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20
datasets available to search
ShareScore release 0.9.0
Dataset results
20 results for “slice culture”
Using cultured canine cardiac slices to model the autophagic flux with doxorubicin
<p>Chemotherapy-induced impairment of autophagy is implicated in cardiac toxicity induced by anti-cancer drugs. Imperfect translation from rodent models and lack of in vitro models of toxicity has limited investigation of autophagic flux dysregulation, preventing design of novel cardioprotective strategies based on autophagy control. Development of an adult heart tissue culture technique from a translational model will improve investigation of cardiac toxicity. We aimed to optimize a canine cardiac slice culture system for exploration of cancer therapy impact on intact cardiac tissue, creating a translatable model that maintains autophagy in culture and is amenable to autophagy modulation. Canine cardiac tissue slices (350 μm) were generated from left ventricular free wall collected from euthanized client-owned dogs (n=7) free of cardiovascular disease at the Foster Hospital for Small Animals at Tufts University. Cell viability and apoptosis were quantified with MTT assay and TUNEL staining. Cardiac slices were challenged with doxorubicin and an autophagy activator (rapamycin) or inhibitor (chloroquine). Autophagic flux components (LC3, p62) were quantified by western blot. Cardiac slices retained high cell viability for >7 days in culture and basal levels of autophagic markers remained unchanged. Doxorubicin treatment resulted in perturbation of the autophagic flux and cell death, while rapamycin co-treatment restored normal autophagic flux and maintained cell survival. We developed an adult canine cardiac slice culture system appropriate for studying the effects of autophagic flux that may be applicable to drug toxicity evaluations.</p>
Using cultured canine cardiac slices to model the autophagic flux with doxorubicin
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Dataset related to article "A novel organotypic cortical slice culture model for traumatic brain injury: molecular changes induced by injury and mesenchymal stromal cell secretome treatment"
<p>Dataset related to manuscript "A novel organotypic cortical slice culture model for traumatic brain injury: molecular changes induced by injury and mesenchymal stromal cell secretome treatment"</p> <p>Each excel file contains raw data relative to data shown in Figures</p>
Benefit and limitations of glioblastoma tissue slice cultures – an RNA sequencing study
GEO Series GSE179649. Homo sapiens. 97 samples. Type: Expression profiling by high throughput sequencing.
Prostate cancer organotypic slice cultures (OSC) treated with E2 or DHT
GEO Series GSE142287. Homo sapiens. 2 samples. Type: Expression profiling by array.
Transcriptional changes of precision-cut liver slices after 3 days of cultures in the absence or presence of 2.5 mM valproic acid sodium salt.
GEO Series GSE194128. Mus musculus. 7 samples. Type: Expression profiling by high throughput sequencing.
Single cell RNA-sequencing of long-term human control and C9ORF72 ALS/FTD patient-specific iPSC-derived cortical organoid slice cultures
GEO Series GSE180122. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Organotypic brain slice culture microglia exhibit molecular similarity to acutely-isolated adult microglia and provide a platform to study neuroinflammation
GEO Series GSE154855. Mus musculus. 55 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA-seq of astrocytes and neurons from primary culture of the mouse brain and of acute slice of interneurons from Patch-clamp pipette [RNA-seq mouse brain]
GEO Series GSE231725. Mus musculus. 28 samples. Type: Expression profiling by high throughput sequencing.
Non-productive infection of glial cells with SARS-CoV-2 in hamster organotypic cerebellar slice cultures
GEO Series GSE201921. Mesocricetus auratus. 17 samples. Type: Expression profiling by high throughput sequencing.
Ovarian Tumor Organotypic Slices Cultures for functionAl Drug Testing and Therapy Response Prediction
ClinicalTrials.gov study NCT06065358. IPD Sharing: NO. Countries: 1. Publications: 0.
Data from: Long-term adult human brain slice cultures as a model system to study human CNS circuitry and disease
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Deep sequencing and automated histochemistry of human tissue slice cultures improve their usability as preclinical model for cancer research
GEO Series GSE119102. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Vehicle and sAPPalpha-treated hippocampal slice cultures
GEO Series GSE1555. Mus musculus. 6 samples. Type: Expression profiling by array.
Anti-Nogo-A antibody treatment of Rat Hippocampal Slice Cultures
GEO Series GSE12483. Rattus norvegicus. 12 samples. Type: Expression profiling by array.
Gene expression in response to axotomy in organotypical slices co-cultures
GEO Series GSE20528. Rattus norvegicus. 12 samples. Type: Expression profiling by array.
A Physiological Biomimetic Culture System for Pig and Human Heart Slices
GEO Series GSE133723. Sus scrofa. 11 samples. Type: Expression profiling by high throughput sequencing.
Time course of prion infected (RML) and globular domain ligands (POM1) in cultured organotypic cerebellar slices (COCS)
GEO Series GSE65147. Mus musculus. 72 samples. Type: Expression profiling by array.
RNAseq from pig heart slices cultured in CTCM for 12 days
GEO Series GSE211110. Sus scrofa. 14 samples. Type: Expression profiling by high throughput sequencing.
Entorhino-hippocampal slice culture on 3D-printed silk fibroin insert
<p>20230406_LM_slice_culture: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 3.0X) of cultured entorhino-hippocampal slice cultures (22 days) on the metal frame spider silk.</p> <p>20230426_CM_slice_culture: a TIF-file showing an LSM-image (Leica TCS SP8, 20X (NA 0.75;Leica)) of PI and DAPI staining of hippocampal-entorhinal cortical slices (22 days in vitro) on the metal frame spider silk.</p> <p>20230522_LM_slice_culture01: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 2.0X) of entorhino-hippocampal slice culture (7 days in vitro) on the 3D-printed insert.</p> <p>20230627_LM_slice_culture02: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 4.0X) of entorhino-hippocampal slice culture (5 days invitro) on the 3D-printed insert.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.