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40 results for “soil microbial activity”
Temperature Sensitivity of Microbial Activity in Three Forest Soils at Harvard Forest 2010-2011
We evaluated possible seasonal variation in the temperature sensitivity of microbially mediated soil fluxes related to decomposition (net N mineralization, net nitrification, proteolysis, the maximum velocity (Vmax) of proteolysis, microbial respiration, and the Vmax of four soil exo-enzymes) across forests dominated by eastern hemlock (Tsuga canadensis), white ash (Fraxinus americana), and red oak (Quercus rubra) in Harvard Forest. We asked two simple questions: (1) do temperature sensitivities vary across forest types or different steps of the decomposition process, and (2) do temperature sensitivities display plasticity on a seasonal time frame?We observed substantial variation in temperature sensitivities (Q10 and R10 values) across the different fluxes and forest types. The ash soils exhibited the strongest temperature sensitivities and the mineral-N fluxes exhibited higher temperature sensitivities relative to the proteolytic fluxes or microbial respiration. The Vmax of soil exo-enzymes varied considerably in an interactive manner across forests and time, and the response of some enzymes was consistent with the thermal plasticity. The enzymatic kinetic properties Vmax and Km (half-saturation constant) were strongly correlated with slopes that differed across enzymes, reflecting an enzyme-specific tradeoff between maximum catalytic rate and substrate-binding efficiency. Generally, Q10 values were largely constant, but R10 values varied in a manner consistent with distinct seasonal plasticity. There was a consistent seasonal shift in R10 values coincident with snowmelt, suggesting that the time following snowmelt is a particularly interesting and dynamic period of microbial activity in these temperate forests.
Physical soil characteristics, microbial community composition, extracellular enzymatic activity, biologically based phosphorus (BBP) pools, and available phosphorus from two soil depths, four microhabitats, and four landforms at the Jornada Experimental Range, 2021.
This dataset contains physical soil characteristics, PLFA based microbial community composition, extracellular enzymatic activity, nitrate and ammonium activity, and phosphorus availability in various phosphorus pools (Biologically Based Phosphorus, potassium sulfate, Olsen-P). Soils were collected from two depths (0-2cm, 2-30 cm), four microhabitats (grass, shrub, biocrust, interspace), and four landforms (alluvial flat, alluvial fan remnant, erosional scarplet, fan piedmont – see coordinates) within the Jornada Experimental Range in July 2021 to answer questions about how these variables change across these spatial scales in drylands. This project was a collaboration between researchers at New Mexico State University and The University of Texas at El Paso as part of the Drylands Critical Zone Thematic Cluster within the Critical Zone Network. This dataset is complete.
Effects of Warming on Soil Microbial Oxidative Activity at Harvard Forest 2013
As Earth’s climate warms, the massive stores of carbon found in soil are predicted to become depleted, and leave behind a smaller carbon pool that is less accessible to microbes. At a long-term forest soil-warming experiment in central Massachusetts, soil respiration and bacterial diversity have increased, while fungal biomass and microbially-accessible soil carbon have decreased. Here, we evaluate how warming has affected the microbial community’s capability to degrade chemically-complex soil carbon using lignin-amended BioSep beads. We profiled the bacterial and fungal communities using PCR-based methods and completed extracellular enzyme assays as a proxy for potential community function. We found that lignin-amended beads selected for a distinct community containing bacterial taxa closely related to known lignin degraders, as well as members of many genera not previously noted as capable of degrading lignin. Warming tended to drive bacterial community structure more strongly in the lignin beads, while the effect on the fungal community was limited to unamended beads. Of those bacterial operational taxonomic units (OTUs) enriched by the warming treatment, many were enriched uniquely on lignin-amended beads. These taxa may be contributing to enhanced soil respiration under warming despite reduced readily available C availability. In aggregate, these results suggest that there is genetic potential for chemically complex soil carbon degradation that may lead to extended elevated soil respiration with long-term warming.
Extracellular polymeric substances are closely related to land cover, microbial communities, and enzyme activity in tropical soils
<p>These are datasets and R codes linked to the paper: Extracellular polymeric substances are closely related to land cover, microbial communities, and enzyme activity in tropical soils. </p>
Soil redox potential vs. soil microbial activity and structure
<p>The dataset was collected as a part of the Carbon Action field experiment in 2021. The experiment had 100 farms testing carbon farming practices 2019-2023. These samples were from a subset of 20 farms, which were routinely sampled every year.</p> <p>I measured the following parameters</p> <ul> <li>redox potential Eh, with an ORP meter + electrode reference voltage at 50% pore filled moisture</li> <li> pH in distilled water 10:1 water:soil mixture</li> <li>VESS soil structure on the field</li> <li>CO2burst by rewetting dried soil and measuring how much CO2 was respired in 24 hours</li> </ul> <p>pH and Eh were used to calculate a rH2 hydrogen potential, which combines Eh and pH</p> <p>Background information for clay and organic matter content was determined separately</p> <p>the clay content was defined by sieving and the organic matter content by loss of ignition.</p> <p>The analysis of the results is published in Plant and Soil journal in 2023. </p> <p>Mattila, T.J., 2023. Redox potential as a soil health indicator - how does it compare to microbial activity and soil structure?</p> <p><a href="https://doi.org/10.1007/s11104-023-06305-y">https://doi.org/10.1007/s11104-023-06305-y</a></p>
Hubbard Brook Experimental Forest: Soil Acid-Base Properties and Microbial Activity, Watershed 1 and West of Watershed 6 (2015-2016)
In summer 2015 and spring 2016, researchers collected soils from the CaSiO3-enriched watershed at Hubbard Brook (W1) and from a nearby site west of the reference watershed at Hubbard Brook (W6). These soils were sampled throughout the hardwood zone of each watershed, and the sampling scheme explicitly examined pit-and-mound microtopographic gradients. These soils were analyzed for acid-base properties, net and gross N cycling rates, microbial biomass, and C cycling rates. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the US Forest Service, Northern Research Station.
Data collected for: The contrasted impacts of grasshoppers on soil microbial activities in function of ecosystem productivity and herbivore diet
<p>Herbivory can have contrasted impacts on soil microbes and nutrient cycling, which has stimulated the development of conceptual frameworks exploring the links between below- and aboveground processes. The "productivity model" predicts that herbivores stimulate microbial activities and accelerate nutrient mineralization in productive ecosystems, while they have an opposite effect in less productive ecosystems. In parallel, the "diet model" predicts that herbivores feeding on conservative plants accelerate nutrient cycling while those feeding on exploitative plants decelerate nutrient cycling, due to changes in litter inputs. Since these two frameworks can lead to conflicting predictions in some cases, experimental evidence combining herbivore diet and productivity is required.</p> <p>During two consecutive years, we conducted an experiment controlling the presence of three grasshopper species consuming either grasses, forbs or both in twelve natural and managed alpine grasslands of contrasted productivities. In order to assess the effects of herbivory on soil microbes, we measured their enzymatic activities, their biomass and the soil potential nitrogen mineralization (PNM). Soil and vegetation characteristics were also determined in order to test if they modulated the effects of herbivory on microbes.</p> <p>Contrary to the predictions of the diet model, the effects of herbivory on microbial characteristics did not depend on the herbivores diet but relied on ecosystem productivity. The most productive sites were characterized by exploitative plant species which depleted N resources in the soil, and by microbes producing relatively few extracellular enzymes, leading to a lower PNM. Herbivory increased microbial biomass and decreased the production of extracellular enzymes in those sites, possibly through the stimulation of root exudates produced by exploitative species. The least productive sites were characterized by conservative plants, which led to the sequestration of soil C, and by microbes having a resource acquisition strategy (more extracellular enzymes, higher PNM). Herbivory decreased microbial biomass and increased the production of extracellular enzymes in those sites. This pattern can be explained by the loss of carbon associated with insect respiration, which increases the need for microbes to acquire resources and by a lower production of root exudates by conservative species. Therefore, the effects of two years of herbivory on soil microbes were at odds with the productivity model, which focuses instead on longer term effects corresponding to herbivory-induced changes in plant species composition. This highlights the multidimensional feature of the impacts of herbivory on ecosystem functioning, both in space and time.</p>
Energetic return on investment determines overall soil microbial activity.
<p><strong>Data</strong> and <strong>R codes</strong> used for the manuscript entitled " <strong>Energetic return on investment determines overall soil microbial activity."</strong></p>
Data collected for: The contrasted impacts of grasshoppers on soil microbial activities in function of ecosystem productivity and herbivore diet
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Inorganic nitrogen, microbial ecoenzymatic activities, and organic matter in soils collected from the Monsoon Rainfall Manipulation Experiment (MRME), Sevilleta National Wildlife Refuge, New Mexico during the 2014 growing season
Drylands are characterized by a pulse dynamics framework in which episodic rain events trigger brief pulses of biological activity and resource availability that regulate primary production in these ecosystems. Relatively small rain events can stimulate microbial processes like decomposition that release inorganic nitrogen needed by plant processes, which typically also depend on soil moisture received from larger rain events. Little is known how changes in rainfall patterns may affect plant available nitrogen in dryland soils, particularly across temporal scales. Therefore, we conducted a study to examine the daily and seasonal responses of plant available nitrogen to rain events that differed in size and frequency throughout a summer monsoon in a northern Chihuahuan Desert grassland located in the Sevilleta National Wildlife Refuge, New Mexico, USA. This data package, which accompanies an associated manuscript (Brown et al. 2022), contains measurements of inorganic nitrogen, nitrogen-acquiring microbial ecoenzymatic activities, and organic matter in soils collected from the Monsoon Rainfall Manipulation Experiment (MRME) during the 2014 summer growing season.
Soil microbial activity and abundance data in the rhizosphere and bulk soils of O horizon in the MELNHE study, 2014
The Multiple Element Limitation in Northern Hardwood Ecosystems (MELNHE) project studies N and P acquisition and limitation through a series of nutrient manipulations in northern hardwood forests. This data set includes rhizosphere processes and microbial abundance at the Bartlett Experimental Forest. Samples were collected once, in summer 2014, in the forth year of fertilization with N and P. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Spatial patterns and effects of invasive plants on soil microbial activity and diversity along river corridors - raw data
<p>Dataset for the study</p> <p><strong><span>Spatial patterns and effects of invasive plants on soil microbial activity and diversity along river corridors</span></strong></p> <ol> <li>environmental variables of the research plots</li> <li>vascular plant species composition of the research plots</li> <li>mcirobial activity on the research plots</li> <li>CLPP profiles of the research plots</li> </ol>
Dataset for manuscript entitled: Switchgrass cropping systems affect soil carbon and nitrogen and microbial diversity and activity on marginal lands
<p class="MsoListParagraph">Switchgrass (<em>Panicum virgatum</em> L.),<span> </span>as a dedicated bioenergy crop, can provide cellulosic feedstock for biofuel production while improving or maintaining soil quality. However, comprehensive evaluations of how switchgrass cultivation and nitrogen (N) management impact soil and plant parameters remain incomplete. We conducted<span> </span>field trials in three years (2016–2018) at six locations in the North Central Great Lakes Region to evaluate the effects of cropping systems (switchgrass, restored prairie, undisturbed control) and N rates (0, 56 kg N ha<sup>-1</sup> yr<sup>-1</sup>) on biomass yield and soil physicochemical, microbial, and enzymatic parameters. Switchgrass cropping system yielded an aboveground biomass 2.9–3.3 times higher than the other two systems (Jayawardena et al., In submission) but our study found that this biomass accumulation didn't reduce soil dissolved organic C (DOC), total dissolved N (TDN), or bacterial diversity. The annual aboveground biomass removal for bioenergy feedstock, however, reduced soil microbial biomass C (MBC) and N (MBN) and bacterial richness in the 2<sup>nd</sup> and 3<sup>rd</sup> years; despite this, continuous monocropping of switchgrass improved soil TDN, inorganic N, bacterial diversity, and shoot biomass in the 2<sup>nd</sup> and/or 3<sup>rd</sup> years when compared to the 1<sup>st</sup> year. N fertilization increased aboveground biomass yield by 1.2 times and significantly increased soil TDN, MBN, and the shoot biomass of switchgrass when compared to the unfertilized control. Locations with higher C and N contents and lower C:N ratio had higher aboveground biomass, MBC, MBN, and the activity of BG, CBH, and UREA enzymes; by contrast, locations with higher pH had higher soil TDN and activity of NAG and LAP enzymes. Our research demonstrates that switchgrass cultivation could improve or maintain soil N content and N fertilization can increase plant biomass yield. The comprehensive data also can inform future biogeochemical models to successfully implement switchgrass for bioenergy production.</p>
Data and metadata of soil microbial community structure, enzyme activities, functional genes and earthworms derived from H2020 Diverfarming project
<p>Soil data and metadata of soil microbial community structure, enzyme activities (dehydrogenase, β-glucosidase, leucine-aminopeptidase, alkaline phosphatase and arylsusfatase activities), N functional genes and earthworms from the different cases studies and long terms from WP4 "Impact of crop diversification on biodiversity", derived from H2020 Diverfarming project. The main objective of workpackage is to provide a scientific understanding of the link between diversified cropping systems, above- and belowground biodiversity, and the resulting ecosystem services provided by soil microorganisms, soil invertebrates and vegetation in agro-ecosystems. Soil organisms contribute to all biogeochemical cycles, Soil organic matter mineralization and stabilization, shape soil structure and have associations with plant species promoting growth and development. http://www.diverfarming.eu.</p>
Biochar and nitrogen fertilizer promote rice yield by altering soil enzyme activity and microbial community structure
<p><span>Biochar can significantly change soil properties and improve soil quality.</span> <span>However, the effects of long-term combined application of biochar (B) and nitrogen (N) fertilizer on relationships between soil enzyme activity, microbial community structure and crop yield are still obscure. We characterized these relationships in a long-term (8 years) field experiment with rice, two biochar rates of 0 and 13.5 t ha<sup>-1</sup> year<sup>-1</sup> (B0 and B) and two N fertilizer rates of 0 and 300 kg N ha<sup>-1</sup> year<sup>-1</sup> (N0 and N).</span><span> The repeated, long-term combined applications of biochar and N fertilizer significantly increased microbial biomass carbon and nitrogen (MBC and MBN), but biochar decreased the abundance of total bacteria, fungi, actinomycetes, Gram-positive and Gram-negative bacteria as well as the amount of total phospholipid fatty acids. </span><span>The activity of leucine aminopeptidase (LAP) </span><span>decreased significantly in the biochar-amended and N fertilized treatment, but</span><span> the LAP activity either remained unchanged or increased with biochar amendment at N0. The relative abundance of bacterial phylum <em>Chloroflexi</em> was increased in the combined biochar and N fertilizer treatment. The changes in soil organic matter and the activity of α-1,4-xylosidase were the major properties influencing soil bacterial community composition, whereas the structure of fungal community was governed by MBC, MBN and LAP activity. In addition, long-term biochar and N fertilizer applied together significantly increased rice yield (more than biochar and nitrogen fertilizer applied alone). Yield</span> <span>was significantly positively correlated with LAP activity, but significantly negatively correlated with the relative abundance of Chloroflexi. In conclusion, long-term biochar and nitrogen fertilizer applications increased rice yield, which was associated with altered soil microbial community and enhanced activity of some enzymes.</span></p>
Dataset for manuscript entitled: Switchgrass cropping systems affect soil carbon and nitrogen and microbial diversity and activity on marginal lands
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Plant invasions alter soil biota and microbial activities: A global meta-analysis
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Biochar and nitrogen fertilizer promote rice yield by altering soil enzyme activity and microbial community structure
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Soil microbial functions and enzyme activity:BAC: Biodiversity and Climate
Climate changes forecast for our region by GCM???s and shifts in biodiversity and composition each have the potential to alter ecosystem functioning; their interactive effects are unknown. The "BAC" experiment is designed to determine the direct and interactive effects of plant species numbers, plant community composition, temperature, and precipitation on 11 productivity, C and N dynamics, stability, and plant, microbe, and insect species abundances in CDR grassland ecosystems.
Data from: Microplastics negatively affect soil fauna but stimulate microbial activity: insights from a field-based microplastic addition experiment
<p>Microplastics are recognized as an emerging contaminant worldwide. Although microplastics have been shown to strongly affect organisms in aquatic environments, less is known about whether and how microplastics can affect different taxa within a soil community, and it is unclear whether these effects can cascade through soil food webs. By conducting a microplastic manipulation experiment, i.e. adding low-density polyethylene fragments in the field, we found that microplastic addition significantly affected the composition and abundance of microarthropod and nematode communities. Contrary to soil fauna, we found only small effects of microplastics on the biomass and structure of soil microbial communities. Nevertheless, structural equation modeling revealed that the effects of microplastics strongly cascade through the soil food webs, leading to the modification of microbial functioning with further potential consequences on soil carbon and nutrient cycling. Our results highlight that taking into account the effects of microplastics at different trophic levels is important to elucidate the mechanisms underlying the ecological impacts of microplastic pollution on soil functioning.</p>
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