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11
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ShareScore release 0.9.0
Dataset results
11 results for “single-molecule tracking”
Data and code for "Tweezepy: A Python package for calibrating forces in single-molecule video-tracking instruments"
<p>Data and code for "Tweezepy: A Python package for calibrating forces in single-molecule video-tracking instruments."</p> <p>Data includes representative real and simulated bead trajectories used in the manuscript.</p> <p>Code includes all simulations, analysis, and plot details for the Figures in the manuscript. </p> <p>See included README.txt for more details.</p>
Imaging data from "Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD"
<p>3D 20ms, 3D 500ms and 2D dCas9 raw videos, localisation, tracking and trajectory analysis data</p> <p>From 'Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD" (2021). Biorxiv. https://doi.org/10.1101/2020.04.03.003178</p>
Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model
<p><span>The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated <em>in vivo</em>. Here, we visualize extracellular movement and binding of individual molecules of the activator-inhibitor signaling pair Nodal and Lefty in live developing zebrafish embryos using reflected light-sheet microscopy. We observe that diffusion coefficients of molecules are high in extracellular cavities, whereas mobility is reduced and bound fractions are high within cell-cell interfaces. Counterintuitively, molecules nevertheless accumulate in cavities, which we attribute to the geometry of the extracellular space by agent-based simulations. We further find that Nodal has a larger bound fraction than Lefty and shows a binding time of tens of seconds. Together, our measurements and simulations provide direct support for the hindered diffusion model and yield insights into the nanometer-to-micrometer-scale mechanisms that lead to macroscopic signal dispersal.</span></p>
Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model
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Example single molecule tracking data from: A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms
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Live-cell 3D single-molecule tracking reveals how NuRD modulates enhancer dynamics
GEO Series GSE179007. Mus musculus. 27 samples. Type: Other.
Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions
<p>for manuscript "Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions"</p>
Data belonging to "DNA-PAINT single-particle tracking (DNA-PAINT-SPT) enables extended single-molecule studies of membrane protein interactions"
<p>Source data for "DNA-PAINT single-particle tracking (DNA-PAINT-SPT) enables extended single-molecule studies of membrane protein interactions"</p>
Live-cell 3D single-molecule tracking reveals how NuRD modulates enhancer/promoter dynamics [ChIP-seq]
GEO Series GSE147789. Mus musculus. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions
GEO Series GSE263472. Schizosaccharomyces pombe. 5 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Exploring in vivo cholesterol-mediated interactions between activated EGF receptors in plasma membrane with single-molecule optical tracking
<p><strong>Background</strong>:: The first step in many cellular signaling processes occurs at various types of receptors in the plasma membrane. Membrane cholesterol can alter these signaling pathways of living cells. However, the process in which the interaction of activated receptors is modulated by cholesterol remains unclear.</p> <p><strong>Methods</strong>:: In this study, we measured single-molecule optical trajectories of epidermal growth factor receptors moving in the plasma membranes of two cancerous cell lines and one normal endothelial cell line. A stochastic model was developed and applied to identify critical information from single-molecule trajectories.</p> <p><strong>Results</strong>:: We discovered that unliganded epidermal growth factor receptors may reside nearby cholesterol-riched regions of the plasma membrane and can move into these lipid domains when subjected to ligand binding. The amount of membrane cholesterol considerably affects the stability of correlated motion of activated epidermal growth factor receptors.</p> <p><strong>Conclusions</strong>:: Our results provide single-molecule evidence of membrane cholesterol in regulating signaling receptors. Because the three cell lines used for this study are quite diverse, our results may be useful to shed light on the mechanism of cholesterol-mediated interaction between activated receptors in live cells. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.