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5,405 results for “skin”
Mueller matrix imaging combining optical parameters of mice non-melanoma skin cancer tissue
<p>The dataset consists of the Mueller matrix elements and optical parameters acquired from the backscattered light using a CCD camera and Mueller matrix imaging technique.</p><p>This dataset contains 90 samples including 20 feature vectors for SCC, 33 feature vectors for normal and 37 feature vectors for papilloma.</p>
Water restriction induces behavioral fight but impairs thermoregulation in a dry-skinned ectotherm
<p>Raw data of the article "Water restriction induces behavioral fight but impairs thermoregulation in a dry‐skinned ectotherm" by Rozen-Rechels D. et al., published in Oikos in 2020 (https://doi.org/10.1111/oik.06910). These data are freely available and are provided as csv files. Check the readme file for information on metadata.</p> <p>Data were formatted by David Rozen-Rechels and produced according to standards and protocols described in the companion paper.</p>
PsPM-SCBD: Skin conductance response from a delay fear conditioning task with auditory CS (monophones/triads)
<p>This dataset includes skin conductance response (SCR) measurements for 10 healthy unmedicated participants (5 females and 5 males, age range: 18 - 33 years, mean age: 24.1 +/- 4.7) participating in a classical (Pavlovian) discriminant delay fear conditioning experiment with auditory CS. Also included are CS and US information, and ratings of CS after the experiment. Simple and complex CS were simple sine tones (4 s), and triads in root position or in first inversion, respectively. US was a train of electric square pulses delivered with a constant current stimulator (Digitimer DS7A, Digitimer, Welwyn Garden City, UK) on participants' dominant forearm through a pin-cathode/ring-anode configuration. After the fear conditioning task, participants were first asked to report their subjective estimate of how likely they were to receive a shock after a given CS in the future, on a visual analogue scale of 0-100. Then they were asked to rate pairs of CS sounds with respect to which of the two stimuli they liked less.</p>
PsPM-SCRV6: Skin conductance responses to pain by electric stimulation
<p>This dataset includes skin conductance response (SCR) measurements for each of 20 healthy unmedicated participants (10 males and 10 females aged 21.8+/-3.3 years) in response to 10 discomforting electric shocks. Stimuli are 0.5ms wide square current pulse repeated at 500Hz for 100ms. Amplitude is varied (mean +/- SD: 0.78mA +/- 0.43mA). ITI is selected randomly on each trial from 29s, 34s or 39s.</p>
An experimental data set on the thermal and fluid dynamic performance of double skin facades (DSFs) subjected to various controlled boundary conditions through the use of a climate simulator facility
<p>Double skin facades (DSFs) are building envelope systems defined by complex phenomena and non-linear-processes that make characterizing their performance a non-trivial task. In an effort to enable the scientific community to access experimental data for further analysis or model validation purposes, we release together with the open-access paper entitled “<strong><em>Laboratory testbed and methods for flexible characterization of the thermal and fluid dynamic behavior of double skin facades” (</em></strong><a href="https://doi.org/10.1016/j.buildenv.2021.108700"><strong><em>https://doi.org/10.1016/j.buildenv.2021.108700</em></strong></a><strong><em>)</em></strong>, a set of experimental data collected during tests carried out with the use of the newly developed testbed. The data contains the results of a series of tests where various configurations of a full-scale DSF mock-up that have been subjected to different boundary conditions replicated in a climate simulator. The database contains a guide in the form of the file ‘Guide.pdf’, which explains how to read data, presents a schematic drawing of sensor layout, and provides more information on sensors’ positions. Further information on the original aims of the experiments, methods, and other data can be found in the article mentioned above, which becomes an essential tool to understand how to read and interpret the experimental data fully. The following collection of experimental data are provided:</p> <ul> <li>32 steady-state measurements where the following factors were changed: ventilation mode (indoor and outdoor air curtain), solar irradiance (0, 400, 600, and 800 Wm<sup>-2</sup>), outdoor chamber temperature (10, 20, 30, and 40 ℃), cavity depth (20, 30, 40 and 60 cm) and venetian blinds position (no blinds, closed blinds, θ=45 º, and open blinds) [file names: ‘Taguchi_4Lx4F_L16_I-I.csv’ and ‘Taguchi 4Lx4F_L16_O-O.csv’],</li> <li>Dynamic profile measurements corresponding to a typical hot summer day [Dynamic_profile_measurements.csv] and</li> <li>Calibration data [Callibration.csv].</li> </ul> <p>Any inquires on the experimental data<em> can be sent </em>to: aleksandar.jankovic@ntnu.no</p>
An experimental data set for the analysis of the thermophysical behavior of a single-story naturally ventilated double-skin façade (DSF) under summer boundary conditions
<p>Double-skin facades (DSFs) are adaptive building envelope elements that offer the possibility to dynamically interact with the heat and mass flow between indoor and outdoor environments. Though designed to provide better performance compared to more conventional envelope solutions, these façade systems may, in some cases, underperform and lead to an increase in energy use or in thermal discomfort if not properly designed and operated. One of the known problems is the risk of overheating, in hot periods, in the ventilated cavity. In order to analyze this effect, we have systematically investigated the performance of a single-story, naturally ventilated DSF. The DSF is operated in the so-called outdoor air curtain mode and has venetian blinds installed in the 20 mm deep ventilated cavity. Tests were carried out under a steady-state regime corresponding to relevant summertime conditions. In an effort to enable the scientific community to access experimental data to analyze this problem further or for model validation purposes, we released together with the open-access paper entitled "<strong>Characterization of a naturally ventilated double-skin façade through the design of experiments (DOE) methodology in a controlled environment</strong>," the entire set of experimental data collected during the tests. The data set contains the results of a series of experimental runs where different configurations of the DSF, as detailed below, have been subjected to various boundary conditions through a climate simulator facility equipped with a solar simulator device. The database is supported by a guide ("Guide.pdf"), where further explanations about how to read data and schematic drawings of the sensor layout are provided. Additional information about the original aims of the experiments, the detailed methods, and other data processing procedures can be found in the article mentioned above. The collection of experimental tests in this data set covers:</p> <ul> <li>49 steady-state measurements where the following factors were changed using different experimental designs: solar irradiance (0, 350, and 700 Wm<sup>-2</sup>), outdoor chamber temperature (15, 25, and 35 ℃), opening size (7, 21, and 42 dm<sup>2</sup>), and venetian blinds angle (closed blinds θ=0 º, θ=45 º, and open blinds θ=90 º) [file name: "Complete_data.csv"],</li> </ul> <p>Any inquiries about the experimental data can be sent to: <a href="mailto:aleksandar.jankovic@ntnu.no">aleksandar.jankovic@ntnu.no</a></p> <p>The activities presented in this paper were carried out within the research project "REsponsive, INtegrated, VENTilated - REINVENT – windows," supported by the Research Council of Norway through the research grant 262198, and the partners SINTEF, Hydro Extruded Solutions, Politecnico di Torino and Aalto University.</p>
De Obaldia et al. Differential mosquito attraction to humans is associated with skin-derived carboxylic acid levels
<p>These supplementary files accompany the manuscript by De Obaldia et al. entitled "Differential mosquito attraction to humans is associated with skin-derived carboxylic acid levels." This includes all raw data in the paper, supplementary data, and instructions for the mosquito behavioral assays.</p> <p> </p> <p>On January 2, 2023 we added one new data file and a .readme to explain changes between the original pre-print and the published peer-reviewed version of the paper https://pubmed.ncbi.nlm.nih.gov/36261039/</p>
Skin-blubber biopsy samples and associated demographic data collected from cetaceans encountered along the Western Antarctic Peninsula (WAP), 2010 – 2024
Baleen whale populations in the Southern Ocean are recovering after intense commercial whaling in the 20th century. Along the Western Antarctic Peninsula (WAP), this recovery is occurring in one of the planet's most rapidly changing marine ecosystems. Understanding how climate-driven changes influence the population dynamics of whales in this region is critical for understanding what conservation and management actions must be prioritized to maintain the structure and function of this marine ecosystem. To begin understanding the dynamics of whale recovery under continued environmental change, we need to study these whales' demography and population dynamics. As part of our annual sampling surveys for cetaceans along the WAP through the PAL LTER program, we actively collect remote non-lethal skin-blubber biopsy samples and have developed one of the most extensive tissue archives in the Southern Ocean. With these samples, we conduct a series of demographic and physiological measurements. Using the skin portion of the biopsy sample, we isolate nuclear and mitochondrial DNA (mtDNA) to develop a DNA profile for each sample, including genetic sex, a microsatellite genotype, and a mtDNA haplotype. These profiles are used to compare sex ratios of the population, determine individual recaptures through genotype analysis, and better understand population mixing. Using the blubber portion of the biopsy sample, we isolate endocrine markers (e.g., progesterone and cortisol) to monitor population pregnancy rates and stress levels. This data represents some of the first non-lethal quantitative observations of the demography and population dynamics of recovering whale populations in the Antarctic and provides a critical reference point for future work as the Antarctic climate continues to change and populations continue to recover from whaling.
Supplementary data for "Armored with skin and bone: A combined histological and µCT study of the exceptional integument of the Antsingy leaf chameleon Brookesia perarmata (Angel, 1933)"
<p>This project contains the supplementary µCT-scans of the whole body and a lateral flank integumentary armor of <em>Brookesia perarmata</em> (Angel, 1933) (Squamata: Iguania: Chamaeleonidae) belonging to the following publication:</p> <p>Schucht P, Rühr PT, Geier B, Glaw F & M LAmbertz (<strong>2020</strong>): Armored with skin and bone: A combined histological and µCT study of the exceptional integument of the Antsingy leaf chameleon <em>Brookesia perarmata</em> (Angel, 1933). <em>Journal of Morphology</em>. doi: <a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jmor.21135">10.1002/jmor.21135</a>.</p> <p> </p> <p><strong>Whole body scan:</strong></p> <ul> <li>specimen: ZSM 17/2006, Zoologische Staatssammlung München</li> <li>machine: phoenix nanotom m (GE Measurement & Control)</li> <li>scan settings: <ul> <li>tube voltage = 110 kV</li> <li>ube current = 70 μA</li> <li>target = tungsten</li> <li>no filter</li> <li>total sample rotation = 360°</li> <li>angular step size = 0.24°</li> <li>exposure time = 750 ms</li> <li>binning = 1</li> <li>averaging = 4</li> <li>voxel size = 37.8 μm</li> </ul> </li> <li>filename: Schucht_B_perarmata_whole.tif</li> </ul> <p> </p> <p><strong>Lateral flank integumentary armor scan:</strong></p> <ul> <li>specimen: ZSM 862/2000, Zoologische Staatssammlung München</li> <li>machine: Skyscan 1272 device (Bruker microCT)</li> <li>scan settings: <ul> <li>tube voltage = 70 kV</li> <li>ube current = 142 μA</li> <li>target = tungsten</li> <li>filter = Al 0.5 mm</li> <li>total sample rotation = 180°</li> <li>angular step size = 0.19°</li> <li>exposure time = 1925 ms</li> <li>binning = 2x2</li> <li>averaging = 8</li> <li>random movement = 15</li> <li>voxel size = 4.4 μm</li> </ul> </li> <li>filename: Schucht_B_perarmata_osteoderm.tif</li> </ul>
New Generation UV-A Filters: Understanding Their Photodynamics on a Human Skin Mimic
<p>The sparsity of efficient commercial ultraviolet-A (UV-A) filters is a major challenge towards developing effective broadband sunscreens with minimal human- and eco-toxicity. To combat this, we have designed a new class of Meldrum-based phenolic UV-A filters. We explore the ultrafast photodynamics of coumaryl Meldrum, CMe, and sinapyl Meldrum, SMe, both in an industry standard emollient and on a synthetic skin mimic, using femtosecond transient electronic and vibrational absorption spectroscopies, and computational simulations. Upon photoexcitation to the lowest excited singlet state (S<sub>1</sub>), these Meldrum-based phenolics undergo fast and efficient non-radiative decay to repopulate the electronic ground state (S<sub>0</sub>). We propose an initial ultrafast twisted intramolecular charge transfer mechanism as these systems evolve out of the Franck-Condon region towards an S<sub>1</sub>/S<sub>0</sub> conical intersection, followed by internal conversion to S<sub>0</sub> and subsequent vibrational cooling. Importantly, we correlate these findings to their long-term photostability upon irradiation with a solar simulator and conclude that these molecules surpass the basic requirements of an industry standard UV filter.</p>
Effective de-icing skin using graphene-based flexible heater
<p>dataset on </p> <p>Dynamic Mechanical Analysis, Electro-Mechanical Measurement, Dynamic Light Scattering</p> <p>FTIR spectroscopy, Thermogravimetric analysis, Differential Scanning Calorimetry,</p> <p>Electro-Temperature Measurement, Thermal Image Camera, Water sorption measurement,</p> <p>Transmission Electron Microscopy and Stress Strain</p>
Skin response measurements
<p>The objective of this study is to investigate the possibility to detect mental and light physical stress through the measurement of skin reflectance in the mm-wave/sub-THz band. Two frequency bands have been considered, 75-110 GHz (Band-I) and 325-500 GHz (Band-II), while the measurements have been performed in the three different locations, the arm, the dorsal side of the hand and the fingertip.</p> <p>Each transmitter and receiver set is equipped with a matched standard horn antenna operated at the selected frequency band.</p> <p>The transmitter and receiver used during the test are active mixers. To operate each active module a local oscillator (LO) is required. The PXA and the Agilent generators are used as LO for transmitter (TX) and receiver (RX) accordingly (Fig. 2). The output signal from the receiver (RX) is gathered by a spectrum analyzer and then post-processed. The entire lab bench is connected through GPIB and both LO generators are synchronized (10MHz).</p> <p>The measured reflected signal amplitude is recorded and post-process on a personal computer.</p> <p>The dataset refers to Laboratory test. The skin reflectance was measured during rest and after mental and physical stress. Physical stress was provoked using a dynamometer under 15 N of force for 5 minutes. Provocation of mental stress was achieved with the use of the Stroop Test [11] for 15 minutes. A stress measurement was always preceded by a resting period of at least 15 minutes. Three hand locations have been considered, (a) arm, (b) hand and (c) finger.</p> <p>The datasets are related to the open accessible publication "<strong>Human Physical Condition RF Sensing at THz range".</strong></p>
PsPM-SCB2D: Skin conductance response from a delay fear conditioning task with auditory CS (monophones/triads)
<p>This dataset includes skin conductance response (SCR) for 22 healthy unmedicated participants (15 females and 7 males, age range: 18 - 32 years, mean age: 22.1 +/- 3.4) participating in a classical (Pavlovian) discriminant delay fear conditioning experiment with auditory CS. Also included are CS and US information, and ratings of CS after the experiment. Simple and complex CS were simple sine tones (4 s), and triads in root position or in first inversion, respectively. US was a train of electric square pulses delivered with a constant current stimulator (Digitimer DS7A, Digitimer, Welwyn Garden City, UK) on participants' dominant forearm through a pin-cathode/ring-anode configuration. After the fear conditioning task, participants were first asked to report their subjective estimate of how likely they were to receive a shock after a given CS in the future, on a visual analogue scale of 0-100. Then they were asked to rate pairs of CS sounds with respect to which of the two stimuli they liked less.</p>
The official dataset of the papper " Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics"
<p>This is the official repository of the paper "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics" (https://doi.org/10.1080/15538362.2024.2322743)</p> <p>DISCLAIMER</p> <p>The repository contains experimental data and is published for the sole purpose of giving additional background details on the respective publication "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics" (https://doi.org/10.1080/15538362.2024.2322743).<strong> </strong>See the README.txt file for more details.</p>
SkinSource: A Data-Driven Toolbox for Predicting Touch-Elicited Skin Vibrations Across the Upper Limb
<p>The repository contains the data for the toolbox released as part of the publication “SkinSource: A Data-Driven Toolbox for Predicting Touch-Elicited Vibrations in the Upper Limb.” The toolbox and installation and usage instructions can be found on GitHub here: <a href="https://github.com/neelitummala/skinsource">https://github.com/neelitummala/skinsource</a>. If you use these data or our toolbox please cite our publication: <a href="https://doi.org/10.1109/HAPTICS59260.2024.10520852">https://doi.org/10.1109/HAPTICS59260.2024.10520852</a>.</p> <p>Full citation: “Tummala, N., Reardon, G., Fani, S., Goetz, D., Bianchi, M., and Visell, Y. (2024) SkinSource: A Data-Driven Toolbox for Predicting Touch-Elicited Vibrations in the Upper Limb. IEEE Haptics Symposium 2024. DOI: 10.1109/HAPTICS59260.2024.10520852” </p> <p> </p> <p><strong>Abstract From Manuscript</strong></p> <p>Vibrations transmitted throughout the hand and arm during touch contact play a central role in haptic science and engineering but are challenging to model or experimentally characterize. Here, we present SkinSource, a data-driven toolbox for predicting skin vibrations across the upper limb in response to user-specified input forces. The toolbox leverages impulse response measurements that encode the physics of vibration transmission across the hands and arms of four participants and provides software tools for analyzing the predicted skin responses. We show that the SkinSource predictions closely match experimental measurements and confirm the underlying assumption of linear vibration transmission in the skin. We also demonstrate through several usage examples how SkinSource can act as a versatile computational platform for haptic research applications, such as characterizing vibrotactile transmission in the skin, engineering haptic interfaces, and investigating touch perception.</p> <p><strong> </strong></p> <p><strong>Dataset Description</strong></p> <p>This dataset comprises experimental data of 3-axis surface acceleration at 72 locations on the skin in response to unit impulsive forces supplied at 20 different input locations on the palmar hand surface. For details on our experimental procedure, please see our publication. This data is intended to be used as part of the SkinSource toolbox, which can be found here: <a href="https://github.com/neelitummala/skinsource">https://github.com/neelitummala/skinsource</a>.</p> <p><strong> </strong></p> <p><strong>Data Fields</strong></p> <p>The data is provided as a .mat file. This file contains a single variable “dataTable” of variable type “table.” The table contains 80 rows, each corresponding to a unique experimental condition (4 participants x 20 input locations), and contains the following fields:</p> <p><strong>Data </strong>(522x72x3) - 3D array containing the 3-axis skin acceleration at 522 time points (impulse responses) for each of 72 accelerometers. Please see the GitHub code and documentation (<a href="https://github.com/neelitummala/skinsource">https://github.com/neelitummala/skinsource</a>) for the accelerometer locations on the dorsal surface of the upper limb.</p> <p><strong>Model </strong>- The upper limb model number. This number specifies the participant that data was taken on.</p> <p><strong>Location</strong> -<strong> </strong>Number designating which input location on the palmar hand surface the data corresponds to. Please see the GitHub code and documentation (<a href="https://github.com/neelitummala/skinsource">https://github.com/neelitummala/skinsource</a>) for input location number mapping.</p>
Supramolecular Self-Healing Sensor Fiber Composites for Damage Detection in Piezoresistive Electronic Skin for Soft Robots
<p>Self-healing materials can prolong the lifetime of structures and products by enabling the repairing of damage. However, detecting the damage and the progress of the healing process remains an important issue. In this study, self-healing, piezoresistive strain sensor fibers (ShSFs) are used for detecting strain deformation and damage in a self-healing elastomeric matrix. The ShSFs were embedded in the self-healing matrix for the development of self-healing sensor fiber composites (ShSFC) with elongation at break values of up to 100%. A quadruple hydrogen-bonded supramolecular elastomer was used as a matrix material. The ShSFCs exhibited a reproducible and monotonic response. The ShSFCs were investigated for use as sensorized electronic skin on 3D-printed soft robotic modules, such as bending actuators. Depending on the bending actuator module, the electronic skin was loaded under either compression (pneumatic-based module) or tension (tendon-based module). In both configurations, the ShSFs could be successfully used as deformation sensors, and in addition, detect the presence of damage based on the sensor signal drift. The sensor under tension showed better recovery of the signal after healing, and smaller signal relaxation. Even with the complete severing of the fiber, the piezoresistive properties returned after the healing, but in that case, thermal heat treatment was required. With their resilient response and self-healing properties, the supramolecular fiber composites can be used for the next generation of soft robotic modules</p>
Assessment of skin autofluorescence and its association with glycated hemoglobin, cardiovascular risk markers and concomitant chronic diseases in children with type 1 diabetes
<p>This is the dataset for the publication "Assessment of skin autofluorescence and its association with glycated hemoglobin, cardiovascular risk markers and concomitant chronic diseases in children with type 1 diabetes".</p>
LakeSST: Lake Skin Surface Temperatures in French inland water bodies
<p>The data set LakeSST contains skin surface temperature data for 442 French water bodies for the period 1999-2016 obtained from archives of Landsat 5 and Landsat 7 thermal infrared images. The overall accuracy of the satellite-derived temperature measurements is about 1.2 ºC, similar to other applications of satellite images to estimate freshwater surface temperatures. The spatial and temporal coverage of the data set makes it an ideal resource for studies on the temporal evolution of lake surface temperatures and for geographical studies of temperature patterns.</p>
The microclimate, surface energy flux and human skin burn risks of artificial turf as compared to natural turf
<p>This dataset contains the measured hourly mean microclimate and surface energy flux data from a field experiment. The experiment consisted of three treatments: unirrigated artificial turf, unirrigated natural turf, and irrigated natural turf (4 mm/day, 13:00-13:23 local time). The experiment was conducted from 2024-01-28 to 2024-03-18 in Burnley, Melbourne, Australia.<br><br>For each treatment, the measured hourly mean data included albedo, soil moisture content, air temperature, vapour pressure of water, wind speed, black globe temperature, mean radiant temperature, universal theraml climate index, wet-bulb globe temperature, soil temperature, turf surface temperature, incoming and outgoing longwave and shortwave radiant fluxes, sensible heat flux, latent heat flux, and ground heat flux. <br><br>Turf surface temperature, and incoming and outgoing longwave and shortwave radiant fluxes were measured at 1.5 m above ground surface.<br>Air temperature and vapour pressure of water were measured at 0.6 and 1.1 m above ground surface.<br>Wind speed, black globe temperature, mean radiant temperature, universal thermal climate index, and wet-bulb globe temperature were measured at 1.1 m above ground surface.<br>Soil moisture content, soil temperature and ground heat flux were measured at 0.1 m below ground surface.<br>Sensible heat flux and latent heat flux were calculated using the Bowen ratio-energy balance method.<br><br>Additionally, the hourly mean background weather conditions (air temperature and cloud amount) from the nearest public climate station in the study period were included in 'ReferenceClimateStation.csv'. Hourly total rainfall data measured at the study site was also included. <br><br>The aims of this study was to:<br>1. Compare the microclimate and human heat stress among the three treatments.<br>2. Assess and compare the human skin burn risks of the three treaments from their turf surface temperatures.<br>3. Analyse the surface energy fluxes of the three treatments to identify the mechanisms by which artificial turf develops any microclimate, human heat stress and turf surface temperature differences.<br><br>This study was published in:</p> <p><span>Cheung, P. K., & Livesley, S. J. (2025). The microclimate, surface energy flux and human skin burn risks of artificial turf as compared to natural turf. <em>Building and Environment</em>, 112679. https://doi.org/10.1016/j.buildenv.2025.112679<br></span><br>Contact person: Dr Paul Cheung (cheung.p@unimelb.edu.au)</p>
Deconvolved STED nanoscopy images of the nuclear phosphatidylinositol 4,5-bisphosphate and nuclear speckle marker SON together with deconvolved confocal images of DAPI stained nuclei in human formalin-fixed paraffin-embedded skin warts sections
<p>The collection and analysis of formalin-fixed paraffin-embedded (FFPE) human skin sections was approved by the local ethics-committee at the Department of Pathology, University of Cologne, Germany. Written informed consentwas obtained from all patients in accordance with the Declaration of Helsinki. For biopsy materials from archival paraffin blocks of human skin, an informed consent was obtained from all the subjects and ethical approval obtained from the Ethics Committee at the University of Cologne. Surgically removed human FFPE skin biopsies were sectioned into 4 µm sections. Sections were dewaxed, and indirectly immunofluorescently labeled against nuclear phosphatidylinositol 4,5-bisphosphate (nPI(4,5)P2) using 5 µg/mL rabbit primary polyclonal antibody (Echelon Biosciences Inc. Z-A045, clone 2C11). The primary antibody against nPI(4,5)P2 was recognized by the goat secondary antibody conjugated with Abberrior Star 635P (Abberior 2-0002-007-5). Sections were indirectly immunofluorescently labeled against nuclear speckle marker SON using 1 µg/mL rabbit primary polyclonal antibody (Abcam ab121759). The primary antibody against SON was recognized by the goat secondary antibody conjugated with Abberrior Star 580 (Abberrior ST580-1002). Sections were co-stained by DAPI 1:1000 in PBS for 5 min.</p> <p>Imaging of nPI(4,5)P2-635P channel was performed on Leica TCS SP8 STED 3x inverted DMi8 microscope with pulsed white light laser 470-640 nm 1.5 mW and 775 nm pulse STED laser >1.5 W controlled by Leica Application Suite X software and equipped with HC PL APO CS2 100x/1.40 OIL objective used with Leica Type F immersion oil n=1.518. Unidirectional xyz scanning speed was 400 Hz, line accumulation 8. Pixel size was 20 nm in X and Y. Channel settings: 7% 633 nm laser; 775 Notch filter; 50% 775 nm STED laser; 30% 3D STED; HyD 639-698 nm, photon-counting mode, gain 100, gating 0.3-10 ns. Imaging of SON-580 channel was performed on Leica TCS SP8 STED 3x inverted DMi8 microscope with pulsed white light laser 470-640 nm 1.5 mW and 775 nm pulse STED laser >1.5 W controlled by Leica Application Suite X software and equipped with HC PL APO CS2 100x/1.40 OIL objective used with Leica Type F immersion oil n=1.518. Unidirectional xyz scanning speed was 400 Hz, line accumulation 8. Pixel size was 20 nm in X and Y. Channel settings: 10% 585 nm laser; 775 Notch filter; 80% 775 nm STED laser, 30% 3D STED; Hybrid detector (HyD) 589-616 nm, photon-counting mode, gain 100, gating 0.4-10 ns.</p> <p>Z-stacks of STED images were deconvolved using Huygens Professional 22.10 software (Scientific Imaging B.V.). Data sets were processed using Workflow Processor. The workflow consisted of selecting images, setting up the microscopy and deconvolution parameters and saving deconvolved images as 8-bit TIFF single files for individual channels (which were later used for the quantitative analyses; see below). Microscopy parameters were optimized and set as follows. Sampling intervals were ≤20 nm in X and Y and ≤20 nm in Z. Numerical aperture was 1.4; refractive indexes of the lens immersion oil was 1.518 and of the embedding media 1.458; objective quality was good, coverslip position was 0 µm and imaging direction was downward. For nPI(4,5)P2-635P STED channel the backprojected pinhole was 216 nm; excitation (ex.) and emission (em.) wavelengths (λ) were 633 and 651 nm, resp., ex. fill factor 2. STED depletion mode was pulsed, saturation factor 25, STED λ = 775, STED immunity factor 10 and STED 3X was 30%. Classic MLE algorithm with stabilization of Z-slices was used and signal-to-noise ratio was 5.1. For SON-580 STED channel the backprojected pinhole was 195 nm; excitation (ex.) and emission (em.) wavelengths (λ) were 585 and 602 nm, resp., ex. fill factor 2. STED depletion mode was pulsed, saturation factor 20, STED λ = 775, STED immunity factor 10 and STED 3X was 30%. Classic MLE algorithm with stabilization of Z-slices was used and signal-to-noise ratio was 4.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.