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10 results for “serial section reconstruction”
FIGURE 7 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 7. The state of preservation of the wax model of BP/1/1821. 1, the right side of the snout showing the deformation of the canine; 2, the palate showing the broken vomer; 3, the basicranium showing multiple cracks, nails and sticks around the pterygoid; 4, view of the right orbit showing multiple cracks, nails and sticks; 5, dorsal view of the of the mandible showing multiple cracks, nails and sticks; 6, detail of the inner side of the left ascending ramus of the mandible showing a stick embedded in the wax; and 7, lateral view of the mandible showing the incomplete reflected lamina of the angular. The arrows point to the nails, dotted lines demarcate the cracks. Not to scale.
FIGURE 6 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 6. Comparison between the digital model (top) and the wax model (bottom), in rostral (left) and lateral views (right). Arrows indicate the direction of deformation of the wax model. Scale bar equals 10 mm.
FIGURE 5 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 5. Segmentation of the skull bones of BP/1/1821, (1) in the cranium and (2) in the lower jaw. Scale bar equals 10 mm.
FIGURE 4 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 4. The slices of specimen BP/1/1821 finally put back together again, as seen in the hands of the CT scan facility technician K. Jakata (ESI). This 3D printed model is the first time in 55 years that BP/1/1821 has been accurately reconstructed at life-size.
FIGURE 3 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 3. The digital model in (1) right lateral, (2) left lateral and (3) ventral view in stereopairs. Scale bar equals 10 mm. See also the supplementary video in Appendix 2.
FIGURE 2 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 2. Comparison of the different reconstructions of BP/1/1821 in dorsal (left) and ventral (right) views. 1, the wax model; and 2, the original illustrations by Brink, found with the serial section drawings.
FIGURE 1 in Picking up the pieces: the digital reconstruction of a destroyed holotype from its serial section drawings
FIGURE 1. The label on the first page of the serial section drawings. It is written "Specimen N°1821 (FN). MN° 346. Complete Aneumogomphius? skull therocephalian. Sections ½ mm. Magnification x4." The document is signed "A.S. Brink". Abbreviations: FN, Field Number; MN°, Museum Number.
Study of Breast Cancer Shrinkage Modes After Neoadjuvant Chemotherapy With Whole-mount Serial Sections and Three-dimensional Pathological and MRI Reconstruction
ClinicalTrials.gov study NCT01917578. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Data from: Reconstruction of genetically identified neurons imaged by serial-section electron microscopy
Resolving patterns of synaptic connectivity in neural circuits currently requires serial section electron microscopy. However, complete circuit reconstruction is prohibitively slow and may not be necessary for many purposes such as comparing neuronal structure and connectivity among multiple animals. Here, we present an alternative strategy, targeted reconstruction of specific neuronal types. We used viral vectors to deliver peroxidase derivatives, which catalyze production of an electron-dense tracer, to genetically identified neurons, and developed a protocol that enhances the electron-density of the labeled cells and while retaining quality of the ultrastructure. The high contrast of the marked neurons enabled two innovations that dramatically speed data acquisition: targeted high-resolution reimaging of regions selected from rapidly-acquired lower resolution reconstruction, and an unsupervised segmentation algorithm. This pipeline reduces imaging and reconstruction times by at least two orders of magnitude, facilitating directed inquiry of circuit motifs.
Data from: Reconstruction of genetically identified neurons imaged by serial-section electron microscopy
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Allen Brain Atlas
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