Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
5
datasets available to search
ShareScore release 0.9.0
Dataset results
5 results for “spectrophotometric assay”
Spectrophotometric Assay for the Detection of 2,5-Diformylfuran and Its Validation through Laccase-Mediated Oxidation of 5-Hydroxymethylfurfural
<p>Modern biocatalysis requires fast, sensitive, and efficient high-throughput screening methods to screen enzyme libraries in order to seek out novel biocatalysts or enhanced variants for the production of chemicals. For instance, the synthesis of bio-based furan compounds like 2,5-diformylfuran (DFF) from 5-hydroxymethylfurfural (HMF) via aerobic oxidation is a crucial process in industrial chemistry. Laccases, known for their mild operating conditions, independence from cofactors, and versatility with various substrates, thanks to the use of chemical mediators, are appealing candidates for catalyzing HMF oxidation. Herein, Schiff-based polymers based on the coupling of DFF and 1,4-phenylenediamine (PPD) have been used in the set-up of a novel colorimetric assay for detecting the presence of DFF in different reaction mixtures. This method may be employed for the fast screening of enzymes (Z' values ranging from 0.68 to 0.72). The sensitivity of the method has been proved, and detection (8.4 μM) and quantification (25.5 μM) limits have been calculated. Notably, the assay displayed selectivity for DFF and enabled the measurement of kinetics in DFF production from HMF using three distinct laccase–mediator systems.</p>
Spectrophotometric and Fluorimetric High-Throughput Assays for Phenolic Acid Decarboxylase
<p>Biocatalytic decarboxylation of hydroxycinnamic acids yields phenolic styrenes, which are important precursors for antioxidants, epoxy coatings, adhesives and other polymeric materials. <em>Bacillus subtilis</em> decarboxylase (<em>Bs</em>PAD) is a cofactor-independent enzyme that catalyzes the cleavage of carbon dioxide from <em>p</em>-coumaric-, caffeic-, and ferulic acid with high catalytic efficiency. Real-time spectroscopic assays for decarboxylase reactions remove the necessity of extensive sample workup, which is required for HPLC, mass spectrometry, gas chromatography, or NMR methods. This work presents two robust and sensitive assays based on photometry and fluorimetry that allow decarboxylation reactions to be followed with high sensitivity while avoiding product extraction and long analysis times. Optimized assay procedures were used to measure <em>Bs</em>PAD activity in cell lysates and to determine the kinetic constants (<em>K</em><sub>M</sub> and <em>V</em><sub>max</sub>) of the purified enzyme for <em>p</em>-coumaric-, caffeic- and ferulic acid. Substrate inhibition was shown for caffeic acid.</p>
Green spectrophotometric analysis data for the assay of betrixaban and lercanidipine
<p><span>Three facile, accurate, and eco-friendly spectrophotometric methods were developed for determination of the novel anticoagulant drug, betrixaban (BTX). The first method (method A) was based on the unique UV absorption spectrum of BTX, where direct analysis of BTX was performed at 229.4nm on the recorded zero order spectrum using methanol as the optimum solvent. While the second method(method B) was based on measuring difference absorption value(DA) of BTX at 335nm, which was obtained from pH-induced spectral difference (Difference spectra of BTX in 0.1M NaOH <i>versus</i> 0.1M HCl). The third mathematical spectrophotometric method (method C) was established for simultaneous analysis of BTX with the co-administered Ca channel blocker lercanidipine (LER). In this method, the first order derivative technique was utilized to resolve the overlapping UV spectra of both drugs. The first derivative amplitudes at 206nm and 229nm were used for quantitative assay of BTX and LER, respectively. All methods obeyed Beer<span>'</span>s law in the concentration ranges of 1.0–20.0µg.mL<sup>-1</sup> and 8.0–80.0 µg.mL<sup>-1</sup> for BTX in methods A and B, respectively, and of 1.0–20.0 µg.mL<sup>-1</sup> and 1.0–25.0µg.mL<sup>-1</sup> for BTX and LER, respectively in method C. The three methods were fully validated, and the results showed good agreement with the other compared ones where high %recoveries and low values of RSD (<1.5%) were obtained. Moreover, the green profile of the proposed methods was assessed by using two approaches: analytical eco-scale and green analytical procedure index. The results indicated that method B was the greenest one because hazardous organic solvents were avoided. Both methods A and C have analytical eco-scale scores of 85 which indicated the greenness of these methods as well. The absence of interferences that could be produced from encountered additives, excipients, or degradation products ensured the high specificity of the proposed methods and supported their applicability in quality control laboratories. </span></p>
Design and development of spectrophotometric enzymatic cyanide assays_SI
Open the record for dataset details and reuse information.
Green spectrophotometric analysis data for the assay of betrixaban and lercanidipine
Open the record for dataset details and reuse information.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.