. Arabidopsis thaliana. 4 samples. Type: Expression profiling by array.
Comparative gene expression analysis in the Arabidopsis thaliana root apex using RNA-seq and microarray transcriptome profiles [microarray]
GEO Series GSE115486. Arabidopsis thaliana. 6 samples. Type: Expression profiling by array.
Comparing RNA-Seq and microarray gene expression data in two zones of the Arabidopsis root apex relevant to spaceflight
GEO Series GSE115555. Arabidopsis thaliana. 12 samples. Type: Expression profiling by array; Expression profiling by high throughput sequencing.
Comparing RNA-Seq and microarray gene expression data in two zones of the Arabidopsis root apex relevant to spaceflight.
Premise of the study: The root apex is an important region involved in environmental sensing, but comprises a very small part of the root. Obtaining root apex transcriptomes is therefore challenging when the samples are limited. The feasibility of using tiny root sections for transcriptome analysis was examined, comparing RNA sequencing (RNA-Seq) to microarrays in characterizing genes that are relevant to spaceflight.Methods:Arabidopsis thaliana Columbia ecotype (Col-0) roots were sectioned into Zone 1 (0.5 mm; root cap and meristematic zone) and Zone 2 (1.5 mm; transition, elongation, and growth-terminating zone). Differential gene expression in each was compared.Results: Both microarrays and RNA-Seq proved applicable to the small samples. A total of 4180 genes were differentially expressed (with fold changes of 2 or greater) between Zone 1 and Zone 2. In addition, 771 unique genes and 19 novel transcriptionally active regions were identified by RNA-Seq that were not detected in microarrays. However, microarrays detected spaceflight-relevant genes that were missed in RNA-Seq. Discussion: Single root tip subsections can be used for transcriptome analysis using either RNA-Seq or microarrays. Both RNA-Seq and microarrays provided novel information. These data suggest that techniques for dealing with small, rare samples from spaceflight can be further enhanced, and that RNA-Seq may miss some spaceflight-relevant changes in gene expression.
Glycome profiling and immunohistochemistry uncover changes in cell walls of Arabidopsis thaliana roots during spaceflight: Advanced Plant EXperiment (APEX) 03-1 Raw Dataset
This study was conducted to uncover the underlying molecular mechanisms by which microgravity impacts cell wall architecture in the model plant Arabidopsis thaliana using the Vegetable Production System (Veggie) housed on the International Space Station (ISS). Based on our previous space flight studies using the Biological Research in Canisters (BRIC) hardware on the STS-131 Space Shuttle mission, we found that plants respond to microgravity in large part through the transcriptional reprogramming of genes that control cell wall remodeling. This follow-up spaceflight experiment called the Advanced Plant EXperiments (APEX 03-1) in Space was designed to use Veggie unit on the ISS and on the ground unit on the Space Station Processing Facility (SSPF) to probe deeper into spaceflight-induced cell wall associated transcript expression changes. In this study we used RNA-Seq in combination with glycome profiling (glycomics) to correlate spaceflight-induced gene expression changes with actual cell wall modifications within seedlings.
The spatial differences of root apex contribute to improved drought tolerance as induced by drought priming in wheat
GEO Series GSE133921. Triticum aestivum. 36 samples. Type: Expression profiling by high throughput sequencing.
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