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74 results for “silkworm”
Figure 2 in Isolation and characterization of bacteria associated with silkworm gut under antibiotic-treated larval feeding
Figure 2. Phylogenetic relationship and identification of bacterial strains isolated in this study based on 16S rRNA gene sequence through Neighbor Joining method using 1000 bootstrap replicates.
Рис. 2. Процесс поеΔания гусеницами искусственной питатеΛьной среΔы Fig. 2. Caterpillars feeding on artificial nutrient medium in Development dynamics of Kavkaz-2 and Sovetskaya-14 NGL silkworm caterpillars on artificial nutrient medium IPS 7.2-G
Рис. 2. Процесс поеΔания гусеницами искусственной питатеΛьной среΔы Fig. 2. Caterpillars feeding on artificial nutrient medium
Fig. 4 in Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
Fig. 4. IIM for placing silkworms on mulberry leaves. (A) One larva was placed on each leaf. (B) The larva eating the leaf. (C) The leaf after being eaten by the silkworm.
Fig. 3 in Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
Fig. 3. GIM for placing silkworms on mulberry leaves. (A) The leaves were arranged in the box after smearing them with BmNPV, and then they were air dried. (B) Five larvae were placed on each leaf. (C) The leaves after being eaten by the silkworms.
Figure 5 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions
Figure 5. TEM micrograph of chitosan nanoparticles prepared by ionic gelation method. Table 3. Antimicrobial activity of silver nanoparticles (µl) with different concentrations against fungal and bacterial isolates.
Figure 1 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions
Figure 1. Mean weight of healthy and infected fourth and fifth instar larvae of B. mori. Isolation and identification of bacterial isolates: Total of 7 bacterial were successfully isolated from the outer surface and the inner bоdy of silkworm larvae.
Figure 3 in Isolation and characterization of bacteria associated with silkworm gut under antibiotic-treated larval feeding
Figure 3. Phylogenetic relationship of bacterial strains isolated in this study with each other based on 16S rRNA gene sequence through Neighbor-Joining method using 1000 bootstrap replicates.
Figure 1 in Isolation and characterization of bacteria associated with silkworm gut under antibiotic-treated larval feeding
Figure 1. Amplification of 16S rRNA gene (1500 bp) of isolated bacterial strains; lane 1 = HG1, lane 2 = HG2, lane 3 = HG3, lane 4 = DG1, lane 5 = DG2, lane 6 = DG3, -ve = negative control, +ve = positive control, M = 1kb DNA marker.
Evaluation of the silkworm lemon mutant as an invertebrate animal model for human sepiapterin reductase deficiency
Human sepiapterin reductase deficiency is an inherited disease caused by SPR gene mutations and is a monoamine neurotransmitter disorder. Here, we investigated whether the silkworm lemon mutant could serve as a model of sepiapterin reductase deficiency. A point mutation in the BmSPR gene led to a five amino acid deletion at the carboxyl terminus in the lemon mutant. In addition, classical phenotypes seen in sepiapterin reductase deficient patients were observed in the lemon mutant, including a normal phenylalanine level, a decreased dopamine and serotonin content, and an increased neopterin level. A recovery test showed that replenishment of L-dopa significantly increased the dopamine level in the lemon mutant. The silkworm lemon mutant also showed negative behavioral abilities. These results suggest that the silkworm lemon mutant has an appropriate genetic basis and meets the biochemical requirements to be a model of sepiapterin reductase deficiency. Thus, the silkworm lemon mutant can serve as a candidate animal model of sepiapterin reductase deficiency, which may be helpful in facilitating accurate diagnosis and effective treatment options of sepiapterin reductase deficiency.
Evaluation of the silkworm lemon mutant as an invertebrate animal model for human sepiapterin reductase deficiency
<p><span>Human sepiapterin reductase deficiency is an inherited disease caused by <i>SPR</i> gene mutations and is a monoamine neurotransmitter disorder. Here, we investigated whether the silkworm <i>lemon<sup> </sup></i>mutant could serve as a model of sepiapterin reductase deficiency. A point mutation in the<i> BmSPR</i> gene led to a five amino acid deletion at the carboxyl terminus in the <i>lemon</i> mutant. In addition, classical phenotypes seen in sepiapterin reductase deficient patients were observed in the <i>lemon</i> mutant, including a normal phenylalanine level, a decreased dopamine and serotonin content, and an increased neopterin level. A recovery test showed that replenishment of L-dopa significantly increased the dopamine level in the <i>lemon<sup> </sup></i>mutant. The silkworm <i>lemon</i> mutant also showed negative behavioral abilities. These results suggest that the silkworm <i>lemon</i> mutant has an appropriate genetic basis and meets the biochemical requirements to be a model of sepiapterin reductase deficiency. Thus, the silkworm <i>lemon</i> mutant can serve as a candidate animal model of sepiapterin reductase deficiency, which may be helpful in facilitating accurate diagnosis and effective treatment options of sepiapterin reductase deficiency.</span></p>
Artificial selection on cis-element of Abl contributes cocoon yield increase in domestic silkworm
<p><span>The silkworm (</span><em><span>Bombyx mori</span></em><span>) is an important silk-producing domestic insect.</span><span> The quality and yield of the silk produced by <em>B. mori </em>exceeds that of its ancestor, the wild silkworm <em>B. mandarina</em>. However, to date little is known about the molecular mechanisms underlying domestication-related increases in silk yield. Here, we identified a gene associated with both domestication and silk-related quantitative trait locus (QTL): Abelson tyrosine protein kinase (<em>Abl</em>). </span><span>Population genomic data for <em>B. mori</em> and <em>B. mandarina</em> identified obvious signatures of artificial selection in the genomic region bearing the <em>Abl</em></span><span><em> </em>gene. T</span><span>here were two fixed nucleotide substitutions (−244 and −1311) in the </span><span>tran</span><span>scription factor binding motif of the upstream regulatory region of <em>B. mori Abl</em>.</span> <span>Compared to <em>Abl </em>in the wild silkworm <em>B. mandarina</em>, <em>B. mori Abl</em> exhibited significantly greater promoter activity and was upregulated in the silk gland from the last day of the 5<sup>th</sup> larval instar to pupal stage (P0). Compared to the wild type, CRISPR/Cas9-generated <em>Abl </em>loss-of-function mutants </span><span>exhibited a higher sensitivity to diseases, shorter developmental duration, and reductions in economically important silk traits, including cocoon weight, pupal weight, and cocoon layer thickness. Comparison of silk-gland transcriptomes between the wild type and the mutant indicated that genes enriched in ribosome biosynthesis, splicing, RNA transport, and the carbon metabolism were significantly downregulated in the mutants. Weighted gene co-expression network analysis (WGCNA) confirmed that genes related to ribosome biosynthesis were pivotal, driving the significant differentiation in silk yield between <em>B. mori</em> and <em>B. mandarina</em>. Here, we demonstrated that artificial selection acts on the cis-elements of silk-trait QTL gene <em>Abl </em>in a novel case (the domestic silkworm), and that this selection pressure increased <em>Abl </em>promoter activity and expression level. By promoting protein translation and synthesis, artificial selection further enhanced the robustness of silkworm larvae and improved cocoon silk synthesis.</span></p>
Figure 4 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions
Figure 4. Transmissiоn electron microscоpy micrоgraph of silver nanoparticles.
Figure 3 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions
Figure 3. Final dispersion formed after reduction (A) silver and (B) chitosan.
Nutritional Value Formation of Silkworm Pupa at Different Growth Stages: Analysis from Multi-omics Perspective
<p>Silkworm pupa (Bombyx mori) is affinal drug and diet in China, which is rich in protein and human essential amino acids. However, it is unclear how the internal nutritional composition changes and the antioxidant situation throughout the transformation of a silkworm pupa into a silkworm moth. In this study, we investigated the samples from four growth stages during silkworm pupal emergence by integrating proteomics and metabolomics. The results of the systematic bioinformatics study revealed that the growth of silkworm pupae considerably altered the ribosome-related genetic expression, cuticle development, and folate metabolism. The partial redox-active amino acid levels and the level of peroxiredoxin 1 expression were consistent with the fact that the total antioxidant capacity was higher in 7- and 10-day-old silkworm pupae. As compared to silkworm pupae, the amounts of fatty acids rose while those of amino acids, peptides, and organic acids dropped. This research also discovered that the altered protein profiles of silkworm pupae were controlled by the differential expression of mTOR signaling proteins (V-ATPase, S6, eIF4E) and ubiquitin mediate proteins (RP-L40e, UBE2L3, UBE2N, SKP1, ELOC). This study provided a new scientific foundation for the development and utilization of edible insect silkworm pupa functional food products.</p>
Knockdown of BmorCPR67 gene disrupts prepupal–pupal transition of silkworm Bombyx mori by thinning the endocuticle
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Evaluation of the silkworm lemon mutant as an invertebrate animal model for human sepiapterin reductase deficiency
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Artificial selection on cis-element of Abl contributes cocoon yield increase in domestic silkworm
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Evaluation of the silkworm lemon mutant as an invertebrate animal model for human sepiapterin reductase deficiency
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Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade
<p>Insect courtship and mating depend on integration of olfactory, visual, and tactile cues. Compared to other insects, <em>Bombyx mori</em>, the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas9 and electrophysiological techniques we found that courtship and mating behaviors are regulated in male silk moths by mutating genes in the sex determination cascade belonging to two conserved pathways. Loss of <em>Bmdsx</em> gene expression significantly reduced the peripheral perception of the major pheromone component bombykol by reducing expression of the product of the <em>BmOR1</em> gene which completely blocked courtship in adult males. Interestingly, we found that mating behavior was regulated independently by another sexual differentiation gene, <em>Bmfru</em>. Loss of <em>Bmfru</em> completely blocked mating, but males displayed normal courtship behavior. Lack of <em>Bmfru</em> expression significantly reduced the perception of the minor pheromone component bombykal due to the down regulation of <em>BmOR3</em> expression; further, functional analysis revealed that loss of the product of <em>BmOR3</em> played a key role in terminating male mating behavior. Our results suggest that <em>Bmdsx</em> and <em>Bmfru</em> are at the base of the two primary pathways that regulate olfactory-based sexual behavior.</p>
Effect of Silkworms (Bombyx Mori L.) Pupae Extracts on Musculoskeletal Biomarkers in Adults
ClinicalTrials.gov study NCT04994054. IPD Sharing: NO. Countries: 1. Publications: 1.
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Allen Brain Atlas
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