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384 results for “scaling analysis”
Annual precipitation and photo-derived vegetation and litter cover (2013-2021) used for analysis in the manuscript “Growing grasses in the desert: Multi-scale Interactions and State Change Reversal in Drylands”
This dataset contains water year precipitation collected from meteorological stations, litter and vegetation cover values derived from overhead photos, and litter and soil accumulation in lateral photos in a long-term experiment (2013-2021) of cross-scale interactions (CSIs) at the Jornada Basin LTER site in southern New Mexico, U.S.A. Manipulations were initiated in 2013 at 15 experimental blocks, each with 4 treatment plots: plant-scale herbicide of mesquite shrubs, patch-scale connectivity modifiers (ConMods), herbicide + ConMods, control without manipulations. Litter, soil, and vegetation cover were estimated using repeat overhead photographs of microplots within treatment and control plots. Litter and soil accumulation were estimated from lateral photos of ConMods. This dataset utilized QuickBird imagery from 2011 to assess ground cover classes within the Jornada Basin, focusing on bare ground, herbaceous, and shrub cover. Daily precipitation data collected from 13 meteorological stations were used to calculate water year (1 October-30 September) precipitation from 2013 through 2021. This dataset provides supporting data for the manuscript "Growing grasses in the desert: Multi-scale Interactions and State Change Reversal in Drylands" by Peters et al.
Marine plastics alter the organic matter composition of the air-sea boundary layer, with influences on CO2 exchange: a large-scale analysis method to explore future ocean scenarios
<p>Microplastics are substrates for microbial activity and can influence biomass production. This has potentially important implications in the sea-surface microlayer, the marine boundary layer that controls gas exchange with the atmosphere and where biologically produced organic compounds can accumulate. In the present study, we used six large scale mesocosms to simulate future ocean scenarios of high plastic concentration. Each mesocosm was filled with 3 m3 of seawater from the oligotrophic Sea of Crete, in the Eastern Mediterranean Sea. A known amount of standard polystyrene microbeads of 30 μm diameter was added to three replicate mesocosms, while maintaining the remaining three as plastic-free controls. Over the course of a 12-day experiment, we explored microbial organic matter dynamics in the sea-surface microlayer in the presence and absence of microplastic contamination of the underlying water. Our study shows that microplastics increased both biomass production and enrichment of carbohydrate-like and proteinaceous marine gel compounds in the sea-surface microlayer. Importantly, this resulted in a 3 % reduction in the concentration of dissolved CO2 in the underlying water. This reduction was associated to both direct and indirect impacts of microplastic pollution on the uptake of CO2 within the marine carbon cycle, by modifying the biogenic composition of the sea's boundary layer with the atmosphere.</p>
ExcapeDB: An integrated large scale dataset facilitating Big Data analysis in chemogenomics
<p>ExcapeDB: An integrated large scale dataset facilitating Big Data analysis in chemogenomics</p> <p>Supplementary file (full dataset download)</p> <p>- v2 with SMILES errors fixed (19.01.2019)</p>
CROSS-VALIDATION OF FUNCTIONAL MRI and PARANOID-DEPRESSIVE SCALE: BRAIN SIGNATURES FROM MULTIVARIATE ANALYSIS
<p>Brain signatures identified by bottom-up unsupervised machine learning: three principal components based on activations yielded from the three kinds of diagnostically relevant stimuli are used in order to produce cross-validation markers which may effectively predict the variance on the level of clinical populations and eventually delineate diagnostic and classification groups. The stimuli represent items from a paranoid-depressive self-evaluation scale, administered simultaneously with functional magnetic resonance imaging (fMRI).</p> <p>We have been able to separate the two investigated clinical entities – schizophrenia and recurrent depression by use of multivariate linear model and principal component analysis. This is a confirmation of the possibility to achieve bottom-up classification of mental disorders, by use of the brain signatures relevant to clinical evaluation tests.</p>
EMBERSim: A Large-Scale Databank for Boosting Similarity Search in Malware Analysis
<p>In recent years there has been a shift from heuristics-based malware detection towards machine learning, which proves to be more robust in the current heavily adversarial threat landscape. While we acknowledge machine learning to be better equipped to mine for patterns in the increasingly high amounts of similar-looking files, we also note a remarkable scarcity of the data available for similarity-targeted research. Moreover, we observe that the focus in the few related works falls on quantifying similarity in malware, often overlooking the clean data. This one-sided quantification is especially dangerous in the context of detection bypass. We propose to address the deficiencies in the space of similarity research on binary files, starting from EMBER — one of the largest malware classification data sets. We enhance EMBER with similarity information as well as malware class tags, to enable further research in the similarity space. Our contribution is threefold: (1) we publish EMBERSim, an augmented version of EMBER, that includes similarity-informed tags; (2) we enrich EMBERSim with automatically determined malware class tags using the open-source tool AVClass on VirusTotal data and (3) we describe and share the implementation for our class scoring technique and leaf similarity method.</p>
Video Analysis Scale of Engagement (VASE): Initial and Final Protocols
<p>These files contain the initial and final protocols that were used to develop the Video Analysis Scale for Engagement (VASE). The abstract of our article in Wellcome Open Research, 5:230: https://wellcomeopenresearch.org/articles/5-230/v2:</p> <p><strong>Background</strong>: The current study sought to develop a valid, reliable and unobtrusive tablet computer-based observational measure to assess engagement of people with advanced dementia. The Video Analysis Scale of Engagement (VASE) was designed to enable the rating of moment-by-moment changes in engagement during an activity, which would be useful for both future research and current residential care. <strong>Methods:</strong> An initial version of the VASE was tested. Face validity and content validity were assessed to validate an operational definition of engagement and develop an acceptable protocol for the scale. Thirty-seven non-professional and professional volunteers were recruited to view and rate level of engagement in music activities using the VASE. <strong>Results</strong>: An inter-class coefficient (ICC) test gave a high level of rating agreement across professionals and non-professionals. However, the ICC results of within-professionals were mixed. Linear mixed modelling suggested that the types of interventions (active or passive music listening), the particular intervention session being rated, time period of video and the age of raters could affect the ratings. <strong>Conclusions</strong>: Results suggested that raters used the VASE in a dynamic fashion and that the measure was able to distinguish between interventions. Further investigation and adjustments are warranted for this to be considered a valid and reliable scale in the measurement of engagement of people with advanced dementia in a residential care setting.</p>
Efficient and accurate framework for genome-wide gene-environment interaction analysis in large-scale biobanks
<p>Gene-environment interaction (GxE) analysis elucidates the interplay between genetic predispositions and environmental influences, offering significant potential for precision medicine. With the increasing use of electronic health records (EHR) linked to genetic data in large-scale biobanks, genome-wide association studies (GWAS) have expanded to encompass complex traits with intricate structures, such as time-to-event and ordinal categorical traits. Although these complex traits convey more phenotypic information, most existing scalable genome-wide GxE analysis approaches only focus on quantitative or binary traits. In this work, we propose a scalable and accurate analysis framework, SPAGxE<sub>CCT</sub>, that is applicable to a wide variety of trait types. We extend SPAGxE to SPAGxE+, which can account for sample relatedness. In addition, we extend SPAGxE<sub>CCT</sub> to SPAGxEmix<sub>CCT</sub>, which accounts for population stratification and is applicable to include individuals from multiple ancestries or admixed populations. We applied SPAGxE<sub>CCT</sub>, SPAGxE+, and SPAGxEmix<sub>CCT</sub> to analyze time-to-event traits in UK Biobank. For the SPAGxE<sub>CCT</sub> analyses, 281,149 White British individuals were included. For the SPAGxE+ analyses, 337,367 WB individuals with sample relatedness were included. For the SPAGxEmix<sub>CCT</sub> analyses, 338,044 individuals from all ancestries were included. SPAGxE<sub>CCT</sub>, SPAGxE+, and SPAGxEmix<sub>CCT</sub> are computationally efficient to analyze large datasets with hundreds of thousands of individuals, can accurately control type I error rates while remaining powerful to identify novel GxE findings.</p>
GIS Protocol for Multy-Scale Emerging Hot Spot Analysis
<p>This GIS protocol is primarily intended as supplementary material to the article (Štular et al., 2022). The article contains important contextual information about its intended use. In short, this GIS protocol was developed for the purposes of archaeological regional analysis of spatial data. The data are provided elsewhere in spreadsheet format (Štular et al., 2021). Data in GIS format are included in this repository. The GIS protocol can be used with any relevant data for any purpose as long as the data format matches the format of the included data.</p> <p>Includes GIS protocol (textual description) and GIS data in *.shp format.</p>
The COUGHVID crowdsourcing dataset: A corpus for the study of large-scale cough analysis algorithms
<p><strong>Overview</strong></p> <p>Cough audio signal classification has been successfully used to diagnose a variety of respiratory conditions, and there has been significant interest in leveraging Machine Learning (ML) to provide widespread COVID-19 screening. The COUGHVID dataset provides over 30,000 crowdsourced cough recordings representing a wide range of subject ages, genders, geographic locations, and COVID-19 statuses. Furthermore, experienced pulmonologists labeled more than 2,000 recordings to diagnose medical abnormalities present in the coughs, thereby contributing one of the largest expert-labeled cough datasets in existence that can be used for a plethora of cough audio classification tasks. As a result, the COUGHVID dataset contributes a wealth of cough recordings for training ML models to address the world’s most urgent health crises.</p> <p><strong>Private Set and Testing Protocol</strong></p> <p>Researchers interested in testing their models on the private test dataset should contact us at coughvid@epfl.ch, briefly explaining the type of validation they wish to make, and their obtained results obtained through cross-validation with the public data. Then, access to the unlabeled recordings will be provided, and the researchers should send the predictions of their models on these recordings. Finally, the performance metrics of the predictions will be sent to the researchers. The private testing data is not included in any file within our Zenodo record, and it can only be accessed by contacting the COUGHVID team at the aforementioned e-mail address.</p> <p><strong>New Semi-Supervised Labeling</strong></p> <p>The third version of the COUGHVID dataset contains thousands of additional recordings obtained through October 2021. Additionally, the recordings containing coughs were re-labeled according to a semi-supervised learning algorithm that combined the user labels with those of the expert physicians, which were modeled using ML and expanded on the previously unlabeled data. These labels can be found in the "status_SSL" column of the "metadata_compiled.csv" file.</p>
Data for Rasch analysis of the three Field Practice Experiences Scales
<p>Data for Rasch analysis of the three Field Practice Experiences Scales. Data rare from Danish teacher education program students. Contains the following variables:</p> <p>Variables O1 to O12 are the items for the Observed scale</p> <p>Variables P1 to P12 are the items for the Practised scale</p> <p>Variables F1 to F12 are the items to the Received feedback scale</p> <p>Gender: 1 = female, 2 = male</p> <p>Campus: 1 = campus A, 2 = campus B</p> <p>T_Progr (teacher education program): 1 = regular, 2 = other</p> <p>P_level (level of latest field practice placement): 1 = 3rd level, 2 = 2nd level, 3 = 1st level</p> <p>Age: 1 = 24 years and younger, 2 = 25 years and older</p>
Human pancreatic islet microRNAs implicated in diabetes and related traits by large-scale genetic analysis
<p>Genetic studies have identified ≥240 loci associated with risk of type 2 diabetes (T2D), yet most of these loci lie in non-coding regions, masking the underlying molecular mechanisms. Recent studies investigating mRNA expression in human pancreatic islets have yielded important insights into the molecular drivers of normal islet function and T2D pathophysiology. However, similar studies investigating microRNA (miRNA) expression remain limited. Here, we present data from 63 individuals, the largest sequencing-based analysis of miRNA expression in human islets to date. We characterize the genetic regulation of miRNA expression by decomposing the expression of highly heritable miRNAs into <em>cis</em>- and <em>trans</em>-acting genetic components and mapping <em>cis</em>-acting loci associated with miRNA expression (miRNA-eQTLs). We find (i) 84 heritable miRNAs, primarily regulated by <em>trans</em>-acting genetic effects, and (ii) 5 miRNA-eQTLs. We also use several different strategies to identify T2D-associated miRNAs. First, we colocalize miRNA-eQTLs with genetic loci associated with T2D and multiple glycemic traits, identifying one miRNA, miR-1908, that shares genetic signals for blood glucose and glycated hemoglobin (HbA1c). Next, we intersect miRNA seed regions and predicted target sites with credible set SNPs associated with T2D and glycemic traits and find 32 miRNAs that may have altered binding and function due to disrupted seed regions. Finally, we perform differential expression analysis and identify 14 miRNAs associated with T2D status—including miR-187-3p, miR-21-5p, miR-668, and miR-199b-5p—and 4 miRNAs associated with a polygenic score for HbA1c levels—miR-216a, miR-25, miR-30a-3p, and miR-30a-5p.</p>
Supporting information for "Few-Shot Learning Enables Population-Scale Analysis of Leaf Traits in Populus trichocarpa"
<p><strong>Description</strong></p> <p>In this work, we use few-shot learning to segment the body and vein architecture of <em>P. trichocarpa</em> leaves from high-resolution scans obtained in the UC Davis common garden. Leaf and vein segmentation are formulated as separate tasks, in which convolutional neural networks (CNNs) are used to iteratively expand partial segmentations until reaching stopping criteria. Our leaf and vein segmentation approaches use just 50 and 8 manually traced images for training, respectively, and are applied to a set of 2,634 top and bottom leaf scans. We show that both methods achieve high segmentation accuracy and retain biologically realistic features. The leaf and vein segmentations are compared against a U-Net baseline model, and subsequently used to extract 68 morphological traits using traditional open-source image processing tools, which are validated using real-world physical measurements. For a biological perspective, we perform a genome-wide association study using the "vein density" trait to discover novel genetic architectures associated with multiple physiological processes relating to leaf development and function. In addition to sharing all of the few-shot learning code, we are releasing all images, manual segmentations, model predictions, 68 extracted leaf phenotypes, and a new set of SNPs called against the v4 <em>P. trichocarpa</em> genome for 1,419 genotypes.</p> <p><strong>Directories:</strong></p> <pre><code>Few-shot learning for p. trichocarpa leaf traits ├── data │ ├── genomes │ │ ├── Ptri_V4_Nisq1.[...].bed │ │ ├── Ptri_V4_Nisq1.[...].bim │ │ └── Ptri_V4_Nisq1.[...].fam │ ├── images │ │ └── *.jpeg │ ├── leaf_masks │ │ └── *.png │ ├── leaf_preds │ │ └── *.png │ ├── leaf_unet_preds │ │ └── *.png │ ├── results │ │ ├── digital_traits.tsv │ │ ├── gwas_results.csv │ │ ├── manual_traits.tsv │ │ ├── vein_density_blups.tsv │ │ └── vein_density_tps_adj.tsv │ ├── vein_bce_preds │ │ └── *.png │ ├── vein_bce_probs │ │ └── *.png │ ├── vein_fl_preds │ │ └── *.png │ ├── vein_fl_probs │ │ └── *.png │ ├── vein_masks │ │ └── *.png │ ├── vein_unet_bce_preds │ │ └── *.png │ ├── vein_unet_bce_probs │ │ └── *.png │ ├── vein_unet_fl_preds │ │ └── *.png │ ├── vein_unet_fl_probs │ │ └── *.png ├── figures │ └── *.png ├── logs │ ├── leaf_tracer_256.txt │ ├── leaf_unet_256.txt │ ├── vein_grower_bce_128.txt │ ├── vein_grower_fl_128.txt │ ├── vein_unet_bce_128.txt │ └── vein_unet_fl_128.txt ├── models │ ├── BuildCNN.py │ ├── BuildUNet.py │ ├── LeafTracer.py │ └── VeinGrower.py ├── notebooks │ ├── Figures.ipynb │ ├── GrowerInference.ipynb │ ├── GrowerTraining.ipynb │ ├── TracerInference.ipynb │ ├── TracerTraining.ipynb │ ├── UNetLeafSegmentation.ipynb │ └── UNetVeinSegmentation.ipynb ├── utils │ ├── GetLowestGPU.py │ ├── ImageLoader.py │ ├── LeafGenerator.py │ ├── ModelWrapperGenerator.py │ ├── TimeRemaining.py │ ├── TraceInitializer.py │ ├── UNetTileGenerator.py │ └── VeinGenerator.py └── weights ├── leaf_tracer_256_best_val_model.save ├── leaf_unet_256_best_val_model.save ├── vein_grower_bce_128_best_val_model.save ├── vein_grower_fl_128_best_val_model.save ├── vein_unet_bce_128_best_val_model.save └── vein_unet_fl_128_best_val_model.save </code></pre> <p><strong>Data:</strong></p> <p>The <code>data</code> folder includes all images, ground truth segmentations, predicted segmentations, and extracted leaf traits. All images encode the sample ID in the file name by indicating the treatment, block, row, position, and leaf side, respectively. For example, the file, <code>C_1_1_2_bot.jpeg</code>, indicates the control treatment, block 1, row 1, position 2, and the bottom side of the leaf. Tabulated results include position IDs as well as the corresponding genotype IDs.</p> <ul> <li>The <code>images</code> folder includes the 2,906 high-resolution leaf scans taken in the field.</li> <li>The <code>leaf_masks</code> folder includes 50 ground truth segmentations used for training the leaf tracing algorithm.</li> <li>The <code>leaf_preds</code> folder includes the 2,906 predicted segmentations from the leaf tracing algorithm.</li> <li>The <code>leaf_unet_preds</code> folder includes the 2,906 predicted segmentations from the U-Net model for leaf segmentation.</li> <li>The <code>vein_masks</code> folder includes 8 ground truth segmentations used for training the vein growing algorithm.</li> <li>The <code>vein_*_preds</code> folder includes the 1,453 predicted segmentations from the vein growing algorithm, where * specifies the loss function (bce: binary cross-entropy, fl: focal loss).</li> <li>The <code>vein_*_probs</code> folder includes the 1,453 predicted probability maps from the vein growing algorithm before thresholding, where * specifies the loss function (bce: binary cross-entropy, fl: focal loss).</li> <li>The <code>vein_unet_*_preds</code> folder includes the 1,453 predicted segmentations from the U-Net model for vein segmentation, where * specifies the loss function (bce: binary cross-entropy, fl: focal loss).</li> <li>The <code>vein_unet_*_probs</code> folder includes the 1,453 predicted probability maps from the U-Net model for vein segmentation before thresholding, where * specifies the loss function (bce: binary cross-entropy, fl: focal loss).</li> <li>The <code>genomes</code> folder includes the set of SNPs called against the v4 <em>P. trichocarpa</em> genome for 1,419 genotypes with a README file detailing the steps taken.</li> <li>The <code>results</code> folder includes <ul> <li>Raw values of the 68 predicted leaf traits in <code>digital_traits.tsv</code></li> <li>Manually measured values of petiole length and width in <code>manual_traits.tsv</code></li> <li>Thin plate spline (TPS) adjusted values of the vein density trait in <code>vein_density_tps_adj.tsv</code></li> <li>Best linear unbiased prediction (BLUP) adjusted values of the vein density trait in <code>vein_density_blups.tsv</code></li> <li>GWAS results for the vein density trait, including chromosome positions and corresponding P values, in <code>gwas_results.csv</code></li> </ul> </li> </ul> <p><strong>Figures:</strong></p> <p>The <code>figures</code> folder includes all figures and videos used in the manuscript. See <code>notebooks/Figures.ipynb</code> for the methods used to generate these figures.</p> <p><strong>Logs:</strong></p> <p>The <code>logs</code> folder includes logs of CNN convergence for the training and validation sets during model training for the leaf tracing CNN vein growing CNN, and U-Net models. The file names include the model, loss function (bce: binary cross-entropy, fl: focal loss), and size of the input window for each method (e.g., 128 for the vein growing CNN).</p> <p><strong>Models:</strong></p> <p>The <code>models</code> folder includes the CNN implementations in PyTorch as well as the leaf tracing and vein growing algorithms at inference time.</p> <ul> <li><code>BuildCNN.py</code> defines the CNN architecture for leaf tracing or vein growing, with user-specified input shape, output shape, layers, and output activation functions.</li> <li><code>BuildUNet.py</code> defines the U-Net architecture for leaf and vein segmentation, with user-specified input/output shape, layers, and output activation functions.</li> <li><code>LeafTracer.py</code> defines the leaf tracing algorithm at inference time.</li> <li><code>VeinGrower.py</code> defines the vein growing algorithm at inference time.</li> </ul> <p><strong>Notebooks:</strong></p> <p>The <code>notebooks</code> folder includes Jupyter notebooks used for model training, model inference, and figure generation.</p> <ul> <li><code>Figures.ipynb</code> is used to generate all of the manuscript figures.</li> <li><code>GrowerTraining.ipynb</code> is used to train the vein growing CNN.</li> <li><code>GrowerInference.ipynb</code> is used to apply the vein growing algorithm to the 1,453 leaf bottom images.</li> <li><code>TracerTraining.ipynb</code> is used to train the leaf tracing CNN.</li> <li><code>TracerInference.ipynb</code> is used to apply the leaf tracing algorithm to the 2,906 leaf top and bottom images.</li> <li><code>UNetLeafSegmentation.ipynb</code> is used to train and apply U-Net for leaf segmentation.</li> <li><code>UNetVeinSegmentation.ipynb</code> is used to train and apply U-Net for vein segmentation.</li> </ul> <p><strong>Utils:</strong></p> <p>The <code>utils</code> folder includes utility scripts implemented in Python that assist in model training and inference.</p> <ul> <li><code>ImageLoader.py</code> loads image/mask pairs for sampling training/validation tiles.</li> <li><code>LeafGenerator.py</code> generates inputs/outputs for the leaf tracing CNN.</li> <li><code>VeinGenerator.py</code> generates inputs/outputs for the vein growing CNN.</li> <li><code>UNetTileGenerator.py</code> generates inputs/outputs for the U-Net model.</li> <li><code>GetLowestGPU.py</code> identifies available GPUs using the <code>nvidia-smi</code> command and selects the one with lowest memory usage, if none available the device is set to CPU.</li> <li><code>ModelWrapperGenerator.py</code> wraps the PyTorch CNN and data loaders with similar functionality to the Keras Model class in TensorFlow (e.g., model.fit(...)).</li> <li><code>TimeRemaining.py</code> is used by the model wrapper to estimate remaining time left per epoch.</li> <li><code>TraceInitializer.py</code> is used by the tracing algorithm at inference time to initialize the leaf trace using automatic thresholding.</li> </ul> <p><strong>Weights:</strong></p> <p>The <code>weights</code> folder includes the CNN parameters from the epoch resulting in the best validation error. The file names include the model, loss function (bce: binary cross-entropy, fl: focal loss), and size of the input window for each method (e.g., 128 for the vein growing CNN). The weights are loaded into the CNN models for inference.</p> <p><strong>Citation:</strong></p> <pre><code>@article{ doi:10.34133/plantphenomics.0072, author = {John Lagergren and Mirko Pavicic and Hari B. Chhetri and Larry M. York and Doug Hyatt and David Kainer and Erica M. Rutter and Kevin Flores and Jack Bailey-Bale and Marie Klein and Gail Taylor and Daniel Jacobson and Jared Streich }, title = {Few-Shot Learning Enables Population-Scale Analysis of Leaf Traits in Populus trichocarpa}, journal = {Plant Phenomics}, volume = {0}, number = {ja}, pages = {}, year = {}, doi = {10.34133/plantphenomics.0072}, URL = {https://spj.science.org/doi/abs/10.34133/plantphenomics.0072}, eprint = {https://spj.science.org/doi/pdf/10.34133/plantphenomics.0072}, }</code></pre>
Unraveling the secrets of plant roots: Simplified method for large scale root exudate sampling and analysis in Arabidopsis thaliana
<p>Plants exude a plethora of compounds, both above- and below ground, to communicate with their environment. Although much is known about this communication above ground, we are only beginning to fathom the identity, level of variation, and role of below-ground chemical signals in a plant’s life. There have been many challenges associated with establishing a standardized methodology for studying root-exuded compounds, thus their role in plant-environment communication is still not well described.</p> <p>Here, we develop an interdisciplinary workflow to explore the natural variation in root exudate chemical composition of the model plant <em>Arabidopsis thaliana</em>. We highlight key challenges associated with sampling strategies and develop a framework for analyzing narrow and broad-scale patterns in root exudate composition of a large sample set of natural <em>A. thaliana</em> accessions.</p> <p>Our method consists of <em>in vitro</em> cultivation of individual seedlings inside a plastic mesh followed by a short hydroponic sampling period in micro quantities of ultrapure water. The mesh eases handling individual plants of varying sizes, thus making this setup advantageous for large-scale characterization of root exudates of individual plants in axenic conditions, and can be easily extended for prolonged temporal exudate collection experiments. Furthermore, a short sampling duration minimizes the experiment duration from days to mere hours, and is validated by yielding sufficient signal even with the small volume of sampling solution. An untargeted metabolic profiling analytical approach using ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-QTOF-MS), followed by compound identification using open access software MZMine3 and SIRIUS 5, was used to capture a broad picture of the root exudate composition of <em>A. thaliana</em> accessions. This methodology can be broadly applied for investigating the role of root exudates as signals involved in plant belowground interactions. We report the first findings from the analysis here with results from Columbia genotype.</p> <p>We include here</p> <p> </p> <p>In <strong>Raw datafiles</strong>: The raw datasets obtained from MZmine 3 analysis, which contains aligned features of Columbia genotypes (Sheet1) as well as control samples (Sheet2). The dataset consists of <em>feature (row)ID, average (mass-to-charge ratios) m/z</em> and <em>retention times (RT)</em> across samples for individual features. It also includes sample-specific information including <em>feature status, name, m/z, RT, feature peak height,</em> and <em>area</em>. We also include a filtered datasheet excluding the features obtained in control as well as samples (Sheet3). Sheet 4 contains the phenotypic data on the number of leaves and rosette size of the 28 replicates at the time of sampling, along with the total peak area for each sample from MZmine data.</p> <p>In <strong>Supplementary tables</strong>: The 354 metabolites obtained after filtering out control features are listed with their <em>mass-to-charge ratios</em>, <em>retention times, and the mean, variance and coefficient of variation of the peak areas </em>(Supplementary Table 1). Supplementary Table 2 details the features identified by SIRIUS 5 with their <em>mass-to-charge ratios, retention times, chemical formula, chemical annotations,</em> and corresponding <em>probabilities scored by SIRIUS</em></p> <p>In <strong>Extended data analysis:</strong> Contains supporting data analysis for reproducibility and validity of our method for root exudate collection and analysis in <em>Arabidopsis thaliana.</em> These extended data analyses enhance the understanding of the relationship between plant phenotypic traits, peak area, and variation in compound abundance</p>
A scalable, accurate, and universal analysis framework using individual-level allele frequency for large-scale genetic association studies in an admixed population
<p>Inclusion of individuals with diverse or admixed genetic ancestries is crucial to discover novel findings that may be missed by genomics analyses rooted solely in Caucasian population. Here, we present an analysis framework, SPAmix, which is scalable to a large-scale biobank data analysis including hundreds of thousands of admixed individuals and is universally applicable to various types of complex traits including binary trait, quantitative trait, time-to-event trait, longitudinal traits, etc. For each genetic variant, SPAmix uses genotype data and genetic principal components (PCs) to estimate individual-level allele frequency, which is subsequently used to calibrate p values via a retrospective analysis. A hybrid strategy including saddlepoint approximation (SPA) can greatly increase the accuracy to analyze rare genetic variants, especially if the phenotypic distribution is unbalanced or extremely unbalanced. Compared to Tractor, SPAmix does not require local ancestry information and can be straightforwardly applicable to a multi-way admixed population. Meanwhile, SPAmix can also be extended to SPAmix<sub>local</sub> in which the local ancestry can be incorporated if available. In addition, we propose SPAmix<sub>CCT</sub> to combine the p values of SPAmix and SPAmix<sub>local</sub> via Cauchy combination (CCT). SPAmix<sub>local</sub> performs close to Tractor when analyzing quantitative traits and is more accurate when analyzing binary traits with an unbalanced case-control ratio. And SPAmix<sub>CCT </sub>is an optimal unified approach for various cross-ancestry genetic architectures. Extensive simulation studies and real data analyses of 369,314 UK Biobank individuals from multiple ancestries demonstrated that SPAmix is scalable and can discover novel hits while controlling type I error rates well.</p>
Data and analysis code from: Micro-scale geography of synchrony in a serpentine plant community
This package includes data and code to reproduce analyses of micro-scale geography of synchrony in the plant community at Jasper Ridge Biological Preserve. Plant cover and soil depth data come from long-term experimental plots established by Richard Hobbs. Plant cover is aggregated into 36 1m2 plots across three treatments (control, gopher exclosure, rabbit exclosure) from 1983 to 2015; included herein are data on the 6 most abundant species (Plantago erecta, Bromus hordeaceous, Lasthenia californica, Microseris douglasii, Vulpia microstachys, and Calycadenia multiglandulosa), total plant cover across all species, and records of gopher disturbance in the plots. The data package also includes time series of monthly precipitation and growing season Palmer’s Drought Severity Index for the same time period. An R Markdown file is included that reproduces all analyses described in the manuscript and reproduces all data figures. Data to support: Walter, Hallett et al. in review “Micro-scale geography of synchrony in a serpentine plant community
Large-scale longitudinal gradients of genetic diversity: a meta-analysis across six phyla in the Mediterranean basins
Predicting patterns of variation in biodiversity across the globe is a fundamental issue in ecology and evolution. Diversity within species, that is, genetic diversity, is of prime importance for understanding past and present evolutionary patterns, and highlighting areas where conservation might be a priority. However, most studies on spatial patterns of genetic diversity have not considered longitude as a potentially important ecological driver of these patterns. Therefore, we carried out a meta-analysis to examine the longitudinal patterns of genetic diversity in the Mediterranean Basin. Using published literature and a systematic review/meta-analysis framework, we collected data on the genetic diversity of species whose populations occur in the Mediterranean basin. We then calculated a coefficient of correlation between within‐population genetic diversity indices and longitude, and estimated the role of biological, ecological, biogeographic, and marker type factors on the strength and magnitude of this correlation in six phylla. The results of this study were published in the paper titled Large‐scale longitudinal gradients of genetic diversity: a meta‐analysis across six phyla in the Mediterranean basin (Conord et al. 2012).
MCR LTER: Coral Reef: Benthic Community Dynamics: Island Scale Coral Cover Analysis
These data describe the results of outer reef surveys that are conducted on an irregular schedule to quantify coral reef community dynamics on a scale of kilometers. The first island-wide survey was completed in 2006, and the second in 2010; we anticipate conducting such surveys every 5-6 years or as needed to capture the large-scale effects of disturbance regimes. The island-wide sampling adds 3 new sites per shore (for a total of 5 sites/shore with the fixed LTER sites) in each year that the larger scale analyses are completed.
Beneath the Antarctic sea-ice: Fine-scale analysis of Weddell seal (Leptonychotes weddellii) behaviour and predator-prey interactions, using micro-sonar data in Terre Adélie
<p>In this study, we tried to assess:<br>i) whether and how female Weddell seals feed (frequency, depth, duration) during lactation,<br>ii) what is their utilization of a limited foraging area (benthic or pelagic dives) as they are spatially constrained by the presence of their pup, and<br>iii) how can we characterize their foraging dives and the approach/catching phases using new tools providing a more detailed description of their behaviour.<br>Sonar tags were deployed on three breeding female Weddell seals in Terre Adélie (East Antarctica) in November 2019, to study animals' movements and dives at high resolution (3D acceleration, magnetometry, time and depth and GPS location), as well as information on prey and predator-prey interactions using acoustic data.</p>
Replication package: Dataset and stata-do-file for analysis in "Intragroup communication in social dilemmas: An artefactual public good field experiment in small-scale communities"
<p>This dataset was used for the analysis in "Intragroup communication in social dilemmas: An artefactual public good field experiment in small-scale communities". The data was collected in Namibia in 2017 as part of the SASSCAL research project by Nils Christian Hoenow and Adrian Pourviseh as members of the Chair for Development and Cooperative Economics at the University of Marburg. Funded by the Southern African Science Service Center for Climate Change and Adaptive Land-UseManagement (SASSCAL) through the German Federal Ministry for Education and Research (Grant No. 01LG1201B).</p> <p> </p> <p>Article Title: Intragroup communication in social dilemmas: An artefactual public good field experiment in small-scale communities </p> <p>Authors: Nils Christian Hoenow* and Adrian Pourviseh**</p> <p> </p> <p>*RWI – Leibniz Institute for Economic Research, Essen, Germany and & School of Business and Economics, University of<br>Marburg, Marburg, Germany</p> <p>**School of Business and Economics, University of<br>Marburg, Marburg, Germany</p> <p>Abstract: <br>Communication is well-known to increase cooperation rates in social dilemma situations, but the exact mechanisms behind this remain largely unclear. This study examines the impact of communication on public good provisioning in an artefactual field experiment conducted with 216 villagers from small, rural communities in northern Namibia. In line with previous experimental findings, we observe a strong increase in cooperation when face-to-face communication is allowed before decision-making. We additionally introduce a condition in which participants cannot discuss the dilemma but talk to their group members about an unrelated topic prior to learning about the<br>public good game. It turns out that this condition already leads to higher cooperation rates, albeit not as high as in the condition in which discussions about the social dilemma are possible. The setting in small communities also allows investigating the effects of pre-existing social relationships between group members and their interaction with communication.We find that both types of communication are primarily effective among socially more distant group members, which suggests that communication and social ties work as substitutes in increasing cooperation. Further analyses rule out better comprehension of the game and increased mutual expectations of one’s group members’ contributions as drivers for the communication effect. Finally, we discuss the role of personal and injunctive norms to keep commitments made during discussions.</p>
Data and analysis codes for "In vivo visualization of butterfly scale cell morphogenesis in Vanessa cardui"
<p>Butterfly scale data and data analysis codes for "In vivo visualization of butterfly scale cell morphogenesis in <em>Vanessa cardui</em>."</p> <p> </p> <p>It is recommended to download all files and folders into a single root folder for use in MATLAB.<br> This code was prepared for use in MATLAB R2019b, and some scripts or functions require the Image Processing Toolbox.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.